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Гистология, цитология, эмбриология
Гистология, цитология, эмбриология
Гистология, цитология, эмбриология
Page 1 of 125
Histology, cytology, embryology. Practical manual for foreign student’s faculty (course of studies in
English)
Student ________________________________________________________________________group_________.
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TOPIC: MICROSCOPIC AND HISTOLOGICAL TECHNIQUE.
CELL MORPHOLOGY: INTRACELLULAR STRUCTURES
Definition: Histology is
1. Tasks, objects and methods of modern histology. ____________________________________
2. Methods of histological investigations.
___________
3. Techniques of the histological preparation making
4. The principles and methods of dying histological preparations.
Basophylia and oxyphilia (acidophylia)
5. . Definition of a “cell”. Cell derivatives. Cellular theory. _______________________________________________
6. Structural organization of cellular synthetic apparatus:
ribosomes, polyribosomes, endoplasmic reticulum, Golgi
complex. Definition of a
7. Structural organization of cellular digestion apparatus: “CELL”___________________________________
endosomes, lysosomes, peroxysomes.
8. Structural elements of cellular energy: mitochondria
9. Cellular inclusions: classifications, structure and functions.
10.Nucleus and its components
_______________________________________________
11.Cell cycle
Page 3 of 125
Task 1. BIOLOGICAL MICROSCOPE WHEN WORKING WITH THE MICROSCOPE,
Mark in the picture: REMEMBER TO:
1 – stand;
2 – drawtube holder; 1. Put the preparation on the object stage with the cover
3 – drawtube; glass upwards.
4 – objective revolver;
5 – object stage; 2. At low magnification of the microscope use the
6 – micrometer focusing knob; macrometer focusing knob only
7 – condenser;
8 – ocular; 3. Lower the drawtube so that not to damage the
9 – objectives; preparation.
10 – condenser;
11 – mirror; 4. Use the micrometer focusing knob only at high
12 – macrometer focusing magnification and revolve it no more than 5 points on
knob
the scales
5. After the completion of the work at high magnification,
first convert the microscope to low magnification and only
then take the preparation off the object stage.
Task 2. SERIES OF DIAGRAMS
ILLUSTRATING THE APPEARANCE OF
SECTIONS OF STRAIGHT TUBE CUT IN
VARIOUS PLANES
A,B,C – longitudinal sections cut at different
levels relative to the center of lumen. Sections C
not disclose the presence of a lumen.
D –transverse or cross section.
E – Oblique sections.
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PATTERN OF BASIC STAGES OF MAKING HISTOLOGICAL PREPARATIONS
To preserve the structural relationship between cells in tissues, it is necessary to cut very thin slise of tissue, called
section, that are suitable for light or electron microscopy
Process Purpouse Process Purpouse
For light microscopy take a 4. Embedding in paraffin To obtain thin sections with
1. Taking material sample of 1 х 1 х 0,5 sm, for Tissue block must be included in microtome, tissues must be
electron microscopy – 1 х 1 suitable medium – paraffin wax or infiltrated with embedding
substance that impart a rigid
plastic resin consistency to the tissue.
2. Fixation 5.Preparation of histological Sections cut for light
А. Chemical: simple fixators; The process of fixation sections with a microtome microscopy must be thin
compound prevents a tissue by enough to transmit sufficient
fixators;
denaturing its proteins, light, thinner than the
B. Physical: freezing
heating preserve the structure and diameter of most cells (5-10
drying harden soft tissue. µm thin — for transmission
microwave treatment light microscopy, 0,05-0,1
µm – for transmission
electron microscopy)
.
3. Dehydration 6. Staining, impregnating, The methods are chosen
The fixed and rinsed samples The water must be extracted contrasting histological according to the objective of
are passed through increasing from the samples. preparations investigation
strengths of alcohol ( from
70% to100%). The material 7. Place the section between
is kept in each of them from the cover glass adding a
12 to 24 hours. drop of Canadian balm
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STAINING. Task 3. CELLULAR Task 4. SCHEME OF EUCARIOTIC CELL STRUCTURE
The stain routinely used for STRUCTURE
histological sections are the Staining
hematoxylin and eosin. Hematoxylin Hematoxylin-eosin
(H) is basic stain, eosin (E) is acid Magnification
stain. Substances that stain by H are 400Х
basophilic, E – acidophilic
(eosinophilic).
The results of staining test: basophile
cell structures (nucleus, etc.) obtain a
blue or violet coloring after
hematoxylin treatment; acidophilic
structures (cytoplasm, protein granules)
obtain a pink or red coloring after
eosine treatment.
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Task 5. DIAGRAM of COMPLEX GOLDGI Task 7. DIAGRAM OF MITOCHONDRIA
Designate:
1. cis face; A B
2. trans face;
3. Goldgi saccule;
4. transfer vesicles;
5. secretory granules.
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Task 8. LYSOSOMES, PHAGOLYSOSOMES AND OF AXOLOTLE
MULTIVESICULAR BODIES Staining: Hematoxilin-eosin
Magnification: 400х
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Topic: CELL MORPHOLOGY: STRUCTURAL ORGANIZATION OF CELLULAR SURFACE
1. Membrane principle of cell organization. Molecular structure of plasmatic
membrane. Basic organization principles of cell membranes. Task 11. SCHEME OF THE STRUCTURAL AND
2. Free cell surface structure: glycocalyx. Submembraneous components of MOLECULAR ORGANIZATION OF PLASMATIC
cells. MEMBRANE (plasmalemma)
3. Cell receptors, their classification, structural organization. Ion channels.
4. Types of substance transmission through the plasmalemma: endocytosis,
exocytosis
5. Cytoskeleton morphology: microfilaments, microtubules, intermediated
filaments
6.
7. , The structure of special cell organelles: microvilli and cilia
8. Morphology of cell contact surfaces. Classification and structure of
intercellular contacts.
Functions of plasmalemma
1.______________________________________
2._______________________________________
3._______________________________________
4._______________________________________
5._______________________________________
Designate:Ι - glycocalyx, ΙΙ- plasmalemma, ΙΙΙ-
submembrane complex.
1. Correct tasks ___________________
1 – phospholipid bilayer ;
2. Teacher 2 – membrane proteins : а – receptor protein, b – integral
Date «____» _____________ ______ . protein, c – submembrane proteins;
3 – glycoproteins;
4 – glycolipids;
5 – actin microfilaments;
6 – microtubules.
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Designate:
1 – mitochondrion;
2 – endoplasmatic
reticular cistern;
3 – microtubules;
4 – trabecular fibrils;
5 – polysomes at the
trabecular fibrillar
junction;
6 – microfilaments;
7 – cell membrane
Task 14. SCHEME OF TRANSPORT Task 15. SCHEME OF MICROVILLI AND CILIA
MOLECULAR STRUCTURE
Page 10 of 125
Ι Designate:
1– plasmalemma;
2– actin filaments;
3– villin;
4– calmodulin;
5– fimbrin;
6– fodrin;
7– cytoskeleton terminal
reticulum;
8 – intermediat filaments
ΙΙ
Designate: Ι - movement of molecules through the plasma membrane: a – Designate in the scheme: 3 – central membrane;
simple diffusion; b –carrier protein; c – channel protein; d – cotransport 4 – dineine arms
A –longitudinal B cross section
(simport); e – antiport. 5 – nexin linking protein;
1 – microtubule doublets (A and
6 – radial spoke ;
ΙΙ - vesicular transport: 1 phagocytosis; 2- pinocytosis: 3- receptor B
- basal corpuscle
mediatedtransport: a – cytoplasma;b – phagosoma; c – receptor; d - ligand 2 – central pair of microtubules;
Page 11 of 125
Groups of Types of contacts Space between Function
contacts two membrane c
Occluding Tight junction or - Non-permeable for
junctions zonula occludens micromolecules and
Cell –to - cell ions
Page 12 of 125
STAGES OF EMBRYONIC DEVELOPMENT, GAMETES,
Topic:
FERTILIZATION, CLEAVAGE, GASTRULATION Embryology – fundamental science about
developing organisms from fertilization
1. Basic periods of ontogenesis of vertebrates. until birth.
2. Germ cells. Morphological and functional characteristics. Role in transmitting genetic
information. Difference from somatic cells. Classification of oocytes.
3. Basic periods of embryogenesis of vertebrates. Main processes of embryonic development.
4. Fertilization. Definiton, essence, biological significance. DIFFERENCES BETWEEN GERM
5. Cleavage. Definiton. Interconnection between cleavage type and ovum structure.
Comparative characteristics of cleavage in different vertebrates. Types and structure of
CELLS (GAMETES) AND SOMATIC
blastulas. CELLS
6. Gastrulation. Definition. Types of gastrulation.
7. Organo- and histogenesis. Definition. Germ layers, their differentiation and derivatives. 1. Haploid nucleus
8. Extraembryonic organs of vertebrates, their functions. 2. Oocyte's NCR is 1 : 500
sperm's NCR is 1 : 0.5
somatic cells have NCR 1 : 6
3. Depressed metabolism
Definition of «Cleavage»: _____________________________________ 4. Necessity of protection and nutrition
____________________________________________________________________________________________________________________
______________________
5. High specialization.
_
Task 17. Task 18. SCHEME OF Task 19. Task 20. SCHEME OF
SPERMATOZOON STRUCTURE HUMAN SPERMATOZOON OOCYTE STRUCTURE OOCYTE STRUCTURE
Page 13 of 125
Preparation Spermatozoa of STRUCTURE Preparation Mammal ovum (under the electron
the guinea pig (under the electron microscope) Staining Hematoxylin- microscope)
Staining Iron hematoxylin eosin
Magnification 400Х Magnification 400Х
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Proliferation
Migration
Adhesion
on
Determination
Differentiation
Growth
Designate:
А – an example of primordial formation by cell migration;
Physiological death of cells B – an example of form formation by cellular layer modification:
1 – migration;
2 – local thickening;
3 – cellular strip;
4 – cellular layer fission;
5 – cellular layer curve (fold);
6 – bulging with an inner opening;
7 – bulging under the layer surface;
8 – cellular lamina;
9 – cellular trabecula;
10 – cellular nodule.
