PAPILLOMAVIRUS AND POLYOMAVIRUS##BY : RAJA PARDOMUAN HARAHAP

Formerly, what was the family where Polyomaviridae and Papillomaviridae classified?
1
Papovaviridae, papovavirus family

2
Which virus generally causes warts and cervical carcinoma?
Human papillomavirus

3
Name two members of Polyomaviridae family.
JC virus = Jamestown Canyon virus =John Cunningham virus
BK virus

Which member of the Polyomaviridae that associated with renal disease?

4
BK virus (BKV)

Which member of the Polyomaviridae that associated with progressive multifocal

leukoencephalopathy (PML)?
5
JC virus (JCV) = John Cunningham virus = Jamestown Canyon virus

What is the prototype of the polyomavirus?

6
Simian virus 40 (SV 40)

What is the genome component of both papillomavirus and polyomavirus?.

7
dsDNA (Baltimore classification group I)

8
Which member of papovavirus family that has circular genome?
Human papillomavirus

What is the morphological structure of both papillomavirus and polyomavirus?

9
Nonenveloped, icosahedral capsid virus

The promotion of the cell growth facilitates lytic viral replication in permissive
cell type. What is the infection form from that statement?
10
Lytic infection

The promotion of cell growth facilitates oncogenically transformation of a cell

that is nonpermissive. What is the infection form from that statement?
11
Transforming/immortalizing infection

What member of the Polyomaviridae have been studied extensively as model oncogenic
virus and is prototype of that family?
12
Simian virus 40 (SV40)

On the basis of susceptible tissue, what are the group classifications of human
papillomavirus (HPV)?
13
Mucosal HPV and cutaneous HPV
On the basis of the susceptible tissue, which group of HPV associated with cervical
cancer?
14
Mucosal HPV

15
How many maximum number of early genes that HPV DNA encodes?
7-8 early genes (E1 – E8), so the maximum number is 8

16
How many late/structural genes that HPV DNA encodes?
L1 and L2, so total = 2

17
Name two type of cells become location of replicating cycle of human
papillomavirus.
Keratinocyte, epithelial cell of the skin and mucosa/mucosal cells

Which layer of the skin may be accessed by the human papillomavirus through breaks
in the skin?
18
Basal cell layer/basal keratinocytes layer/stratum basale

What is the viral attachment protein (VAP) of human papillomavirus (HPV)?

19
L1

20
Name two receptors of human papillomavirus (HPV).
Heparin proteoglycans, integrin alpha-6

21
Which is not the form of infection caused by papovavirus family?##1. Lytic form#2.
Latent form#3. Chronic form#4. Transforming form#5. Lysogenic form
5

22
Which component of human papillomavirus (HPV) that stimulates cell growth, then
facilitates replication of the viral genome by the host cell DNA polymerase when
the cell divide?##1. Early gene#2. Late gene#3. Structural gene#4. Long control
region (LCR)
1

23
Which layer of the skin becomes thicken because of HPV-induced increase in cell
number?##1. Basal and dermis layer#2. Stratum granulosum and basal layer#3. Str.
granulosum and str. spinosum#4. Basal keratinocytes and prickle cell layer
4

24
Which component of human papillomavirus (HPV) DNA that encodes the structural
proteins expressed only in the terminally differentiated upper layer, and the virus
assembles in the nucleus?##1. Early gene#2. Late gene#3. Long control region
(LCR)#4. Epidermal growth factor (EGF)
2 = Late gene = structural gene

25
Where is the virus shed as the infected skin cell matures?##1. Skin capillaries#2.
Dead cells of the upper layer#3. Dead cells of the lower layer#4. Langerhans skin
cells
2

26
What disease associated with human papillomavirus (HPV) infection and replication
in the squamous epithelium of skin?##1. Genital papilloma#2. Oral papilloma#3.
Genital papilloma#4. Warts
4

