Name: Nischit Kumar K

ID: 2023B3PS0813G
Grp No: 6
Experiment: 12
Section: P13

Pepstat (P53 Human Gene)

PEPSTATS of P53_HUMAN from 1 to 393

Molecular weight = 43653.18 Residues = 393

Average Residue Weight = 111.077 Charge = 2.0
Isoelectric Point = 6.7615
A280 Molar Extinction Coefficients = 35410 (reduced) 36035 (cystine
bridges)
A280 Extinction Coefficients 1mg/ml = 0.811 (reduced) 0.825 (cystine
bridges)
Improbability of expression in inclusion bodies = 0.865

Residue Number Mole% DayhoffStat

A = Ala 24 6.107 0.710
B = Asx 0 0.000 0.000
C = Cys 10 2.545 0.877
D = Asp 20 5.089 0.925
E = Glu 30 7.634 1.272
F = Phe 11 2.799 0.777
G = Gly 23 5.852 0.697
H = His 12 3.053 1.527
I = Ile 8 2.036 0.452
J = --- 0 0.000 0.000
K = Lys 20 5.089 0.771
L = Leu 32 8.142 1.100
M = Met 12 3.053 1.796
N = Asn 14 3.562 0.828
O = --- 0 0.000 0.000
P = Pro 45 11.450 2.202
Q = Gln 15 3.817 0.979
R = Arg 26 6.616 1.350
S = Ser 38 9.669 1.381
T = Thr 22 5.598 0.918
U = --- 0 0.000 0.000
V = Val 18 4.580 0.694
W = Trp 4 1.018 0.783
X = Xaa 0 0.000 0.000
Y = Tyr 9 2.290 0.674
Z = Glx 0 0.000 0.000
Property Residues Number Mole%
Tiny (A+C+G+S+T) 117 29.771
Small (A+B+C+D+G+N+P+S+T+V) 214 54.453
Aliphatic (A+I+L+V) 82 20.865
Aromatic (F+H+W+Y) 36 9.160
Non-polar (A+C+F+G+I+L+M+P+V+W+Y) 196 49.873
Polar (D+E+H+K+N+Q+R+S+T+Z) 197 50.127
Charged (B+D+E+H+K+R+Z) 108 27.481
Basic (H+K+R) 58 14.758
Acidic (B+D+E+Z) 50 12.723
Pepinfo (P53 Human Gene)
Local Alignment (Water) [P53 Human Gene and P53 Monkey Gene]
########################################
# Program: water
# Rundate: Fri 1 Sep 2023 11:31:32
# Commandline: water
# -auto
# -stdout
# -asequence emboss_water-I20230901-113128-0570-42447277-p1m.asequence
# -bsequence emboss_water-I20230901-113128-0570-42447277-p1m.bsequence
# -datafile EBLOSUM62
# -gapopen 10.0
# -gapextend 0.5
# -aformat3 pair
# -sprotein1
# -sprotein2
# Align_format: pair
# Report_file: stdout
########################################

#=======================================
#
# Aligned_sequences: 2
# 1: P53_HUMAN
# 2: P53_CHLAE
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 393
# Identity: 376/393 (95.7%)
# Similarity: 381/393 (96.9%)
# Gaps: 0/393 ( 0.0%)
# Score: 2032.0
#
#
#=======================================

P53_HUMAN 1 MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDI 50
|||||||||:|||||||||||||||||||||||||||||:|||||||||:
P53_CHLAE 1 MEEPQSDPSIEPPLSQETFSDLWKLLPENNVLSPLPSQAVDDLMLSPDDL 50

P53_HUMAN 51 EQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQ 100

.||.||||||||||||.||||.:||.||||||||||||||||||||||||
P53_CHLAE 51 AQWLTEDPGPDEAPRMSEAAPHMAPTPAAPTPAAPAPAPSWPLSSSVPSQ 100

P53_HUMAN 101 KTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDST 150

|||.||||||||||||||||||||||||.|||||||||||||||||||||
P53_CHLAE 101 KTYHGSYGFRLGFLHSGTAKSVTCTYSPDLNKMFCQLAKTCPVQLWVDST 150

P53_HUMAN 151 PPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGN 200

||||:|||||||||||||||||||||||||||||||||||||||||||||
P53_CHLAE 151 PPPGSRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGN 200

P53_HUMAN 201 LRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRP 250

|||||.||||||||||||||||||||||||||||||||||||||||||||
P53_CHLAE 201 LRVEYSDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRP 250

P53_HUMAN 251 ILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELP 300

||||||||||||||||||||||||||||||||||||||.||||||.||||
P53_CHLAE 251 ILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENFRKKGEPCHELP 300

