ACTIVITY #2: STAINING

De ne the following words about staining.

1. Autoclave- An autoclave is a device that is a specialized device used in labs, hospitals, and other
healthcare facili es to sterilize tools and supplies by applying high-pressure steam for a
predetermined amount of me.

2. Centrifuge- One of the most o en used pieces of equipment in a biological lab is a centrifuge.
Denser par cles sink to the bo om of a centrifuge tube as a result of the instrument's use of
centrifugal force to separate components in a uid. Par cles are split into layers a er
centrifuga on according to their densi es.

3. Microscope- Using a technique called microscope cell staining, it is possible to view cells and cell
components more clearly under the microscope. A nucleus or a cell wall are easier to see by
employing certain stains. Only a few types of stains can be used on living cells, while the majority
of stains can only be applied to xed, non-living cells.

4. Sterile loops- Tools to transfer clinical microbiology samples securely include inocula ng loops
and needles. It is possible to facilitate uniform slide staining by using the disposable or reusable
instruments.

5. Vaccine bath- By hea ng in a water bath at 60oC for one hour, the contamina ng bacteria in a
vaccine mixture can be rendered inac ve. Spores persist, and only vegeta ve bacteria are
destroyed.

6. Specimen dish-

7. Tuberculin syringe- Tuberculin needles are normally u lized for tuberculosis tes ng or "tb tests."
Tuberculin, a ltered protein subordinate, is a glycerol concentrate of the tubercle bacillus
u lized for tuberculin (PPD) skin tests. A standard por on of tuberculin is infused between the
layers of the skin.

8. Petri dish- a shallow straigh orward glass or plas c round and hollow lidded dish, used to
contain a meager layer of agar on which to develop microbes and parasites. A great many Petri
dishes are u lized in microbial science labs consistently.

9. Desicator- Desiccators are sealable nooks containing desiccants u lized for safeguarding
dampness touchy things, for example, cobalt chloride paper for another u liza on. A typical use
for desiccators is to safeguard synthe c compounds which are hygroscopic or which respond
with water from dampness.

10. Vacuum pump- Vacuum siphons are intended to lessen the tension inside a framework
consequently bringing down the edge of boiling over of a dissolvable. By decreasing the limit,
the dissolvable dissipates at a much lower temperature, which lessens test corrup on.
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