Homework # 3

L A B O R A T O R Y
T E C H N I Q U E S
Q1. What is Pipetting?

A laboratory technique that includes

the accurate measuring and
transferring of a sample or a reagent.

(1) Forward pipetting is a technique

primarily recommended for aqueous
solutions, such as buffers, or dilute
acids or alkalis.

(2) Reverse pipetting is used for

solutions with high viscosity or a
tendency to foam as it minimizes the
risk of splashing, foam, or bubble
formation. Reverse piping is more
accurate in dispensing small amounts
of liquids containing proteins and
biological solutions. Q2. Differentiate TC Pipettes
from TD Pipettes.
When a piece of glass is calibrated to deliver a specific volume, it is referred to as "to deliver"
(TD) glass, while a piece of glass is calibrated to contain a specific volume, it is referred to as "to
contain" (TC).

TD

TD

TC

Group 7 | BSN 1102L

Page 1
Homework # 3
L A B O R A T O R Y
S A F E T Y
Q3. Enumerate the different
types of Fire Extinguishers.
The different types of fire extinguishers are water extinguisher, carbon dioxide extinguisher, , powder
fire extinguisher, foam extinguisher, CO2 fire extinguisher, powder extinguisher, carbon dioxide
extinguisher, and halon extinguisher.

Q4. How are hazardous

chemicals group in the lab?
Hazardous chemicals are grouped and categorized in the laboratory based on their properties and potential
risks to us humans and the environment to ensure the safety in the laboratory as well as the waste disposal.
According to the Global Harmonized System (GHS) there are 3 classifications of hazards; Physical, Health, and
Environmental Hazard.

Group 7 | BSN 1102L

Page 2