Product datasheet

Bile Acid Assay Kit (Colorimetric) ab239702

5 References 2 Images

Overview

Product name Bile Acid Assay Kit (Colorimetric)

Detection method Colorimetric
Sample type Saliva, Urine, Serum, Plasma
Assay time 1h 20m
Species reactivity Reacts with: Mammals
Product overview Bile Acid Assay Kit (Colorimetric) ab239702 provides a simple, sensitive, and high-throughput
suitable approach to quantify the concentration of total bile acid in biological fluids.

The bile acid assay is based on an enzymatic cycling method in the presence of NADH and a
chromophore. The reduction of the chromophore produces a stable colorimetric product the
absorbance of which can be followed kinetically at 405 nm on a multi-well spectrophotometer.
This absorbance is directly proportional to the amount of total bile acids in the sample.

Other metabolites found in biofluids do not interfere with the assay.

The assay can detect as little as 1 µM of Bile Acids in a variety of samples.

Bile acid assay protocol summary:

- add samples and standards to wells
- add probe mix and incubate at 37°C for 10 min
- add reaction mix
- measure absorbance at 405 nm for 60 min at 37°C in a kinetic mode

Notes This product is manufactured by BioVision, an Abcam company and was previously called K209
Total Bile Acids (TBA) Assay Kit (Colorimetric). K209-100 is the same size as the 100 test size
of ab239702.

Platform Microplate reader

Properties

Storage instructions Store at -20°C. Please refer to protocols.

1
 Components 100 tests

NADH 1 vial

TBA Cycling Assay Buffer 1 x 7ml

TBA Cycling Enzyme Mix 1 vial

TBA Probe 1 vial

TBA Probe Buffer 1 x 14ml

TBA Standard (100 mM) 1 vial

Relevance Bile Acids (BA) make 67% of the total composition of Bile. They are 24-carbon steroids
generated during cholesterol metabolism. They form conjugates with either glycine or taurine to
form bile salts. Five of the BAs account for more than 99% of the total population found in
biofluids. The average composition in healthy individuals includes conjugates of cholic,
chenodeoxycholic, deoxycholic and lithocholic acids. Bile acids are critical due to their ability to
solubilize lipids by forming micelles with cholesterol, and fatty acids. Their synthesis is not only
critical for the removal of cholesterol from the body abut they are also needed for proper uptake of
dietary lipids into the small intestine.

Images

Bile Acid Assay Standard Curve.

2
 30 µL of each undiluted sample was assayed following the kit
protocol. Bile Acids concentrations are: Serum (in µM): A: 3.56 ±
0.41, B: 2.41 ± 0.27, C: 1.63 ± 0.17, D: 1.34 ± 0.25, Urine: 0.16 ±
0.02 µM/mM Creatinine.

Estimation of Bile Acid concentration in human

serum and urine.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"

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