Makenzie Bailey

620147606
Lab partners:
October 12, 2022
Chlorophyll and photosynthesis
Aim: To determine the effect of light intensity and chlorophyll presence on photosynthesis of
Hibiscus and Bougainvillea leaves via a starch test and to measure photosynthesis and respiration
rates in an Elodea plant.

Method: As seen in BIOC2014 manual pages 50-53

Results:

TABLE 1: INITIAL AND FINAL VOLUME IN CYLINDERS CONTAINING ELODIA

EXPOSED TO LIGHT AND WITHOUT LIGHT AND CYLINDERS CONATINING
NO ELODIA BUT EXPOSED TO LIGHT AND WITHOUT LIGHT

Measuring cylinder Initial volume ml Final volume ml

containing

Elodia + light 50 51
Elodia + no light 50 51

No Elodia + light 50 50

No Elodia + no light 50 50
TABLE 2 INITAL, FINAL AND USED VOLUME OF NaOH USED IN A
NEUTRALIZATION REACTION WITH CYLINDERS CONTAING ELODIA
EXPOSED TO LIGHT VS DARK AND NO ELODIA EXPOSED TO LIGHT VS
DARKALONG WITH THE NUMBER OF DROPS OF NAOH

Measuring Initial volume of Final volume of Volume of Number of

cylinder NaOH NaOH NaOH used drops of
containing NaOH
Elodia + light 5.70 6.60 0.90 18
Elodia + no light 6.60 8.58 1.98 36

No Elodia + light 8.58 10.39 1.81 34

No Elodia + no 10.39 12.82 2.43 50
light
Drawings can be seen on the next page

Calculations
D= no. of drops of 0.02 N NaOH required to titrate darkened sample with plant
L= no. of drops of 0.02 N NaOH required to titrate lighted sample with plant
N= no. of drops of 0.02 N NaOH required to titrate sample without plant
V= volume of plant (ml)
T= duration of experiment (hrs)

Sample calculation
Rate of photosynthesis
D – L = micromoles of CO2 absorbed/ mL. plant/hour
VxT
36–18= micromoles of CO2 absorbed/ mL. plant/hour
1 x 1.5
= 12 micromoles of CO2 absorbed/ mL. plant/hour

Rate of respiration

D – N =micromoles CO2 evolved/ ml. plant/hour

VxT
36– 50 =micromoles CO2 evolved/ ml. plant/hour
1 x 1.5

= -9.3 micromoles CO2 evolved/ ml. plant/hour

TABLE 2 INITAL, FINAL AND USED VOLUME OF NaOH USED IN A

NEUTRALIZATION REACTION WITH CYLINDERS CONTAING ELODIA
EXPOSED TO LIGHT VS DARK AND NO ELODIA EXPOSED TO LIGHT VS
DARKALONG WITH THE NUMBER OF DROPS OF NAOH

Measuring Initial volume of Final volume of Volume of Number of

cylinder NaOH NaOH NaOH used drops of
containing NaOH
Elodia + light
Elodia + no light

No Elodia + light
No Elodia + no
light