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ICAR2023 Poster Abstract Submission

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Author Details

First Name Return to Submission Form

Jose

Last Name
Lopez-Martin

Email Address
jalopezmartin@gmail.com

PRESENTER First Name

Jose

PRESENTER Middle Initial

A

PRESENTER Last Name

Lopez-Martin

PRESENTER Email
jalopezmartin@gmail.com

PRESENTER Designation (Ph.D., M.S., etc.)

M.D., Ph.D.

PRESENTER Affiliation
PharmaMar - Virology

PRESENTER City
Colmenar Viejo

PRESENTER State or Province

Madrid

PRESENTER Country
Spain
Co-Author Details

Co-Author

First Name
Fernanda

Middle Name

Last Name
Meira

Email Address
fandrade@pharmamar.com

Co-Author Designation (Ph.D., B.S., etc.)

M.D.

Co-Author Affiliation
PharmaMar - Virology

Co-Author City
Colmenar Viejo

Co-Author State or Province

Madrid

Co-Author Country
Spain

Co-Author

First Name
Elena-Maria

Middle Name

Last Name
Saiz-Lou

Email Address
emsaiz@pharmamar.com

Co-Author Designation (Ph.D., B.S., etc.)

M.D.

Co-Author Affiliation
PharmaMar - Virology

Co-Author City
Colmenar Viejo

Co-Author State or Province

Madrid
Co-Author Country
Spain

Co-Author

First Name
Raquel

Middle Name

Last Name
Lloris

Email Address
rlloris@pharmamar.com

Co-Author Designation (Ph.D., B.S., etc.)

Ph.D.

Co-Author Affiliation
PharmaMar - Virology

Co-Author City
Colmenar Viejo

Co-Author State or Province

Madrid

Co-Author Country
Spain

Co-Author

First Name
Diego

Middle Name

Last Name
Lopez-Mendoza

Email Address
dlmendoza@pharmamar.com

Co-Author Designation (Ph.D., B.S., etc.)

M.D., Ph.D.

Co-Author Affiliation
PharmaMar - Virology

Co-Author City
Colmenar Viejo

Co-Author State or Province

Madrid
Co-Author Country
Spain

Co-Author

First Name
Jose

Middle Name

Last Name
Jimeno

Email Address
jjimeno@pharmamar.com

Co-Author Designation (Ph.D., B.S., etc.)

M.D., Ph.D.

Co-Author Affiliation
PharmaMar - Virology

Co-Author City
Colmenar Viejo

Co-Author State or Province

Madrid

Co-Author Country
Spain

Submission Details

Submission Title
Unveiling Antiviral Mechanisms of Plitidepsin by In-Silico Transcriptomics

Submission Track
Broad Spectrum Antiviral Drugs, Biothreat Viruses, and Pandemic Preparedness

Presentation Type
Poster Presentation

Abstract Text (maximum 1700 characters with spacing)

Plitidepsin is an EF1A-targeting marine antiviral peptide in clinical development (COVID19). No published
transcriptional effects on infected cells are available. We explored public repositories to identify additional
antiviral mechanisms.
Data were retrieved from GEO repository (GSE5681), from SKI-DLCL cells (human diffuse large cell lymphoma),
following 24 h-exposure to either plitidepsin 3 nM (IC50) or DMSO (control), using cDNA microarrays (Affymetrix
chip HG U133A-2), in triplicate assays. (Humeniuk R, et al doi:10.1038/sj.leu.2404911).
We used R 4.0.3 and packages affy, limma, clusterProfiler, DOSE, and enrichplot. Benjamini-Hochberg adjustment
was used for multiple testing [significance (SIG) < 0.05]. Genes were ranked by log2 fold change (logFC) for
gene-set enrichment analysis. Relevant (REL) gene-sets were defined by a Normalized Enrichment absolute
Score greater than 1.96.
Plitidepsin induced SIG differential expression in 4101 from 8018 distinct annotated genes: 15 with REL
upregulation (logFC > 1) and 48 with REL downregulation (logFC < -1), resulting in SIG and REL changes in 90
GO (gene ontology) biological processes, not clearly related to the cell neoplastic condition. We highlight gene
enrichments in negative regulation of viral genome replication, viral process or life cycle; response to type I
interferon; negative enrichment in neutrophil activation and degranulation; negative changes in membranes
and endoplasmic reticulum.
 Plitidepsin at very low nM conc. (clinically feasible) is able to induce transcriptional changes in an eukaryotic cell
line, which might be related to the underlying mechanisms of its antiviral and anti-inflammatory properties

Keyword 1
Plitidepsin

Keyword 2
Antiviral

Keyword 3
Transcriptomics

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