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Bio Molecules
Bio Molecules
©
© 2022,
2022, Aakash
Aakash BYJU'S.
BYJU'S. All
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rights reserved
reserved
Key Takeaways
1 Biomolecules
Metabolites 2
3 Carbohydrates
Lipids 4
5 Nucleic Acids
7 Metabolism
Enzymes 8
Summary
© 2022, Aakash BYJU'S. All rights reserved
Components of Life
Components of life
Elements
Biomicromolecules Biomacromolecules (Ca, Mg, etc)
Biomolecules
Micromolecules Macromolecules
Metabolites
Types of carbohydrates
Monosaccharides Polysaccharides
Monosaccharides
5 5
HOCH2 OH HOCH2 OH
O O
4 C Deoxygenation 4 C
OH on Carbon 2 H H C 1 H H C 1 No OH on Carbon 2
H C C H H C C H
3 2 3 2
OH OH OH H
H20 +
Homopolysaccharides Heteropolysaccharides
Structural Storage
Peptidoglycan Agar Hyaluronic acid
polysaccharides polysaccharides
Starch
Polymer of glucose
Gives blue colour with iodine Cellulose
Major reserve food in plants Polymer of glucose
Consists of amylopectin and Forms structural part of plants
amylose (cell wall)
Straight chain and unbranched
Glycogen Cotton fibres contain 90% of
Polymer of glucose cellulose
Food reserve in animals
Highly branched
Stored in liver and muscles Chitin
Polymer of N-acetyl-D-
glucosamine
Inulin Form structural part of living
Polymer of fructose organisms (exoskeleton of
Not metabolised in human body arthropods)
Stored in Dahlia, dandelion and Complex polysaccharide
artichoke
Peptidoglycan Agar
Component of bacterial cell wall, which is Obtained from the red algae - Gelidium and
degraded by lysozyme Gracilaria
Made of two different repeating units Agar is a mixture of agarose and agaropectin
o N-Acetyl glucosamine (NAG) Used as medium in labs
o N-Acetyl muramic acid (NAM)
Oligopeptide
Hyaluronic acid
Responsible for the toughness and flexibility of
cartilage and tendon
Made of two different units
o D-glucuronic acid
o N-acetyl-D-glucosamine
NAG
NAM
sugars
Glucose Fructose Galactose
Monosaccharide
Add an equal
amount of
Benedict’s solution
H OH H OH H OH H H HO
OH O O
O OH HO OH H H
HO O
H OH H OH H OH H OH
H OH H
n
OH
Classification of lipids
Neutral/ True
Waxes Phospholipids Glycolipids Lipoprotein Chromolipids
fats
triglyceride
3H2O
Glycerides
C FA C FA C FA
C C FA C FA
C C C FA
Monoglyceride Diglyceride Triglyceride
OH
Non-essential fatty
O acids: Can be
Arachidonic
acid (20 Carbon synthesised by the
atoms) body
Palmitic acid (16 Carbon atoms)
Simple lipids
Fats Oils
Esters of fatty acids Esters of long chain Mainly from Mainly from plant
and glycerol fatty acids and fatty animal sources
E.g.,- Butter, ghee, alcohol sources
oils E.g.,- Bee wax
Differences
Relatively Relatively more
more unsaturated
saturated
Compound lipids
Steroids do not contain fatty acids yet have fat like CH3 CH3
properties
Most common steroids are sterols CH3
Complex in structure
HO
E.g., Cholesterol
o Most common sterols Cholesterol
o Tetracyclic in nature
o Essential component of animal plasma membrane, also
synthesised in live
E.g., Prostaglandins
o Derived from arachidonic acid
o Group of hormone which function as messenger
substance between the cell
Functions of lipids
Chemical messengers
(hormones)
Major component of membranes
(phospholipids)
Monomers of
Nucleic acids Nucleotide nucleic acid
Deoxyribonucleic
Ribonucleic acid
acid
Nucleoside Phosphate
Nitrogenous
Pentose sugar base
Nucleotide
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Nucleic Acids
Pentose sugar
5-Carbon monosaccharide
Central molecule in a nucleotide
Ribose Deoxyribose
Heterocyclic Nitrogen-
Nitrogenous bases containing compounds
Purines Pyrimidines
H H H H H
H
Adenine (A) Guanine (G) Cytosine (C) Uracil (U) Thymine (T)
In both DNA and RNA In DNA, cytosine and thymine are found
In RNA, cytosine and uracil are found
Phosphodiester Bond
-
Ester bond formed between
the phosphate group of one
nucleotide and hydroxyl
group of the sugar of the next
nucleotide
Phosphodiester Connecting link between two
bond
- consecutive nucleotides
Backbone of DNA
Antiparallel strands
0.34 nm 3’
5’
Minor
groove
Major
groove
3.4 nm
5’
3’
Phosphodiester H- Bonds
bond
Chargaff’s rule
● Concluded by Erwin Chargaff for ● (A+T)/ (G+C) constant for a given
DNA molecule species only
● (A + G) Purines = Pyrimidine (T + C) ● Equal proportion of deoxyribose
● A = T and G = C sugar and pentose sugar
RNA
Usually single stranded but sometimes double stranded
(Reovirus and Rice dwarf virus)
Does not follow Chargaff’s rule
Forms of RNA
H CH3 CH2 OH H O
H
Glycine Alanine Serine
N C C
H O
R=H R = CH3 R = CH2-OH
R
Based on number of amine and carboxyl groups Based on the presence of aromatic ring
COOH H H O O O
H H H
H2N C COOH H2N C C OH H2N C C OH H2N C C OH
CH2 H2N C COOH
CH2
CH2 CH2 CH2
CH2 H
CH2
COOH CH2 HN
NH2
OH
Aspartic acid Lysine Phenylalanine Tryptophan Tyrosine
Amino Acids
Phenylalanine Proline
Valine Alanine
Essential Non-essential
Threonine Glycine
Obtained through
Synthesised by Tryptophan Glutamate
diet; body does
the body
not synthesise
Isoleucine Cysteine
Methionine Serine
Semi-essential amino acid:
Lysine
Synthesised very slowly by human
beings.
