RICKETTSIACEAE, ANAPLASMATACEAE, COXIELLACEAE##BY : RAJA PARDOMUAN HARAHAP

Name two genera of family Rickettsiaceae.

1
Rickettsia, Orientia

2
Name two genera of family Anaplasmataceae.
Anaplasma, Ehrlichia

3
Name a genus of family Coxiellaceae.
Coxiella

Name two groups of genus Rickettsia.

4
Spotted fever and typhus groups

What is the etiologic agent of Rocky Mountain spotted fever?

5
Rickettsia rickettsii

Rickettsialpox is associated with which bacterial species?

6
Rickettsia akari

Name the bacterial species classified in spotted fever group.

7
Rickettsia rickettsii, Rickettsia akari

Which rickettsial group in which the Rickettsia prowazekii classified?

8
Typhus group

What is the etiologic agent of scrub typhus?

9
Orientia tsutsugamushi

Which bacterial species classified in typhus group?

10
Rickettsia prowazekii, Rickettsia typhi

Orientia tsutsugamushi is associated with what disease?

11
Scrub typhus

Mite disease is associated with which bacterial species?

12
Orientia tsutsugamushi

Which bacterial genus is associated with intracytoplasmic inclusions?

13
Anaplasma

What is the rickettsial cell wall structure has weak endotoxin activity?
14
Lipopolysaccharide

Rickettsia and Orientia stains poorly with Gram stain, so both are seen best with
special stains. Name two of those stains.
15
GIEMSA, GIMENEZ stains

16
Rickettsia and Orientia are strict intracellular parasites. Where within the
infected cells are they growing and replicating freely?
CYTOPLASM

17
After engulfment by phagocyte, Rickettsia and Orientia degrade the phagosome
membrane by producing which enzyme?
Phospholipase

Orientia and the spotted group of Rickettsia grow in the cytoplasm and nucleus of
infected cells and are continually released from cells through what formation?
18
Long cytoplasmic projections

In contrast to the spotted fever group, what is the effect of accumulation of the
typhus group of Rickettsia in the cell cytoplasm?
19
The typhus group of Rickettsia accumulates in the cell cytoplasm until the cell
membrane lyse, signalling cell death and bacterial release.

20
What is the intracellular motility feature of spotted fever group of Rickettsia?
Spotted fever group is able to polymerize host cell actin, whereas the typhus group
lacks the required gene.

21
Which disease that has macular centripetal rash?##1. Rocky Mountain spotted
fever#2. Rickettsialpox#3. Epidemic typhus#4. Endemic typhus
1

22
Which disease that has papulovesicular generalized rash?##1. Rocky Mountain spotted
fever#2. Rickettsialpox#3. Epidemic typhus#4. Endemic typhus
2

23
Which disease that has maculopapular rash on trunk?##1. Rocky Mountain spotted
fever#2. Rickettsialpox#3. Epidemic typhus#4. Endemic typhus
4

24
Which disease that has macular centrifugal rash?##1. Rocky Mountain spotted
fever#2. Rickettsialpox#3. Epidemic typhus#4. Endemic typhus
3

25
Which disease that has human body louse as the vector?##1. Rocky Mountain spotted
fever#2. Rickettsialpox#3. Epidemic typhus#4. Endemic typhus
3 = Epidemic typhus = Louse-borne typhus

26
Which disease that has cat and rat fleas as the vector?##1. Rocky Mountain spotted
fever#2. Rickettsialpox#3. Epidemic typhus#4. Endemic typhus
4
27
Which bacterial species that has blacklegged tick as the vector of disease?##1.
Anaplasma phagocytophilum#2. Rickettsia prowazekii#3. Coxiella burnetii#4.
Ehrlichia chaffeensis
1.
philum pakai i bukan y
Anaplasma phagocytophilum menyebabkan human granulocytic anaplasmosis

28
Which bacterial species that causes query fever?##1. Anaplasma phagocytophilum#2.
Rickettsia prowazekii#3. Coxiella burnetii#4. Ehrlichia chaffeensis
3

29
Which bacterial species that causes endemic typhus?##1. Rickettsia typhi#2.
Rickettsia prowazekii#3. Orientia tsutsugamushi#4. Rickettsia akari
1

30
Relapse disease by recrudescent typhus is caused by which bacterial species?##1.
Rickettsia typhi#2. Rickettsia prowazekii#3. Orientia tsutsugamushi#4. Rickettsia
akari
2

Which species becomes the vector of scrub typhus?##1. Orientia tsutsugamushi#2.