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Task 22 Comparative characteristic of vertebrate embryogenesis
Incraniate
Oocyte Amphibia Fish Birds Mammals
1.
2.
Cleavage
3.
4.
5.
Blastula
6.
Gastrulation
7.
Page 16 of 125
Neurula
Extraembryonic organs
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Topic: EARLY STAGES IF HUMAN EMBRYONIC DEVELOPMENT Definition of “Differentiation”___________________
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Task 23. FERTILIZATION
Designate:
А – contact interaction phase; B – sincarionic phase.
1 – chromosomes 6 – follicular cells of corona radiata
2 – oolemma 7 – tunic fertilization
3 – nuclear membrane
4 – polar bodies
5 – zona pellucida
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А B
Task 26. GASTRULATION
E - F:
А – delamination ( 8 days); 2 – hypoblast; 8 – notochord;
B, C, D – immigration (10-15 days); 3 – cytotrophoblast; 9 – mesoderm;
C – section along line I; D – section along line II; E 4 – syncytiotrophoblast; 10 – ectoderm;
(6 days.), F (18 days) – section along line III. 5 – primitive node; 11 – entoderm;
6 – primitive streak; 12 – neural groove;
Designate: 7 – prechordal plate; 13 – somites;
8 – notochord; 14 – coelom;
IN PICTURE А – D: 9 – formation of mesoderm 15 – parietal mesoderm layer
1 – epiblast; 16 – vesceral mesoderm
layer
C
D E F
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Task 27. SOMITES, NOTOCHORD, NERVE TUBE OF CHICKEN’S EMBRYO
Staining Hematoxylin
Magnification 80х, 400х
Colour:
Ectoderm – in green;
Endoderm – in red;
Mesoderm and its derivatives – in blue;
Notochord – in brown;
Nerve tube and ganglia – in yellow.
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29. CROSS-SECTION OF HUMAN EMBRYO
Task Task 30. DIFFERENTIATION OF EMBRYONIC LAYERS.
DURING THE 8TH -9TH WEEK OF Write the names of embryonic layers and their derivatives, learn the origin of
DEVELOPMENT. different tissue types.
Designate and colour:
1. Nerve tube (in yellow).
2. Spinal ganglia (in yellow) I. Nervous
3. Notochord (in brown)
4. Ectoderm (in green) Epithelial (stratified)
5. Somites: а-dermatome; b-myotome;
c-sclerotome (in blue)
6. Parietal mesodermal layer (in blue) II. Epithelial (simple cylindrical)
7. Visceral mesodermal layer (in blue)
8. Coelom 9. Mesenchyme
10. Endoderm (in red) III A Epithelial (mesothelium)
11. Primary vessels III.
III B Epithelial (simple cuboidal)
Page 22 of 125
Part IV. GENERAL HISTOLOGY
Topic:
EPITHELIAL TISSUES The basic types of tissues:
Page 23 of 125
Task 31. CHEME OF Task 32. SIMPLE SQUAMOUS SIMPLE CUBOIDAL
MOLECULAR
.
Task 33
EPITHELIUM EPITHELIUM OF
ORGANIZATION OF (MESOTHELIUM) RENAL TUBULES
BASAL MEMBRANE Staining Impregnation with
nitrogenous acidic silver- Hematoxylin-eosin
hematoxylin Staining 400X
Magnification 400X Magnification
А. А.
B.
B.
Designate:
Designate:
1– lamina lucida; А – cross-section of mesothelium: А – simple cuboidal epithelium;
2– lamina densa; 1 – basal membrane; Draw and designate:
3– reticular lamina; Draw and designate:
4– collagen fibrils; B – renal tubule:
B – portion of total preparation: 1 – cell borders;
5– laminin;
2– cell borders; 2 – nuclei;
6– proteoglycan; 3– cytoplasm;
7– type IY collagen. 3 – cytoplasm;
4– nuclei; 4 – basal membrane.
5– stomatas.
Page 24 of 125
Task 34. Task 35. Task 36.
STRUCTURE OF SIMPLE PSEUDOSTRATIFIED
ENTEROCYTE COLUMNAR CILIATED
INTESTINAL EPITHELIUM OF
EPITHELIUM TRACHEA
Staining Hematoxylin-eosin Staining Hematoxylin-eosin
X
Magnification 400 Magnification 400X
А.
Designate: Designate:
1– basal part of cell; B 1– ciliated cells;
2– apical part of cell; Draw intestinal epithelium and designate in 2– cilia;
3– nucleu scheme (А) and picture (B):
3– interca
4– microvilli; 1 – microvilli (border);
4– goblet cells;
5– secretory vesicles; 2 – columnar cells;
5 – basal membrane.
6– Golgi apparatus; 3 – basal membrane.
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Task 37. STRATIFIED SQUAMOUS Task 38. STRATIFIED SQUAMOUS Task 39. STRATIFIED
NONKERATINIZED EPITHELIUM OF KERATINIZED EPITHELIUM OF TRANSITIONAL EPITHELIUM OF
EYE CORNEA FINGER'S SKIN URINARY BLADDER
Staining: Hematoxylin-eosin Staining: Hematoxylin-eosin Staining: Hematoxylin-eosin
Magnification 400 X Magnification 80 X Magnification 400 X
Designate: Designate:
Designate:
1– basal membrane; 1– basal membrane; А – epithelium in the stretched state;
2– basal layer; 2– stratum basale; B – epithelium in the non-distended
3– intermediate layer; 3– stratum spinosum;
4– stratum granulosum; state:
4– flattened surface layer;
5– germinal layer. 5– stratum lucidum; 1– basal membrane;
6– stratum corneum; 2– basal cells;
7 – granules in the cells of stratum 3– intemediate cells;
granulosum. 4– surface cells.
Page 26 of 125
Task 40. GIVE FULL NAMES OF THE Task 41. CALL MORPHOLOGICAL TYPES Task 42. DESIGNATE THE TYPE OF
EXOCRINE GLANDS OF THE ENDOCRINE GLANDS SECRETION
1. 5.
1 – Merocrine
2. 6.
2 – Apocrine
3. 7.
4. 8. 3 – Holocrine
B
Correct tasks №№___________________
Designate:
1 – secretory cells, Teacher
2 - capillaries Date «____» _____________ ______ .
Page 27 of 125
Topic:
BLOOD AND LYMPH
HEMATOGRAMMES
1. Blood and lymph as types of inner tissues.
2. Functions of blood and lymph.
3. General morphology of blood. Corpuscular elements of blood, their Findings Value
classification.
4. Erythrocytes: shape, size, structural peculiarities, function, duration of
life.
5. Leucocytes: classification, shape, size, structure, function, duration of Hematocrit 0,35-0,55
life. Hemaglobin 120-140 g/l
6.Thrombocytes: shape, size, structure, function, duration of life. Erythrocytes F - 3,9-4,9х1012 /l
7. Hemogramme. Hematocrit. Leucocytic formula, shift of blood formular М - 4,7-5,2х1012 /l
to the left. Reticulocytes 0,6-0,8%
Leucocytes 3,8-9,8х109 /l
Thrombocytes 200-400x109 /l
ESR 3-8 mm/h
12. Functions of blood:
1. _________________________________________________________ Leucocytic formula (%)
2. _________________________________________________________
3. _________________________________________________________ Neutrophils Altogether 65-70
Among them: Y-ng. band-n. Segm-n
4. _________________________________________________________ 0-1 1-4 60-70
5. _________________________________________________________
Eosinophils 1-5
6. _________________________________________________________ Basophils 0-1
Lymphocytes 20-40
Monocytes 6-8
Page 28 of 125
Task 43.
Staining CELLS OF HUMAN BLOOD
Magnification Hematoxylin-eosin
400Х
MEMBRANE AND CYTOSKELETON OF
Blood cells Size, functions ERYTHROCYTE
Designate:
Erythrocyte 1 – Basic transmembraneous protein; 2 – spectrin; 3 –
actin; 4 – stabilizing protein; 5 – ankyrin.
Page 29 of 125
Lymphocyte Lymphocyte in smear Size, function
(agranulocyte Designate:
1 – nucleus;
2 – lysosomes;
3 – ribosomes;
microvilli.
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Neutrophil Designate: Neutrophils in smear: Size, function
1 – nucleus; a- young; b - band; c -
2 – specific granules; segmented
3 – azurophilic granules;
4 – glycogen;
5 – pseudopodia.
Spesific granules
contain:
1.
2.
3.
Spesific granules
contain:
1.
2.
3.
4. Correct tasks №№___________________
5.
Teacher
Date «____» _____________ ______ .
