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1456913254CHE P15 M19 E-Text
1456913254CHE P15 M19 E-Text
3. Oxygen Carriers
Oxygen carriers are special types of metalloproteins which have a transition metal from 3d series
bound to a protein. Only those transition metals can form dioxygen complexes with O2 which
have vacant site/s for binding and exist in higher oxidation states also. The common metal ions
are iron, copper and vanadium and the commonly encountered stoichiometries are 1:1 or 1:2 .
The metal binds reversibly to dioxygen without the metal or ligand being irreversibly oxidized.
More broadly, a complex which can uptake dioxygen and can release it reversibly under ordinary
conditions can be called a biological oxygen carrier. The extent of binding will depend on
temperature, partial pressure of O2 and pH.
nML + O2 ⇌ MLn O2
In the above ML acts as a dioxygen carrier. In order for transition metal complexes to form
dioxygen complexes there must be a vacant site (or sites) for binding and an accessible higher
oxidation state (or states).
There are three known classes of dioxygen transport proteins. These are:
1. Hemocyanins
2. Hemerythrins
3. The Hemoglobin- Myoglobin family
4. Hemocyanin
Hemocyanins (or haemocyanins) are oxygen carrying proteins/oxygen carriers in invertebrates
such as molluscs (eg octopus, snails, squids) and arthropods (eg scorpions, crabs, lobsters etc). It
is extracellular protein and is present in hemolymph.
4.1 Structure
Although the oxygen binding sites and function of molluscan and arthropod hemocyanins are
quite similar, they are different in molecular structure at all levels. Arthropod hemocyanins are
multiples of hexamers, each hexamer made of monomers (Figure 1) Molluscan hemocyanin occur
as larger assemblies.
CHEMISTRY PAPER No. 15: BIOINORGANIC CHEMISTRY
MODULE No. 19: OXYGEN CARRIERS
Figure 1: Single Oxygenated Functional Unit from the hemocyanin of an octopus
Hemocyanin is a copper containing metalloprotein. Each monomer contains two cuprous ions
[Cu(I)] that reversibly bind one dioxygen. An empty cavity is present between the two cuprous
ions to accommodate the dioxygen. The Cu(I)- Cu(I) bond distance is 460 pm. The coordination
number of each Cu(I) is three and is satisfied by three histidines residues from the protein. This
results in a distorted trigonal pyramidal geometry. Two phenylalanine residues which are in close
proximity to the histidines residues provide a hydrophobic environment at the active site.
The binding of dioxygen to the Cu(I) at the active site leads to the following changes
Cu(I) is oxidized to Cu(II).
O2 is reduced to O22-.
Colour of protein changes from colorless to blue.
Coordination number of copper changes to five from three.
Geometry of copper changes to square pyramidal from trigonal pyramidal. The equatorial
plane has two histidyl imidazole nitrogens, the bound oxygens and the third histidyl
nitrogen is axially coordinated to copper.
The Cu-Cu distance decreases to 360pm.
In hemocyanin oxidative addition of dioxygen occurs. Evidence for the peroxo linkage comes
from Raman spectroscopy. The v(O-O) stetch is observed at 744 cm-1 confirming the presence of
peroxo linkage ( Theoretical stretching frequency for peroxo is 740 to 930 cm-1). Experiments
using asymmetrically labeled dioxygen 18O-16O proved conclusively that the coordination
of dioxygen to Cu(II) is symmetrical (Figure 2).
5. Hemerythrin
Hemerythrin (Figure 3) is a reversible oxygen binding metalloprotein found in blood cells of a
few marine invertebrates. It is colorless in the deoxy form and on oxygenation the color changes
to purple-red.
5.1 Structure
It is an oligomeric protein generally found in an octomeric form. The dimeric, trimeric and
tetrameric forms of hemerythrin are also known.
