Please watch the video or read the references indicated below and answer the worksheets provided in

this module:
 A tour of microbiology lab: https://www.youtube.com/watch?v=gjvMJ9Gwet4(2:20mins.)
 Laboratory equipment: https://www.youtube.com/watch?v=Dq5JgsxJpTY (3:05mins.)
 How to Focus a Microscope & How the field of view changes:
https://www.youtube.com/watch?v=zzamomqlwxU&t=8s (2:44 mins.)

 Microscopes: resolution, field of view and depth of field: https://www.youtube.com/watch?

v=RnihniihXNQ (4:28 mins.)

 Bartolome FA & Quiles EP. (2020). Microbiology and Parasitology, A Textbook and Laboratory
Manual for the Health Sciences, 2nd Ed. C&E Publishing, Quezon City, Philippines.

 Cowan, Marjorie Kelly (2015). Microbiology: Systems Approach. 4 th edition. McGrawHill

Education: 2 Penn Plaza, New York. pp.55-74.

 Parker, Nina, et.al. (2018).Microbiology.OpenStax Rice University 6100 Main Street MS-375
Houston, Texas 77005.pp.53-70; pp.150-231.)
Answer the following activities:

1. Name the commonly used tool in microbial study and state its function. (20 points)

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1 Photo credit: J.Fenol, USA Biolab

Photo credit: J.Fenol, USA Biolab

Photo credit: J.Fenol, USA Biolab

Photo credit: J.Fenol, USA Biolab

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Photo credit: J.Fenol, USA Biolab

Photo credit: J.Fenol, USA Biolab

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Photo credit: J.Fenol, USA Biolab

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Photo credit: J.Fenol, USA Biolab

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10 Photo credit: J.Fenol, USA Biolab

Photo credit: J.Fenol, USA Biolab

Photo credit: J.Fenol, USA Biolab

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15 Photo credit: J.Fenol, USA Biolab

Photo credit: J.Fenol, USA Biolab Photo credit: J.Fenol, USA Biolab

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Photo credit: J.Fenol, USA Biolab

Photo credit: J.Fenol, USA Biolab

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Photo credit: J.Fenol, USA Biolab

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2. Data retrieval chart on the types of microscopes. (8 points)

 Types of Microscope Distinct features/purpose
a. Brightfield
b. Darkfield
c. Phase-contrast
d. Differential interference contrast
e. Fluorescence
f. Confocal
g. Transmission electron microscope
h. Scanning electron microscope

3. State the function of each part of the compound light microscope. (16 points)
Components Function
1. binocular
2. revolving nosepiece
3. arm
4. coarse adjustment knob
5. fine adjustment knob
6. stage
7. stage clip
8. condenser
9. iris diaphragm
10. illuminator
11. On/off switch
12. base
13. binocular body
14. objective lens
15. Rheostat control knob
16. Diopter adjustment

4. List 6 ways on the proper handling/manipulation of the microscope. (6 points)

A._____________________________________________________________________
B. ____________________________________________________________________
C. ____________________________________________________________________
D. ____________________________________________________________________
E. ____________________________________________________________________
F. ____________________________________________________________________