Zwitterions:
Molecule with one functional group having positive charge and the other
having negative charge
Positive charge = Negative charge
Net charge = Zero
-NH2 is a strong base and can Amino acids Zwitterions
pick up protons (H+) from
Cation Zwitterion Anion
-COOH group
○ Due to this, NH2 acquires +
NH3
+
NH3 NH2
positive charge (NH3+ ) and - -
● There are 20 types amino acids, a protein is a heteropolymer and not a homopolymer.
N C H N C H N C N C H
H C O H C O H C C O
H H H H
R R R R
C-terminal N-terminal
Structure of proteins
Primary structure
Secondary structure
Complexity
Tertiary structure
Quaternary structure
Primary structure
Linear chain of amino acids
Positional information
N- Terminal : Free Amine group with alpha carbon
C-Terminal : Free Carboxyl group with alpha carbon
H H H
R H
R R R
N-terminal amino acid
Secondary structure
Polypeptide
Segments of
chain folds in the
polypeptide chain
form of right-
line up next to each
handed helices
other resembling
resembling a
pleated paper.
spring. E.g.,
α-helices E.g.,Fibroin β-sheets
Keratin
Polypeptide 1
Polypeptide 2
Fibrous Globular
Note:
Most abundant animal protein: Collagen
Most abundant protein in the biosphere: RuBisCO
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Metabolism
Metabolism
Anabolism Catabolism
Energy
Energy
ATP
Triphosphate
Adenine Ribose
Absorb Release
energy energy
ADP
Diphosphate
Adenine Ribose
CH2 Adenine
CH2 Adenine
Energy ADP
released Ribose
Acetyl - CoA
CoA Citric Acid (6C)
Metabolic pathways CAA
NAD+
can be cyclic (4C)
NADH
(E.g., Krebs cycle) CO2
NADH
NAD+
NAD+
NADH
FADH2 CO2
FAD
ADP +1P
ATP
Glucose
ATP
ADP
ATP
Unstable ADP
P P
Metabolic pathways Fructose-1, 6-bisphosphate
can be linear
P P
(E.g., Glycolysis) DHAP Glyceraldehyde - 3 -phosphate
NAD+D
All the DHAP will be
NADH
converted into
glyceraldehyde-3- ADP
phosphate ATP
ADP
ATP
Pyruvate
Zn tightly bound
carboxypeptidase
Without catalyst:
H2CO3-
CO2 H2O
200 molecules
With catalyst:
carbonic
anhydrase
CO2 H2O H2CO3-
2,160,000,000 molecules
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Chemical Reaction
Chemical Reaction
Endothermic Exothermic
E E
n n
e e Heat of reaction
r Products r Reactants
g g
y Reactants y Products
Mechanism of action
Activation
energy:
Substrate (S) + Enzyme (E)
The difference in
free energy
between the
E-S Complex transition state
and that of
reactant
E-P Complex
Enzyme (E) + Product (P) Enzymes increase the rate of reaction by:
Decreasing the activation energy
Forming weak bonds with the substrate
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Enzymes
Enzyme-Substrate complex:
Two important model have been proposed to describe the enzyme and substrate binding.
Temperature pH
High temperature : Denaturation ● Enzymes are sensitive to pH
Low temperature : Temporary ● Rise or fall in pH : reduces enzyme activity
inactive state ● Optimum pH : maximum action
100%
30
Rate of reaction
active enzyme
Temperature
Percentage
1
increases activity 1 2
Optimum rate
20 Denaturation
2
decreases activity
0
0%
10 20 30 40 50 60
Temperature / C
o
Substrate concentration
Low substrate concentration:
Low enzyme activity
VMax
Optimum substrate
concentration: Maximum
enzyme activity
Higher substrate concentration: ½ VMax
No change in maximum activity
KM (Michaelis constant) :
reaction
Rate of
Indicates the substrate
concentration at which the
chemical reaction catalysed by Substrate
an enzyme attains half its KM concentration
maximum velocity (Vmax)
Inhibitors
Competitive inhibitors
Non-competitive inhibitors
Normal
Substrate
Non competitive
Inhibitor
Allosteric modulation
Normal
Substrate
Metabolites
Types of carbohydrates
Monosaccharides Polysaccharides
Classification of lipids
Neutral/
Waxes
True fats Phospholipids Glycolipids Lipoprotein Chromolipids
Summary
Amino acids
Amino acids
Valine Alanine
Obtained through
Synthesised by
diet; body does
the body Threonine Glycine
not synthesise
Tryptophan Glutamate
Nucleic acids
Monomers of
Nucleic acids Nucleotide nucleic acid
Deoxyribonucleic
Ribonucleic acid
acid
Nucleoside Phosphate
Nitrogenous
Pentose sugar base
Nucleotide
© 2022, Aakash BYJU'S. All rights reserved
Summary
Backbone of DNA
Antiparallel strands
Forms of DNA
(with right handed coiling)
Forms of RNA
Types of proteins
Fibrous Globular
Based on function
Inhibitors