RESULTS AND DISCUSSION:

1.1. Absorption spectrum:

𝝀𝒎𝒂𝒙 = 538 (nm)

Absorbance (A)

Wavelength (nm)

Figure 1: Absorbance spectrum of 0.5 ppm nitrite solution at pH ~ 8.0 – 8.5

From Figure 1, absorbance was measured at wavelength 400 nm to 800 nm. The
curve reaches the peak of 538 (nm), therefore 𝜆𝑚𝑎𝑥 = 538 (nm) will be used to
measure the absorbance of samples at different concentrations for plotting the
standard calibration curve.

1.2. The standard calibration curve:

Using the UV-Vis spectrophotometer to analyze the absorbance of samples
with the concentration 0, 1, 2, 3, 4 (ppm), respectively at 𝜆 = 538 (nm) to obtain
the results in Table 1.
Table 1. Absorbance of water sample at different concentrations

Concentration (ppm) 0 1 2 3 4

Absorbance 0 0.0382 0.0765 0.1052 0.1597

 0.16

0.14

0.12

Absorbance (A)
0.1

0.08

0.06
y = 0.0386x - 0.0014
R² = 0.9913
0.04

0.02

0
0 1 2 3 4
Concentration (ppm)

Figure 2: Calibration curve illustrates the relationship between

the absorbance and nitrite concentration

Figure 2 shows the relationship between the absorbance and nitrite

concentration by a straight line with the equation is expressed by: y = 0.0386x –
0.014 in which y is absorbance (A) and x is concentration (ppm). Since R 2 =
0.9913, the equation mentioned above completely follows Lambert – Beer’s law.
Thus, this result can be applied to calculate the concentration of nitrite ion in a
meat sample.

1.3. Calculation:

𝐴1 + 𝐴2 + ⋯ + 𝐴𝑛
𝐴̅ =
𝑛

𝐴̅ + 0.014 6 (25 × 10−3 ) × 69 × 10−3 𝑚𝑔

𝐶 𝑁𝑎𝑁𝑂2 𝑖𝑛 𝑠𝑎𝑚𝑝𝑙𝑒 = × × (𝑝𝑝𝑚 𝑜𝑟 )
0.0386 100 5.000 × 10−3 𝑘𝑔