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Surono IS and Hosono A (2011) Fermented Milks | Types and Standards of Identity.
In: Fuquay JW, Fox PF and McSweeney PLH (eds.), Encyclopedia of Dairy
Sciences, Second Edition, vol. 2, pp. 470–476. San Diego: Academic Press.
FERMENTED MILKS
Contents
Types and Standards of Identity
Starter Cultures
Health Effects of Fermented Milks
Buttermilk
Nordic Fermented Milks
Middle Eastern Fermented Milks
Asian Fermented Milks
Koumiss
Kefir
Yogurt: Types and Manufacture
Yogurt: Role of Starter Culture
Originally, the primary function of fermenting milk was Milk fermentations generally involve the metabolism of
to extend its shelf life. With this came numerous advan- lactose to lactic acid, a characteristic common to all fer-
tages, such as an improved taste and enhanced mented milks, by lactic acid bacteria (LAB), mostly
digestibility of the milk, as well as the manufacture of a lactococci and lactobacilli.
wide variety of products. Historically the fermentation of Fermented milk is obtained by fermentation by suita-
milk can be traced back to around 10 000 BC. It is likely ble microorganisms resulting in reduction of pH with or
that fermentation initially arose spontaneously from without coagulation. These starter microorganisms
indigenous microflora found in milk. should be viable, active, and abundant in the product to
Fermented milks have been an important component the date of minimum durability. If the product is heat-
of nutrition and diet. In addition to yogurt, there are treated after fermentation, the requirement for viable
numerous types of fermented milks manufactured in dif- microorganisms does not apply.
ferent parts of the world. Throughout the world, around As long as the starters are handled correctly, and added
400 names are applied to traditional and industrially to the milk of good quality, and the manufacturing tech-
made fermented milk products. In general, the different nology is adhered to, fermentation will produce a stable
types of fermented milks tend to be classified according to distinctive fermented milk product. Standard processes
the methods of fermentation and/or processing, which are are now available for most types of fermented milks.
related to the microorganisms involved. Each type of The basic identity of each type of fermented milk
fermented milk involves specific microorganisms, based results from both the specific microorganisms and the
primarily on the optimum growth requirements of the process conditions providing suitable environment for the
starter cultures (i.e., mesophilic and thermophilic micro- microorganisms concerned. Basic metabolism of the species
flora); however, there are strong similarities between or groups of species plays an important role in supplying
manufacturing technologies used. metabolites that ensure a complex range of chemical
470
Encyclopedia of Dairy Sciences, Second Edition, 2011, Vol. 2, 470-476
Author's personal copy
Fermented Milks | Types and Standards of Identity 471
interactions, producing numerous functional properties strains of mesophilic or thermophilic LAB are used; (2)
such as preserving ability, flavor as well as texture devel- products obtained through alcohol–lactic fermentation,
opment, and thus contributing to the sensory quality of involving yeast and LAB; and (3) products where, in
fermented milk, which in turn improves the nutritional addition to fermentation of type (1) or (2), there is mold
value. growth. All products are the result of fermentation of
Careful selection of species is important in maintain- lactose into mainly lactic acid.
ing the identity of each fermented milk product, with
temperature as an essential factor. Table 1 shows exam-
ples of established types of fermented milk, with the Lactic Fermentation of Milk
microorganisms used as starter cultures. There are two types of lactic fermentation based on the
incubation temperature, namely, mesophilic fermenta-
tion, employing mesophilic starters, and thermophilic
Types of Fermented Milks fermentation, involving thermophilic starters, composed
solely of LAB.
