Certificate of Analysis

Certificate of Analysis ID: 1054540500_VM871054_EN

Producer and client: Merck KGaA, Frankfurter Str. 250, 64293 Darmstadt, Germany

Test laboratory: Merck KGaA Qualitätskontrolle für mikrobiologische Produkte

Frankfurter Str. 250, 64293 Darmstadt, Germany

Sample identification: GranuCult®

BRILA (Brilliant‑
‑Green Bile Lactose) Broth acc. ISO 4831,
ISO 4832 and FDA‑ ‑BAM

Ordering number: 1.05454.0500

Lot number: VM871054

Accreditation: The test laboratory of Merck KGaA is accredited by

the German accreditation authority DAkkS as
registered test laboratory D-PL-15185-01-00
according to DIN EN ISO/IEC 17025 for the
performance testing of media for microbiology
according to DIN EN ISO 11133:2014.

Test method: Performance testing of liquid culture media:

Qualitative single tube method (turbidity) for performance testing of
liquid media)

Date of analysis: 2019/03/21

Date of release: 2019/04/03

Minimum shelf life: 2024/03/11

Composition (g/l): Enzymatic digest of casein 10.0; Lactose 10.0; Ox bile 20.0;
Brilliant green 0.0133.

Preparation & sterilization: Dissolve 40 g in 1 l of purified water. Fill into tubes containing Durham
tubes and autoclave 15 min at 121 °C. The Durham tubes shall not
contain any air bubbles after autoclaving.

Application: For the selective enrichment, enumeration and confirmation of

Escherichia coli and other faecal coliform organisms from food and
animal feed, water and other materials.

Storage: Store at +15 °C to +25 °C, dry and tightly closed. Do not use clumped
or discolored medium. Protect from UV light (including sun light).

The reported results refer exclusively to the specified medium, see Certificate of Analysis ID.

Merck KGaA · Frankfurter Straße 250, 64293 Darmstadt, Germany: +49 6151 72-2440
EMD Millipore Corp. · 290 Concord Road, Billerica, MA 01821, USA +1-978-715-4321 1054540500_VM871054_EN
ATCC is a registered trademark of ATCC, Manassas, VA, USA. Lit. No. TN105406EN00 Page 1/2
Certificate of Analysis
Physical parameters Specification Lot value
Appearance (clarity): clear clear

Appearance (color): green green

pH-value (25 °C): 7.0 – 7.4 7.0

Microbiological Performance
Qualitative single tube method (turbidity) for performance testing of liquid media
Specification Lot value
Test strain Growth Gas Growth Gas
Inoculum Growth Gas Inoculum
30 ± 1 °C 44 ± 1 °C
Escherichia coli ≤ 100 CFU + + 13 CFU + + + +
ATCC® 25922
[WDCM 00013]
Escherichia coli ≤ 100 CFU + + 68 CFU + + + +
ATCC® 8739
[WDCM 00012]
Citrobacter freundii ≤ 100 CFU + + 95 CFU + +
ATCC® 43864
[WDCM 00006]
Enterococcus faecalis ≥ 104 CFU partial - 1150000 passes -
ATCC® 19433 inhibition CFU test
[WDCM 00009]
Enterococcus faecalis ≥ 104 CFU partial - 1140000 passes -
ATCC® 29212 inhibition CFU test
[WDCM 00087]
Bacillus cereus ≥ 104 CFU total - 280000 passes -
ATCC® 11778 inhibition CFU test
[WDCM 00001]
Staphylococcus aureus ≥ 104 CFU total - 660000 passes -
ATCC® 6538 inhibition CFU test
[WDCM 00032]

Incubation: Escherichia coli, Citrobacter freundii:

24 ± 2 hours at 30 ± 1 °C, aerobic
other test strains:
48 ± 2 hours at 30 ± 1 °C, aerobic
E.coli additionally 24 ± 2 hours at 44 ± 1 °C, aerobic

Reference medium (inoculum): Tryptic Soy Agar

All test methods listed are according to DIN EN ISO 11133:2014.

Signature:

Dr. Stefanie Fischer

Head of Quality Control Microbiology

Merck KGaA · Frankfurter Straße 250, 64293 Darmstadt, Germany: +49 6151 72-2440
EMD Millipore Corp. · 290 Concord Road, Billerica, MA 01821, USA +1-978-715-4321 1054540500_VM871054_EN
ATCC is a registered trademark of ATCC, Manassas, VA, USA. Lit. No. TN105406EN00 Page 2/2