ADENOVIRUS 50 Q

MURRAY CHAPTER 42 P.418-424

BY: GE_AWAEH

1. What is the family of Adenovirus?

Adenoviridae
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2. What is Adenovirus genome?

The adenovirus genome is a linear double-stranded DNA with a terminal protein
(molecular mass, 55 kDa) covalently attached at each 5′ end.
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3. What is the mode of viral transmission from Adenovirus:#1. Fecal oral

transmission #2. Transmission via blood #3. Genetic #4. Maternal-neonatal
transmission
1. Fecal-oral transmission (Picornaviruses, rotavirus, reovirus, noroviruses,
adenovirus )

2. Transmission via blood HIV, HTLV-1, HBV, HCV, hepatitis delta virus,
cytomegalovirus
3. Genetic Prions, retroviruses
4. Maternal-neonatal transmission Rubella virus, cytomegalovirus, B19 virus,
echovirus, HSV, varicella-zoster virus, HIV
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4. What is the Viral Attachment Proteins (VAP) of Adenovirus?#1. VP1-VP2-VP3

complex #2. gp350 and gp220 #3. G protein gp #4. Fiber protein #5. E1-E2-E3 complex
gp
4. Fiber protein

1. Picornaviridae (Rhinovirus)
2. Herpesviridae (Epstein-Barr virus )
3. Rhabdoviridae (Rabies virus)
5. Togaviridae (Semliki Forest virus )

5. What is the mechanisms of viral cytopathogenesis from Adenovirus?#1. Inhibition

of cellular protein synthesis #2. Inhibition and degradation of cellular DNA #3.
Toxicity of virion components #4. Disruption of cytoskeleton #5. Syncytia formation
1. Poliovirus, herpes simplex virus (HSV), togaviruses, poxviruses
2. Herpesviruses
3. Adenovirus fibers, reovirus NSP4 protein
4. Nonenveloped viruses (accumulation), HSV
5. HSV, varicella-zoster virus, paramyxoviruses, human immunodeficiency virus

6. What is the name of inclusion bodies that create by Adenovirus?#1.

Intracytoplasmic#2. Intranuclear basophilic #3. Intracytoplasmic acidophilic #4.
Intranuclear#5. Perinuclear cytoplasmic acidophilic #
Negri bodies (intracytoplasmic) ------ Rabies virus
Intranuclear basophilic (Owl’s eye) -----Cytomegalovirus (enlarged cells),
Adenoviruses
Intracytoplasmic acidophilic ------- Poxviruses
Cowdry type A (intranuclear) --------- HSV, subacute sclerosing panencephalitis
(measles) virus
Perinuclear cytoplasmic acidophilic ---------- Reoviruses
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7. Below, which one is not Respiratory Diseases that mainly caused by Adenoviruses?
#1. Common Cold Like#2. Pharyngitis #3. Bronchiolitis #4. Pneumonia #5. Croup #
3. Bronchiolitis
RSV* (infants)
Metapneumovirus
Parainfluenza virus 3* (infants and children)
Parainfluenza viruses 1 and 2
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8. Below, which one is the most common of Respiratory Diseases that caused by
Adenoviruses?#1. Common Cold #2. Pharyngitis #3. Bronchiolitis #4. Pneumonia #5.
Croup #
2. Pharyngitis
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9. In Central Nervous System, Adenovirus Infections can causes what disease?#1.

Meningitis #2. Paralysis #3. Encephalitis #4. Postinfectious Encephalitis #5.
Tropical spastic paraparesis#
Meningitis

Paralysis Poliovirus Enteroviruses 70 and 71 Coxsackievirus A7

Encephalitis Herpes simplex virus 1* Varicella-zoster virus Arboencephalitis
viruses* Rabies virus Coxsackieviruses A and B Polioviruses
Postinfectious Encephalitis (Immune Mediated) Measles virus Mumps virus Rubella
virus Varicella-zoster virus Influenza viruses
Tropical spastic paraparesis ----- Human T-cell lymphotropic virus 1
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10. Which one below is organs and tissues that Adenoviruses as most causal agent
and also Adenovirus can infections?#1. Liver #2. Heart #3. Muscle #4. Glands #5.
Eye #6. Kidney#
5. Eye