Leptotrombidium sp.#3. Rhipicephalus sanguineus#4. Dermacentor andersoni
31
2
1 = causative agent of scrub typhus
3 = brown dog tick
4 = wood tick

32
Which is not species becomes the vector of Rocky Mountain spotted fever?##1.
Dermacentor variabilis#2. Leptotrombidium sp.#3. Rhipicephalus sanguineus#4.
Dermacentor andersoni
2
1 = American dog tick
3 = brown dog tick
4 = wood tick

33
Which is the species becomes the vector of spotted fever in Arizona?##1.
Dermacentor variabilis#2. Leptotrombidium sp.#3. Rhipicephalus sanguineus#4.
Dermacentor andersoni
3

34
Which is the species becomes the vector of spotted fever in Rocky Mountain States
and southwestern Canada?##1. Dermacentor variabilis#2. Leptotrombidium sp.#3.
Rhipicephalus sanguineus#4. Dermacentor andersoni
4

35
Ehrlichia ewingii and Ehrlichia chaffeensis shared the same vector. What is it?##1.
Trombiculidae mites#2. Lone Star ticks#3. Blacklegged ticks#4. Human body louse
2
1 = vector for Orientia tsutsugamushi
3 = vector for Anaplasma phagocytophilum
4 = vector for Rickettsia prowazekii as epidemic (louse-borne) typhus vector
Lone Star and blacklegged ticks are soft ticks

36
What is the etiologic agent of sporadic typhus?##1. Rickettsia prowazekii#2.
Rickettsia typhi#3. Rickettsia rickettsii#4. Rickettsia akari
1
2 = Endemic (murine) typhus
3 = Rocky Mountain spotted fever
4 = Rickettsialpox disease

37
What is the reservoir of sporadic typhus?##1. Humans#2. Chiggers (larval mites)#3.
Raccoons#4. Squirrels and squirrels fleas#5. Cat
4

38
Which disease that is acquired most commonly by inhalation of causative agent and
has ticks incidental vector?##1. Sporadic typhus#2. Scrub typhus#3. Query fever#4.
Human granulocytic anaplasmosis
3

39
Hard ticks are vector of what disease?##1. Rickettsialpox#2. Rocky Mountain spotted
fever#3. Scrub typhus#4. Human granulocytic anaplasmosis
2
1 = Rickettsia akari = mites
3 = Orientia tsutsugamushi = Leptotrombidium deliense chiggers/mites
4 = Anaplasma phagocytophilum = BLACKLEGGED TICK (soft tick)

40
What disease has maculopapular centrifugal rash ?##1. Rickettsialpox#2. Rocky
Mountain spotted fever#3. Scrub typhus#4. Endemic typhus#5. Epidemic typhus
3
1 = papulovesicular generalized rash
2 = macular centripetal rash
4 = maculopapular rash on trunk
5 = macular centrifugal rash

What is the etiologic agent of human monocytic ehrlichiosis?

41
Ehrlichia chaffeensis

What is the etiologic agent of human granulocytic ehrlichiosis?

42
Ehrlichia ewingii

Name this staining.

43
GIMENEZ STAINING

What is the genus of the causative organism?

44
Rickettsia

In contrast with Rickettsia and Orientia, Ehrlichia and Anaplasma remain in the
phagocytic vacuole after entry into the host cell. What mechanism is prevented
because expression of appropriate receptors on the phagocytic vacuole is
interrupted?
45
Fusion with lysosomes

Q fever is associated with which bacterial species?

46
Coxiella burnetii – Q (Query) fever

47
Name two structural forms of Coxiella burnetii.
Small cell variants that are resistant to environmental stress (e.g., heat,
desiccation, chemical agents) and large cell variants that are the metabolically
active form.