Page 31 of 125
Task 45 SCHEME of HAEMOPOIESIS
Page 32 of 125
TASK 46 SCHEME OF ERYTHROPOIESIS TASK 47 SCHEME OF GRANULOPOESIS
5.
6.
Page 33 of 125
Task 48/ SCHEME OF LYMPHOPOIESIS Ι - antigen independent differentation
ΙΙ - antigen dependent differentation
АG – antigens
Prе–Т – precursory Т-lymphocyte cells
Ι Prе–В – precursory В-lymphocyte cells
CLО – central lymphoid organs
Ι PLО – peripheral lymphoid organs
Designate:
Pre–Т Pre–В
CL 1 – stem hemapoietic cell;
2 – hemi-stem hemapoietic cell;
3 – antigens;
4 – macrophage;
BLOOD
5 – Т–lymphocytes;
6 – В– lymphocytes;
AG
7 – memory cells;
ΙΙ 8 – effector Т-lymphocytes;
9 – plasmatic cells;
10 - banned clones of lymphocytes
PLO
Page 34 of 125
Topic: FORMATION OF blood cells (HEMoPOIESIS)
13. 1. Modern theory of hemapoiesis. Stem hemapoietic cell. Task 44. DEVELOPMENT STAGES OF HEMOPOIESIS IN
Differones and hemopoietines. HUMAN BEINGS
14. 2. Embryonic hemapoiesis in the york sac, liver, thymus, 21.
spleen, lymph nodes, red bone marrow.
15. 3. Erythropoiesis.
16. 4. Granulopoiesis.
17. 5. Monocytopoiesis. Mononuclear phagocytic system.
18. 6. Lymphopoiesis (antigen independent) in central lymphoid
organs. Т- and В-lymphocyte systems. Cellular and humoral
immunity.
19. 7. Lymphopoiesis in peripheric lymphoid organs, its
dependence on antigen activity.
20. 8. Cell cooperative interactions in immune response. 22.
Age of the fetus (in terms of months)
CENTRAL HEMOPOIETIC ORGANS
Corpuscular elements formed Color different periods of hemopoiesis:
25. Name of organ
In embryonic period After burth mesoblastic period – in yellow;
26. Red bone marrow 29. 30. hepatic period – in green;
27. 23. myeloid period – in red.
28. Thymus 24.
PERIPHERY HEMAPOIETIC ORGANS
Corpuscular elements formed
Name of organ
In embryonic heriod After burth
Spleen
Lymph nodes
Tonsils
Appendix
Solitary nodules
Page 35 of 125
Task 45 SCHEME of HAEMOPOIESIS
Page 36 of 125
TASK 46 SCHEME OF ERYTHROPOIESIS TASK 47 SCHEME OF GRANULOPOESIS
5.
6.
Page 37 of 125
Task 48/ SCHEME OF LYMPHOPOIESIS Ι - antigen independent differentation
ΙΙ - antigen dependent differentation
АG – antigens
Prе–Т – precursory Т-lymphocyte cells
Ι Prе–В – precursory В-lymphocyte cells
CLО – central lymphoid organs
Ι PLО – peripheral lymphoid organs
Designate:
Pre–Т Pre–В
CL 10 – stem hemapoietic cell;
11 – hemi-stem hemapoietic cell;
12 – antigens;
BLOOD 13 – macrophage;
14 – Т–lymphocytes;
AG
15 – В– lymphocytes;
16 – memory cells;
ΙΙ 17 – effector Т-lymphocytes;
18 – plasmatic cells;
Page 38 of 125
Topic: CONNECTIVE TISSUES
Page 39 of 125
Mesenchyma (origin, localization, morphological and functional characteristics), Task 49. ORGANIZATION OF CONNECTIVE
its derivatives. TISSUE.
1. General characteristics and classification of connective tissues.
2. Development, structure and functions of loose connective tissue.
3. Characteristics of loose сonnective tissue cells. Cells:
4. Formation and structure of intercellular substance loose connective tissue . 1.
5. System of mononuclear phagocytes.
6. Specialized connective tissue. 2.
7. Structure and functions of dense fibrous connective tissues. 3.
31.
32. Classification of connective tissues 4.
5.
6.
7.
8.
9.
10.
INTRACELLULAR SUBSTANCE
Fibers:
1.
2.
3.
Ground amorphous substance
1.
2.
Page 40 of 125 3.
33. Task 50. 34. Task 51. LOOSE IRREGULAR 35. Task 52. STRUCTURAL ORGANIZATION OF
MESENCHYMA IN CONNECTIVE TISSUE COLLAGENOUS SUBSTANCE AND ELASTIC FIBERS
EMBRYONAL
SECTION
36. Stain 39. Hematoxyl 41. Stainin 43. Ferrous hematoxylin 45. Aminoacids are taken up by
ing in-eosin g 44. 400Х cell and linked to form
37. 40. 400Х 42. Magni 46.
38. Mag f. 47.
nif. 48. PRO-α- CHAINS
76. 91. 49.
77. 92. 50.
78. 93. 51. α- CHAINS
79. 94. 52.
80. 95. 53.
81. 96. 54. Three such chains join to form a
82. 97. 55.
83. 98. 56.
84. 57.
85. 58. PROCOLLAGEN
86. MOLECULE 73.
87. 59.
88.
89.
90. 60.
Draw and designate: 61. Such molecules leave the cell
through secretory vacuoles to form
62.
63.
64. TROPOCOLLAGEN
MOLECULES
65.
66.
67. 74.
75.
А Б
Page 41 of 125
1 – mesenchymal cells Draw and designate: 68. Which aggregate to form Designate:А
nuclei; 69.
1– collagenous substance; 70. 100. 1. polypeptide chain;
2 – mesenchymal cells
2– elastic fiber; 71. 101. 2. tropocollagen;
processes;
3– basic substance; 72. COLLAGEN FIBERS 102. 3. protofibrils
3 – intercellular
4– fibroblast; 103. 4. striated fibril;
substance;
5– macrophage; 104. 5. collagenous substance;
4 – blood vessel.
6– lymphocyte. 105. 6. spinal structure of
7– mast cell nucleus; collagen micromolecule
8– granules in mast cell cytoplasm; 106. B
9– mast cell degranulation 107. 6. elastin;
99. 108. 7. microfibrils
109. 111.
110. Task 53. 112. ULTRASTRUCTURE OF LOOSE IRREGULAR CONNECTIVE TISSUE CELLS
Designate:
115.I – fibroblast:
1 – granular endoplasmatic reticulum;
2 – complex Golgi;
3 – collagen and elastic fiber.
Name:
116. precursory cell _____________________
117. cellular
function_______________________
118.I I – macrophage (histiocyte):
1 – lisosome;
2 – phagosome;
113. 3 – pinocytotic vesicles.
114.
Page 42 of 125
Name:
119. precursory cell_____________________
120. cellular function
_______________________
121.III – plasmocyte:
1 – ergastoplasm;
2 – complex Golgi .
Name:
122. precursory cell_____________________
123. cellular function
_______________________
124.IV – Mast cell (labrocyte):
1 – heterogenous granules with heparine and
histamine.
127. Name:
128.
125. precursory cell_____________________
126.cellular function
_______________________
129. 134. 136.
130.Task 54. ADIPOSE TISSUE 135. Task 55. WHITE 137.Task 56. DENSE REGULALY
131. ADIPOSE TISSUE CONNECTIVE TISSUE OF THE
132. TENDON
138. Staini 140. Sudan 142. Staining 144. Hematoxylin-eosine
ng III 143. Magnif. 145. 400Х
139. Magn 141. 400Х
if.
Page 43 of 125
146.
Page 44 of 125
147.
148.
149.
Draw 3-4 cells and 150.
151.
designate: 152.
1 - adipocyte nucleus; 153.
154.
2 - fat droplet. 155. А – cross-section
156. B – longitudinal section
Draw and designate in the picture and
scheme:
157.1 – fibrocytes; 2 – 1st level bands; 3 –
2nd level bands; 4 – peritenoneum; 5 –
endotenoneum; 6 – blood vessels.
Designate:
A- white adipose tissue; B – brown adipose tissue
1 – adipocyte;
2 – adipocyte nucleus;
3 – fat droplets;
4 – mitochondria;
5 – blood capillaries;
6 – nerve fiber.
Page 45 of 125
Topic: CARTILAGINOUS AND bone TISSUES
158. General morphological and functional characteristics, Task 57. CLASSIFICATION OF SKELETAL
classification of cartilaginous tissues. TISSUE
159. Structural peculiarities of different cartilaginous tissue types.
160. Development, growth, regeneration and age-related changes of
cartilage as an organ.
161. General morphological and functional characteristics and
classification of bone tissues. Bone as an organ. Periosteal
structure and function.
162. Structure of reticulofibrous (woren. immature) bone tissue:
cells, intercellular substance.
163. Structure of lamellar (mature) bone tissue. Compact and
spongy (cancellous) bone substance.
