Volume 65 | Supplement 1 | 2019

UNIVERSITY OF MEDICINE, PHARMACY, SCIENCES AND TECHNOLOGY OF TÂRGU MUREȘ

International Congress for Students,

Young Doctors and Pharmacists
MARISIENSIS

Tîrgu Mureș, Romania

27th of March – 31st of March 2019

ISSN: 2068 – 3324 • Online ISSN: 2247-6113 • www.actamedicamarisiensis.ro

Volume 65 | Supplement 1 | 2019

International Congress
for Students, Young Doctors
and Pharmacists
MARISIENSIS

Tîrgu Mureș, Romania

27th of March – 31st of March 2019

Disclaimer
The professional and the grammatical quality of the abstracts is the author’s responsibility.
Contributions have been editorially modified. Text has not undergone proofreading.
 PRECLINICAL DENTAL MEDICINE 45

TOOTHBRUSH, ALLY OR ENEMY?

Donca Melina1, Associate Professor Man Adrian1, Lecturer Mare Anca1
1
UMFST Tîrgu Mureş

Background: Previous studies showed that bacterial plaque adheres to the toothbrush even from the first use. It is
of a particular interest to see if those can cause an actual infection just by using them. Objective: The objective of
the study was to count and identify the bacterial load on toothbrushes, in a given time. Material and methods:
Sterile toothbrushes were provided to 15 volunteers, which were further divided into 3 groups of 5 people
according to how many days they brushed (1day/1 week/15 days). The toothbrushes were collected, bristles cut
(330 mg of each), placed in sterile tubes in 3.3 ml TSB medium and thoroughly vortexed for 10 seconds to displace
the bacteria in solution. Then 50 μl of each sample were inoculated on blood agar plates and incubated for 18-24h
at 37°C. The number of bacterial CFU/g of bristles was calculated. Results: The bacterial colonies were counted
and bacteria were presumptively identified by biochemical tests and Gram staining. In the 1-day group: saprophytic
bacteria: Gram-positive cocci and Gram-negative bacilli (E. coli). In the 1-week group: Gram-negative bacilli. In the
15-days group: Gram-positive and -negative cocci, Gram-negative bacilli, saprophytic flora. In the 1-day group,
only two samples presented a bacterial load of up to 10^5 CFU/g. In the 1-week group, all samples were colonized,
with a bacterial load between 2x10^4 and 4x10^5 CFU/g. Surprisingly, in the 15-days group only two samples were
colonized, but with a very high bacterial load of up to 5x10^5 CFU/g. Conclusions: Pathogenic bacteria were not
found, only saprophytic. However, some species considered opportunistic or part of enteral flora were found, so
attention should be drawn to the condition of toothbrush storage.
Keywords: bacterial load, toothbrush, oral hygiene

​FIGHTING THE GAG REFLEX DURING DENTAL IMPRESSION: LIMITATION

Moldovan Iunia1, Boiti Kinga, Lecturer Molnar Cristina1
1
UMFST Tîrgu Mureş

Background: The gag reflex may appear during dental impression if the dentist is not using proper techniques or if
he uses too much impression material. Objective: Establishing the ability to combat vomiting reflex during dental
impression using non-medical methods. Material and methods: On a group of volunteer 155 students from the
Faculty of Dental Medicine, UMFST Tg-Mureș, who have undergone dental impression with alginate. All this
students also received a questionnaire with 5 questions related to gag reflex. We studied and evaluated the impact
and limits of combating the vomiting effect that it is very often seen during this type of work, through the use of non-
medical methods. These non medical methods include: high leg method, salt crystals, astrigent solutions, the use
of a special impression tray to combat the feeling of vomiting. ଀圀栀攀渀 we applied each non-medical method, the
frequency and intensity of the vomit reflex was monitored. We also observed really carefully the efficiency and the
limitations of the applied methods. With the volunteer students where these methods did not work, we tried to take
the dental impression using elastomeric impression materials. Results: The results gathered from the volunteers
we studied were: 132 clear cases where no vomiting reflex occurred, 16 cases with low or moderat vomiting reflex
and there were 7 cases of extreme, intens vomiting reflex to the point where the dental impression could not be
performed, even if we replaced the impression tray or the impression material. Conclusions: This study was
made on informed and ,, prepared" volunteers from a dental point of view presented a better outcome, that were
not effective in the methodes applied to other categories of patients. The limitation of non-drug antiemetic methods
depend very much on subject compliance, the degree of ,, dental education" and last but not least, the skill of the
therapist involved
Keywords: Gag reflex, Students, Dental impression