GROUP 5

BIOLOGICAL CHEMISTRY
BIOLOGICAL CHEMISTRY LABORATORY
LABORATORY

DOCUMENTATION

Mr. Jefren Lloyd Gonzales, RMT

and Mrs. Gigie Viernes, RMT

INSTRUCTORS
 GROUP LEADER
GROUP LEADER

Pabro, Humphrey Gian

GROUP MEMBERS
GROUP MEMBERS

Caparas, Earl Cris

Dizon, Joyce
Lauchengco, Drochelle
Orpilla, Charlotte
Pagaduan, Rubelyn Mae
 EXPERIMENT NUMBER
EXPERIMENT NUMBER 11

General test for

CARBOHYDRATES
 MOLISCH TEST
MOLISCH TEST

1. In this experiment, the first thing we did was to 4. As we well add 1ml of concentrated H2SO4, tilt
prepare the glass wares needed for the Molisch the test container. Allow the liquid to run down the
Test. test tube's side until a layer forms within the glucose
solution.

5. Afterward, we observed that the color formed at

2. For the control tube, we prepare 4ml of distilled the junction of two liquids is ________ color.
water and two drops of Molisch reagent.
Afterwards, we place 1ml of 1% glucose solution in
the test tubes.

6. In the same manner as the result seen in the

procedure 5, we add 1ml to the control tube. Then the
observation goes like this.
3. Then, we next proceed to by adding 1ml of
Molisch reagent and mix the solution by gently
shaking the test tube.

7. As we go through, we repeat the procedure from

procedure 2-6 with 1% sucrose, 1% xylose, 1% lactose,
and 1% starch.
 ACTION OF
ACTION OF ALKALI
ALKALI

1. In this experiment, the first thing we did was to

prepare the glass wares needed for the
Experiment for Action of Alkali.

2. We prapare three test tubes and label it.

A. 1ml of 1% glucose solution + 1ml NaOH to test tube 1.
B. 1ml of 1% fructose solution + 1ml NaOH to test tube 2.
C 11ml of 1% galactose solution + 1ml NaOH to test 3.

3. After adding the solution, we put the three tubes

in the boiling water bath.
 TESTS BASED
TESTS BASED ON
ON THE
THE ABILITY
ABILITY TO
TO FORM
FORM FURFURAL
FURFURAL AND
AND ITS
ITS DERIVATIVE
DERIVATIVE
BIAL'S ORCINOL
BIAL'S ORCINOL TEST
TEST

1. In this experiment, our initial action involved 4.Once the solution for test tube 1 has been
preparing the necessary glassware and prepared, the next step is to add 3 mL of Bial's
equipment for the Bial's Orcinol Test. reagent and 1 mL of 1% sucrose solution to test
tube 2.

2. Once the necessary equipment is prepared, we

then proceed to prepare three labeled test tubes.
5. Next, we placed 3 mL of Bial's reagent and 1
mL of 1% xylose into test tube 3.

3. After completing the labeling, we added 3 mL 6. After concluding the solution preparation
of Bial's reagent and 1 mL of 1% glucose to test process for test tubes 1, 2, and 3, we cautiously
tube 1. heat them using a Bunsen burner until the
solutions reach a boiling point.
 TESTS BASED
TESTS BASED ON
ON THE
THE ABILITY
ABILITY TO
TO FORM
FORM FURFURAL
FURFURAL AND
AND ITS
ITS DERIVATIVE
DERIVATIVE
BIAL'S ORCINOL
BIAL'S ORCINOL TEST
TEST
7. After the solution had boiled, we proceeded to
cool the test tubes to room temperature.
Subsequently, we added 1 ml of amyl alcohol to
each solution.

8. After the experimental procedures were

completed, we observed a change in color.
 MUCIC ACID
MUCIC ACID TEST
TEST

1. In this experiment, the initial step entailed 4. Following the solution preparation process for
preparing the necessary glassware for the Mucic test tubes 1, we then cautiously place the test tube
Acid Test. in a boiling water bath for ten minutes.

5. After boiling the solution, we proceeded to

2. Once the necessary equipment is prepared, we
cool the test tubes to room temperature.
then proceed to prepare two labeled one tube.

6. After the experimental procedures were

3. Once the labeling process is finished, we then completed, we observed and recorded the results.
proceed with the addition of 2 grams of glucose,
10ml of distilled water, and 3 mL of concentrated
HNO3 into test tube 1.
 TESTS BASED
TESTS BASED ON
ON THE
THE REDUCING
REDUCING PROPERTY
PROPERTY OF
OF SUGARS
SUGARS
FEHLING'S TEST
FEHLING'S TEST
1. We dilute 1ml of Fehling's reagent (equal parts 4. We raised the test tube with the solution from the
of Fehling's A and B) in 4 mL of distilled water boiling water bath.

2. We boil 1ml of the diluted solution in a water

bath. 6. After the experimental procedures were
completed, we observed and recorded the results.

3. Then we added 1% glucose drop by drop while

heating. And repeated the test with 1% sucrose, 1%
xylose, and 1% starch
 BENEDICT'S TEST
BENEDICT'S TEST

1. In this experiment, the first thing we did was to 4. We shake the test tubes before we place them in a
prepare 4 Test tubes needed for the Benedict’s boiling water bath for 2 to 3 minutes.
Test

2. After that, we label all tubes accordingly.

5. Then we allow the test tubes to cool at room

temperature and observe for the production of color
and formation of a precipitate. But sadly, all the test
tubes resulted negative in Benedict’s Test or all of it
remained blue in color and doesn’t change.

3. Then, we place 1 mLof 1% glucose solution +1

mL Benedict's reagent to test tube 1, 1 ml of 1%
fructose solution +1 ml Benedict's reagent to test
tube 2, 1 mlof 1% xylose solution +1 mL Benedict's
reagent to test tube 3, and 1 ml of 1% starch
solution +1 ml Benedict's reagent to test tube 7.

6. After the experimental procedures were

completed, we observed and recorded the results.
 BARFOERD'S TEST
BARFOERD'S TEST

1. In this experiment, the first thing we did was to

4. keep in boiling water bath and look for the
prepare the glass wares needed for the
development of brick red participate.
Barfoerd’s Test

2. take 1ml of test sample in dry test tube and take

1ml of distilled water in another tube as a control.

3. add 2ml of barfoed's reagents to all the tubes.