Fermented milk products are classified into three differ- Cultured buttermilk, cultured cream, ‘lactofil’, ‘film-
ent types: (1) products of lactic fermentation, where jölk’, nordic ropy milks, ‘ymer’, ‘shrikhand’, and ‘chakka’
Table 1 Some established types of fermented milk and their starter cultures
Adapted from Kurmann JA and Rasic JLj (1988) Technology of fermented special products. In: Fermented Milks Science and Technology. Bulletin of
the IDF No. 227; Lee YK and Wong SF (1993) Stability of lactic acid bacteria in fermented milk. In: Salminen S and Wright A von (eds.) Lactic Acid
Bacteria, pp. 103–114. New York: Marcel Dekker, Inc.; Tamime AY and Robinson RK (1999) Yoghurt. Science and Technology, 2nd edn. Cambridge,
OK. Woodhead Publishing Limifed.
are the types of products obtained through mesophilic ‘tätmjölk’, produced in Sweden. It has a mild, sour taste
fermentation of milk, while yogurt, ‘laban’, ‘zabady’, and a ropy consistency. A similar fermented milk tradi-
‘labneh’, ‘skyr’, ‘yakult’, acidophilus milk, and Bulgarian tionally made in Norway is called ‘tettemelk’.
buttermilk represent products obtained as a result of ‘Filmjölk’, developed in Sweden in the early 1930s, is a
thermophilic fermentation of milk. sour milk used as a drink, often consumed with meals. It
contains 3.0% fat and it has a characteristic flavor and
Mesophilic fermentations aroma, and a relatively high viscosity.
Cultured buttermilk ‘Ymer’ (Denmark) and ‘lactofil’ (Sweden) are concen-
Cultured buttermilk, a low-acid fermented milk, is a trated after fermentation by removal of a fixed percentage
pasteurized skim milk fermented by a lactic culture and of whey. Both these products are made with nonropy
by aroma-producing bacteria. High-quality cultured but- culture. (see Fermented Milks: Nordic Fermented
termilk has mild acid flavor with an aromatic diacetyl Milks).
overtone and a smooth viscous body and texture. Its appear-
ance is soft white, without gas holes or whey separation, and Thermophilic fermentations
the product remains fresh for at least 10 days at 5 C. Yogurt
Lactococcus lactis subsp. cremoris and Lc. lactis subsp. lactis are Yogurt, a medium-acid fermented milk, is fermented by
responsible for acid production, while citrate-positive symbiotic cultures of Streptococcus thermophilus and
strains of Lc. lactis subsp. lactis biovar diacetylactis and Lactobacillus delbrueckii subsp. bulgaricus characterized by a
Leuconostoc mesenteroides subsp. cremoris are the primary smooth, viscous gel with a delicate walnutty flavor. The
sources of flavor and aroma, because of their ability to two yogurt bacteria reside either naturally in the milk (in
produce diacetyl, an important volatile compound that is the regions where yogurt is traditionally made) or are
critical for cultured buttermilk quality, giving the product added as starter cultures at 2–5% inoculum, preferably
its characteristic buttery flavor and aroma. at a 1:1 ratio. Incubation is at 42–45 C for 3–6 h until pH
Most cultured buttermilks are made with mixed cul- 4.4 and 0.9–1.2% titratable acidity is reached.
tures as shown in Table 1. Milk is fermented at 22 C The delicate flavor of plain yogurt is achieved through
until a titratable acidity of 0.9% is reached. Incubation at a protocooperation relationship between rods and cocci,
22 C is necessary to enable both species of starter cul- which is influenced by factors such as incubation tem-
ture to produce the desirable characteristics of cultured perature and acid concentration. Lactobacillus delbrueckii
buttermilk. Incubation at a higher temperature would subsp. bulgaricus produces amino acids and peptides
favor the growth of Lc. lactis subsp. lactis, resulting in required by Sc. thermophilus as growth factors, while
excess acid production, and diminish aroma production folate is produced by Sc. thermophilus to support the
by Ln. mesenteroides subsp. cremoris. Buttermilks are growth of Lb. delbrueckii subsp. bulgaricus.