164. Development of bone tissue (direct and indirect osteogenesis).
Page 46 of 125
Task 58. HYALINE CARTILAGE Task 59. ELASTIC CARTILAGE Task 60. FIBROUS CARTILAGE
Hematoxylin-eosin Hematoxylin-eosin Staining Hematoxylin-eosin
Staining 400Х Staining 400Х Magnification 400Х
Magnification Magnification
Page 47 of 125
Task 61. INTRAMEMBRANOUS Task 62. ENDOCHONDRAL OSSIFICATION (INDIRECT
OSSIFICATION OSTEGENES)
Staining (DIRECT Staining Hematoxylin-eosin
Magnific. OSTEOGENES) Magnific. 400Х
Hematoxylin-eosin
400Х
ΙΙΙa Designate;
ΙΙ Ι Hyaline cartilage “model”;
Ι
ΙΙ primary ossification center
1.bony collar –perichondral bone
ΙΙΙ calcified cartilage
a osteogenic cells,osteoclasts,
blood vessels penetrate into
cartilage;
b forming enchondral bone
2. zone of resting cartilage;
3. zone of proliferating
cartilage (column cartilaginous
Draw and designate: cells);
4.. zone hypertrophied cartilage
1– mesenchyma; 5. zone calcified cartilage;
2– osteoblasts; 6.zone of resorption and
3– osteocytes; ossification;
4– osteoclasts;
5– intercellular substance of
reticulofibrous bone tissue;
6 – blood vessels.
ΙΙΙb
Page 48 of 125
Task 63. STRUCTURAL SCHEME OF COMPACT Task 64. CROSS-SECTION OF COMPACT
SUBSTANCE of LONG BONE Staining SUBSTANCE of LONG BONE
Magnification After Schmorl
400Х
Designate:
1 – osteon canal;
2 – osteon lamellae;
3 – lacuna (osteocyte bodies);
Designate:
1– periosteum with blood vessels; 4 – canaliculi osseum (osteocyte processes);
2– blood vessels; 5 - interstitial lamellae
3– outer layer of general lamellae ;
4– osteons;
5– osteon canals; Correct tasks №№___________________
6– trophic canal; Teacher
7– system of interstitial lamellae; Date «____» _____________ ______ .
8– inner layer of general lamellae;
9– endosteum.
Page 49 of 125
Topic: MUSCULAR TISSUES
8. General characteristics of muscle tissues (classification, sources of 165. Muscle tissues provide movement of the whole body,
development, location in the body, basic structural principles, functions, its parts and inner organs.
regeneration, peculiarities of blood supply and innervation). 166. It results from constriction (shortening) of muscle cells
9. Comparative characteristics of different types of muscle tissue structural units: or fibers as a result of contraction of their special elements
morphofunctional units, peculiarities of its structure, principles of location in – myofilaments in the form of actin and myosin.
the tissue and types of intercellular contacts. 167.
168.
10. Smooth muscle tissues: structure of smooth myocytes, contraction apparatus 169. Task 65. MAIN CHARACTERISTICS OF
and molecular mechanism of contraction. 170. VARIOUS TYPES OF TISSUES
11. Skeletal muscle tissue. General plan structure of the muscle fibres. Contraction 171. 172. Skeleta 175. Cardia 178. Smooth
apparatus of muscle fibres. Structure of sarcomere. Mechanism of muscle l c 179. muscula
contraction. 173. muscle 176. muscle r
174. tissue 177. tissue 180. tissue
12. Structure of skeletal muscle as an organ. Myon. Comparative characteristic of 181.
different types of skeletal muscular fibers (red, white and intermediate fibers). 182. Sources of 184. 185. 186.
13. Cardiac muscular tissue. Comparative characteristic of different types of 183. developme
cardiomyocytes (contractile and conductive cardiomyocytes). nt
187. Structural 189. 190. 191.
188. and
functional
unit
192. Number of 194. 195. 196.
nuclei and
193. its
location
197. Capability 200. 201. 202.
of
198. regenerati
on
199.
203.
Page 50 of 125
204. 206. 208. 211.
205. Task 66. 207. SCHEME OF SMOOTH MUSCLE CELL′S ORGANIZAION 209. Task 67. 212. SMOOTH MUSCULAR TISSUE
OF URINARY BLADDER WALL
Staining 213. Hematoxylin-eosin
210. Magnifica 214. 400Х
tion
220. A
221.
222. B
215.
216.
217.
218.
219.
Designate:
223. 1 - nucleus; 2 – plasma membrane; 3 – caveolae ; 4 – mitochondria; 5 – smooth 224.
endoplasmic reticulum; 6 – intermediate filaments; 7 – thin (actin) filaments; 8 – 225. Designate scheme (A) and picture (B):
thick (myosin) filaments; 9 – dense bodies; 10 – gap junction between cells. 226.
1 – muscular cells in longitudinal section
2 – muscular cells in cross section
3 – muscular cells nuclei
4 – connective tissue (perimysium)
Page 51 of 125
SKELETAL MUSCLE SCHEME OF MUSCLE'S
Task 68. TISSUE OF THE Task 69. STRUCTURE AS AN Task 70. CARDIAC MUSCLE
TONGUE ORGAN TISSUE
Staining Iron hematoxylin Staining Iron hematoxylin
Magnification 400Х Magnification 400Х
A
B
B
Designate:
Designate:
227. А – structure of muscle: 1- cardiomyocyte nucleus;
228.Designate scheme (A) and picture (B): 1 – epimysium; 2 – perimysium; 3 – 2- intercalated disc;
229. muscular fiber. 3- anastomosis;
230.1 – longitudinal section of muscle B – structure of myon: 4- myofibrils;
fibers; 2 – transversal section of 4 - muscle fiber (myosymplast); 5 – 5- connective tissue.
muscle fibers; 3 - sarcolemma; 4 – myosatellitocyte; 6 – α-motoneuron axon and
musculаr fiber nucleus; 5 – its contact with myosymplast; 7 –
endomysium; 6 – perimysium. endomysium: 7а – capillaries; 7b – elastic
and reticular fibers.
Page 52 of 125
Task 71. Stuctural Scheme Of Muscular Tissue Elements
Designate:
231.А – smooth muscular tissue myocyte
(SMT);
А 232.B – skeletal muscular tissue
myosymplast;
233.C – contractile cardiomyocyte;
234.D – conductive cardiomyocyte.
Supporting structure
1 – sarcolemma: 1а – cytolemma; 1b – basal
membrane; 1c – Т-tubules; 2 – myofibril
attachment site: 2а – in SМT (dense bodies and
B dense stripes); 2b – in myosymplast – Z– and
M–lines;
Trophic apparatus
3 – nucleus; 4 – mitochondria; 5 – smooth
endoplasmatic reticulum (Са++ depot): 5а –
SMT vesicles and caveolae; 5b – L-tubules and
terminal cisterns in myosymplast; 6 –
ribosomes; 7 – glycogen inclusions; 8 –
myoglobin inclusions;
C Contractile apparatus
9 – actin and 10 – myosin myofilaments;
Intercellular contacts
11 – nexuses; 12 – desmosomes; 13 –
interdigitations;
Cross sections
D
14 – nuclei; 15 – myofibrillar fields.
Page 53 of 125
Task 73. COMPARATIVE
Task 72. CONTRACTION OF STRIATED AND SMOOTH MUSCULAR
CHARACTERISTICS OF VARIOUS TYPES
TISSUES
OF CARDIOMYOCYTES
Contractile Conductive
А
Functions
B
Type of exchange
C Resistance to
oxygen
Relaxation Contraction insufficiency
Predominant type
of inclusions
Designate:
Mitochondria
А – sarcomere contraction: 1- band I; 2 – band A; 3 – Z–line; 4 - M–line; 5 – H–zone; 6 – (number and size)
sarcomere.
Myofibrils
B – myofibrillar contraction: 7 – M–line; 8 – H–zone; 9 – myosin filaments; 10 – actin
(number and
filaments.
location)
C –SMC contraction: 11 – actin myofilaments; 12 – myosin myofilaments; 13 – sites of
Location in
actin myofilament attachment in cytoplasm (dense bodies) and 14 – to cell carcolemma
myocardium
(dense stripes)
Page 54 of 125
Topic: NERVE Tissue
235.
1. Nerve tissue. General morphological and functional characteristics. Classifications of neurons:
Sources of development, histogenesis.
2. Neurons. Classification (morphological, functional, mediatorial).
Morphological and functional characteristics of neurons. Transporting I. Morphological
processes in neurocytes.
3. Neuroglia. Classification and development. Morphological and
functional characteristics of different types of neuroglia. 1.
4. Nerve fibers. Classification. Morphological and functional 2.
characteristics of myelinated and unmyelinated fibers. Process of
3.
myelinisation. Degeneration and regeneration of nerve fibers.
4.
5. Nerve endings (receptive and efferent types), classification and
structure.
6. Interneuronal synapses. General plan of synapses organization. II. Functional
Classification, structure, transmission of nerve impulse.
236. 7. Reflex arch - morphological base of nervous activity. Basic structural elements
of a reflex arch. Simple and complex reflex arches. 1.
237.
2.
3.
I – 4.
Development sources of nerve tissue II –
III –
Page 55 of 125
238. Morphological and functional unit of nerve tissue: ________________ III. Mediatorial
239. ___________________________________________________________
1.
2.
3.
4.
5.
240.
Task 74. STRUCTURAL SCHEME OF
THE NEURON
Mark:
1 – nerve cell nucleus;
2 – axon;
3 – dendrites;
4 – cell body;
5 – basophilic substance (Nissl bodies);
6 – axon hillock;
7 - oligodendrocyte;
8 - recurrent axonal branch;
9 - axodendritic synapse;
10 - axial cylinder;
11 - myelin sheath; 241.
12 - node of Ranvier; 242. 246.
