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Proff. Msuya CS 302 Notes - 2019!1!1
Proff. Msuya CS 302 Notes - 2019!1!1
NOTES CS 302
SEED TECHNOLOGY
PROF. D.G. MSUYA
INTRODUCTION
What is seed?
General scientist’s perspective:
Highly compressed or highly compacted and detached structure of a plant that contains a very small
plantlet which can grow and form the normal plant.
Biologist’s perspective:
Mature fertilized ovule or embryo (mature) from which new plants arise.
Seed is important in sexually propagated crops. Asexual propagation has little importance in terms of
seed, except in apomictic seeds.
QUALITY SEED
In simplest terms seed quality means suitability of seeds for sowing and establishing a sufficiently
performing crop field
It is comprised of 10 attributes (components) of un-equal importance. The importance varies
depending on prevailing circumstances
1. Analytical purity
Is the percentage weight of seed sample that is intact seed of the species named on the label after
laboratory analysis, i.e. how much of it is seed.
It is weighed after separation of all impurities.
Impurities are seeds of other crop species, weed seeds and inert matter such as broken seeds, chaff,
pieces of leaves, soil particles etc.
Seed purity mainly depends on the success of the cleaning operations after harvest. Percentages of
98 or more are expected in most crop species but may be lower in some grasses because of empty
florets which are difficult to remove
2. Species purity
It is the number of seeds of species other than the one indicated in the label for the weight submitted
for analysis; example 2/kg
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4. Cultivar purity
Measures the seed’s genetic quality as developed by the breeder; i.e. freedom from seeds of other
cultivars of the same species.
Cultivars are more certainly identified by examination of growing plants, during DUS (Distinctiveness,
Uniformity, Stability) test
5. Germination capacity
It is the percentage by numbers of pure seeds which produce normal seedlings in a laboratory test. It
indicates potential of a seed lot for establishing in good field conditions. It is influenced by harvesting
and subsequent storage conditions and by the dormancy status of the seed.
Germination capacity can be combined with analytical purity and expressed as one value. The value
is known as PURE LIVE SEED.
Pure live seed indicate how much % by weight of the seed lot consists of seeds of the named species
which are capable of germinating and produce robust seedlings
6. Vigour
Is the ability to establish good seedlings even under poor conditions. It is usually associated with high
germination capacity but seeds of high germination may have low % of vigorous seeds. Vogour can
be influenced by:- Damage to embryo incurred during harvesting or subsequent processing
- Environment and nutrition of the mother plant
- Stage of maturity at harvest
- Seed size
- Storage conditions, for example humidity and presence of pathogens
- Genetic factors, for example Hybrid cultivars
7. Seed size
Large seed size is an indication of vigour. The bigger the seed the lager the size of the seedling and
leaf area for photosysnthesis. Larger seeds have greater potential to emerge if deeply buried. Small,
shriveled seeds have no practical planting value.
Seed size is usually expressed as weight or percentage of 100 or 1000 seeds.
Uniformity of seed size influences:- The effectiveness of seed cleaning operations
- Uniformity of growth of seedling
- Effectiveness of mechanical drilling during planting
8. Uniformity
It is nearness to identical characteristics when samples are drawn from different points in the same
lot, i.e. seed size, colour, shape etc. It may vary due to poor grading and mixtures.
9. Seed health
Presence or absence of seed-borne pathogen in the seed; that is seed-borne fungi, bacteria and
viruses. It is best checked by harvesting seed from healthy plants only. This is achieved by imposing
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certification standards of seed health either based on incidence of diseases in the crop or on laboratory
tests of seeds to be certified.
GERMINATION
It is the resumption of active growth of the embryo that results in the rupture of the seed coat leading
to the emergence of a young plant. As it emerges the young plant is called a radical.
Prior to germination a viable seed is rather in-active and the metabolic processes are to a large extent
arrested until conditions become favourable for germination. Some seeds can germinate soon after
harvest or even before their normal harvesting time. Others may be dormant and require an extended
resting period or additional development before germination can occur.
Imbibition
It is a process whereby water passes through natural openings in the seed coat then diffusing into the
seed tissues. The imbibed water performs several functions:-
• Causes cells to become turgid and colloids to be hydrated causing the seed to swell
• Softens the seed coat rendering it more permeable to oxygen and carbon dioxide
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• The swelling causes rupture of the softened seed coat
• Stimulates enzymatic activity
Enzyme activation
When water is imbibed three types of enzymes are activated:-
- Enzymes that aid transfer of nutrients from storage tissues to growing points
- Enzymes that break down stored food in tissues
- Enzymes that trigger chemical reactions for synthesis of new materials
Embryo growth
It is growth of the root-shoot axis that results into differentiation of the epicotyl, hypocotyls and radical.
