INTRODUCTION

Each and every bacterium causes good and bad not only in our environment but also
inside a human body. The provision of antimicrobial agents is crucial because some of them
have become resistant. The researchers would like to figure out the effect of carabao grass
(Paspalum conjugatum) extract and garden snail (Cornus aspersum) mucin against a specific
bacteria called the Enterobacteriaceae.

Background of the study

Adaptation is one of the characteristics of a bacterium. Living organisms somehow do

that to survive and to adapt to what is currently beneficial to them. Striving to live and
reproduce according to their own needs. The challenge is that overpopulation is not good,
specifically when talking about bacteria. The researchers have chosen to the selection of
bacteria which is the Enterobacteriaceae spp. in determining the destructive effect on it to the
environment. Of course, the researchers will not let bacteria dominate because of the damage
they cause externally and internally knowing that some of them might cause timely sickness
such as fatigue, chills, fever, headache and many more to mention. Some become resistant due
to adaptation to environmental changes and human activities like taking antibiotics that are not
prescribed. Bacteria tend to improve like humans and other organisms do. Abominations
continue to grow, knowing that the damage caused by bacteria might lead to loss of life if not
treated rightfully and immediately. The only solution on maintaining the equilibrium is by using
antimicrobial agents. The researchers devised a method for improving a specific agent's
antimicrobial activity by combining the synergistic effects of two different agents to provide
much more effective extermination of life-threatening bacteria. Coping up mechanism is a
normal human characteristic, so the researchers devised a method of determining the
effectiveness of carabao grass (Paspalum conjugatum) extract and garden snail (Cornus
aspersum) mucin on the specific bacterium Enterobacteriaceae.

Enterobacteriaceae are gram-negative type of bacteria with more than 100 species.
According to research, some members of the Enterobacteriaceae produce endotoxins that,
when released into the bloodstream following cell lysis (destruction), cause a systemic
inflammatory and vasodilatory response. The most severe form of this is known as endotoxic
shock, which can be rapidly fatal. The researchers came up with the idea of exterminating some
enterobacteriaceae spp. through addition of certain antimicrobial agents together in the
formation of more potent and lethal agent to stop the multiplication of bacterial organism.
The researchers would like to know the effect of carabao grass (Paspalum conjugatum) extract
in addition to garden snail (Cornus aspersum) mucin in terms of behavioral activity of the
enterobacteriaceae. The researchers will then gather up all the information included in the
experimental activity to prove the effectivity of carabao grass extract and garden snail mucin if
the bacterial multiplication is going to lessen or exterminate the bacteria itself.
 Statement of the Problem

1. To learn how effective the antimicrobial synergistic effect of Carabao Grass (Paspalum
conjugatum) and garden snail (Cornus aspersum) mucin against Enterobacteriaceae

2. To learn efficiency of the individual effect of garden snail (Cornus aspersum) mucin and
carabao grass (Paspalum conjugatum) extracts as an antimicrobial agent against
Enterobacteriaceae

3. To learn the difference between the synergistic effects of garden snail (Cornus aspersum)
mucin and carabao grass (Paspalum conjugatum) extracts compared to their individual effects as
an antimicrobial agent