13 - Schmidt-Lanterman cleft; 243. Task 75. BASOPHILIC SUBSTANCE OF 247.Task 76. NEUROFIBRILS OF
14 - lemmocyte nucleus; THE SPINAL CORD'S NEURONS THE SPINAL CORD'S NEURONS
15 - neuromuscular synapse; 244. Staining: Toluidine blue 248. Staining: Silver impregnation
16 - striated muscular fiber. 245. Magnification 400Х 249. Magnification 400Х
250.
Page 56 of 125
251.
252.
253.
Designate:
1 – nucleus; Designate:
2 – basophilic substance (Nissl bodies); 1– nucleus of neuron;
3 – axon; 2– neurofibrils;
4 – dendrites. 3– axon;
4– dendrites.
Task 77. MICROGLIA Task 78. SCHEME OF INTERACTIONS Task 79. SCHEME OF HEMATO-LIQUER
Staining: Silver ASTROCYTE WITH HEMOCAPILLARY BARRIER
Magnification impregnation AND NEURON
400Х
Page 57 of 125
Draw and designate: Deignate:
Page 58 of 125
Task 80. NON-MYELINATED NERVE FIBRES
Staining Hematoxylin-eosin
Magnification 400Х
A
Designate picture (A) and scheme (B):
1 – lemmocyte nuclei; 3 – axial cylinders;
2 – non-myelinated nerve fibres; 4 - mesakson.
254.
255. B
A 260. B
Designate picture (A) and scheme (B):
9– 10 – 262.
Page 59 of 125
Task 82. EFFERENT ENDING OF Task 83. NEUROMUSCULAR SYNAPSE Task 84. NONFREE
SMOOTH MUSCLE 263. NONENCAPSULATED ENDINGS
264. 265.
266. 268.
Designate:
267.Designate: 269. Designate:
1– smooth cells; 1– presynaptic membrane;
2– axon; 2– synaptic vesicles;
270.
271. 1 – terminal of sensory nerve fibres; 2 –
3– axon terminals; 3– axon mitochondria;
modified Schwann cells; 3 – nucleus of
4– varicose dilations; 4– oligodendrocyte;
Schwann cell; 4 – basal lamina.
5– vesicles with mediator; 5– postsynaptic membrane;
272.
6– mitochondria. 6– myosymplast mucleus;
7– mitochondrion of muscle fiber;
8– myofibrils;
9– synaptic cleft.
Page 61 of 125
Task 110. ULTRAMICROSCOPIC STRUCTURE OF THE Task 111. ULTRAMICROSCOPIC STRUCTURE OF THE
LYMPHATIC CAPILLARY WALL BLOOD-CAPILLARY WALL
Designate:
278. А — somatic capillary;
B — fenestrated capillary;
C — sinusoidal capillary.
279.
1 — endothelial cells (EC);
2 — EC nucleus; 3— basal
membrane; 4 — pericyte; 5
— EC fenestras; 6 — pores in
the EC.
280. The Task 112. SMALL BLOOD VESSELS OF THE SOFT BRAIN
MEMBRANE
281. Designate:
1. Arteriole.
2. Venule.
3. Capillary.
4. Myocytes.
Designate:.
5. Endotheliocytic nucleui.
277. 6. Adventitious cells.
1 — endothelial cells (EC); 2 —EC nucleus; 3 — discontinuous 7. Erythrocytes.
basal membrane; 4 — anchoring (collagen) fibrils. 8. Connective tissue cells.
282.
Page 62 of 125
Task 113 MUSCULAR Task 114 ELASTIC ARTERY (aorta) Task 115. VEIN
ARTERY Staining Orsein Staining (femoral vein)
Х
Staining Haematoxylin-eosin Magnification 400 Magnification Haematoxylin-eosin
Magnification 400Х 400Х
Draw and to designate: Draw the preparation and designate the Draw and designate:
I – internal membrane: elastic fibres and window I – internal membrane:
1 – endothelium; membranes(fenestras) of: 1 – endothelium;
2 – subendothelial layer; 2 – subendothelial layer;
3 – internal elastic membrane; 1 – internal membrane; II – medial membrane:
II – the medial membrane: 2 – medial membrane; 3 – muscular cells;
4 – muscular cells; 3 – external membrane. 4 – elastic fibres;
5 – elastic fibres;
III – external membrane:
6 – external elastic membrane;
5– collagenous fibres;
III – external membrane: 6– connective tissue cell nuclei;
7 – collagenous fibres;
8 – connective tissue cell nuclei;
7– elastic fibres;
9 – elastic fibres; 8– vessels of the vessels.
10 – vascular vessels.
Page 63 of 125
Task 116. SCHEME OF HEART DEVELOPMENT Task 117. THE CARDIAC WALL
Staining Haematoxylin-eosin
Magnification 400Х
283.
Draw and to designate:
I. Endocardium
1. Endothelial layer.
2. Subendothelial layer.
3. Elastic muscular layer.
4. External connective tissue
membrane.
II. Myocardium
5. Nuclei of contractile
cardiomyocytes.
6. Fixed intercalated discs.
7. Connective tissue with
capillaries.
8. Conductive
cardiomyocytes (Purquinier’s
fibres).
9. Anastomoses between
cardiomyocytes.
To designate: III. Epicardium
10. Subserous basis .
А — paired rudiments of the heart ; B, C, D — their
11. Proper lamina of serous
approchement; E — junction into one unpaired rudiment. membrane.
1 – ectoderm; 2 – entoderm; 3 – parietal mesodermal layer; 4 – 12. Mesothelium.
visceral mesodermal layer; 5 – nerve groove; 6 – nerve tube; 7 –
celome; 8 – ventral aortas; 9 – dorsal aortas; 10 – anterior
13. Correct tasks №№___________________
intestine; 11 – junction between ventral aortas; 12 –
14. The lesson is completed
endocardium; 13 –myocardial and epicardial rudiments; 14 –
pericardial cavity. 15. Lecturer _____________
Date _________________
Page 64 of 125
UNIT ΙV. PARTICULAR HISTOLOGY
Topic NERVOUS SYSTEM.Peripheral nervous system
1. Definition of organ. Classification of organs. Definition of organ____________________________
2. General morphological and functional characteristics of the
____________________________________________
nervous system. Sources of development. Classification. ____________________________________________
3. Neuronal theory .Conception of nerve centers, their
classification, principles of structural organization.
Classification of organ
4. Reflex arch, its structural unit. Types of reflex arches.
5. Cerebrospinal ganglia: sources of development, structure, Type organ Character of organ
function
6. Spinal cord. Its development. The structure of grey and
white matter. Neuron structure.
7. Peripheral nervous system. Nerve, its structure and
regeneration
8. Autonomous (vegetative) nervous system: extra- and
intramural ganglia.
Stroma_____________________________________
____________________________________________
Parenchyma_________________________________
____________________________________________
Page 65 of 125
Task 85. NEURON INTEGRATION
Page 66 of 125
Task 86. REFLEX ARCHES
Page 67 of 125
Task 87. CEREBROSPINAL Task 88. PERIPHERAL NERVE Task 89. VEGETATIVE NERVOUS SYSTEM
GANGLION GANGLION
Staining Hematoxylin-eosin
Staining X Staining Impregnation with silver
Magnification
Hematoxylin-eosin Magnification 400 Magnification 400Х
400X
_
Designate
Correct tasks No______________________ 1 – long-axon nerve cell (Dogel ) type
The tasks are completed 2 – equal length of processes nerve cell (Dogel 2)
Lecturer________________________
3 – nerve fibers
Date ___________________________ 4 – nuclei of glial cells
Page 68 of 125
Topic NErVOUS SYSTEM. CENTRAL NERVOUS SYSTEM
Task 90. TYPE OF CORTEX CEREBRAL
9. Embryonal development of the brain HEMISPHERES
10. Cerebellum. Structure and functional characteristics.
Neuronic ensembles of the cerebellar cortex as a screening
center.
11. Brain. Cerebral hemispheres, sources of embryonic
development. Histogenesis. Neuronic organization of the
cerebral hemispherical cortex. Cyto- and
myeloarchitechtonics. Conception of modules and columns.
Localization of functions in the cortical centers
12. Brainstem. Sources of development. Grey and white matter.
Organization principles of ascending and descending
passageways. Medulla, nuclei. Reticular formation: structure
and functions
Characteristic of agranular
cortex
Characteristic of granular
cortex
Correct tasks No______________________
The tasks are completed
Lecturer________________________
Date ___________________________
Page 69 of 125
Task 91. CORTEX OF CEREBELLAR Task 92. SCHEMATIC DRAWING of BRAIN
Staining HEMISPHERES CORTEX COLUMN (MODULE)
Impregnation with silver
Magnification
80Х, 400Х
Input of
information
Zone of Inhibition neurons
information
treatment
Exiting neurons
Exit of
analyzed
information
Draw and designate:
1 – molecular layer; Draw and designate:
2 – external granular layer;
3 – pyramide layer; 1 – stellate neuron ; 2 – pyramidal neuron; 3 afferent fibers (green); 4 – efferent pathway
4 – internal granular layer; (red)
5 – ganglionic layer;
6 – polymorphic cellular layer;
7 -- white matter of the hemispheres
Page 70 of 125
Task 93. CROSS SECTION OF Task 94. CEREBELLAR Task 95. INTERACTION BETWEEN
THE CEREBRAL GYRUS GLOMERULUS NEURONS AND AFFERENT FIBRES IN
Staining Impregnation with silver NEURON ENSEMBLES OF CEREBELLAR
Magnification. 400X CORTEX (after J. Eckls)
Page 71 of 125
Topic: SENSE ORGANS
1. Sense organs. General morphological and functional characteristics. 290.
Conception of analyzer, its structure. 291. The main functional apparatuses of the
2. Classification, structure and cytophysiology of receptor cells.
3. The organ of smell: structure, sources of development, cytophysiology of eye:
neurosensory receptor cells. 292.