Each monomeric unit contains an active site which has two high spin ferrous ions [Fe(II)]. The
ferrous ions are bridged together by a hydroxyl group and two carboxyl groups from an aspartate
residue and a glutamate residue of the protein chain of 115 amino acid residues. One of the
ferrous is hexacoordinated with an octahedral geometry and the other is pentacoordinated with a
distorted trigonal bipyramidal geometry. The remaining coordination sites of hexacoordinated
ferrous and pentacoordinated ferrous are satisfied by three and two imidazole nitrogens
respectively from histidines residues of the protein chain (Figure 4)
CHEMISTRY PAPER No. 15: BIOINORGANIC CHEMISTRY
MODULE No. 19: OXYGEN CARRIERS
Figure 4: Active site of deoxyhemerythrin
One monomeric unit of hemerythrin binds one dioxygen. The dioxygen adds to hemerythrin in an
oxidative manner resulting in the formation of Fe(III) and peroxide. The dioxygen adds to the
coordinatively unsaturated ferrous. The oxidative addition is followed by the shifting of proton
from the bridged OH to the bound peroxide resulting in the formation of hydroperoxo (HO2-)
group. The hydroperoxo group is hydrogen bonded with the µ-oxo group.
Support for the above structures has been experimentally obtained. By use of radioisotope
experiments, it was established that dioxygen binds asymmetrically in oxyhemerythrin. Single
crystal X-ray diffraction study of oxyhemerythrin showed end –on coordination of dioxygen to
only one iron.
6. Hemoglobin
Hemoglobin is a globular, iron containing metalloprotein with a quaternary structure (64*55*50
Å). It is found in red blood cells (RBC) of mammals and other animals. It transports dioxygen
from the lungs to the tissues, where it is used to oxidize glucose. This process serves as a source
of energy required for cellular metabolic processes. Hemoglobin also plays a role in transport of
carbon dioxide and hydrogen ions. Hemoglobin increases the dioxygen carrying capacity of 1L of
blood from 5mL to 250mL. The dioxygen binding capacity of hemoglobin is 1.34mL O2 per gram
6.1 Structure
The structure of hemoglobin was solved by groups of Andrew Kendrew and Max Perutz. It is a
tetramer with four subunits (Figure 6).
Figure 6: Structure of human hemoglobin. The proteins α and β subunits are in red and blue, and
the iron-containing heme groups in green.
Each subunit of hemoglobin contains a single molecule of heme (Figure 7) and a polypeptide
chain. Heme is Fe(II)- Protoporphyrin complex. Porphyrins are heterocyclic compounds formed
by fusion of four pyrrole rings, linked by methine bridges. Iron is present at the centre of the
protoporphyrin ring.
Different types of hemoglobin are present during fetal and adult life, differing in the kind of
polypeptide chain.
1. Adult hemoglobin (α2β2) represented as HbA or HbA1 is the normal hemoglobin in
adults. It has two α polypeptide chains and two β polypeptide chains. The α chain has 141
residues and β has 146 residues.
2. Minor adult hemoglobin (α2δ2) represented as HbA2 has two α and two δ chains.
3. Fetal hemoglobin (α2γ2) represented as HbF is composed of two α and two γ chains.
A normal adult has all three kinds of hemoglobin in the approximate amounts of about 97.5%
HbA, 2% HbA2 and 0.5% HbF.
The secondary structure of about 75% of amino acids in α or β chains is helical. Each polypeptide
chain contains heme in the heme pocket.
The four subunits are arranged in a tetrahedral manner and the individual polypeptide chains
folded such that maximum number of polar or charged residues are on the outside surface of the
subunit and the non-polar residues are inside. The exposed polar residues make the protein
soluble in aqueous medium and the interior non-polar residues result in the formation of a
hydrophobic pocket in which heme is present. This hydrophobic environment hinders the
oxidation of ferrous ion. The hemoglobin tetramer can be represented as two identical dimers,
each being αβ. Each α chain is in contact with both β chains. There are few interactions between
the two α chains and the two β chains. The two polypeptide chains are held tightly within each
dimer by three kinds of interactions namely hydrophobic, ionic and hydrogen bonding. The
dimers are held together by only ionic bonds and can move with respect to each other.