27
Which is not the disease associated with human papillomavirus (HPV) infection and
replication in the mucous membranes?##1. Genital papilloma#2. Oral papilloma#3.
Genital papilloma#4. Warts
4

28
The wart develops because of virus stimulation of cell growth and thickening of
skin layer. Which is not the related skin layer?##1. Prickle layers/stratum
spinosum#2. Stratum granulosum#3. Keratinized stratum corneum#4. Basal layer of the
skin
3

29
Which condition is not caused by high-risk HPV types?##1. Conjunctival papilloma#2.
Cervical carcinoma#3. Penile cancer#4. Oropharyngeal cancer#5. esophageal cancer#6.
Anal cancer
1

30
Which is not the component of human papillomavirus (HPV) DNA type 16, 18 identified
as oncogenes?##1. E1#2. E5#3. E6#4. E7
1

Which gene of the human papillomavirus (HPV) DNA enhances cell growth by
stabilizing the epidermal growth factor (EGF) receptor to make the cell more
sensitive to growth signals?##1. E1#2. E5#3. E6#4. E7
31
2

32
Which gene of the human papillomavirus (HPV) DNA that binds the p53 protein and
targets it for degradation?##1. E1#2. E5#3. E6#4. E7
3

33
Which gene of the human papillomavirus (HPV) DNA that binds and inactivates p105
retinoblastoma gene products?##1. E1#2. E5#3. E6#4. E7
4

34
Which gene of the human papillomavirus (HPV) DNA that activates telomerase and
suppresses apoptosis?##1. E1#2. E5#3. E6#4. E7
3

35
Which is less commonly transmission of human papillomavirus (HPV) infection?##1.
Direct contact through small breaks in the skin or mucosa#2. Through blood
transfusion#3. During sexual intercourse#4. While an infant is passing through an
infected birth canal
2

36
What is not the major risk factor for infection and progression to cancer caused by
HPV?##1. Smoking#2. Family history with cervical cancer#3. Drug abuser#4.
Immunosuppression#5. Multiple sexual partners
3

37
What is not cutaneous group or cutaneous syndrome of HPV infection?##1. Plantar
wart#2. Common wart#3. Flat wart#4. Condyloma acuminatum#5. Epidermodysplasia
verruciformis
4

38
Which HPV type that causes common wart?##1. 6, 11#2. 1#3. 2, 4#4.
3, 10
3

39
Which HPV type that causes flat wart?##1. 6, 11#2. 1#3. 2, 4#4. 3,
10
4

40
Which disease/condition associated with HPV-16, -18?##1. Cervical intraepithelial
neoplasia#2. Condyloma acuminatum#3. Conjunctival papilloma#4. Common wart
1

What is the characteristic name of enlarged keratinocytes with clear halos around
shrunken nuclei caused by human papillomavirus (HPV) infection?
41
Koilocytes

Which host immune response is responsible for control and resolution of HPV
infections?
42
Innate immune response, cell-mediated immunity

Where could we find the high levels of antigen expression of human papillomavirus
(HPV)?
43
Near dead skin cells or in the terminally differentiated skin cells

What viral component of the human papillomavirus (HPV) becomes target of

neutralizing antibodies?
44
L1

45
What neutralizing antibodies secreted into the vagina and can protect against human
papillomavirus (HPV) infection?
IgG

Almost all cervical carcinomas contain integrated HPV DNA, with 70% from which
types?
46
HPV-16, HPV-18
47
Mention four types of HPV that are high-risk HPV for African American women.
HPV 33, 35, 58, 68

Mention low-risk HPV types for cervical carcinoma but cause condyloma acuminatum,
oral, and laryngeal papillomas.
48
HPV-6, HPV-11

10% of women infected with the high-risk HPV types will develop what pathological
condition?
49
Cervical dysplasia, a precancerous state

Name three diseases/conditions included as benign head and neck tumors.

