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Diamonsil AAA Columns

Diamonsil® AAA columns show outstanding selectivity and high resolution. Eighteen kinds of natural amino acids, an internal standard, and
ammonia can be detected simultaneously using PITC and DNFB reagents.

The analysis method is stable and highly reproducible, and can therefore be used for quantitative as well as qualitative analysis.

A variety of interfering factors such as reagents, byproducts, solvents, etc., can be removed by rapid extraction.

Every AAA column passes the test of separation of eighteen natural amino acids.

In proteomics and food quality tests, amino acid analysis (AAA) is often used as a tool to determine the exact composition of the amino acid
(AA) sample, so the improvement of amino acid analysis (AAA) is highly emphasized.

Diamonsil® AAA column is a new product made by Dikma Technologies Inc. which can perform amino acid analysis using two different amino
acid derivative methods upon changing the chromatograph condition.

Eighteen Natural Amino Acids, 2,4-Dinitrophenol,

Eighteen Natural Amino Acids, Norleucine, and NH3– DNFB, Cysteine, 2-Aminobutyric Acid,
PITC Derivatives and NH3-DNFB Derivatives
Column: Diamonsil® 5 μm AAA, 250 x 4.6 mm Column: Diamonsil® 5 μm AAA, 250 x 4.6 mm
Cat. No.: 99751 Cat. No.: 99751
Mobile Phase: A:50 mM Sodium acetate (pH 6.50 +/-0.05, Mobile Phase: A: 20 mM Disodium hydrogen phosphate + 20 mM
adjusted with HClO4) sodium dihydrogen phosphate
B: MeOH:MeCN:H2O= 20:60:20 (v/v/v) B: MeCN:MeOH = 10:90 (v/v)
Flow Rate: 1.0 mL/min Flow Rate: 1.0 mL/min
Temperature: 45 oC Temperature: 35 oC
Injection Volume: 10 μL Injection Volume: 10 μL
Detection: UV 254 nm Detection: UV 360 nm

Gradient: Gradient:

Time/min 0 39 40 45 46 60 Time/min 0 39 44 44.01 65

A 95 52 0 0 95 95 A 86 60 30 86 86
B 5 48 100 100 5 5 B 14 40 70 14 14

1. Asp 11. Tyr 1. Asp 12. Met

2. Glu 12. Val 2. Glu 13. Cys-Cys
3. Ser 13. Met 3. Ser 14. NH3
4. Gly 14. Cys-Cys 4. 2,4-Dinitrophenol 15. Ile
5. His 15. Ile 5. Arg 16. Leu
6. Arg 16. Leu 6. Gly 17. Trp
7. Thr 17. Nle 7. Thr 18. His
8. Ala 18. Phe 8. Pro 19. Phe
9. Pro 19. Trp 9. Ala 20. DNFB
10. NH3 20. Lys 10. 2-Aminobutyric acid 21. Cys
11. Val 22. Lys
23. Tyr
20 4

14 19
11 12 22
1 2 3 4 5 6 78 9 13 15161718
1 23
13 1617
2 3 5 67 18
9 10 1112 15 19
10 21
8 20
14

10 20 30 40 Min.
10 20 30 40 Min.

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Diamonsil AAA Ordering Information
Description Cat.No.
Diamonsil® 5 μm AAA, 250 x 4.6 mm 99751

USA Canada Literature No. 3028

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