Pengantar

Teknologi DNA Rekombinan

Aliyah Purwanti, M.Si.
TEK3452 / Tahun Ajaran 2023/2024
 Sebelum kita bahas lebih lanjut
Teknologi DNA Rekombinan tentang teknologi ini, mari kita review
dahulu tentang DNA dan gen …

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DNA, Genes & Chromosomes

DNA, genes and chromosomes work

together to make you who you are.
Chromosomes carry DNA in cells. DNA is
responsible for building and maintaining
your human structure. Genes are
segments of your DNA, which give you
physical characteristics that make you
unique. Together, your body has a
complete instruction manual that tells
your cells how to behave.

https://my.clevelandclinic.org/health/body/23064-dna-genes--chromosomes
Genes
Genes → the building blocks for body that give the instructions to make
proteins that will tell body what types of physical characteristics it should have,
like a hair and eye color. We receive one copy of a gene from each parent (one
from the egg and one from the sperm). There are approximately 20,000 to
25,000 genes in body.

https://mirakind.org/wp-content/uploads/2022/05/where-are-your-genes-1.png
 DNA
Deoxyribonucleic acid (DNA) → the
material that exists in every cell in body
that holds genetic code. It makes up
body’s instruction manual.

https://upload.wikimedia.org/wikipedia/commons/thumb/d/d3/0322_DNA_Nucl
eotides.jpg/500px-0322_DNA_Nucleotides.jpg
DNA vs RNA
PERBEDAAN DNA & RNA
Replikasi DNA
▪ Proses penggandaan rantai ganda DNA
▪ Penggandaan tersebut memanfaatkan enzim DNA polimerase
▪ Proses replikasi DNA dapat pula dilakukan in vitro dalam proses yang
disebut Polimerase Chain Reaction (PCR).

https://www.youtube.com/w
atch?v=ihGgFrAIU_U
https://www.youtube.com/watc
h?v=9trjtLLjw6A
Transkripsi & Translasi
▪ Transkripsi
▪ Proses sintesis RNA (mRNA) dengan
DNA sebagai template/cetakan
▪ Berlangsung di nukleus
▪ Translasi
▪ Penerjemahan ode genetik ke dalam
urutan asam amino
▪ Basa mRNA membentuk rangkaian
kodon (rangkaian 3 basa yang
berdampingan pada mRNA yang
menyandikan satu asam amino)
▪ Berlangsung di sitoplasma →
ribosom
Tahapan
Transkripsi
Tahapan Translasi
 TUNTAS tentang bahan baku utama
teknologi DNA rekombinan, minggu
depan kita bahas bahan – bahan lainnya
ya : (1) plasmid; (2) enzim pemotong; (3)
enzim penyatu; (4) bakteri;
Sekaligus kita bahas prosesnya.
Dipelajari dulu ya.

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