Culture… Media… 2x
Solid media
Used to grow things called microorganism
In the field of science, we can find this culture
media
A liquidy solution that supports the growth
better
Of bacteria, fungi, algae, virus, protozoa
Contains nutrients so microbes won’t surrender
So come on, hear us, give us your attention
As culture media comes in three classifications
Physical state, chemical composition, functional
type – so come on let’s learn together
P-H-Y-S-I-C-A-L physical state, here we go,
here we go
The first classification with 3 more subdivision,
now let’s begin… liquid media
These are water based solutions that do not
solidify at temperature above specific given
freezing point
It contains various amounts of needed nutrients
but does not contain some specific gelling agent
Huh?! Gelling agent? Yes, gelling agent!
Such as what, such as what?
Such as gelatine and agar
P-H-Y-S-I-C-A-L 2X
Now let’s proceed… Semi-solid media
It exhibits a clot-like consistency, at a room like
temperature that is ordinary
Contains .5% of agar concentration, or less, to
prevent firm substance production
Used to study bacterial motility, and
microaerophilic bacteria, that you can’t see
Here we are in the last category….
It come in a solidifying agent, such as agar with
1.5 to 2%
With this they will have a firm substance for the
cell
To form discrete colonies, determination,
isolation
And hey, hey, hey, it comes in 2 more forms:
a.) Liquefiable – or reversible
b.) Non-liquefiable – or non-reversible
Hala! Hala! Hala, hala, aahhh! 2x
According to chemical composition we have 2
types of classification. The synthetic and non-
synthetic media. Which is which?
Hi! We are the synthetic media!
Our simplicity, complexity, dependent to – what
supplement is being added to
Our substances are chemically defined, maybe
pure, maybe not for as long as you like
And oh, we forgot the important thing, our
precise chemical composition is
Know, representable unlike you, so if we were
you – bye bye bbobbo!
Hey, hey, hey look what we have here; you’re
synthetic that means you are not real
Yes, you are right we are unknown but atleast
we’re complexier than you bbobbo
It’s neither simple nor pure compound, extract
of animals, plants yeast you’ll found
And hey, hey, hey FYI miss, we support the
growth of complex organism hhiiisssss….
Ahhhh! Functional type
Finally, finally, finally, finaallyy!
Functional type it’s the last, alright
With 6 classifications that made us surprised
First stop… it’s GPM
General Purpose Media, now let’s see
Mixture of nutrients that support the growth, of
non-pathogenic, pathogenic microbes
Peptone water, broth and agar – are some of the
examples of this so far
Among certain groups of microorganism, colony
size, and color
Formation of percipitaion or gas bubbles
It allows the growth of microbes colony, that
demonstrate variations in morphology
Boom boom boom chikabombom 2X
Transport media
For clinical specimens in laboratory

Moving on to number 2 don’t forget this ohh. That needs to be transferred immediately

Enrichment media oh ang saya-saya!
It contains blood and serum as complex
substances, to preserve and protect some needed
nutrients
So microbes will increase I specific number,
without touching the whole population you
desire
Blood agar, blood agar – with 5-10%
Blood volume being added in prep blood agar
2X
And oh, there’s a haemolytic reaction
This media prevents the drying, and ensures that
it is not contaminated
Charcoal, Pike’s medium 2x
This two are the example
Brom Brom Brom Brom ah! 2x
Anaerobic Media
Used for organisms that is specified, in the
presence of oxygen they can’t survive
Need reduced oxidation-reduction potential,
such as vitamin K and hemin supplemental

Alpha! Beta! And gamma haemolysis!
Alpha… Beta… and Gamma haemolysis
Selective media- it contains substances that
encourage the growth of specific target
microorganism and inhibiting the growth of
others
Undergoes boiling to remove dissolved oxygen,
and indicator of oxidation such as resazurin
1% glucose, 0.1% ascorbic acid
0.5% cysteine, that’s the end of our routine!
Hola! Kaming grupoha makatingala

Design to prevent the growth of contaminating Abunda kaayo mi sa kagwapa

bacteria and commensals, approaches includes Ni Maam Mariel kami pwerting liwata
changing in pH of the culture, adding substances
such as antibiotics, dyes and other chemicals Maam, kaloy-i papasara kami
Under this we have Thayer-Martin agar, Daghang salamat muadto na kami!
Mannitol Salt agar, MacConkey’s agar,
Lowenstein-Jensen medium and finally
Sabraud’s dextrose agar

Differential media boom booom

These are designed to show visible differences

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