ASSIGNMENT -1

QUALITY MANAGEMENT SYSTEM

Submitted by –Sonali

Reg.no-1811302023

B.Sc microbiology honours

6th semester

Submitted to- Aastha Ma’am

Biosafety regulations in microbiology lab

Biosafety- The discipline addressing the safe handling and containment of infectious microorganisms
and hazardous biological materials.

4 CATEGORIES OF BIOSAFETY LEVELS.

1) BSL- 1

2) BSL- 2

3) BSL-3

4) BSL- 4

1) BSL-1-- It is the lowest level; that applies to work with agents that usually pose a minimal
potential threat to laboratory workers and the environment and do not consistently cause disease in
healthy adults.

Research with these agents is generally performed on standard open laboratory benches without the
use of special containment equipment. BSL 1 labs are not usually isolated from the general building.
Training on the specific procedures is given to the lab personnel, who are supervised by a trained
microbiologist or scientist.

Standard microbiology practices are usually enough to protect laboratory workers and other
employees in the building.

Uses

1) Biosafety Level-1 is commonly used while performing tests on microbial agents that are not known
to cause diseases in immune-compromised individuals.

2) These laboratories include the laboratories used for teaching purposes in colleges and training
centers.
2) BSL-2— It covers work with agents associated with human disease, in other words, pathogenic or
infectious organisms posing a moderate hazard. Examples are the equine encephalitis viruses and HIV
when performing routine diagnostic procedures or work with clinical specimens.

Therefore, because of their potential to cause human disease, great care is used to prevent
percutaneous injury, ingestion and mucous membrane exposures in addition to the standard
microbiological practices of BSL 1.

Uses

1) Biosafety level-2 laboratories are mostly used for routine analysis and culture of moderately
hazardous agents.

2) Besides, some of the laboratories used for teaching and training purposes are also BSL-2
laboratories.

3) BSL-3-- Biosafety level 3 (BSL-3) is the level where work is performed with agents that may cause
severe or potentially lethal disease through inhalation or aerosol formation, to the personnel, and may
even contaminate the environment. These laboratories should be certified for use before initial
operation and subsequently on an annual schedule or after a program change

Uses

1) BSL-3 laboratories are used for clinical, diagnostic, teaching, research, or production facilities.

2) These laboratories are used for the handling and manipulation of highly infectious agents that prose
direct severe effects on the health of the personnel.

4) BSL-4-- Agents requiring BSL 4 facilities and practices are extremely dangerous and pose a high
risk of life-threatening disease. Examples are the Ebola virus, the Lassa virus, and any agent with
unknown risks of pathogenicity and transmission. These facilities provide the maximum protection
and containment.

The requirements of BSL-4 laboratories are extensive with specific laboratory design, training
procedures, and highly protective equipment and personal gears.

Uses

1) BSL-4 laboratories are used for diagnostic and research work on easily transmitted pathogens,
causing fatal diseases.

2) These laboratories are used for new and unknown pathogenic microbes, for which no vaccines or
therapies are available.

3) They are also used for clinical and production facilities that require highly sophisticated techniques
and advanced processes.