Which structural form of Coxiella burnetii is analog with reticulate body of

Chlamydia trachomatis?
48
Large cell variants, both large cell variant and reticulate body are metabolically
active.

Which structural form of Coxiella burnetii that is resistant to dessication and

heat?
49
Small cell variant

What is the etiologic agent of canine granulocytic anaplasmosis?

50
Anaplasma phagocytophilum

Which transmission does not occur in Ehrlichia and Anaplasma, in contrast to

Rickettsia and Orientia?
51
Transovarian transmission

What is the accidental host of Ehrlichia and Anaplasma?

52
Human

What is the treatment of chronic Q fever?

53
Doxycycline + alkalinizing agent of hydroxychloroquine

What is alternative drug for query fever but contraindicated with pregnant women?
54
Fluoroquinolones (ofloxacin, pefloxacin)

What is cycle of the Coxiella burnetii becomes target of inacticvated whole-cell

vaccine?
55
Fluoroquinolones (ofloxacin, pefloxacin)

56
Which species is the vector of Ehrlichia chaffeensis?##1. Dermacentor andersoni#2.
Amblyomma americanum#3. Rhipicephalus sanguineus#4. Dermacentor variabilis#5.
Ixodes scapularis
2

57
Which is the reservoir of Ehrlichia chaffensis?##1. White-tailed deer#2. White-
footed mouse#3. Chipmunks#4. Voles#5. Ixodes tick
1

58
Which is not the reservoir of Anaplasma phagocytophilum?##1. White-tailed deer#2.
White-footed mouse#3. Chipmunks#4. Voles#5. Ixodes tick
1

59
In which structural component/phase, Coxiella burnetii has intact
lipopolysaccharide?##1. Phase I#2. Phase II#3. Small cell variant#4. Large cell
variant#5. Reticulate body
1

60
In which structural component/phase, Coxiella burnetii has lost its outermost O-
antigen sugars component due to mutation occur in the LPS genes?##1. Phase I#2.
Phase II#3. Small cell variant#4. Large cell variant#5. Reticulate body
2

61
What is the drug of choice of ehrlichiosis and anaplasmosis?##1. Erithromycin#2.
Azithromycin#3. Penicillin G#4. Doxycycline#5. Fluoroquinolones
4

62
E. chaffeensis and E. ewingii are closely related and cannot be differentiated by
serology. The specificity of the serology tests is NOT compromised by cross-
reactions with what disease?##1. Rocky Mountain spotted fever#2. Q fever#3. Lyme
disease#4. Brucellosis#5. Anaplasmosis
5

63
Which is not clinical finding of human monocytic ehrlichiosis?##1.
Thrombocytopenia#2. Leukopenia#3. Elevated serum transaminases#4. Hemoglobinuria
4

64
Which is not clinical finding of human granulocytic ehrlichiosis?##1.
Thrombocytopenia#2. Leukopenia#3. Elevated serum transaminases#4. Hemoglobinuria
4

65
Which is the most common laboratory workup for ehrlichiosis?##1. Microscopy#2.
Nucleic acid amplification test#3. ELISA#4. Antigen detection test
2

66
What is the name of this appearance?
Multiple morulae in Ehrlichia canis/Ehrlichia chaffeensis

67
Human infection caused by Coxiella burnetii occur after what condition?##1.
Inoculation through the trauma#2. Ingestion of unpasteurized milk#3. Through the
tick bites#4. Direct contact through the skin#5. Through the lice bites
2

68
Serologic test for Coxiella burnetii must include the other bacterial genus because
both have similar disease with cross-reaction?#1. Brucella#2. Pseudomonas#3.
Wolbachia#4. Streptobacillus#5. Bartonella
5

69
In chronic Q fever, antibodies are developed primarily against what structural
form/phase of Coxiella burnetii?##1. Phase I#2. Phase II#3. Reticulate body#4.
Elementary body#5. Small cell variant#6. Large cell variant
1

70
In acute Q fever, IgM and IgG antibodies are developed primarily against what
structural form/phase of Coxiella burnetii?##1. Phase I#2. Phase II#3. Reticulate
body#4. Elementary body#5. Small cell variant#6. Large cell variant
2