Mutagenesis

Cloning Exploring
Multiplexing
PCR Mutagenesis

10 9
DNA
Explore Cloning
PCR Gene Expression
Sequencing copies of

SequenchingPCR
applications Genotyping
Gene expression
Pathogen detection
are made after 30
of in 1 reaction
cycles
Multiplexing
d d
a
et to Explore different
fo rg types of PCR
n’t
D o
1 Quantitative PCR
Probe-based
chemistries
AT • 84% of researchers doing qPCR
C use a hot-start DNA polymerase vs.
N
G • 79.1% analyze qPCR data by SYBR™
the standard curve method
d

T Ps technology–based
• 44% use predeveloped assays chemistries

2 Fast PCR
Thermo Scientific™ Phusion™ Flash
High-Fidelity PCR Master Mix
oly
A p me features extension times of 15 s/kb or less.
DN

ras
e

3 Reverse transcription PCR

• Reverse transcriptases are the replicating RNA template
enzymes of retroviruses cDNA
• RT-PCR can happen in a 1-step assay
PCR
or a 2-step assay
MgCl 2
a
mpl te
4 High-fidelity PCR
52x Taq
DNA te

Thermo Scientific™ Phusion™ High-Fidelity

DNA Polymerase is 52x more accurate
than Taq polymerase.
1985 1 Taq
= Fidelity
polymerase error rate
PCR
5 Direct PCR
published
in Science.

PRIM Go straight from sample to DNA amplification.

1989 Taq
ERS

Polymerase is named
“Molecule of the Year”
by Science.

Learn more at thermofisher.com/tsmolbio

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