most popular in Germany and Scandinavian countries. Volatile compounds include small amounts of acetic
See Fermented Milks: Buttermilk. acid, diacetyl, and acetaldehyde produced by
Lb. delbrueckii subsp. bulgaricus, which contributes much to
Cultured cream the unique flavor of yogurt. Heat treatment of yogurt after
Cultured cream or sour cream, a low-acid fermented milk, fermentation is permitted in most countries. Alternative
is an extremely viscous product with the flavor and aroma nutritive sweeteners such as aspartame are also permitted.
of buttermilk. The final pH value of a freshly produced Based on the method of production, there are two types of
sour cream is about 4.5. It normally contains not less than yogurt, namely, set and stirred yogurt.
18% butterfat. The functions of the starter cultures are the Yogurt and yogurt-like products are made widely in
same as in cultured buttermilk, dominated by Lc. lactis. The the Mediterranean area, Asia, Africa, and central Europe.
incubation temperature is 21–24 C. Synonyms for yogurt or related fermented milks through-
out various countries are shown in Table 2. ‘Zabady’ or
Nordic (Scandinavian) fermented milks Egyptian yogurt is traditionally made from sheep’s milk.
Characteristic types of Nordic fermented milks are noted ‘Dahi’ and ‘dadih’ are Indian and Indonesian yogurts,
for their high viscosity and ropiness. They owe these respectively. Buffalo milk is often used in the manufacture
properties to the vigorous growth of capsule-forming of these products, sometimes in combination with bovine
lactococci, mainly Lc. lactis subsp. cremoris. If one applies milk. See Fermented Milks: Asian Fermented Milks;
a needle or a spoon to the milk surface, long strings will Middle Eastern Fermented Milks; Yogurt: Role of
appear when the needle or spoon is lifted. The ropy Starter Culture; and Yogurt: Types and Manufacture.
character of these fermented milks is derived from the
formation of exopolysaccharides formed by the lactococci Bulgarian buttermilk
involved in fermentation. An example of a traditional Bulgarian buttermilk, a high-acid fermented milk, is made
product of this type is ‘långfil’, also referred to as by inoculating Lb. delbrueckii subsp. bulgaricus alone (at 2%
Table 2 Yogurt-related fermented milks in various countries strain Shirota, and the protein content is 1.08%. See
Fermented Milks: Asian Fermented Milks and Health
Synonym Country
Effects of Fermented Milks.
Dahi India
Dadih Indonesia
Probiotic fermented milks
Katyk Kazakhstan
Laben, leben Iraq, Lebanon, Egypt Probiotic fermented milks are made with specific
Laben, rayeb Saudi Arabia strains of various probiotic bacteria, including bifidobac-
Mast Iran, Iraq, Afghanistan teria. Some strains of Lb. acidophilus, Lb. casei, and
Matzoon, madzoon Armenia Bifidobacterium spp. are the most commonly used probiotic
Roba, rob Egypt, Sudan, Iraq
bacteria in the manufacture of fermented milks. These
Tarho Hungary
Tiaourti Greece and some other microorganisms are thought to confer
Yaourt Russia, Bulgaria health and nutritional benefits, through their activity in
Zabady, zabade Egypt, Sudan the intestinal tract. The traditional yogurt starter cultures
Sc. thermophilus and Lb. delbrueckii subsp. bulgaricus do not
grow in the intestinal tract. However, they do improve the
inoculum) into pasteurized whole milk and incubating at utilization of lactose in persons who are lactose maldiges-
38–42 C for 10–12 h, until a curd forms with about 1.4% tors, and some probiotic fermented milks are processed in
titratable acidity, which gives it a sharp acidic flavor. This a similar manner to yogurt, fermented by yogurt starter
product is popular only in Bulgaria. cultures enriched with probiotic bacteria.