P53_HUMAN 301 PGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALEL 350

||||||||||||||||||||||||||||||||||||||||||||||||||
P53_CHLAE 301 PGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALEL 350

P53_HUMAN 351 KDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD 393

|||||||||.||||||||||||||||||||||.||||||||||
P53_CHLAE 351 KDAQAGKEPAGSRAHSSHLKSKKGQSTSRHKKFMFKTEGPDSD 393

#---------------------------------------
#---------------------------------------

Global Alignment (Needleman) [P53 Human Gene and P53 Monkey Gene]
########################################
# Program: needle
# Rundate: Fri 1 Sep 2023 11:33:39
# Commandline: needle
# -auto
# -stdout
# -asequence emboss_needle-I20230901-113333-0736-70518898-p1m.asequence
# -bsequence emboss_needle-I20230901-113333-0736-70518898-p1m.bsequence
# -datafile EBLOSUM62
# -gapopen 10.0
# -gapextend 0.5
# -endopen 10.0
# -endextend 0.5
# -aformat3 pair
# -sprotein1
# -sprotein2
# Align_format: pair
# Report_file: stdout
########################################

#=======================================
#
# Aligned_sequences: 2
# 1: P53_HUMAN
# 2: P53_CHLAE
# Matrix: EBLOSUM62
# Gap_penalty: 10.0
# Extend_penalty: 0.5
#
# Length: 393
# Identity: 376/393 (95.7%)
# Similarity: 381/393 (96.9%)
# Gaps: 0/393 ( 0.0%)
# Score: 2032.0
#
#
#=======================================

P53_HUMAN 1 MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDI 50
|||||||||:|||||||||||||||||||||||||||||:|||||||||:
P53_CHLAE 1 MEEPQSDPSIEPPLSQETFSDLWKLLPENNVLSPLPSQAVDDLMLSPDDL 50

P53_HUMAN 51 EQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQ 100

.||.||||||||||||.||||.:||.||||||||||||||||||||||||
P53_CHLAE 51 AQWLTEDPGPDEAPRMSEAAPHMAPTPAAPTPAAPAPAPSWPLSSSVPSQ 100

P53_HUMAN 101 KTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDST 150

|||.||||||||||||||||||||||||.|||||||||||||||||||||
P53_CHLAE 101 KTYHGSYGFRLGFLHSGTAKSVTCTYSPDLNKMFCQLAKTCPVQLWVDST 150

P53_HUMAN 151 PPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGN 200

||||:|||||||||||||||||||||||||||||||||||||||||||||
P53_CHLAE 151 PPPGSRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGN 200

P53_HUMAN 201 LRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRP 250

|||||.||||||||||||||||||||||||||||||||||||||||||||
P53_CHLAE 201 LRVEYSDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRP 250

P53_HUMAN 251 ILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELP 300

||||||||||||||||||||||||||||||||||||||.||||||.||||
P53_CHLAE 251 ILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENFRKKGEPCHELP 300

P53_HUMAN 301 PGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALEL 350

||||||||||||||||||||||||||||||||||||||||||||||||||
P53_CHLAE 301 PGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALEL 350

P53_HUMAN 351 KDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD 393

|||||||||.||||||||||||||||||||||.||||||||||
P53_CHLAE 351 KDAQAGKEPAGSRAHSSHLKSKKGQSTSRHKKFMFKTEGPDSD 393

#---------------------------------------
#---------------------------------------

Transeq (P53 Human Gene)

>c7687490-7668421_1 Homo sapiens chromosome 17, GRCh38.p14 Primary Assembly
LKSLEPPSREQVAAGLRGHFAFGLGACFPRR*HASLDWVSS*LNLMSPL*VTGSRLRVFS
ARENRWGWGWGSGDLASLGVSGMEAWLEGSS*ELHCWETWV*MMGMLGPSELKVERLGRG
VELWGTLSRPKAYFFAHPPGAGRRESLK*KMHKALRSETFGSRNIENS*LYILEPMAS**
KLGLHSEMIIWG*SGEPKLLRSHNFRTFVLPGAIFPGSPRLR*MEKIQLKAVSRGSEKC*
TRGLPARPRRTVAI*EARQPCYCLAPHLHFCLLQQHFRFLFAGAAHYSPDERGGEREKMS
FRPVPLTWQREAAILRLHFFFWIT*LWPLQRQGYLF*CKPQSLPFFEWCAPPRGSPAT*A
DATMA*DREGKKCAEGKPGGIFKNEYISSSRRKKKKNGYV*ESNFERVQ*WVV**FVGKT
IYLLSSFGLGHSSSRRRLNVVKRKGRARRRPCGPPCSPPARAGSSWX