i (a. Haemadsorption The surfaces of Some virus ) infected cells are altered in such a way that eryth. rocytes adhere to them. This adherence of red cells to the virus infeted cells is called haemadsorption, The viruses which are capable of inducing haemadsorption (HAD) are influenza, parain- fluenza, mumps and measles. Most of these viruses are also capable of haemagglutination. A modifi- cation of haemadsorption test can detect both haemadsorption and haemagglutination.enna np nn nn nn np Ui, Technique 1. Remove the culture fluid from the celj culture tube containing infected cells. 2. Add 2ml of 0.08% guinea pig erythrocyte suspension to the culture tube. 3. Repeat the same procedure with an uninfected cell line of the same type. 4. Place the tubes in a slanted position so that the erythrocyte suspension covers the monolayer.5. Incubate at 4°C for 30 minutes. 6. Examine microscopically for the adherence 10. of erythrocytes to the infected cells. The haemadsorption appears as rosettes of erythrocytes covering the cell surface. To observe haemagglutination, allow the tubes to stand vertically overnight at room temperature. Observe the red cells at the bottom of the tube. If the red cells show a shield pattern, the culture is positive for haemagglutination. If the red cells settle to form a button, the haemagglutination test is negative. ti tl t