Agrobacterium-mediated Genetic Transformation

Agrobacterium-mediated genetic transformation is the dominant technology used for the

production of genetically modified transgenic plants. Agrobacterium-mediated gene transfer is
known to yield marker-free plants, which is a need for the commercialization of transgenic crops.
It often results in the integration of foreign genes at a single locus in the host plant, without
accompanying vector backbone. Various commercially and horticulturally significant monocot
and dicot species have been successfully transformed by Agrobacterium using both conventional
tissue culture and in planta transformation procedures such floral or seedling penetration, apical
meristem transformation, and the pistil drip methods. The genotype of the host plant, the explant,
the vector, the plasmid, the bacterial strain, the composition of the culture medium, tissue
damage, and the temperature of co-cultivation are just a few of the variables that affect the
highly effective transformation process in plants that is mediated by agrobacterium. (Mehrotra &
Goyal, 2012)

Sr. Crop Name Gene Name Function Citation

No
1 Orange ERF109 It contributes to cold (Wang et al., 2019)
tolerance by directly
regulating expression of
Prx1 involved
inantioxidative process
2 Cotton Cry1Ac It encodes a toxin highly (Singh et al., 2016)
effective against many
lepidopteran pests.
3 Tomato Cry1Ab It confers resistance to the (Koul et al., 2014)
plant to lepidoptera larvae
4 Soybean rhg1 It contributes to resistance (Cregan et al., 1999)
against soybean cyst
nematode.
5 Corn Cry9C This protein has pesticidal (Hilbeck & Andow,
 properties & protect the 2002)
crop against several insects.
6 Potato Rpi-vnt1 It encodes the VNT1 (Habig et al., 2018)
protein, protects against late
blight, caused by
Phytophthorainfestans.
7 Papaya prsvcp A process related toviral (Chiang et al., 2001)
cross-protection, transgenic
lines with this gene exhibit
resistance to infection and
subsequent disease caused
by PRSV.
8 Apple anti-PPO It blocks the production of (Bajwa et al., 2015)
PPO and therefore stops the
browning.
9 Rice BCO1 It catalyzes the oxidative (Feschotte, 2005)
cleavage of beta,beta-
carotene into two retinal
molecules.

10 Purple Tomatoes Anthocyanins It is antioxidant compounds (Gonzali & Perata,

with widely-recognized 2020)
health benefits.
11 Tobacco 2G12 It broadly neutralizing anti- (Sack et al., 2015)
human immunodeficiency
virus type 1.
12 Grapes VaSAP15 Its function in cold stress. (Shu et al., 2021)
13 Strawberry FaPYR1 It involved in fruit ripening (Chai et al., 2011)
14 Wheat TaCRK2 Contribute to leaf rust (Zheng et al., 2020)
resistance inwheat
15 Barley PKABA1 It suppressed the gene (Li et al., 2007)
 expression in barley
16 Brassica CDAG1 It results in enlarged organ (Karamat et al.,
size and increased biomass. 2021)
17 Grapes SAP15 It exhibited specific tissue (Shu et al., 2021)
expression in different grape
genotypes.
18 Peanut AVP1 It improved drought and salt (Qin et al., 2012)
tolerance.
19 Cotton IPT It improved salt tolerance (Zhu et al., 2018)
and delay leaf senescence.
20 Potato DREB1B Enhance drought and (Wei et al., 2016)
freezing tolerance
References
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Chai, Y. M., Jia, H. F., Li, C. L., Dong, Q. H., & Shen, Y. Y. (2011). FaPYR1 is involved in
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Chiang, C. H., Wang, J. J., Jan, F. J., Yeh, S. D., &Gonsalves, D. (2001). Comparative reactions
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Cregan, P. B., Mudge, J., Fickus, E. W., Danesh, D., Denny, R., & Young, N. D. (1999). Two
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Feschotte, C. (2005). DNA-binding specificity of rice mariner-like transposases and interactions

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Singh, A. K., Paritosh, K., Kant, U., Burma, P. K., &Pental, D. (2016). High Expression of
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Wei, T., Deng, K., Gao, Y., Liu, Y., Yang, M., Zhang, L., Zheng, X., Wang, C., Song, W., Chen,
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