It occurs at the expense of the storage tissues which are degenerated to release food reserves.
Depending on species, embryo growth may be by cell division or cell elongation. In species like lettuce
root protrusion is primarily the result of cell elongation.
Seedling establishment
This starts when seedlings begin water uptake and photosynthesis. This is when the seedlings
become firmly supported in the soil medium.
Water
Is especially important for enzyme activation. The amount required for seed to start germination
depends on species.
• Optimum amount is that which completely saturates the seed
• Maize germination begins germination when seed absorbs 30.5% of its own weight of water.
The seed is saturated at 43% m.c.
• Extreme moisture may inhibit germination as in dwarf bean and sugar beet
Oxygen
It is necessary for respiration. Most species require the normal atmospheric oxygen concentration
(21%). Few species germinate better at higher or lower concentrations.
• Nitrogen has no effect
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• Carbon dioxide concentration above 0.03% hinders germination while lower concentration has
no effect.
Temperature
A specific continuous or alternating temperature range may be necessary for germination.
Temperature influences the rate of water uptake and metabolism. Response depends on species and
sometimes cultivar and time from harvest (thermo-dormancy). Optimum for most
species `is between 15 – 30oC, 20 – 30oC for tropical species.
For most species the maximum range is 35 – 40oC. Minimum can be down to 0oC. Some species
require diurnally fluctuating (during the day) temperatures for optimum germination, for example many
tree and grass species. The need for fluctuating temperature is associated with dormancy
Light
It is a requirement for some species. The mechanism is similar to that controlling floral induction, stem
elongation and formation of pigments in certain fruits and leaves, radical development in certain
seedlings and unfolding of the epicotyls in bean seedlings. Both light quantity and
quality are important.
SEED DORMANCY
Dormancy is a period of rest in the life cycle of plants during which growth is suspended. Dormant
seeds fail to germinate in favourable environmental conditions (favourable temperature and water
supply). However, such seeds will germinate under similar conditions after a storage period of time
up to several months.
SEED PROCESSING
It is the preparation of seed after harvest for eventual marketing and distribution. It is one of most
important and most difficulty functions of seed business
Basically seed processing has 5 fundamental objectives/purposes/functions
(1) To free good seed from adulterants (cleaning)
(2) To grade for size
(3) To dry seeds
(4) To treat seed with protective chemicals
(5) To prepare lots suitable for handling during marketing (packing)
PRE-CONDITIONING OF SEEDS
Refers to those activities or operations that prepare seed for cleaning; example shelling, de-awing,
threshing etc. (Shelling in maize, for example, is a critical operation. To minimize seed damage maize
should be shelled at 16-17% mc.)
SEED DRYING
When seed comes off the thresher or combine harvester it usually possesses more moisture than it
should for safe storage. Maize, for example, is best shelled at 16-17% m.c to minimize damage. Such
seed needs to be dried to required m.c for storage within 24hrs after shelling. Drying is very important
to prevent mould growth. Deterioration of seeds during or even before storage is greatly related to
m.c.
The influence of m.c. on longevity of seeds makes artificial drying mandatory. Normally at m.c between
35-60% the seed may germinate.
At 16% m.c and above heating begins due to in creased rates of respiration and microbial activity.
Mould growth can begin at 12-14% m.c.
It is therefore necessary to dry seed to as low m.c as possible to minimize deterioration losses.
Drying systems:
There are many systems depending on type of seed, weather, amount of seed, availability of
resources for example fuel/power, etc.
Traditional methods
Utilize the sun or natural ventilation. May involve
(1) Threshed seed spread in thin layer on smooth floor
(2) Spreading on straw matting on the floor
(3) The matting on a horizontal platform (vichanja) to improve ventilation and evaporation
(4) Unthreshed inflorescences hung on frames or wooden supports
(5) Unthreshed inflorescences placed in ventilated cribs.
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1. BATCH DRIER
This is a bin with perforated floor through which air blows. The dry air is blown through a layer of
seed until drying is completed. The drier is then emptied and a new batch filled in. It is a simple method
suitable for small quantities of seed, where also it is easy to clean the bin after drying.
Drying temperature:
In artificial drying higher temps are most occasionally used to speed up the drying. Optimum temps
for drying differ according to types of seed.