4. The organ of vision. Sources of development and basic stages of 293. 1 – refractive apparatus
embryogenesis. 294.
5. The main functional apparatuses of the eye. Structure of the refractive and 295. includes:
accommodative apparatuses of the eye ball. Their age-specific changes. 296.
6. The receptive apparatus: development, structure, and adaptation of the 297.
retina to the level of light. Specialized regions of the retina. Types of 298.
neurosensory receptor cells. 299.
7. The organ of a taste: source of development, structure, cytophysiology of 300. 2 – accommodative apparatus
taste receptor cells. 301.
8. The organ of hearing. Morphological and functional characteristics. 302. includes:
Sources of development, structure, cytophysiology of sensoepithelial 303.
receptor cells of the spiral organ. 304.
9. The organ of equilibrium. Sources of development, structure, functions. 305.
Morphological and functional characteristic of sensoepithelial receptor 306.
cells.
284. 307. 3 – receptive apparatus
285. 308.
286. Definition of the “Sense organ”: 309. includes:
287.
288.
289.
Page 72 of 125
310. Task 96. RECEPTOR CELLS Task 97. CORNEA Task 98. POSTERIOR WALL OF EYE
Staining Hematoxylin-eosin Staining Hematoxylin-eosin
Magnification 80Х, 400Х Magnification 80Х, 400Х
311.
312.
Designate neurosensory (1) and Designate: Draw eye wall sections (in the dark ness and in the
sensoepithelial (2) cells. Indicate 1 – nonkeratinized stratified light) and designate in the picture and in the diagram:
their location: squamous epithelium;
I – choroid:
2 – anterior basement membrane 3 – blood vessels; 4 – pigmentary tissue;
1. 1 – (Bowman's membrane); II – retina:
2– 3 – substantia propria of the cornea; 5 – pigment epithelium; 6 – layer of rods and cones; 7 –
2. 1 – 4 – posterior basement membrane external limiting membrane; 8 – outer nuclear layer; 9 – outer
(Descemet's membrane); plexiform layer; 10 – inner nuclear layer; 11 – inner plexiform
2– 5 – posterior epithelium of the cornea layer; 12 – layer of ganglion cells; 13 – nerve fiber layer; 14 –
3– (endothelium). internal limiting membrane.
Page 73 of 125
Task 99. Task 100. Task 101.
ULTRASTRUCTURE OF ROD AND CONE OLFACTORY AREA OF THE NASAL SCHEME OF A TASTE BUD
CAVITY
320.
321. Designate:
322. Designate:
323. 331. 1 – taste pore;
313. 324. 1 – olfactory cell; 332. 2 – microvilli;
314. Designate: 325. 2 – apexes with cilia; 333. 3 – taste cell;
315. I – rod II - cone 326. 3 – supporting cells; 334. 4 – supporting cell;
316. 1 – outer segments; 6 – cilium; 327. 4 – microvilli; 335. 5 – basal cell;
317. 2 – inner segment; 7 – synapses with 328. 5 – basal cells. 336. 6 – afferent nerve fiber.
318. 3 – mitochondria; bipolar cells. 329.
319. 4 - nuclei; 330.
Page 74 of 125
Task 102. SCHEME OF A MACULA Task 103. SPIRAL ORGAN
WITHIN THE UTRICLE Staining Hematoxilin-eosin
Magnification 400Х
337. 339.
340.
341.
338.
Designate: Designate:
1– type I hair (sensory) cell; 1 – outer hair cells; 11 – stria vascularis epithelium;
2– type II hair cell; 2 – inner hair cells; 12 – capillaries;
3– supporting epithelial cells; 3 – outer supporting cells; 13 – spiral ligament.
4– kinocilium; 4 – inner supporting cells; 342.
5– nerve endings; 5 – outer pillar cell;
6 – inner pillar cell;
6– otolithic membrane;
7 – otoconia; 7 – tunnel;
8 – endolymph. 8 – tectorial membrane; 16. Correct tasks №№___________________
9 – basilar membrane; 17. Teacher
10 – vestibular membrane; Date «____» _____________ ______ .
Page 75 of 125
Topic integumentary system (skin)
Page 76 of 125
Task 105. SKIN OF THE FINGER Task 106. TYPES OF SKIN EPIDERMIS CELLS
Staining: Hematoxylin-eozin
Magnification: 400 х
Designate:
I – keratinocytes:
1 – basal cell;
II – melanocyte:
2 – spinous cell;
5 – melanosomes of melanocytes;
3 – granular cell;
4 – corneocyte. III – dendrocytes:
6 – tennis racquet-shaped granules.
Page 77 of 125
Task 108. SKIN AND ITS APPENDAGES (SCHEME) Task 109. FORMATION OF MELANIN
PIGMENT AND SECRETION IT
INTO KERATONOCYTES
Designate:
1 – epidermis;
2 – dermis;
3 – cortex of the hair;
4 – medulla of the hair;
5 – hair cuticle;
6 – internal root sheath;
7 – external root sheath;
8 – connective tissue sheath;
9 – hair bulb;
10 – hair papillae;
11 – sebaceous gland;
12 – arrector pili muscle;
13 – eccrine sweat gland;
14 – apocrine sweat gland,
15 – blood vessels. Designater:
1- keratinocytes,
2- melanocytes
Page 78 of 125
Topic
Digestive system: organs of THE oral cavity, oesophagus
Digestive system: development sources and their derivatives, general Organs of oral cavity:
structure (membranes and layers), functions.
Oral cavity, structural organization of its mucous membrane and function. 1.
The lip, gum, cheek, hard and soft palate. Their histological and 2.
physiological features. 3.
Structure of the tongue. Characteristics of tongue papillae. Taste buds. 4.
Teeth. Sources and basic stages of development. Microscopic structure of 5.
dentine, enamel, cementum and pulp. Tooth supporting structures. 6.
Age-related changes of the oral mucous membrane. 7.
Oesophagus. Sources and features of development. Oesophageal
membrane structure and their topographic features. Functions of the The structural peculiarities of the oral cavity
oesophagus. mucosa:
Epithelium-
- is stratified squamous keratinized (gingiva,
hard palate) or nonkeratinized (soft palate, lips,
cheeks, floor of the mouth)
- has a large thickness (200-600 μm)
- has a high capability of regeneration
Call the main development stages of teeth: Lamina propria of the mucosa
- forms papillae, bulging in epithelium
1 _______________________________________________________ - contain glands and lymphatic tissue
Page 79 of 125
Task 118 Task 119 TONGUE OF THE RABBIT. Task 120 TOOTH STRUCTURE AND
DEVELOPMENT SOURCES OF FOLIATE PAPILLAE TISSULAR COMPOSITION
DIGESTIVE SYSTEM Staining Hematoxylin-eosin
Magnification 80Х, 400Х Designate and
complete:
1 – crown, consisting of:
а)
б)
в)
2 – neck of the tooth;
3 – root, consising of
а)
б)
в)
Dental ligaments
(periodontal membrane),
Colour: consisting of:
foregut– in blue; 4 – gum (gingival);
midgut– in yellow; 5 – alveolar bone;
hindgut – in red 6 – periodontium;
Designate: 7 – cementum.
Designate and colour after studying the
1 – oral groove;
2 – oropharyngeal membrane; preparation:
3 – cloacal membrane;
I. Mucous membrane;
4 – pharyngeal recesses; Colour the tissues of the tooth in the scheme:
1 – stratified nonkeratinized squamous epithelium;
5 – thyroid gland rudiment; epithelial tissue– in green, osseous tissue – in blue, fibrous
2 – taste buds in epithelium;
connective tissue – in yellow.
6 – oesophageal rudiment; 3 – lamina propria of the mucous membrane;
tissue regeneration degree
7 – respiratory system rudiment; 4 – primary connective-tissue papilla;
enamel
8 – stomach rudiment; 5 – secondary connective-tissue papillae;
II. Muscular coat (muscular body): dentine
9 – hepatic diverticulum;
6 – striated muscles of the tongue; cementum
10 – pancreatic rudiment; 7 – secretory portions of the glands;
11 – yolk sac; pulp
8 – excretory duct of salivary gland ;
12 – allantois. 9 – fat (adipose) cells; periodontium
10 – blood vessels.
Page 80 of 125
Task 121 TOOTH Task 122
TOOTH DEVELOPMENT. LATE STAGE.
DEVELOPMENT. Staining Hematoxylin-eosin
EARLY STAGE Magnification 80Х, 400Х
Staining Hematoxylin-eosin
Magnification
80Х, 400Х
Designate:
1– oral epithelium; 1 – external enamel epithelium;
2– mesenchyma; Complete the table and write down dental
3– blood vessels;
2 – enameloblasts;
4– dental lamina; 3 – enamel; tissues developing:
5– external enamel epithelium; 4 – odontoblasts; 1. From enamel organ:
6– internal enamel epitheliaum; 5 – predentine; – from internal layer:
6 – dentine; – from external layer and
7– pulp of enamel organ; pulp:
8 – dental papilla; 7 – dentinal canals; 2 From dental bulb.: 1.
