Exercise 7.

1 Enzyme Concentration and Rate of Starch Hydrolysis

Table 7.1A
Transmittance (%T620) at Time (sec)
Amylase
Concentration 0 30 60 90 120 150 180 210
(U/mL)

8 5.5 10.0 14.0 17.0 22.5 24.0 28.5 31.0

4 4.5 6.0 7.5 9.0 10.0 11.0 14.0 15.0
2 5.5 6.5 7.0 8.0 9.0 9.5 10.0 11.0

Table 7.2A
Transmittance (%T620) at Time (sec)

Starch
Concentration (%) 0 30 60 90 120 150 180 210

0.500 5.5 10 14 17 22.5 24 28.5 31

0.250 5.5 8.5 12.5 16 20 24 28 32
0.125 13.5 17.5 21.5 25.5 30
Table 7.3A Table 7.3B
Transmittance (%T620) at pH
Time (sec) 5 6 7
pH 0 300 Slope (m) 0.157 0.270 0.295
5 4 51
6 3.5 84.5
7 3.5 92
8 5.5 74.5

Conclusion: Fit conclusion in this space!

Table 7.4A Table 7.4B

Transmittance (%T620) at Temperature (°C)
Time (sec) 24 35 42
Temp (°C) 0 300 Slope (m) 0.2950 0.3083 0.3050
24 3.5 92
35 2 94.5
42 3 94.5
56 3.5 55.5

Conclusion: Fit conclusion in this space!

Conclusion: Fit conclusion in this space!
 Table 7.1B Graph 7.1B
c) Enzyme Concentration (U/mL)
8 4 2
240 270 300 Slope (m) 0.1127 0.0479 0.0242

34.0 37.0 39.5

16.0 17.0 19.0
11.5 12.0 13.0

Conclusion: An increased enzyme concentration causes the speed of reactions to catalyze quick

***I couldn't figure out how to change the series names without messing up the data so I left t

Table 7.2B Graph 7.2B

c) Enzyme Concentration (U/mL)
0.500 0.250 0.125
240 270 300 Slope (m) 0.113 0.126 0.145

0.160
34 37 39.5 0.140
35.5 39 42.5 Transmittance (%T620) 0.120
35.5 39.5 43.5 0.100
0.080
0.060
0.040
0.020
0.000
Conclusion: As the substrate (starch in this case) concentration increases, the rate of the reacti
 Graph 7.3B
H
8 Effect of pH on the Rate of Starch Hydrolysis
0.230 0.350

0.300

0.250

0.200
Axis Title

0.150 Fit graph in this space!

0.100

0.050

0.000
1 2 3 4

Axis Title

Row 46 Linear (Row 46)

****x-axis labels respectively: 5, 6, 7, and 8.

Graph 7.4B
ture (°C)
56
Effect of Temperature on the Rate of Starch Hydrolysis
0.1733 0.3500

0.3000

0.2500

0.2000
Axis Title

0.1500 Fit graph in this space!

0.1000

0.0500

0.0000
1 2 3 4

Axis Title

Row 66 Linear (Row 66)

 0.0000
1 2 3 4

Axis Title

Row 66 Linear (Row 66)

****x -axis labels respectively: 24, 35, 42, and 56.

Graph 7.1B

Enzyme Concentration and the Rate of Starch Hy-

drolysis
0.1200

0.1000 f(x) = − 0.0442424242424243 x + 0.15010101010101

Transmittance (%T620)

0.0800

0.0600
Fit graph in this space!
0.0400

0.0200

0.0000
8 4 2

Amylase Concentration (U/mL)

Row 6 Linear (Row 6)

g up the data so I left them. But the first one is 8, the second is 4 and the third is 2.

Graph 7.2B

Substrate Concentration and the Rate of Starch

Hydrolysis

0.160
0.140
Transmittance (%T620)

0.120
0.100
0.080 Fit graph in this space!
0.060
0.040
0.020
0.000
1 2 3

Row 26 Linear (Row 26)

****x-axis labels respectively: 0.500, 0.250, and 0.125.