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  • CM Urine Chem Exam Summary

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    Jasmine Medina
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    This document provides information on urine test strips, including the principle, reagent, and positive color for various parameters including glucose, bilirubin, ketones, specific gravity, proteins, pH, blood, urobilinogen, nitrite, and leukocyte esterase. The parameters are tested using enzyme reactions, diazo reactions, or indicator changes in response to pH. Positive results are indicated by color changes ranging from blue to green to brown, tan or pink to violet, purple, blue to green, red to yellow, yellow to blue, uniform blue/green or speckled/spotted, cherry red, and purple. Test times range from 30 to 120 seconds.

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    This document provides information on urine test strips, including the principle, reagent, and positive color for various parameters including glucose, bilirubin, ketones, specific gravity, proteins, pH, blood, urobilinogen, nitrite, and leukocyte esterase. The parameters are tested using enzyme reactions, diazo reactions, or indicator changes in response to pH. Positive results are indicated by color changes ranging from blue to green to brown, tan or pink to violet, purple, blue to green, red to yellow, yellow to blue, uniform blue/green or speckled/spotted, cherry red, and purple. Test times range from 30 to 120 seconds.

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    6 views1 page

    CM Urine Chem Exam Summary

    Uploaded by

    Jasmine Medina
    This document provides information on urine test strips, including the principle, reagent, and positive color for various parameters including glucose, bilirubin, ketones, specific gravity, proteins, pH, blood, urobilinogen, nitrite, and leukocyte esterase. The parameters are tested using enzyme reactions, diazo reactions, or indicator changes in response to pH. Positive results are indicated by color changes ranging from blue to green to brown, tan or pink to violet, purple, blue to green, red to yellow, yellow to blue, uniform blue/green or speckled/spotted, cherry red, and purple. Test times range from 30 to 120 seconds.

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    Urine Parameter Principle Reagent Positive Color


    Time (s)
    30 Glucose Double Glucose Oxidase Blue to Green to Brown
    Sequential Peroxidase (Multistix)
    Enzyme Reaction Potassium Iodide (Multistix)
    Tetramethylbenzidine Yellow to Green
    (Chemstrip) (Chemstrip)
    Bilirubin Diazo Reaction 2,4 dichloro… (+) Tan or pink to violet
    Aniline (multistix)
    Benzene (chemstrip)
    40 Ketones Sodium Sodium Nitroprusside/ (+) Purple
    Nitroprusside nitroferricyanide + Glycine
    Reaction (Legal’s (Chemstrip)
    Test)
    45 S.G. pKa (dissociation Mahaba+… Blue (1.000) to
    constant) change bromthymol blue Yellow (1.030)
    of polyelectrolyte
    60 Proteins Protein Tetrabrom… (+) Blue to Green
    (Sorensen’s) Error Tetrachlor… (-) Yellow
    of Indicators Tetrabrom…
    Citrate buffer at pH 3
    pH Double Indicator Methyl Red
    System - pH 4 – 6; Red to Yellow
    - pH 5 = Orange
    Bromthymol Blue
    - pH 6 – 9; Yellow to Blue
    Blood Pseudoperoxidase Mahaba + …tetramethylbenzidine Uniform Blue/ Green →
    activity of Hemoglobin
    hemoglobin
    Speckled/spotted → intact
    RBCs
    Urobilinogen Ehrlich reaction PDAB (Multistix) (+) Cherry Red
    (non-specific) 4-methoxybenzene…tetrafluoro-
    borate (Chemstrip; specific for
    UBG)
    Nitrite Greiss Reaction …quinoline quinoline quinoline (+) Uniform Pink
    120 Leukocyte Esterase Leukocyte Pyrrole amino acid ester (+) Purple
    esterase (Multistix)
    Indoxylcarbonic acid ester
    (Chemstrip)

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