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Pre Tutorial3 - Translation
Pre Tutorial3 - Translation
Unit 3.4
Learning Objectives
Base pairing
determines the
relationship
between DNA and
mRNA and tRNA
Gene Expression
1. Transcription
2. Translation
/coding strand
Base pairing
determines the
relationship
between DNA and
mRNA and tRNA
RNA is translated into a protein beginning at the start
codon and ending at the stop codon
Ribosome Binding Site
codons
Codon
sequence
CGU CGC CGA CGG AGA AGG
(on
mRNA)
Anti-
codon
GCA GCG GCU GCC UCU UCC
sequence
(on tRNA)
mRNA 5’ – C G U – 3’
Note: the tRNA image
tRNA 3’ – G C A – 5’ anti-codon is shown
5’ ® 3’ = 5’ ACG 3’
tRNA Wobble Effect
Codon 5’ 3’
CGU CGC CGA CGG AGA AGG
(mRNA)
Anti-
3’ 5’
codon GCA GCG GCU GCC UCU UCC
(tRNA)
For the 1st four codons – each share the same 2 bases C and G
i.e. 5’ CG-X 3’
Anti-
codon 3’GCA5’ GCG GCU GCC UCU UCC
(tRNA)
5’– TTAATATGATCTTCGACTAGCTACAGCATTT – 3’
3’– AATTATACTAGAAGCTGATCGATGTCGTAAA – 5’
§ Remember you are answering your own Canvas quiz, you do not
have to provide the answer that the group believes is right if
you think it is wrong
§ You will have about 25 minutes for 4 questions (~6 minutes per
question) to complete the quiz before your breakout groups
end