Normally the optimum temp range will depend on moisture content of the seed. Recommended temp.
maxima for drying most seeds are 32.2oC for seed at 18-30% m.c., 37.8oC for 10-18% m.c. and 43.4oC
for less than 10% m.c. for slow drying. Seed at higher m.c. are dried more slowly to avoid too quick
drawing of moisture from the seed which may lead to seed damage.
Drying time:
This is influenced by many factors:
1. Seed properties: Seed size, m.c., oil content, seed coat permeability
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2. Drying air temperature
3. Drying airflow rate (m3/min/m3 seed)
4. Seed depth.
High temperatures allow quick movement of moisture from seed. Normally, for every 1 oC rise in temp
RH is reduced by about 4%
Drying depth
Affects the rate of bulk drying. Seeds near the heat source usually dry much more quickly than those
near the evaporation surface.
For effective drying in batch drier the following depths have been recommended:-
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STORAGE DRYING (Keeping the seeds dry during storage or equilibrating the seed m.c while
in storage)
Can be practiced for small quantities of seed (<100kg). It is a suitable method for heat-sensitive seeds
such as vegetables.
Many people use dehumidifiers or desiccators..
Dehumidified cabinets:
Dehumidifiers apply the principles of air-conditioning where R.H. is reduced without the application of
heat. They operate in same way as refrigerator but work at higher temperatures; suitably where
ambient temp. is above 210 C. Below 21oC best to use desiccators.
Desiccators
Use silica gel (or other desiccant). They are more effective at lower temps. As temp. falls, ability to
hold water increases.
Storage drying also suitable for large-seeded crops like beans. These need slow drying and air
conditioned rooms are suitable.
Batch driers (high temp driers) will damage the seeds because it pulls water out of the seed too quickly.
PRE-CLEANING OF SEEDS:
Removal of the most bulky materials (larger, sometimes lighter than seed) and rubbish that is most
likely to shake up conveyors and sieves (affect flowability). Those are materials such as pieces of
trash & plant materials, stones, clods, rodent faeces etc. It consists mainly of an air blast and large-
meshed screens or cylinders. The process of removing large particles is called SCALPING. It is best
to pre clean before seed enters the driers.
BASIC CLEANING:
This is actual cleaning. It is necessary for all seeds. It removes all adulterants and impurities: - weed
seeds, other crop seeds, immature seeds, damaged seeds etc.
Basic cleaning is performed by air- screen cleaners, the basic equipment in seed cleaning plants. The
cleaner usually uses 3 cleaning elements
1. Aspiration – Removing light seeds and chaffy materials
2. Scalping – separation of larger materials
3. Grading – separation of smaller particles (undersized, cut, shrivelled, broken seeds)
A typical air screen cleaner has 4 screens. In actual sense the screen provides the means for both
pre-cleaning and basic cleaning.
If the two operations need to be separated this may be done just by changing the sieves. The seed
flows by gravity from the hopper to the feeder mechanism. The feeder directs the seed to an air stream
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and removes light and chaffy materials. Larger particles slide over the scalping screen and are
collected in a trough.
The screens are usually shaken in a reciprocating motion to enhance separation. There could also be
mechanisms to knock the screens from beneath to free trapped seed/particles. Sieves are available
of various hole types/shapes – round-holed, slatted-shaped, oblong, square, triangular-shaped. They
can be changed depending on the kind and number of crops dealt with. There are over 200 screen
specifications (size, shape) in the seed industry.
UPGRADING:
Seed cannot always be adequately processed using air sieve cleaner alone. Sometimes the
differences in size (breadth) between seed and contaminants are not large enough to effect
separation. For example if seeds are of same width sieve cleaners can not adequately separate short
and long seeds because the longer seeds may be tilted from horizontal position and fall through. To
have a more complete separation sometimes the process needs to go beyond the basic cleaning –
i.e. air-screen cleaning. Various machines are available for upgrading seed. These include.
1. Indented cylinders:
This is for separation between long and short seeds e.g. wheat and oat. It has pockets (indentations)
in inner surface and revolves round a sloping axle. Along its length in the upper half there is a fixed
trough. Seed is fed into the higher end of cylinder and slowly moves towards the lower end.
Short seeds are trapped into the pockets while long seeds slide over. As the cylinder rotates the short
seeds are carried into the upper half of the cylinders rotation where they fall by gravity into the troughs.
Long seeds fall out in the lower part of the rotation and remain in the cylinder. The cylinder can be
adapted to have projecting pins instead of indentations. These pins can enter into holes left by insects
(e.g. pulses) on seeds thus picking damaged seeds.
