Lock and Key

&
Induced Fit
rate Interactions
Enzyme Subst
 Lesson
Outcomes
Identify the basic parts of an enzyme,
including substrate, and active site.

Identify and differentiate the two models of

enzyme interactions

Understand the concept of Lock and Key

Model and Induced Fit Model
 Introduction to Enzymes
Enzymes
Protein based catalysts.
Increase the rate of a chemical reaction without
being changed themselves in the process.
Very Specific to a substrate
They can be denatured by changes in pH and
temperature
Enzymes generally have a cleft for active site,
generally <5% of surface)
 Introduction to Enzymes
Active Site
the region that binds the substrate and
converts it into product
a three-dimensional entity, often a cleft
on the surface of the protein
has various amino acid residues

Substrate
molecule upon which an enzyme acts in
a chemical reaction
 Introduction to Enzymes
Active site

Substrate

Enzyme Enzyme-substrate
complex
Products
Enzyme-Substrate Interactions
Two models have been proposed to explain how an enzyme binds its substrate:

Lock and Key Induced Fit

 Lock and Key Theory
1894
Emil Fischer
The shape of the substrate and the active
site of the enzyme are thought to fit
together like a key into its lock

active site has a rigid and fixed shape

active site has same shape as substrate
Lock and Key Model Emil Fischer
Lock and Key Model
 Induced Fit Theory
1958
Daniel Edward Koshland Jr.
The binding of substrate induces a conformational
change in the active site of the enzyme

active site don't have a rigid and fixed shape

enzyme has flexible active site
the enzyme may distort the substrate, forcing it
into a conformation similar to that of the
transition state Example: Daniel Edward
Hand fit to the Glove Koshland Jr.
Induced Fit Model
Lock and Key

Vs

Induced Fit
Reference:
https://pharmabookbank.files.wordpress.com/2019/03/6.1.biochemistry.pdf

Reporter:
Maulana, Nhorhoney L. (BSPh 2B)