The number of fermented milks made with probiotic
microorganisms has increased markedly over the past few
Acidophilus milk
decades. Such fermented milks are usually made with
Acidophilus milk is cultured with Lactobacillus acidophilus,
more than one organism although products made with
whose primary function is to produce lactic acid from
single-strain cultures are also known.
lactose. Moreover, Lb. acidophilus is considered to be a
In Germany for example, fermented milks made with
probiotic bacterium, and has been claimed to confer var-
probiotic bacteria include ‘bifighurt’, ‘bioghurt’, and ‘bio-
ious nutritional and health benefits on consumers. An
garde’, while a product called ‘cultura’, made with
ability to grow in the presence of acid and bile acids
Bifidobacterium bifidum and Lb. acidophilus, originated in
enables it to survive in the intestinal tract.
Denmark. Often, fermented milks presently manufac-
Lactobacillus acidophilus grows only slowly in milk and
tured in various regions and countries reflect local
therefore it is essential to maintain the inoculum’s activity
traditions of homemade fermented milks.
by daily transfers of mother culture; this will help to
The success of fermented milk products relates to
ensure consistent results. Acidophilus milk is fermented
nutrition and health, versatility, and marketing.
at 38 C with the inoculum of 2–5% active culture, until a
Scientific and clinical evidence is also mounting to sup-
curd forms (which usually happens after 18–24 h).
port the consumer perception of health from fermented
The final product contains 1.5–2.0% lactic acid but no
milks. See Bacteria, Beneficial: Probiotics, Applications
alcohol. It is cooled to 10 C before agitation and pumped
in Dairy Products and Fermented Milks: Health Effects
to a filler where it is filled into bottles or cartons.
of Fermented Milks.
In some countries, doctors recommend acidophilus
milk to patients with various gastrointestinal tract disor-
ders including constipation, nonulcerative colitis, and
diarrhea. See Fermented Milks: Health Effects of Yeast–Lactic Fermentations
Fermented Milks. Kefir
Kefir, an acidic and alcoholic fermented milk, has a sour
Yakult cream-like consistency and a distinct flavor. This foamy,
Yakult is renowned in Japan mainly on account of claims effervescent drink containing 0.9–1.1% lactic acid and
of its health-promoting properties. It is made with 0.3–1.0% alcohol has achieved great popularity in eastern
Lactobacillus casei subsp. casei strain Shirota, which appears Europe, but its consumption is limited elsewhere, which is
to remain viable in the intestinal tract of humans and is in part due to problems with packaging and distribution
also a probiotic bacterium. (if low temperature is not maintained, yeasts present in
The milk solids content in yakult is 3.6%, but it has kefir will continue to produce alcohol and carbon dioxide
16% added sucrose. Yakult is a Drink based on during storage).
Fermented Milk, consisting of 40% fermented milk, Kefir is usually made using whole milk by subjecting it
which is fermented by a single-strain culture. The final to severe heat treatment (95 C for 5 min) to denature the
product contains 108 cfu ml 1 viable Lb. casei subsp. casei whey proteins and therefore improve the viscosity of the
Puhan Z (1988) Results of the questionnaire 1785B ‘Fermented Milks’. Tamime AY (ed.) (2006) Fermented Milks. Blackwell Publishing Ltd.
In: Fermented Milks – Science and Technology. Bulletin of the IDF Tamime AY and Marshall VME (1997) Microbiology and technology of
No. 227. fermented milks. In: Law BA (ed.) Microbiology and Biochemistry of
Robinson RK and Tamime AY (1990) Microbiology of fermented milks. Cheese and Fermented Milk, pp. 57–152. London: Blackie
In: Robinson RK (ed.) Dairy Microbiology, Vol. 2: The Microbiology of Academic and Professional.
Milk Products, 2nd edn., pp. 291–343. London: Elsevier Applied Tamime AY and Robinson RK (1999) Yoghurt: Science and Technology,
Science, pp. 138–164. Brussels: IDF. 2nd edn. Cambridge, UK: Woodhead Publishing Limited.