2. Gravity separators:
This is also called the gravity table. It separates on basis of specific gravity (weight), example empty
from full seeds such as in pulses and flax; also stones and heavy objects. Seed is spread in layer over
a perforated plate or table set at a slope. A current of air is blown vertically through the table (deck),
strong enough to lift light seeds slightly off the surface. Heavy seeds remain firm on the deck. The
deck shakes backward & forwards in jerking movements. Each jerk moves heavy seeds slightly up
the slope. Light seed floats in air and tends to more down the slope as it is not affected by jerking
movements.
3. Spiral separator:
Separates flat seeds from round seeds. Flat seeds follow the spirals down the chute, round seeds
slide quickly and off the spirals.
SEED TREATMENT
Practically all seeds come in contact with pathogens or with organs in soil that attack seed and young
seedlings. It is the application of protectant chemical or physical techniques (e.g hot H 20; radiation
etc) to get rid of and prevent colonization of seed by seed-borne pathogens and insect pests. Seed
treatment is: a very common operation in seed processing. Treatment is mostly by chemicals. The
purpose is:
1. To protect seed from seed rot and seedling blight
2. To protect seed against storage pests and soil insects
3. To prevent spread of plant disease.
Treatment may sometimes be optional. It is, however, a good insurance against disease and soil-
borne organisms. Treatment chemicals may be both fungicides and insecticides.
Fungicides
a) Mercurials (Mercuric compounds)
These are most widely used fungicides. They may be organic or in-organic. In-organic forms include
mainly the chlorides and oxides
Mercuric chloride is injurious to most seeds if not used with caution. Over treatment with organic
mercurials is also injurious.
b) Non – mercurials
These can also be organic and in-organic. In-organic forms include CuS04, Cu20, CuC03 and NASCO-
4 (mixture of Zn oxide and Zn hydroxide).
Cuprous oxide prevents seed decay and damping off in vegetables, but it injures lettuce, onion and
crucifers. NASCO-4 is effective in vegetables seed sensitive to CU 20. Captan and thiram are also
non-mercurials.
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Insecticides
May be combined with fungicide or applied individually. They protect against storage and soil insects.
Some may be applied to protect growing plants through systemic action, for example some organic
phosphates in cotton.
Formulations:
- Dusts – Wettable powders (slurry. Soup-like H20 suspension)
- Liquids (quick-wet application method.
Equipment:
SLURRY TREATERS
These are adaptable to all types of seeds and application rates. They possess a metering mechanism
which rates the application. The small amount of H20 added to the seed does change the m.c of seed
as it is lost soon from seed surface: Usually 0.5 – 1% moisture added. No drying is required after
treatment
DRUM MIXERS:
These involve a simple drum e.g. concrete mixer or a home made drum with rotating handle and
auger.
Dipping mechanisms
- For liquid formulations
- Drip chemical on seed as it passes through certain parts e.g. conveyors. Quick-wet seed
treaters
Application of chemical on seed may also be manual by stirring up the mixture (seed + chemical) with
shovel.
Treatment colours:-
Most treatments contain dyes. These serve 3 purposes:-
1. Warning against food uses
2. As a visible means of evaluating completeness of treatment coverage.
3. Company identity
SEED PACKAGING
This may be packaging or bagging. It is the last operation in which seeds are handled in bulk flow. It
involves filling and weighing; attaching labels and certification tags on the bags, sewing the bags.
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Equipment for bagging is usually the bagger weigher. In this equipment an empty bag is suspended
on the weigher, then seed is allowed to flow into the bag by trip lever. As seed flows a scale counter
balance is actuated so as to stop seed flow by the tripping lever when desired weight is reached. The
bag is then removed.
Bagger weigher may be manual, semi-authomatic or automatic. The manual bagger weigher is usually
mounted on a portable platform. Most scales are semi-automatic, attached to the bottom of the
bagging bin. A feed gate is opened manually but may be closed automatically when the weight is
measured. The bagger weigher can pack 4-5 bags of 50kg/min. Automatic scales are mostly used for
small packages e.g. vegetable and lawn seeds.
A combination of the above rules states that “Good storage is achieved when % RH and ambient oF
temp in the storage environment add up to 100”
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8. Storage pests
- Fungi: Penicillium, Aspergillus, Alternasia, Fusarium, Cladosporium and Rhizopus.
Saprophytes
- Insects: Weevils, beetles, moths.