Liver
Hepatitis A,* B,* C,* G, D, and E viruses Yellow fever virus Epstein-Barr virus
Hepatitis in the neonate or immunocompromised person: Cytomegalovirus Herpes
simplex virus Varicella-zoster virus Rubella virus (congenital rubella syndrome)
#2. Heart ----- Coxsackievirus B #3. Muscle ---- Coxsackievirus B (pleurodynia) #4.
Glands ----- Cytomegalovirus Mumps virus Coxsackievirus B
6. Kidney ------ Cytomegalovirus #
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11. Adenovirus is also being used in genetic therapies , except ?#1. for cystic
fibrosis #2. to express genes for other viruses#3. as a vaccine#4. as oncolytic
therapy#5. to deliver RNA for gene replacement and modification therapy
5. to deliver DNA (true) for gene replacement and modification therapy
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12. How many hours Adenovirus replication cycle takes?

approximately 32 to 36 hours

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13. Which statement is not true about adenovirus?#1. Naked icosadeltahedral

capsid#2. Virus encodes its own DNA polymerase#3. Human adenoviruses are grouped A
through J by DNA homologies and by serotype #4. Virus causes lytic, persistent,
and latent infections in humans#5. Some strains can immortalize certain animal
cells
3. A through G
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14. Limits viral cytopathologic effect. What is the gene protein have this
function?#1. E1A#2. E2#3. E3#4. E4 #5. VA RNAs
4. E4
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15. Binds cellular growth suppressor (p53) to promote cell growth and
transformation, Blocks apoptosis . WHAT IS THE GENE PROTEIN HAVE THIS FUNCTION?
E1B
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16. Activates viral gene transcription Binds cellular growth suppressor (p105RB) to
promote cell growth and transformation Deregulates cell growth Inhibits activation
of interferon response elements. WHAT IS THE GENE PROTEIN HAVE THIS FUNCTION?
E1A
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17. Activates some promoters Terminal protein on DNA DNA polymerase WHAT IS THE
GENE PROTEIN HAVE THIS FUNCTION?
E2
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18. Inhibits interferon response. What is the gene protein have this function?#1.
E1A#2. E2#3. E3#4. E4 #5. VA RNAs
5. VA RNAs
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19. The E1A protein is also an oncogene, and together with the E1B protein, it can
stimulate cell growth by binding to the cellular growth-suppressor proteins. What
spesific cellular growth-suppressor proteins that E1A protein binding?
p105RB (p105RB retinoblastoma gene product) (E1A)

p53 (E1B).

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20. The E1A protein is also an oncogene, and together with the E1B protein, it can
stimulate cell growth by binding to the cellular growth-suppressor proteins. What
spesific cellular growth-suppressor proteins that E1B protein binding?
p105RB (p105RB retinoblastoma gene product) (E1A) and p53 (E1B).

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21. Where replication of DNA Adenovirus occurs?

in the nucleus
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22. By what mediation that adenovirus DNA can replication ?

Is mediated by the viral-encoded DNA polymerase

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23. Where the capsid proteins of Adenoviruses is produced?
produced in the cytoplasm

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24. Adenoviruses are capable of causing lytic, latent and transforming infections.
What cells in our body that Adenoviruses can be latent?
lymphoid and adenoid cells
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25. Which statement is not true about adenovirus?#1. Fingers spread virus to eyes.
#2. Virus infects mucoepithelial cells in the respiratory tract#3. Virus persists
in neural tissue#4. Antibody is important for prophylaxis and resolution
3. Virus persists in neural tissue (lymphoid tissue e.g., tonsils, adenoids, Peyer
patches).
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26. A 7-year-old student develops sudden onset of red eyes, sore throat, and a
fever of 38.9° C (102° F). Several children in the local elementary school have
similar symptoms. What is clinical diagnosis?
Pharyngoconjunctival fever
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27. A baby girl, 6 months old has diarrhea and vomiting. Clinical diagnosis as
Gastroenteritis. Name the type of Adenovirus that caused this clinical
manifestastion.
Adenovirus type 40, 41
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28. A 7-year-old student develops sudden onset of red eyes, sore throat, and a
fever of 38.9° C (102° F). Several children in the local elementary school have
similar symptoms. Name the type of Adenovirus that caused this clinical
manifestastion.
Pharyngoconjunctival fever
Adenovirus type 1, 2, 3, 4, 5, 7
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29. An infant has diarrhea and is vomiting. Adenovirus sero-type 41 is identified

