RESULT SITI AISYAH

Picture 1.1 Front view Picture 1.2 Reverse view

This is the result for the agar media after one week. Through the images, this experiment was
unsuccessful. There was no fungi growing on the media. There are several factors that cause
this to happen:
1. Over-burnt leaves
2. The leaves was shaken for more than 1 minute in the alcohol solution.

RESULT BASED ON OTHER AGAR MEDIA

Picture 1.3 Front view Picture 1.4 Front view

This agar media shows the fungi is growth on the media within one week. There are three types
fungi that growth on the media.
Stereo microscope

The colour is dark brown and

cottony.

PICTURE A
-Zygomycetes

The colour is white and cottony

PICTURE B
-Dimorphic Fungi

The colour is white grey on the

top surface and grey to black
on the revese surface.

PICTURE C
-Zygomycetes
DISCUSSION

Microbe contamination refers to the presence of unwanted microorganisms in an

environment or sample, potentially affecting scientific, industrial, or medical studies,
originating from various sources. Microbes can contaminate an environment through air,
surfaces, and humans and animals. Air can spread microbe particles, especially in unclean
environments like dusty areas or uncontrolled settings. Surfaces can sustain bacteria, fungi, or
viruses, if not properly cleaned or sterilized. Humans and animals can also act as carriers of
microbes, contaminating the environment through touch, aerosols, or microbial transmission.(
Sinuraya, Rodiansah, & Sofia Hana, 2022)
Traditionally, the colony measurement process is carried out directly by measuring
the radius of the colony area on the plate using digital rulers or calipers. As pointed out in Kai
et al. (2019) this procedure has two limitations: (i) the definition of colony limits on the plate
depends on the judgment of the professional performing the measurement; and (ii) radial
growth may not be uniform in all directions, leading to errors in colony area
calculation.Fungal colonies from the fungal isolation process is the fungal colonies obtained
from a fungal isolation process on an agar plate will exhibit a variety of characteristics that
can help identify the fungal species. Upon initial incubation of the inoculated agar plate,
small fungal colonies will begin to emerge. These initial colonies will continue to grow and
change in morphology over time.The shape, size, texture, and color of the fungal colonies can
provide important clues as to the fungal species. Colony shape may be circular, filamentous,
irregular, or rhizoid. Colony size can range from pinpoint to several centimeters in diameter
depending on the growth rate of the fungus. Colony texture may be described as powdery,
velvety, cottony, or leathery. Colony color can vary widely from white to gray to brown to
black to various hues, depending on the pigments produced by the fungus.The presence of
aerial hyphae, sporulation, and exudates can also aid in identification. Aerial hyphae refer to
hyphae that grow up above the agar surface, giving the colony a fluffy or fuzzy appearance.
Some fungi will produce spores on the colony surface, appearing as powdery dots or patches
of color. Exudates such as droplets of liquid or crystals may be present and have a
characteristic color and morphology.Microscopic examination of the fungal structures such as
hyphae, spores, and reproductive structures can provide definitive identification of the fungus
to the genus and species level. However, examination of the macroscopic colony
characteristics as described above during initial growth on an agar plate provides valuable
preliminary clues to narrow the possibilities and guide further investigation. A thorough
documentation of colony observations over time, along with photographs of the colonies, can
aid in the fungal identification process.
REFFERENS

Kai et al. (2019) .Investigating a non-destructive alternative for a preliminary evaluation of

fungal growth in solid state fermentations Journal of Microbiological Methods (2019)

https://www.sciencedirect.com/science/article/abs/pii/S095741742301374X

Sinuraya, M., Rodiansah, A., & Sofia Hana, D. (2022, June 15). Isothiazolone Reduced
Microbe Contamination of North Sumatra Local Garlic (Allium sativum L.) in vitro. Asian
Journal of Plant Sciences, 21(3), 416–422. https://scialert.net/abstract/?
doi=ajps.2022.416.422