In principle moisture and temperature have great influence on seed deterioration in storage and
therefore greatest attention is paid to them during storage.
1. Ventilation
Ventillation is needed when temp. inside the store is higher than outside. If so ventilating fans are
turned on to expel warm air from the store and draw in cool air.
2. Insulation
It is a very important consideration in construction of storage facilities. Insulator materials are usually
incorporated during store construction, example in walls, floors, roofs. Fibre board glass wool, cock
board etc are among good insulators used. Sometimes false ceilings are provided so as to leave an
air space between storage room ceiling and actual ceiling. Air is also a good insulator. Stores are
usually painted white (silvery) to reflect the incoming radiation and reduce heat absorption.
3. Refrigeration
Low temperatures are very effective in maintaining seed quality even where RH is quite high.
Generally a reliable method of storing small quantities of seed e.g. breeder/nucleus and basic seed;
carryover seed.
The extent to which storage temp can be lowered by refrigeration alone is very limited. As temp drops,
there is a practical minimum level of RH attained, e.g:-
Temp oC 32 27 23 21 19 16 14 10
Min RH % 30 35 40 45 50 60 70 75
It is recommended that storage should not be done where RH exceeds 60%. To be able to achieve
lower temp levels, refrigeration is normally accompanied with dehumidification. Most seeds suffer cold
injury when stored below 4oC
1. Ventilation
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Ventilation (with dry air) prevents development of damp conditions in the store. May be provided
naturally by allowing air vents and louvers in the storage building; or artificially by use of ventilation
fans. In damp areas natural ventilation may be harmful.
2. Moisture-proofing
Moisture – proofing is provided during construction of storage facility. Materials used for moisture –
proofing include polythene (10 mm) asphalt (3 mm) or aluminium foil. A layer of the material is placed
beneath the floor and overlapped with wall moisture proofing. Metal bins are naturally moisture proof.
Small amounts of seed may be stored in moisture – proof containers e.g. drums and plastic containers
.
3. Dehumidification
Is necessity when RH in store exceeds 60%. Two types of dehumidifiers are known:- Refrigeration
dehumidifiers and Desiccant dehumidifiers.
Refrigeration dehumidifiers reduce RH by condensing H20 vapour on the cooling coils. The condensed
H20 or melted ice is then removed from the storage through a small water pipe or a collection pan.
Desiccants use chemicals, usually silica gel or activated alumina. The method is very effective, can
maintain room RH even below 10%. They work best at lower temps.
STORAGE METHODS:
A: Bulk methods
1. Stacks in protected warehouse
In this method seed is packed in bags or sacks which are arranged on racks/platforms in a
warehouse. It is the best method of holding bulk seed e.g. cereals for short periods of time such
as certified seed. Stacks should be constructed with some skill. They must be:-
- At least 30 cm from walls and not less than 10cm from the floor.
- Stack size of about 2m x 2m and may reach 3.5 – 4m tall.
- A clearance of at least 0..5m should be left between the stack and ceiling.
- Space not less than 0.5m between stacks to enable easy manipulation, sampling, pest control
and ventilation.
In ordinary warehouses ventilation may be the only practical and necessary means of controlling the
atmosphere inside. Normally, about 75 – 80% certified seed is sold in the next season after harvest,
within 8 – 9 months. Provided the seed is dry enough (less than 10% mc) it can be adequately stored
in warehouse with rigorous checks in temp. Seed at 9 – 10% mc can be subjected to long term storage
at 20oC and 50% RH.
2. Storage in bins/silos
This is a high capacity method of storage of bulk (example cereal) seed. The method is satisfactory
for long storage, especially in temperate countries. Seed must be well dried. Additional ventilation may
be provided by perforations in the bottom. Bins or silos are usually fitted with thermometers and RH
meters for remote reading.
3. Underground pits
Made of moisture – proof concrete. These have been used successfully in storing cereal seed
(Argentina) and groundnut seed (Tanganyika).
1. Cold rooms
Can offer long storage if RH is low. They are suitable for breeder, nucleus and even basic seed. Very
long storage of germplasm can be undertaken in cold rooms at 5 oC – 10oC maintained at 30% RH.
Cold rooms at 20oC or less under normal air RH (50% or less) can be used for long storage provided
seed m.c doesn’t exceed 9-10%.
3. Dehumidified cabinets
CONCLUSION
Seed m.c and storage temperature are strongest determinants of seed deterioration. Safe mc for
storage can only be determined if temp is known.
