2460784 C1 RU POCCHHCKAS @EEPALIA @EIEPAJIBHAS CIV KBA TO MHTEIEKTYANIBHOMt COBCTBEHHOCTHL ” RU" 2460 784 C4 (31) MIIK CI2N 5700 (2006.01) (2) OMMCAHME W30BPETEHUA K TATEHTY (21)(22) Basmsa: 2011134609/10, 19.08.2011 (24) Jara navana ore: 19.08.2011 cpora setiers marenra: Fpnopuren(aa): (22) flara nostasn sasmcn: 19.08.2011 (45) Ony6xnxonano: 10.09.2012 Brox. Ne25 (56) Camcox oxysenron, uruponamnsx m orsere © nowexe: ACTA@BEBA E.C. n ap. «ITonysenne hceexoBamHe CYCTeNSHOMHEDX KYIIGTY KxeTOR [AByx usio® rlomHetgtaca: Polyscias fruticosa (L, harms 1 Polyscias fraticosa bailey", 4 cxeaa, oGmectaa yanostoron pacrextt Poccm, Mexaynapomuas xondepens , 18-24 mont 2007, Ciamrstnxap, erp.142, 143. (ew. port) Aapse suis nepennexit 127276, Mocksa, yn. Borarmrueckas, 35, ADP PAH, arentias cnyx6a (72) Anropta): Cyxauona Estena Cepreenuta (RU), Yepnax Haraaus Jlanmronna (RU), Kowxan J[sorrpat Brarusposms (RU), Hocos Amexcanap Muxatironia (RU) (73) Mavenroo6.rautarens(u: ‘exepatsiioe rocystapersenioe GroxKeTHOe yapexaenue nayxu Mneraryr uswonorHH Pactenun wo. K.A. Tumupssena PoccuticKont axaencnn nays (A@P PAH) (RU) (54) IITAMM KYJIbTHBMPYEMBIX KJIETOK PACTEHMS TIOMCI{MAC KYCTAPHHKOBBIN 6a Ne2 (Polyscias fruticosa (L.) Harms) B YCJIOBHAX IN VITRO - IIPOJLYIKEHT W3OMPEHOMOB (37) Peepar: VooSperene orocures K_ GHotexnostorn, Sasnten —mrrae — Kyswrumupyemnsx pactenus srosncuntac Kycrapunxonsait (Polyscias fnuticosa (L.) Harms) 8 yexomusx in vitro, JeHonNposanniih B Poceuekyio Kosutextgsio Kysnramapyewsix Kaerox Brtcrmex Pacrenmi pi yupexutenmm —PoceutickohAkatewun Hays. Muctrryre — innosorun — Pacresth av K.ATumupssesa PAH no oGosnaventen 64 N2 = mposyyenrr wonpenontos. Takum o6pasom, opt Gout HouyseHa —eyereH MONA — KysLKTypa rerox P.fruticosa co CraGiLtbsKIMIE PocTORDIN napaverpay — 1 —Yeroftunmemt — canrex0M monpenowton, Hao6perenue Mower Guith HeHouBIONINO — UI HoNLySeNH NEHER Snonorase AKTHBHEIS — coetitieMnit MOMIPEHOILLOB, KOTOPKIE IIIMPOKO HHeTOTBAYIOTCH 8 qhIpMayestHsecko —ipowBILTeHocTH KOCMETONOTHH —B_—OTEYTCTAME —_eIOHHOML samcumocrit Lt. 2 ra6, vyslo0grv2z2 Nu Lo2460784 C1 RU 40 | a ewan + =Dw'10,c/0 see = ncn w.81095/tan 2 HanecnocobHocte, % ° 5 10 15 20 Bun cyGkyaTHonpoBaHnn Pocrossie xapakTepucTHKH KYJIBTYpBI Polyscias fruticosa (L.) Harms. ur. (56) (npoaosnxenne): SLIWINSKA et al: "Rapid multiplication of Polyscias filicifolia by secondary somatic embryogenesis", In Vitro Cell, Dev, + Plant, 2008, 44:69-77. op: 2 vyslo0grv2z2 Nu Lo2460784 C1 RU RUSSIAN FEDERATION RU” 2460 7a4 41 (51) Incl CI2N 5100 (2006.01) FEDERAL SERVICE, FOR INTELLECTUAL PROPERTY (2) ABSTRACT OF INVENTION (21)(22) Application: 2011134609/10, 19.08.2011 (72) Inventor(s: ‘Sukhanova Elena Sergeevna (RU), (24) Bifective date for property rights: Chemnjak Netaija Danilovna (RU), 19.08.2011 Kochkin Dmitrij Viadimirovich (RU), Priority Nosov Aleksandr Mikhajlovich (RU) (22) Date of filing: 19.08.2011 (73) Proprietor) (45) Date of publication: 10.09.2012 