23 C 1mL 23C 0.

75 mL 23C 2ml
Time Absorbance Time Absorbance Time Absorbance
0.00 0.148 0.00 0.172 0.00 0.037
1.30 0.163 2.00 0.207 0.30 0.047
Relation between Absorbance x Time for 23°C with different volumes
2.00 0.193 6.00 0.256 1.00 0.066
2.30 0.214 12.00 0.343 1.30 0.077 1.2
3.00 0.247 20.00 0.428 2.00 0.096
4.00 0.271 30.00 0.527 3.00 0.140
1 f(x)
4.30 0.316 40.50 0.637 4.00 0.18 f(x) == 0.0104055584119146
0.0374410770824827 xx ++ 0.195703691671422
0.0241127980093592
R²
R² == 0.989594679350246
0.99887054857139
5.30 0.322 50.00 0.637 5.30 0.2 0.75 mL
0.8 f(x) = 0.025621283831175 x + 0.16722447445803
6.30 0.348 60.00 0.839 8.00 0.302 R² = 0.98154144925798
Linear (0.75 mL)
1 ml
7.00 0.362 70.00 0.931 10.30 0.401

Absorbance
Linear (1 ml)
8.00 0.371 80.00 1.031 13.00 0.501 0.6
2 mL
8.30 0.382 15.30 0.602 Linear (2 mL)
9.30 0.396 18.15 0.7 0.4
10.00 0.402 20.30 0.8
13.00 0.561 23.30 0.901 0.2
17.00 0.601 26.30 1.01
21.45 0.723 0
26.27 0.803 0.00 10.00 20.00 30.00 40.00 50.00 60.00 70.00 80.00 90.00

30.00 1.030 Cumulative time (min)

28°C 33C 1mL

Time Absorbance Time Absorbance
0.00 0.092 0.30 0.235 Relation between Absorbance x Time for 1mL
0.30 0.117 1.00 0.279
0.60 0.211 1.30 0.329
enzymes with differents temperatures
1.60 0.27 2.00 0.359
2.60 0.377 2.30 0.394
3.60 0.436 3.00 0.436 1.200
4.60 0.495 3.30 0.463 f(x) = 0.0385361425209272 x + 0.249658382142135
R² = 0.923876802444333
5.60 0.562 4.00 0.496 f(x) = 0.0629833286498734 x + 0.242049443198467
1.000 R² = 0.993425736074466
7.60 0.675 4.30 0.511 f(x) = 0.0270941778455893 x + 0.15831679566588
9.60 0.732 4.45 0.528 R² = 0.983444319029798 23°C
11.60 0.772 5.00 0.546 0.800 Linear (23°C)
28 °C
13.60 0.82 5.15 0.562 Linear (28 °C)
Absorbance

15.60 0.87 6.15 0.679 0.600 33 °C

17.60 0.922 7.15 0.722 Linear (33 °C)
19.60 0.971 8.15 0.772
21.60 0.99 9.15 0.82 0.400
23.60 1.071 10.15 0.872
11.15 0.922 0.200
12.15 0.972
13.15 1.08
0.000
0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00

Cumulative time (min)