9 – dental sac; 8 – pulp; 2.
10 – dental alveolar rudiment.
9 – blood vessels. 3. From dental sac: 1.
2.
Page 81 of 125
Task 123 CROSS- SECTION OF THE OESOPHAGUS DIGESTIVE TUBE
Staining Hematoxylin-eosin
Task 124
(scheme)
Magnification
400Х
Draw and designate:
I – mucous membrane:
1 –stratified nonkeratinized squamous
epithelium;
2 – excretory ducts of proper glands;
3 – muscular lamina;
II – submucous membrane:
4 – proper glands;
III – muscular layer:
5 – a) circular and b) longitudinal
layers;
6 – inermuscular ganglion;
IV – adventitial membrane:
7 – connective tissue;
8 – blood vessels;
9 – nerve fibers.
Page 82 of 125
Designate:
I – mucous membrane:
1 – epithelium;
2 – lamina propria;
3 – lymphatic follicle in lamina propria;
4 – muscular lamina;
5 – crypts;
6 – villi;
7 – fold.
II – submucous membrane:
8 – complex glands.
III – muscular layer:
9 – external circular layer;
10 – external longitudinal layer; Correct tasks №№________________
IV – external layer. The tasks are completed
11 – glands out of digestive tube. Teacher_______________
Date __________________
Page 83 of 125
Topic DIGESTIVE SYSTEM (STOMACH, INTESTINES)
Stomach: sourses of development, general characteristics of its membranes, Write down the organs of digestive tract:
functions.
I. Organs of alimentary canal
Morphological characteristics of different gastric parts: relief of gastric mucosa,
glands (branching, character of secretion, cellular structure), peculiarities of 1.
muscular membrane. 2.
Main gastric glands: topography, characteristics of exeretory duct and secretory 3.
portion (degree of branching, form), cellular structure (cell localization,
4.
micro- and ultramicroscopic structure, functions).
Structural peculiarities of the gastric wall and its glands in the cardiac and 5.
pyloric regions. 6.
Structure and functions of the small and large intestines.
Microscopic structure of small intestine villi, their functions. Ultramicroscopic
II. Digestive glands
structure and regeneration of the small intestine epithelium.
Cavernous, membranous and intracellular digestion. Histophysiology of the 1.
“crypt- fillus” system. a)
Structural peculiarities of the duodenal wall. b)
Morphology of the large intestine wall.
c)
Structural peculiarities of the appendix.
Endocrine system of digestive tract. 2.
3.
Definition of the term “Digestion”_________________________________
____________________________________________________________
____________________________________________________________
Page 84 of 125
Task 125 FUNDUS OF THE STOMACH Task 126 PYLORIC PORTION OF THE STOMACH
Staining Hematoxylin and congo-red Staining Hematoxylin-eosin
Magnification 400Х Magnification 400Х
Draw and designate:
I. Mucous membrane:
1 – gastric fossae;
2 – prismatic integumentary
epithelium;
3 – parietal exocrinocyte;
4 – basic exocrinocyte;
5 – cervical mucocyte;
6 – muscular lamina.
II. Submucosal layer:
7 – blood vessels
Page 85 of 125
Task 127 MUCOUS EPITHELIAL CELLS OF GASTRIC FUNDUS Task 128 DIAGRAM OF IgA SECRETION
(based on electron microscopic image) AND TRANSPORT
Designate:
IV – mucocyte in the proper gland body:
I – superficial gastric epitheliocytes: 7 – mucous secretion granules;
1 – granules of mucous secretion; V – parietal exocrinocyte:
2 – close contact; 8 – intracellular secretory tubule;
II – cervical mucocyte: 9 – vacuoles;
4 – secretory granules of different types; VI – gastrointestinal endocrinocyte:
III – basic exocrinocyte: 10 – secretion granules.
5 – protein secretion granules;
6 – granular endoplasmic reticulum;
Page 86 of 125
Task 129 JEJUNUM (transverse section) Task 130
Staining Hematoxylin-eosin “CRYPT-VILLUS” SYSTEM
Magnification 80Х, 400Х
Designate and colour affers
studying the preparation:
I – mucous membrane:
1 – villi;
2 – crypts;
3 – limbic epitheliocytes;
4 – goblet cells;
5 – proper lamina of the mucous
membrane;
6 – muscular lamina of the mucous
membrane;
II– submucous layer:
7 – nerve plexus node (Meisner’s
node);
8 – blood vessels;
III – Muscular layer:
9 – internal circular layer;
10 – external longitudinal layer;
11 – intermuscular nerve plexus
node (Auerbach’s node);
IV – serous coat:
12 – connective tissue lamina;
13 – mesothelium.
Page 87 of 125
Task 131 DUODENUM Task 132 ТОЛСТАЯ КИШКА
Staining Hematoxylin-eosin Staining Hematoxylin-eosin
Magnification 80Х, 400Х Magnification 400Х
Draw and designate:
I – mucous membrane:
1 – villus;
2 – crypts;
3 – single-layer cylindrical
limbic epithelium;
4 – proper lamina of mucous
membrane;
5 – muscular lamina of
mucous membrane;
II – submucous layer:
7 – circular layer;
8 – longitudinal layer;
9 – nerve plexus node;
IV – serous / adventitial coat:
Draw the mocous membrane and designate:
10 – connective tissue
lamina; 1 – crypts;
11 – mesothelial cell (in case 2 – limbic cells;
of serous membrane). 3 – goblet cells;
4 – proper lamina of the mucous membrane;
5 – muscular lamina of the mucous membrane;
6 – lymph nodule.
Page 88 of 125
Task 133 ENDOCRINE CELL OF Task 134 HORMONAL ACTIVITY OF INTESTINAL ENDOCRINOCYTES
THE SMALL INTESTINE
Designate:
1– lamina basalis;
2– capillary;
3– nucleus; Designate:
4– secretory granules;
4– Golgi apparatus; 1 – enteroendocrine cells; 2 – adjacent enterocytes; 3 – nerve fibers; 4 – smooth
5 - mitochondria; muscles; 5 – arterioles; 6 – connective tissue cells; 7 – capillaries; 8 – goblet cell.
Page 89 of 125
Task 135 THE LINING EPITHELIUM AND GLANDS IN THE DIFFERENT PARTS OF ALIMENTARY
Complete the table: CANAL
The part of the The lining epithelium The alimentary glands and its localized
alimentary canal
1. Oral cavity
2. Esophagus
3. Stomach
4. Small intestine
5. Large intestine
Page 90 of 125
Topic digestive system: liver, pancreas and LARGE salivary glands
Task136. STRUCTUREAL SCHEME OF
1. Development sources of the liver. Functions of the liver. HEPATIC PORTAL LOBE AND ACINUS
2. Structure of a classical hepatic lobe. The concept of portal lobe and
hepatic acinus.
3. Bile ducts: structure of bile ducts and gallbladder.
4. Functions of the exocrine and endocrine portions of the pancreas/.
5. The pancreas, structure of the exocrine portion of the pancreas: lobes,
terminal parts, excretory ducts.
6. Pancreatic islets. Structure of the endocrine portion of the pancreas..
7. Large salivary glands. Sources of development, structure, functions.
It is recommended to review:
Designate:
1- classic hepartic lobe
2- portal lobe
3- acinus
4- portal canal ( triads)
5- central vein
Page 91 of 125
Task 137. BLOOD SUPPLAY the Task 138. SCHEME OF HEPATIC Task 139. ULTRAMICROSCOPIC
LIVER LOBULAR BLOOD SUPPLY STRUCTURE OF THE LIVER.
1–
2–
3– Designate: Designate:
4–
1– interlobular vein; 1– hepatic sinusoid
2– interlobular artery; 2– endothelial cells
3– interlobular bile duct and capillaries; 3– macrophage
4– central vein. 4– space of Disse
Colour: 5– reticular fibers
6– Ito cell
venous flow – in blue 7– tight junction(zonula occludens)
arterial flow – in red, 8– bile canaliculus
bile ducts – in green
Page 92 of 125
SCHEME OF THE
Task 140. LIVER OF THE PIG Task 141 HUMAN LIVER Task 142. GALLBLADDER
Staining: Hematoxylin-eosin Staining: Hematoxylin-eosin WALL
Magnification: 80х, 400х Magnification: 80х, 400х
Designate: Designate:
1– capsule; I – mucous membrane:
2– interlobular connective tissue; Draw and designate: 1 – epithelial cell
2 – lamina propria;
3– interlobular artery; 1– interlobular artery; II – muscular membrane (coat);
4– interlobular vein, 2– interlobular vein; III – serous membrane (coat):
5– interlobular bile duct; 3– interlobular bile duct; 3 mesothelium.
6– hepatic lobe; 4– central vein;
7– central vein; 5– plates of hepatocytes;
8– interlobular sinusoid capillary. 6– interlobular sinusoid capillary;
7– endothelial cells.
Page 93 of 125
SCHEME OF THE SCHEME OF THE ENDOCRINE
Task 143 PANCREATIC Task 144 PANCREAS Task 145 PART OF PANCREAS
ACINUS Staining: Hemoxylin-eosin :
Magnificati 80х, 400х
on.