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The higher the temp the lower must be the m.c of the seed. For most seeds the safe m.c. is below
13%. In humid climate 9-10% ms is often the highest allowable for many crop seeds. In colder
(temperate) climates up to 16% may be satisfactory.
1. PRIMARY PESTS
a) Those which and can attack undamaged seed. Most species develop in the seeds both before
harvest and during storage. They include:-
. Sitophilus spp (S. zeamais, S. oryzae, S. granaries)
. Colosobruchus spp.
. Acanthoscelides obtectus
. Zabrotes subfasciatus
. Rhizopertha dominica (lesser grain borer)
. Prostephanus truncates (Greater grain borer)
b) Those which cannot attack undamaged dry seed but are able to survive in the seed especially those
lots with high levels of fragments and dusts.
They include:-
. Tribolium spp.
. Cryptolestes spp
. Oryzaephilus spp.
. Tenabroldes spp
. Ephestia spp etc.
2. SECONDARY PESTS
Infest already damaged seeds, and are able to feed on embryo of moist seeds. They include insects
like flour beetles and grain moths. The Angoumois grain moth, however, is a moth but is in
considered to be a primary insect pest of maize
2. Cold storage
Insect activity is impaired below 20oC and stopped below 10oC. The optimum temperature
condition for most insects attacking seed in store lie between 28-35oC.
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3. Insect proofing of storage building
4. Sanitation
- General cleanliness of store
- Before putting seed in store empty the store completely.
- Clean it thoroughly on walls, the floor and any fixed structures and machines.
- Destroy all the cleanings by burning
- White wash the store (white paint) to seal all crevices
- Spray the store with persistent insecticide such as Lindane, Malathion.
. Clean all spilled out seeds and any rubbish accumulating in the store
5. Seed Treatment. Before storage. Pesticide combined and fungicide.
6. Fumigation
May be necessary for insect populations that may build up while seed is already in store. Some insects
are brought into store from infestations that occurred in the field (or another store). Such insects will
multiply inside the store whatever the sanitation, and need to be fumigated.
Fumigants:-
a) Methyl bromide (MeBr)
. Most important fumigant in cold countries
. Most widely used commercial fumigant in mills, warehouses, hips, railway wagons etc.
. Effective against mites, rodents and insects
. Fumigations last 1-2 days
. Toleranc in Trogoderma spp and Tenebroides, resistance in T. confusum.
b) Phosphine (PH3)
- Most imp. Fumigant in hot countries.
- Very toxic to man and a cumulative effect. Explosive.
- Very toxic to insects and mites (except some eggs and pupae e.g. Sitophilus, Trogoderma
and moths e.g. Sitotroga)
- Use in gas tight silo bins, in stacks under tarpaulin, within gas-tight sacks ets.
- Rate 3-4 tables/tone for 3-10 day sin gas-tight containers.
SEED HEALTH
Many serious plant disease arise from infection carried by seed. This may lead to:-
1. Poor stands due to pre-emergence losses and death of seedlings
2. Spread of disease in later stages.
Seed-borne disease are many and crop losses encountered are great.
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Sorghum Ergot (Claviceps spp)
Kerrel smut (Tolyposporium spp) Up to 70%
Milo dse (Sphacelotheca spp) Up to 100%
TEST METHODS:-
1. Direct visual inspection, example for sclerotia
2. Washing the seed with water and microscopic examination of the suspensions
3. Microscopic examination after staining or cleaning procedures
4. Examination of seeds and seedlings after incubation on blotter, rolled paper towel or agar
media
5. Examination of symptoms developed on seedlings growth in soil, sand or similar material
6. Serological tests (based on biochemical reactions)
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7. Tests performed on bioassays (indicator tests; phage tests etc)
8. etc
The blotter and agar methods allow identification of fungi to species level. The seedling symptom test
proves the use value of seed
Serological procedures enable precise identification of bacterial viruses. Choice of method depends
on range of pathogens expected and effectiveness of method for a pathogen.
The blotter method is the most frequently used routine procedure in laboratories. It enhances
sporulation of the fungi hence ease of identification.
Identification of fungi is based on growth characteristics. Sometimes the colour and growth form of a
fungus on agar plate can be indicative of its identity.