Bull. 25 Federal'noe gosudarstvennoe bjudzhetnoe uchrezhdenie nauki Institut fiziologii rastenij Mail address: im. K.A. Timirjazova Rossijskoj akademii nauk 127276, Moskva, ul. Botanicheskaja, 35, IFR RAN,| (FR RAN) (RU) Patentnaja sluzhba (54) STRAIN OF CULTIVATED CELLS OF MING ARALIA 6a X62 (Polyscias fruticosa (L.) Harms) IN ‘VITRO - ISOPRENOID PRODUCER (37) Abstract: industry and cosmetology in the absence of seasonal FIELD: medicine. dependence. SUBSTANCE: there is declared a strain of I dwg, 2 tbl cultivated cells of Ming Aralia (Polyscias fruticosa 5 (L.) Harms) in vitro deposited in Russian Collection | of Cultivated Cells of Higher Plants affiliated with Timiryazev Institute of Plant Physiology of Russian Academy of Sciences under 61 No.2 ~ isoprenoid producer. Now it can be seen that there has been produced the suspension P. fruticosa cell culture with stable growth parameters and consistent isoprenoid synthesis. EFFECT: invention may be used for producing 1 — high-value biologically active compounds = ro isoprenoids which are widely used in pharmaceutical ‘aitomiieme op: 3 vyslo0grv2z2 Nu LoRU 2.460784 C1 OGnacts npmMenenna Vso6perenne oTHocHTes K GHOTEXHONOTHH, BMACTHOCTH KYBTHBHPOBAHHMTO KAETOK PACTEHHS NlOsIMCHHACA KYCTAPHHKOBOFO, M MOET GbITb HETIOsIBSOBAHO AUN TONyYEHHs UCHHBIX GHOMIOTHYECKH AKTHBHBIX COeAMHEHHM ~ HGONPEHOHIOB, KOTOPBIE ILMPOKO HcombsylOTes B (apMalleBTHYeCKOHt MPOMBIULIEHHOCTH H KOCMETONOF HH, Yponenb TexnuKit Hexoroppte abropbt HasbibaloT pos nomueupac Polyscias cambiM GoraTbIM Ho COmepAHHIO H MHOTOOGPAsHIO HIONPEHONTOB, {TO TEMAET ero TepeMeKTHBHBIM ATH 1uil CHHTe3a THX COeAHHEHHMI M UIs COBMAHMA HX GHOTEXHONOrHHECKOrO npowsboacrsa (Hostettmann K., Kizu H., Tomimori T, Molluscicidial properties of various saponins, Planta medica, 1982. V.44, p.34-35). pHUKOBBIM Polyscias fruticosa (L.) Harms vertostbayer TOHHSHpyIoULe 1OCOGHOCTS H CONPOTHBNAEMOCTb K MibeKIMOHHBIM 3aGoNeBAHHSIM CpeNCTBO, a TAKE KAK CPeCTRO MPOTHB FONOBOKPY>KeHHA ‘SKCTpAkTbI ITOTO PACTeHHA YCHNMBAIOT JLAKTAWINIO, YeKOPHOT paosa COMeTAHHH € APYTHMH PAcTeHHsAMH HETOMIbSYIOTCH MPOTHB HeBPAIrHH H peBMaTHaMA (Korn A.M. B nowekax cpeerna or peex saGouenannit, CTI6, 3AO HIT a» Ha 5-10 CYTKH, TO OCOGEHHO BEIpArKeHO y KPUBOI, Onnckibaroulell HaMeHeHMe “HCA KneTOK B cycMeHsiM, BosMOxHO, 970 CBAIAHO ¢ HamMAHEM, abyx cynonys1at KieTOK B KysTYpe GO ¢ OCOGEHHOCTEIO THAIN CAXaposbl - ee PACHIEMTEHHEM B Cpere BHIPALUNIBAHNE C AATBHEMLIMM NocHeTOBATEMBHBIM TOTpeGreHMENM Tmoxo3bi H bpykTosbt. 3amesstenHe pocta Ha 12-14 cyTKH KYMbTHBNPOBAHHEE NOsBOMAET Hcnonss0BaTe 2-HerenbuB uMKT BEIpauBanns. Ha 17 cyTKH B KyTBTYpE suteTox P.fruticosa oTMeMeHo Hastato Craqun gerpasamtn. Pocrosste napamerpst (KpoMe HHLeKca PocTa) paccuItTbInAsH Mo cyxoMy Becy GHOMACCEI Pesynstart npegcTapaeHt B T2621 Jas spicoxospibexTHBHOTO xHAKOCTHOFO xpomaTorpadiueckoro (BOKX) anamsa 35 Mr Bo3AYUIHO-cyXoft GHOMACCEI KYTBTYPHI KACTOK IKCTPArUpyIOT 70% BOAHBIM