Page 94 of 125
Task 148 SCHEME OF THE SALIVARY
Task 146 PAROTID GLAND Task 147 SUBMANDIBULAR
GLAND
Staining Hemoxylin-eosin Staining GLAND
Magnification 80Х, 400Х Magnification Hemoxylin-eosin
400Х
Page 95 of 125
Topic: ENDOCRINE SYSTEM
350. 351. COMPONENTS of ENDOCRINE SYSTEM
1. The principle of organization of endocrine regulation. The conceptions of 352.
hormones, target cells and hormone receptors. 353.
2. Hypothalamus: structure, functions 354.Ι.Central part:
3. Hypophysis. Sourses of development. Cellular structure of adeno- and 355.1.
neurohypophysis.
356.2.
4. Hypothalamic-adenohypophysial and hypothalomic-neurohypophysal
systems, neurohaematic organs. 357.3.
5. Epiphysis. Structure. Function. Role of epiphysis in endocrine system. 358.
6. . Thyroid gland. Tissue and cell structure. Function. Peculiarities of the 359.ΙΙ. Peripheral part:
secretory process in thyrocytes, its regulation. 360.1.
7. . Parathyroid gland. Sourses of development, tissue and cell structure, 361.2.
functions, effect on calcium homeostasis 362.3.
8. Adrenal glands. Sourses and stages of development. Structure of cortex and 363.4.
medullar of gland. Regulation of function. 364.
9. Diffuse endocrine system. Types of hormone producing cells. 365.ΙΙΙ The organs with endocrine and non-endocrine
functions
366.1.
367.2.
368.
369.ΙV. Diffuse endocrine system.
370.
371.
Page 96 of 125
Task 164. SCHEME OF HORMONAL EFFECTS ON Task 165. TERMINOLOGY
TARGET CELLS
Learn the terms and concepts:
ENDOCRINY — universal method of interaction between
cells due to signal molecules – hormones, produced by
endocrine cells.
HORMONE — biologically active substance, circulating in
the body and indicating the regulatory effect on target-cells .
TARGET CELL — a cell able to recognize the presence of
a hormone by means of a specific receptor and to respond by
changing the function mode.
RECEPTORS — genetically determined macromolecules
(proteins, glyco- and lipoproteids), localized in different parts of
the cell (plasmolemma, cytoplasm, nucleus) and specialized in
perceiving biologically important signals of chemical and
physical origin.
LIGAND — a molecule (hormone, growth factor,
cytokine), specifically binding to the receptor.
Designate: Give definition of the terms:
1 – autocriny; 2 – paracriny; 3 – endocriny (distant regulation). Autocriny — _________________________________
Designate with letters:
___________________________________________________
A – inherent to anuclear embryonic and tumor cells;
B – provide regulation of activity level and proliferation ___________________________________________________
processes in the epithelial layer; Paracriny — _________________________________
C – provides integral regulation of the whole body.
___________________________________________________
__________________________________________________
Page 97 of 125
Task 166.HYPOTHALAMO-ADENOHYPOPHYSAL SYSTEM
Mark:
372. Hormones of adenohypophisis 373. Target organs 374. Hormones of neurohypophysis Target organ
Basophilic cells 1.
1.
2. 2.
3.
Acidophilic cells
1.
2.
Adrenocorticotropocytes
1.
Page 98 of 125
Task 167 HYPOPHYSIS Task 168 ADRENAL GLAND
Staining Hematoxylin-eosin Staining Hematoxylin-eosin
Magnification 400Х Magnification 400Х
. 378.
375. 379. Regulation of adrenal gland
376.
1. Zona glomerulosa –
____________________________
____________________________
2. Zona fasciculate -
____________________________
____________________________
3. Zona reticularis-
____________________________
377. ____________________________
Medulla_______________
___
Page 99 of 125
Task 169. SCHEME OF Task 170. THYROID AND PARATHYROID GLANDS
HORMONOGENESIS IN THE Staining Hematoxylin-eosin
THYROID GLAND Magnification 80Х, 400Х
Regulation of thyroid gland
(Fill at home)
Organ____________________
Hormones_________________
Organ____________________
Hormone__________________
Designate: Designate:
1. uptake of circulating iodide; 1. glandular capsule;
2. synthesis of thyroglobulin; 2. follicular epithelium;
3. iodination of the thyroglobulin; 3. interfollicular epithelium;
4. intrafollicular colloid fagocytosis; 4. colloid;
5. fusion of colloid drop with lysosome 5. resorption vacuoles; Organ thyroid gland
6. excretion of thyrotoxin into the capillary; 6. parathyroid glandular cell cords. Hormones_____________
7. closing lamina
1– renal corpuscle;
2– proximal convoluted renal tubule (in dark green)
3– proximal straight renal tubule (in light green);
4– thin tubule of Henle’s loop (in brown)
а) descending part; б) ascending part;
5 – distal straight tubule of Henle’s loop (in grey);
6 – distal convoluted tubule of Henle’s loop (in lilac);
structures of urinary tracts:
7 – collective tubule (in yellow);
8 – papillary canal;
382. Male reproductive system, its structure and Fill in the table at home
functions.
383. Development of the male reproductive organs. Embryonic source Structures of male
reproductive systeme
384. Testicle: development, structure, generative and
Primary sex cords 1.
endocrine functions of the testicle. Hemato-testicular
2.
barrier.
3.
385. Spermatogenesis, its periods, regulation.
386. Seminal ducts of the male reproductive system: Mesonephric tubule 1.
direct tubules, testis reticulum, excretory ducts, 2. paradydimis (rudiment)
epididymic canal, seminal duct, ejaculatory canal.
Development, structure, functions. Mesonephric ducts (Wolf’s 1.
ducts) 2.
387. Accessory glands of the male reproductive system:
3.
prostate (prostatic gland), seminal vesicles, bulb-uretal
4.appendix epidydimis
glands. Development, structure, functions. Age-related
(rudiment)
changes. Seminal fluid, its structure.
С
А
Designate on С 8 epididymis
Designate on B : 1. genital ridges 9 ejaculatory canal;
1– Degenerating mesonephric tubule; 2. mesonephric tubules 10 testicular appendage
2– mesonephric duct; 3. mesonephric duct 11 seminal vesicles;
3– developing rete testis; 4. paramesonephric duct 12 prostatic utricle
4– paramesonephric duct;
5– seminiferous cord; 5. testis
6– tuna albuginea. 6. efferent ductules
7. epididymal appendage
A.
Ι - stage proliferation (spermatogonial
phase):
1 –spermatogonia type A dark;
2 – spermatogonia type A pale;
3 – spermatogonia type B.
ΙΙ - stage growth :
4 - primary spermatocytes;
ΙΙΙ - stage maturation:
5 – secondary spermatocytes;
6 – spermatids.
ΙV - stage of formation (spermatid
phase, spermiogenesis). Draw and designate:
Ι- ductuli efferentes (in the cross-section):
B.
Stage of formation include 4 phase:
1– epithelial cells;
golgi phase – 2– fibrous muscular layer;
cap phase – ΙΙ ductus epididymidis (in the cross-section ):
A acrosomal phase –
maturation phase - 3– epithelium;
4– muscular elastic layer.
Hypothalamus
Adenohypophysis
A
B
B differentiate stage
It is recommended to review the material of the lecture Designate 5- suspensory ligamentum of ovary
6-ovary
«Female reproductive system». A Indifferent stage 7- mesovarium
1- primary sex cord 8- uterine tube
9- paroophoron
2-mesonephrous tubule 10 – epoophoron
3- paramesonephric duct
11 – gartner’s cyst
4-mesonephric duct
INDIFFERENTIATED STAGE 1 1-
1–
2– 2–
3– 2
4– 3–
DIFFERENTIATED birth
4–
puberty
Primary sex cord with primordial germ cells
5– 5–
6–
6–
Paramesonephric duct
7– 7–
8–
Mesonephric duct 8–
9– 4 3
10 –
8
Page 112 of 125
Task 189. OVARY
Staining Hematoxylin-eosin Task 190. OVIDUCT
Magnificatio 80Х,400 Х Staining Hematoxylin-eosin
n Magnification 80Х
Draw and designate:
I – ovarian membranes:
1 – rudimental epithelium;
2 – albugineous membrane.
II – primordial follicles:
3 – primary oocyte;
4 – flattened follicule cells.
III – growing (primary) follicle:
5 – primary oocyte; A
6 – zona pellucida;
7 – granular layer.
IV –secondary follicle:
V -mature follicle:
8 – primary oocyte;
9 – zona pellucida
10 – cumulus oophorus;
11 – granulosa cells; B
Designate:A
12 – corona radiata;
I – mucous membrane:
13 – antrum with follicular fluid; 1 – columnar ciliated epithelium;
14 – theca interna; 2 – lamina properia of the mucous membrane.
15 – theca externa; II – muscular layer;
3 – circular membrane;
V – atretic body; 4 – longitudinal membrane.
VI – yellow body: III – serous coat.
B - columnar epithelium
16 – lutein cells 5 –ciliated cells;
17 – blood capillaries 6 – nonciliated cells;
1 – Hypothalamus
2–
3–
4–
5–
6–
7–
Name the phase of menstruale cycle
8–
9–
10 –
REPEAT:
• Fertilization
• Cleavage
• Gastrulation
• Differentiation of germ layers.
Caused by changing processes of physiological cell fall out of “reverse development” of germ provisory
death structures
B E
C D
C D C
4. 5) Extraembryonic organs___________________________
5.
6.
7.