SOTARAGE FUNGI
The main aim of seed health testing is to intercept seed-borne pathogens. Occasionally storage fungi
are also intercepted. Seed so intercepted will usually be a subject of seed treatment and possibly
drying. Their effects to the seed grain can h be dramatic if un-attended. (usefulness in certification
manifested in the effects). They are known to be responsible for about 10-30% loss of the grain crop
in store. The fungi can also cause the following damages in seed:-
1. Decrease in germinability
2. Discolouration of seed and decay
3. Heating and mastiness
4. Loss of weight
5. Various biochemical
6. Production of toxins harmful to man and domestic animals, for example Aflatoxin.
Identity:
Most storage fungi are species of Aspergillus and Penicillium. These are species able to grow without
free water. They are active at RH 70 – 90%
Usually they do not invade before harvest. They may however be on the harvested seed at very low
concentrations. They may be present not only as contaminants but as dormant mycelium within
sometimes the pericarp or seed coat.
Other fungi:
Atternaria, Cladosporium, Fusarium
These are field fungi and are usually the 1st invaders of the stored seed. They are later replaced by
the more thermotolerant species such as Mucor and Absidia; then the typical storage fungi
(Aspergillus, Penicillium).
Predisposing factors:
Mechanical damage in seed, cracks, breaks or scratches in the pericarp of seed coat are conditions
that substantially favour invasion by storage fungi. Other predisposing factors include RH of 65 – 90%
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(and seed m.c corresponding to these humidities); higher temps (> 15 oC) esp. in moist seed(> 12%
mc) and insect infestation.
Minimum RH ranges for storage fungi are 65 – 85% for Aspergillus and 85 – 95% for Penicillium.
Heating may be a source of additional dampness in the seed. It occurs as a result of respiration by
seed, micro organisms and insects, liberating CO 2 and H20. Respiration by micro organisms is by far
the most important; it can raise temperature up to 70 – 75oC. Insect respiration also contribute
substantially to heating and mastiness. Insects, therefore, predispose more microbial growth.
Respiration by insects can liberate enough H20 for seed germination
QUARANTINE ON SEED
Transportation of seed is by all means the most efficient means of spreading plant disease over long
distances. There is practically no distance limit for transportation of seed allover the world. If seeds
were left to the hands of anybody who wants to send them anywhere in the world the problem of plant
disease control would be greatly magnified. Quarantine regulations are necessary to prevent
introduction of new pathogens to new areas. This is plant disease control measure that has been
learnt through years of bitter experience.
Quarantine measures are intended to intercept the infected seed before it spreads the disease. Every
seed importing country has controlling agencies for such interception. In Tanzania quarantine is under
the Plant Protection Division of the Ministry of Agriculture. There is a Plant Quarantine Unit at TPRI
(Tropical Pesticides Research Institute) which is competent for health testing for plant quarantine.
Quarantine officers are also stationed at every border point, airports and in post offices. Any imported
seed material is intercepted at these points. Restrictions are usually put on dangerous pathogens (and
pests) which do not occur or have a limited distribution in the importing country. In this case the
tolerance value is ZERO. Other important pathogens may be allowed a higher degree of tolerance,
example 2%. Seed health test procedures are available for quarantine detection of diseases.
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Quarantine regulations
Most quarantine regulations will involve the following:-
1. Specification of quarantine pests (subjects). Some countries do not specify
2. Pre-entry regulations; for example:-
(a) Phytosanitary certificates
(b) Embargoes; total prohibition of importation of certain kinds of seed or planting material
from certain areas.
(c) Import permits
CERTIFICATION:-
It is a legally sanctioned system for quality control of seed multiplication and production, usually
undertaken by a “Seed certification Agency”
The Agency is usually a Governmental body with the following responsibilities:-
• Establishment of minimum standards
• Registration of varieties for seed multiplication
• Registration of seed growers
• Registration of processing plants for certified seed
• Registration of seed fields
• Field and bin inspection
• Seed sampling and testing
• International agreements for seed certification
CERTIFICATION SYSTEMS:-
- Minimum standards
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- Truth in labeling
STEPS IN CERTIFIATION:-
1. Examination of applications – Verification of seed source
2. Inspection of seed fields
3. Seed sampling
4. Seed testing
5. Tagging and sealing
6. Certificate plot testing
7. Offering of certificates
2. Seed standards
FIELD INSPECTION:-
When to inspect depends on kind of observation to be made: for example botanical characteristics,
diseases etc. It must be before contamination. Selection of inspection points is usually based on a
systematic location of specific of plants/portions within the field in which inspection is concentrated.
The number of field counts depends field size. The number of plants to be observed to complete one
count depends on crop species. Example:-
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________________________________________________________________________________
________________________________
Area (ha) Up to 2 2-4 4-6 6-8 8-10
> 10
Number of counts 5 6 7 8 9 10
The number of plants (heads) per count also depends on plant species.
