Chapter 7: Operation

7.7.2 Create a Worklist Using LIS-Link

LIS-Link helps to ensure that the instrument is communicating with LIS over the network in order
to determine which tests should be run with which samples. When creating a worklist and
samples fail to scan, LIS will not recognize them.
When running a multi-tube assay (i.e., QFT) and all the samples have not been ordered through
LIS-Link, in order to ensure the run is completed successfully, all tubes for each sample must be
selected otherwise the system will register that the correct number of samples were scanned
but not that the correct number were selected for the run.
1. Open DS-Matrix, click File and click .

2. Ensure that both Use LIS on sample batch box is checked and that <new sample
batch> is selected.

3. Once the samples have been scanned, manually input the samples that failed and
select the appropriate number tubes from the Tubes per Sample menu.

Note: Boolean functionality must be enabled in the software for this option.
Note: The dongle for multi-tube functionality must be connected to the
computer.

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4. A warning message is displayed when duplicate sample IDs are found and it will give
each tube a unique identifier. Click to proceed.

5. Wait for the LIMS Wait Message window to close.

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Chapter 7: Operation

6. Select the name of the assay that is to be run from the Assay list on the right and
click .

7. For samples not recognized by LIS, manually click on the boxes next to the sample
names to ensure a checkmark is in all boxes pertaining to all samples to run on that
assay plate.

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 Chapter 7: Operation

Note: Manually clicking the boxes allows the assay to be run on those
samples, but it will not transmit the results to LIS. It will most likely cause the
entire plate of sample results to fail to transmit and either stay in LIS-Link or
generate an XML choke file causing an RPC Server Error.

8. If other racks are selected, ensure that there is a checkmark in all four boxes
pertaining to all samples for those assays as well.

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Chapter 7: Operation

9. Click when it is complete, then load the consumables and let the test run to
completion.
7.7.3 Specify Dilution Ratios at Runtime
If Allow sample dilutions to be changed at run time is enabled for a pipetting operation (Chapter
6.6.2), the dilution ratio for individual samples can be specified when creating the worklist.

Note. When dilution changes at runtime are enabled for an assay, a dilution
button is displayed adjacent to the checkbox for each sample.

Dilution button for the

corresponding sample

1. Click dilution button for the first sample to display the Set Dilution dialog box.

Figure 140: Set Dilution Dialog Box

2. The Original panel shows the dilution that is currently specified for the sample.
Table 40: Dilution Specifications
Field Procedure to Specify the Dilution Ratio
1 in … Enter the total number of parts (i.e., 1 in 200) for the dilution.
1 to … Enter the number of parts (i.e., 199) of diluent that are used.
Enter the volume (in µL) of diluent that will be dispensed into a microplate
Diluent Volume
well or deep well.
3. Type the updated dilution specifications in the Modified panel of the Set Dilution
dialog box.

4. Click . The dilution button for that sample turns green.

Note: Click to remove the dilution updates that were made and
revert to dilutions programmed in the assay.
5. Repeat Steps 1 to 3 for each sample requiring a dilution change.

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