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Got SIBO Here's Why You Need To Get Your Thiamine Status Checked!
Got SIBO Here's Why You Need To Get Your Thiamine Status Checked!
Got SIBO Here's Why You Need To Get Your Thiamine Status Checked!
Small intestinal bacterial overgrowth (SIBO) is classified according to the types of gasses
produced by microflora occupying the upper gut. The two types of gases are hydrogen, and
methane. However, a new classification has emerged based on relatively recent findings
which is characterized by excess hydrogen sulfide production. Hydrogen sulfide-dominant
SIBO is now becoming recognized an independent entity, and may be very common in people
who have been diagnosed with SIBO. In a previous article, I described the process of
hydrogen sulfide metabolism in detail and presented the reasons for why this might occur as
a beneficial adaptation in the context of glyphosate poisoning.
Hydrogen sulfide is a gas known for its characteristic sulfur-like odor. People passing gas that
smells like rotten egg or cabbage are very familiar with this. This gas is mostly produced as
part of the metabolism of a specific set of bacteria referred to as "sulfate-reducing bacteria",
although it can be synthesized by many other bacteria and is also part of normal human
metabolism in small amounts.
Hydrogen sulfide is established as a potent signalling molecule with both beneficial and
detrimental effects on cells, depending on the type of cell and the concentration of the gas.
In elevated concentrations, one of the ways hydrogen sulfide exerts its toxic effects is
through its interaction with heme-containing proteins, causing severe cytochrome c oxidase
deficiency and mitochondrial dysfunction.
Rumbeiha, Wilson & Whitley, Elizabeth & Anantharam, Poojya & Kim, Dong-Suk & Kanthasamy, Arthi. (2016).
Acute hydrogen sulfide-induced neuropathology and neurological sequelae: Challenges for translational
neuroprotective research. Annals of the New York Academy of Sciences. 1378. 10.1111/nyas.13148.
Its transport is passive, meaning that the gas can freely travel throughout the blood stream
and diffuse directly into cells through the cell membrane. Although hydrogen sulfide can
exert pro-inflammatory effects on cells, it can also be fed through into the mitochondria to be
used converted into energy (ATP) and the bioactive form of sulfur which cells can actually
utilize (sulfate). Sulfate is needed for a wide variety of things including conjugation and
detoxification, blood flow, and structural support.
The conversion of hydrogen sulfide into sulfate involves multiple different steps performed
by enzymes located in the mitochondria which include:
Sulfide-Quinone-Oxidoreductase
Thioreductase
Sulfur-Dioxygenase
Sulfite Oxidase
This suggests that supplementation with CoQ10 may therefore be an unexpectedly useful
adjunct in a protocol designed to support healthy sulfur metabolism.
Side note: Many protocols used to treat problems with sulfur metabolism are aimed at
restricting sulfur-containing foods, such as the amino acids cysteine and methionine.
However, restriction of sulfur amino acids has actually been shown to increase hydrogen
sulfide synthesis via upregulated cystathionine γ‐lyase, along with a concomitant decrease
in the levels of glutathione. Therefore, dietary sulfur restriction is likely not a beneficial
solution in the long-term.
A later step in the process of sulfide metabolism involves the conversion of sulfite, a
cytotoxic metabolic intermediate, into sulfate. As you can seen above, the enzyme
responsible for this is called sulfite oxidase (SUOX). SUOX is dependent on molybdenum as a
cofactor, and in the event of reduced sulfite oxidase activity (due to genetics or cofactor
deficiency), a high influx of hydrogen sulfide into the cell may result in a buildup of
intracellular sulfite.
Elevated sulfite poses many potential risks for the cell, which include damage to proteins,
lipids, and mitochondrial dysfunction. However, the point I would like to focus on next is the
effects it has on thiamine.
Enter: Thiamine
Thiamine (vitamin B1) is a critical component of glucose, amino acid, and lipid metabolism. It
is also needed for the synthesis of NAPH, nucleic acids, and neurotransmitters, along with
facilitating nerve transmission. High concentrations are found in pork, organ meats, fortified
grains, and (high sources), although there is a small amount in most other foods.
Although host gut bacteria are responsible for synthesizing a large quantity of thiamine and
may contribute significantly toward total thiamine intake, there are also groups of bacteria
including Clostridia and Bacillus which produce thiaminases and can destroy thiamine. It is
therefore possible that severe cases of dysbiosis could have a severe impact thiamine status.
Long-term gastrointestinal malabsorption frequently accompanies dysbiosis and SIBO, and is
another factor which has been implicated in thiamine deficiency.
In support of this, in vitro data shows that sulfide reduces the concentration of thiamine in
white blood cells. Furthermore, veterinary data shows that excess dietary sulfur coupled with
a subsequent rise in hydrogen sulfide production increases the requirement for thiamine in
the brain and central nervous system and can eventually lead to secondary thiamine
deficiency. Additionally, thiamine supplementation was shown to protect animals against
sulfide toxicity, which suggests that excess hydrogen sulfide does indeed negatively impact
the integrity of thiamine.
Interestingly, clinical reports have shown that people diagnosed with SIBO are more likely to
be deficient in thiamine. Since thiamine is absorbed via the gastrointestinal tract,
maldigestion and malabsorption likely contribute to this finding. However, it is also possible
that this could relate to the defects in sulfide metabolism described above.
Transketolase activity testing is provided by a laboratory in the UK called Biolab and can be
purchased cheaply, so that is one option. However, the thiamine pyrophosphate effect test is
not currently offered by any lab at the time of writing this article.
Another method for testing thiamine status also exists with reportedly very high sensitivity. It
measures whole-blood thiamine pyrophosphate, which is reported to correlate strongly with
intracellular thiamine concentrations and provide a more accurate representation of
thiamine status. This test is currently offered by LabCorp.
Thiamine Biochemistry - Cornell University
Elevated urinary pyruvate and/or lactate - Thiamine is a cofactor for the PDHC, an enzyme
complex responsible for converting pyruvate into acetyl coA. In thiamine deficiency, PDHC
may be inhibited and this can lead to a buildup of pyruvate, which may also be shunted
toward lactate production. The result is higher levels of pyruvate and lactate.
Elevated alanine - Pyruvate can be used to synthesize alanine, and alanine can also be
converted back into pyruvate to be used in energy production. Again, the conversion of
pyruvate requires adequate PDHC activity, and so thiamine deficiency can result in elevated
alanine along with pyruvate.
Conclusion
Hopefully after reading this, the audience can appreciate how SIBO is particularly relevant to
thiamine metabolism. The purpose of this article was to describe the how SIBO and gut-
related issues can potentially lead to thiamine deficiency, and why people suffering from this
condition would do well to test their thiamine status.
In the case long-term deficiency, thiamine repletion may actually go a long way toward fixing
the initial problem. This is because thiamine deficiency might not only be a consequence, but
also a cause of SIBO in the first place.
In the next article, I will be explaining how chronic thiamine deficiency can be causally linked
with SIBO and other gut dysfunction.