For bulb crops, cole crops, cotton, curcubits, groundnut, maize, root crops, tomato etc, the number is
100 plants.
For beans, cowpea, greengram, leaf crops, pea, sesame etc, 500 plants.
For barley, oats, paddy, sorghum, wheat, soybean, etc, 1000 plants
There is no limit to the number of rows covered in one count but the number of plants that the rows
may give. The walking pattern in the fields in such that the whole field is represented. The pattern may
however affect area of the field covered. Sensitive areas for inspection are vicinities of the farm bldg,
threshing places, roads into and through the field, where seeds may have been dropped in waste or
in transport.
SEED SAMPLING:-
To know seed quality it is necessary to obtain samples for laboratory tests. The seed forwarded for
laboratory test must be truly representative. For this reason sampling is a key determinant of the true
quality of seed. Sampling must be able to eliminate all possible sources of variability:- sack to sack
variation; within–sack variation. The composite sample must be made up of portion drawn from as
many sacks as possible and as many points from each sack. To be able to draw seed from any points
of the sack, a sampling stick is used.
The composite sample is usually reduced to submitted sample. The rest is retained. The submitted
sample is reduced to working sample,
the rest is retained.
Pre-control:-
Sample of seed lot intended for production of certified seed (i.e foundation seed) is grown for test prior
to distribution of the seed for multiplication.
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Post control:-
Sample of seed certified in previous season is subjected to grow out test to check accuracy and
efficiency of previous certification.
SEED LEGISLATION:-
This means restrictions enforced by penal sanctions. It is the legal basis of seed certification. Aim is
to protect the farmer against un scrupulous seed business; and also the seller when failure cannot be
attributed to him or the seed. Because seed quality is mostly internal, there is ample opportunity for
fraud and deception in seed. The law usually aims to exclude seed of low or uncertain quality from the
market (Europe)
American laws are, however, not restrictive of low quality. They are based on truthful labeling. In
Europe many companies strive to achieve the “Higher Voluntary standards” HVS. This system (HVS)
is now an adopted system in many places
LAW ENOFRCEMENT:-
The responsibility of the seed inspector. The inspector is required to:-
1. Inspect as frequently as may be required necessary all places used for growing, storage or
sale of any seed of any notified kind or variety.
2. Procure and send for analysis if necessary samples of any seeds which he has reason
to suspect, are being produced,
stocked or sold or exhibited for sale in contravention of the law.
HVS
Is a system practiced voluntarily by commercial seed companies to produce seed of quality higher
than the prescribed minimum standard. The companies request the Certification Authority to grant
certification based on the HVS rather than the usual minimum std prescribed. When seeds are certified
and declared to be HVS Certified they attract more market.
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QDS
This is “Quality Declared Seed”. It is a minimum certification system adjusted to suit service needs for
resource poor farmers especially in remote areas. The system is less stringent on certification
requirements. QDS is an artisanal seed certification system. It is “seed produced by a registered
seed producer which conforms to minimum standards for the crop species concerned and
which has been subject to the quality control measures outlined in the guidelines”.
Under the QDS Certification system, the Ctrl authority checks 10% of the seed crop during field
inspection, then checks for seed stds 10% of seed produced for commercial use.
The system tolerates seed produced by small scale informal dealers
Marketing channels/outlets
1. Companies’ own outlets e.g. Branches, Depots (wholesale); retail shops, retail stockists
(supplied by company)
2. Seed merchants; wholesale, retail wholesale merchants may order contracted supplies
3. Governmental/public establishments e.g. Extension offices
4. Non-governmental community organizations, example:-
- Coop Unions
- Farmer Associations e/.g/. TFA
- Farmer/Producer groups e.g. Wami Magole
5. Any other
It is desirable to sell seeds where other agric. Inputs are also sold, more contact with farmers. Retailers
may also be a source of information and advice to seed customers. It is the role of whoever sells seed
to equip them with technical information.
Pricing
Depends on (1) Production cost
(2) Government Agric. Policy e.g. subsidy on inputs.
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Seed promotional activities
Deliberate, planned and sustained activities performed by both the seed suppliers and the extension
service to promote use of good seed.
Functions (of seed promotion service)
1. To inform farmers of the existence and advantages of certified seed
2. To convince farmers to use the seed
3. To avail or advice the farmers on ways and means for requisition of certified seed e.g.
credit, subsidy
4. To train farmers in recommended methods for cultivation of improved seed
5. To provide feedback on usefulness of the certified seed to farmers
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