50
Conjunctival, oral, laryngeal papillomas

51
Name four diseases/conditions included as skin warts.
Plantar wart, common wart, flat wart, epidermodysplasia verruciformis

52
Name two diseases/conditions included as anogenital warts.
Condyloma acuminatum, cervical intraepithelial neoplasia (cancer)

Mention HPV types that cause laryngeal, oral papillomas, and condyloma acuminatum.
53
HPV-6, HPV-11

54
Mention HPV type that causes conjunctival papilloma.
HPV-11

55
Mention common HPV type that causes plantar wart.
1

56
Which is not the less prevalent human polyomaviruses?##1. KI polyomavirus#2. Simian
virus 40#3. Merkel cell polyomavirus#4. WU polyomavirus
2

57
Which is not the part of the genome of BKV, JCV, and SV40?##1. Structural gene#2.
Early region#3. Late region#4. Noncoding region
1

58
Which is not the early region of the genome of polyomaviruses encodes?##1. Large T
antigen#2. T’ antigen#3. Small t antigen#4. VP1 protein
4

59
Which is not the late region of the genome of polyomaviruses encodes?##1. VP1
protein#2. T’ antigen#3. VP2 protein#4. VP3 protein
2
60
Which is not the receptor of human papovavirus family?##1. Integrin alpha-6#2.
Heparin proteoglycans#3. Neural cell adhesion molecule#4. Sialylated carbohydrates
receptor#5. Serotonin receptor
3

61
What is the viral attachment protein (VAP) of polyomavirus?##1. Large T antigen#2.
Small t antigen#3. VP1 protein#4. VP2 protein#5. VP3 protein
3

62
JCV and BKV are human viruses that probably enter the respiratory tract or tonsils,
after which they infect lymphocytes and then the kidney with a minimal
cytopathologic effect. BKV establishes latent infection in what cell/tissue/organ?
##1. B lymphocytes#2. Monocyte-lineage cells#3. Oligodendrocytes#4. Astrocytes#5.
Kidney
5

63
Replication of JCV and BKV is blocked in immunocompetent persons. In T-cell-
deficient patients (e.g., AIDS), reactivation of the latent virus in the kidney
leads to viral shedding in which clinical specimen?##1. Capillary blood#2. Pus
through the urethra#3. Clinical tonsillar exudates#4. Urine
4

64
What is the drug for both HPV and polyomaviruses infections?##1. Acyclovir#2.
Ganciclovir#3. Zidovudine for AIDS patiens#4. Cidofovir
4

65
What is the mechanism of action of cidofovir?
Inhibits host cell DNA polymerase

66
What is the mechanism of action of acyclovir?
Inhibits viral DNA polymerase

67
Mention HPV types that cause that condition.
HPV-2, HPV-4 = COMMON WART

68
What is the name of process of vacuolated cytoplasm showed by this picture?
Koilocytosis

69
What is the name of this procedure to gain this appearance and what is the specific
causative agent?
Papanicolaou pap smear procedure, HPV-16, HPV-18

70
Progressive multifocal leukoencephalopathy caused by JCV is a subacute
demyelinating disease that occurs in immunocompromised patients such as AIDS. From
that statement, what is the infected cell lead to that condition?##1. Astrocyte#2.
Oligodendrocyte#3. B lymphocyte#4. Monocyte-lineage cell
2
71
Name two receptors for John Cunningham virus.
Sialylated carbohydrates, serotonin receptors

72
Name the cell where the infection of JC virus generally occurs.
Glial cells

73
Name types of human cells where JC virus causes latent and chronic infections.
Monocyte, Lymphocyte, Oligodendrocyte, Astrocyte

74
What is the antigen encoded by early region of simian virus 40 that binds to DNA
and controls early and late gene transcription, as well as viral DNA replication?
Large T antigen

75
What is the antigen encoded by early region of polyomaviruses that inactivates p53
and p105RB?
Large T antigen