by polymerase chain reaction analysis of stool for epidemiologic reasons. What is
clinical diagnosis?##
Infantile Gastroenteritis
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30. Name the type of Adenovirus that caused this clinical manifestastion.
3,7
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31. Clinical diagnosis ?

Follicular conjunctivitis (pinkeye) , pharyngoconjuctival fever

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32. Acute respiratory disease. Name the type of adenovirus.

1, 2, 4, 5, 6, 7, 14, 21
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33. Pertussis-like syndrome . Name the type of adenovirus.

5
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34. Acute hemorrhagic cystitis.#Name the type of adenovirus.
11, 21
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35 Nephritis. Name the type of adenovirus.

11, 21
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36. Epidemic keratoconjunctivitis . Name the type of adenovirus.

8, 9, 11, 19, 35, 37
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37. Hepatitis . Name the type of adenovirus.

1-5, 7, 31
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38. Pneumonia. Name the type of adenovirus.

3, 4, 7, 14, 21, 30
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39. Meningoencephalitis. Name the type of adenovirus.

2, 7

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40. Gastroenteritis. Name the type of adenovirus.

40, 41
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41. Adenovirus infection can happen in military recruits. What associated disease?
#1. Acute respiratory disease #2. Pharyngoconjunctival fever #3. Acute hemorrhagic
cystitis/nephritis #4. Meningoencephalitis #
1. Acute respiratory disease
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42. Adenovirus infection can happen in any age of patient population. What
associated disease?#1. Acute respiratory disease #2. Pharyngoconjunctival fever #3.
Acute hemorrhagic cystitis/nephritis #4. Epidemic keratoconjunctivitis ##
4. Epidemic keratoconjunctivitis ##
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43. Adenovirus infection can happen in immunocompromised patients. What is disease

that not associated below?#1. Gastroenteritis #2. Hepatitis #3.
Pharyngoconjunctival fever #4. Meningoencephalitis #5. Pneumonia
3. Pharyngoconjunctival fever
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44. Which adenovirus types are most likely to cause acute respiratory distress
syndrome?

The most likely types are serotypes 4 and 7 but could also be 1, 2, 3, 5, 7, and
14.
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45. What is type of adenovirus that associated with obesity?

Adenovirus (type 36) is also associated with obesity.
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46. Adenovirus causes pharyngitis, which is often accompanied by conjunctivitis
(pharyngoconjunctival fever). Pharyngitis alone occurs in young children,
particularly those younger than 3 years, and may mimic what infection?
Streptococcal pharyngitis infection.
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47. Which statement is true about adenovirus?#1. Capsid virus is sensitive to

inactivation by gastrointestinal tract, drying, and detergents. #2. Transmission
also can happen via blood #3. High risk in people who live in crowded areas#4.
Virus occur in certai area not worldwide#5. There is seasonal incidence.
3. High risk in people who live in crowded areas
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48. What are laboratory diagnosis that we can be use to detect, type, and group the
adenovirus in clinical samples and tissue cultures?
Immunoassays (e.g., fluorescent antibody and enzyme-linked immunosorbent assay) and
genome assays (e.g., different variations of polymerase chain reaction [PCR] and
DNA probe analysis)
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49. There is no approved treatment for adenovirus infection, but there are
antibiotic that have been used to treat adenovirus-infected immunosuppressed
individuals. What antivirals?
cidofovir and ribavirin
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50. What can be use to prevent infections with adenovirus types 4 and 7 in military
recruits but are not used in civilian populations?
Live oral vaccines
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