MeTAHOIOM To aelicTBiieM YabTpasByka MPH KOMHATHOM Temmepatype B-TeeHHe 30 Mu. 3aTeM nosyHenHbilt 9xcTpakT ueHTpHbyrHpytoT nut 10 THic.06./aunH 6 mun, CynepHataHT qpuawtpytor uepes HetinoHoRO! ustsTp c HOpamn 0,2 mum (Acrodise, Pepwatsy. BOOKX anamme nponoasr Ha npuGope Perkin Elmer Series 200 (PerkinElmer, CLA), YKOMIVICKTOBAHHOM ABTOMATHMECKHM HEKEKTOPOM, GHHApHBIM FpaIMeHTHBIM HACOCOM H CheKrporpoToMeTpHYeckHM JleTeKTOPOM, PasleseHHe MPOBOAAT Ha KOIOHKe Pecosphere 3CR C18, 83%4,6 mm, 3 miko (PerkinElmer, CLA) np cxopoctn noToKa nozBiwKHOF dasbr 1,5 Ma/Mui, TeMlepatype 25°C H AeTeKTHPOBAHHK Ha ALIMHE BONHBI 207 HM. DsioHpoBaHHE OcYLIeCTBAMIOT B rPaaHeHTHOM PEKHME € HCHONBIOBAHHEM B KAMECTBE KOMTOHEHTOB. HoaBIKHON dasbl aneToHHTpHAa H 8 MM pactBopa KH;PO, 8 Boze. Cocras noaBirkHOt basbi (%e aneToHmTpia) MeHaioT no cneayioMteMy 3aKoHy: 0-3 min - 20%, 3-3,5 met - 20- 2240, 3,5-14,5 Mai - 22-26%, 14,5-15 mutt -26-29%, 15-26 mutt - 29-36%, 26-74 mutt - 36-90%, AKOCTE, op:a “ RU 2.460784 CI 27-29 mint - 90%. OSbem mpoGer 30 MKT. Kommecnentioe corepkanie HLAMDICIYAIBHBIX FANKOIHOB ONpeTeARIOT ¢ MOMOIIIO MeToma BHeUIHeH KANHOpoBKH. B KAMECTBE CTAHAAPTOB HeMONMbSYIOT MHLAMBHAYSUIbHEIC PIMKOSHADI, BLUEMEHHBIe 13 JHCTbEB NOsHCIUHACA ManOpTHHKOMCTHOTO, CTPYKTYpa KOTOpBIX yeTaHopmena Metogom 'H - 1 '3C-SIMP: pacrnoputens cmecs nupuaun-ds/D: npnGop Bruker Avance AV600 (Tepmania), BhyTpeHHH cramiapT TerpamerHMicHstan. Peaystbrarsi wa06perenust Kak BigqHo H3 TaGsHybt 1, wrraMat oGiauIAer BHICOKOH KHSHECTOCOGHOCTbIO - OKONO 95% TIpH CraGWIbHBIX pocTOBBIX XapakTepHCTHKAX. B urTaMMe 6a Ne2 KYJIBTYPBI KNeTOK Hommcunaca KycTapHukosoro Polyscias fruticosa (L.) Harms 6bio oGHapyxxeHo 4 HoaMeyosMa (THMKOAHEI OsteaHONOBO KHesIOTHI) - D, Py, A, Po. Hx KomHsIecTBeHHOE Comepkanne Ha 14 cyTKH KYTBTHBHPOBAHHS TpeHcTaBTeHO B TaGs.2. B peaysbrare ns0pereHna Opula NOKA3AHa BOSMOXHOCTS MOTYEHHA CyCMeHSHOHHOH KYbTYPBI KETO HECTAHAAPTHBIM MYTeM - HANPAMYIO H3 9KeTLIAHTOB, TakHM O6pasoM Gbu1a NomyseHa cycrIeHsHOHHAs KysAbTYpA KleTOK P-fruticosa co CTAGIIBHBIMH POcTOBBIMEL apAaMeTpaM H YCTOMMUBBIM CHHTEIOM HBONPeHOHLIOB, Y1OGCTHO TAKOTO cocoa MOAYYCHHS 9THX COCIMHEHHH COCTOHT B OTCYTCTBHH Ce3OHHOM 3ABHCHMOCTH HX, mpowspogcrsa. Pocroni: napaterpa sytypus ecto Plyscia rosa) Harms. Tain Tloeuremt Suave lHisckeu poo no meey e)R0H Goce 28107 secy chp Gnositecet a7s12 wey EO 21305 [Vesna exopoers poor, er! fossa pcs yanoers oy ra8u08 [Manmamaoe copa Guowaces a TELS [keeenocoGuoen, san. Tata, Comeau rsnxernton w xymrype kaeTOR Polya feos (L.) Harms Toma, wir x. acess 5 55 7 yao, wir eye see Bae 734 2s 3a 16 1a opmysa n306pereHHa [rant kyastusupyemsix kero pacTeHHs rosmenptac KyeTapHHKoBEiti (Polyscias fruticosa (L.) Harms) s yeronitax in vitro, zenonmponannitt 8 Pocenfiexyro Koamexmto KynpTapupyemsix Knerox Bercumx Pactennit npx yupexenm Poccutickoft Axamenm Hayk Huicityte @ianomornn Pacrenuit wm, K.A.Tinmmpssena PAH rio o6oanasennem 6 Ne2 - mpoayuent wompeHonon.