Updating Method of Water Microbiology Parameters

20 Sep 2023

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Speaker Introduction
Wan Teng is a seasoned professional
in the water industry. With a career
spanning back to 2013, Wan Teng
embarked on her journey in this vital
sector with a water treatment
company. There, she excelled in a
multitude of supporting functions,
seamlessly blending technical
expertise with commercial acumen.

Beginning as a Technical Support

Specialist, she lend her expertise to
support the dynamic needs of
countries in Southeast Asia. Her
passion and proficiency soon
Wong Wan Teng propelled her to her current role,
Technical Sales, SEA Water where she spearheads technical
sales in the bustling hub of
Singapore.

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Waterborne diseases caused by bacteria
Disease Causal Bacterial Agent

Escherichia coli, particularly serotypes:

O124 (EIEC: entero-invasive E. coli)
Acute diarrheas and gastroenteritis O148 (ETEC: entero-toxigenic E. coli)
O157 (EHEC: entero-haemoragic E. coli)

Gastrointestinal illnesses associated Enterococcus

with swimming in affected waters
Respiratory, skin, blood, heart,
gastrointestinal and urinary tract
infections
Pseudomonas aeruginosa
Swimmer’s ear, hot tub rash

Legionnaires' disease Legionella pneumophila

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Water-borne diseases in the news

• >200 cases reported in 2 provinces in • As of 11 September 2023, a total of 166 cases

Philippines (Negros Occidental and of legionellosis, including 23 deaths
Kalinga Provinces) between Jan-Feb
• Causal Bacterial Agent: Legionella pneumophila
2023
• Water samples were collected from the water
• Coliform and E-coli bacteria have infected supply system, residences of the sick,
people through contaminated food and healthcare facilities, cooling towers, and
water installations in buildings to determine the source
© 2023 IDEXX Laboratories, Inc. All rights reserved. of infection
IDEXX Solutions
Coliform and E. coli Enterococci P. aeruginosa L. pneumophila Quality Control
Colilert® Enterolert® Pseudalert™ Legiolert® Quanti-Cult®
Colilert®-18 Enterolert®-E Pseudalert™ 250 Water QC
Colisure® Enterolert®-DW
Colilert ® 250 Enterolert® 250
Tecta Tecta
Cryptosporidium/ Heterotrophic
Quantification SARS-CoV-2 Accessories
Giardia Plate Count
Filta-Max® SimPlate® for HPC Quanti-Tray® DNA/RNA Sample
Filta-Max xpress® HPC for Quanti- Quanti-Tray®/2000 Extraction collection
Invitrogen Dynabeads® tray® Quanti- RT-qPCR vessels
for IMS EasyDisc PCA Tray/Legiolert Incubators
EasyDisc YEA Sealer+
EasyDisc R2A Tecta

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Worldwide Water Product Approvals/Acceptances

© 2023 IDEXX Laboratories, Inc. All rights reserved. © 2018 IDEXX Laboratories, Inc. All rights reserved. 6
Indonesia Drinking Water and Hygiene and
Sanitation Requirements

o Permenkes no 2 Tahun 2023

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Colilert-18

o Simultaneously detects
coliforms & E.coli in 18
hours

o No confirmation tests
required

o Can be used for both

presence/absence and
quantitative

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Standard Method 9223B (24th Edition)
Colilert, Colilert-18, Colisure

o Standard Method 9223

• Enzyme Substrate Coliform Test

o Section 9223B
• Enzyme Substrate Test

© 2023 IDEXX Laboratories, Inc. All rights reserved.

ISO 9308-2:2012
Colilert-18

o ISO 9308-2:2012
• Water quality - enumeration of
Escherichia coli and coliform bacteria
• Part 2: Most probable number method

o Part 2: most probable number method

• IDEXX Colilert-18/Quanti-Tray

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Indonesia Bathing Water Requirements

o Permenkes no 2 Tahun 2023

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Enterolert

o Detects enterococci in 24
hours

o No confirmation steps
required

o Can be used for both

presence/absence and
quantitative

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Standard Method 9230D (24th Edition)
Enterolert

o Standard Method 9230

• Fecal Enterococcus/
Streptococcus Groups

o Section 9230D
• Fluorogenic Substrate
Enterococcus Test

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Indonesia Swimming Pool and Spa Water
Requirements
o Permenkes no 2 Tahun 2023

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Pseudalert
Product Details

o Detect Pseudomonas
aeruginosa in 24 hours

o No confirmation steps required

o Can be used for both

presence/absence and
quantitative

o Presence/Absence for 100 mL

or 250 mL

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Standard Method 9213G (24th Edition)
Pseudalert, Pseudalert-250

o Standard Method 9213

• 24th Edition (2023)

o Section 9223G
• Enzyme Substrate Test for
Pseudomonas Aeruginosa

© 2023 IDEXX Laboratories, Inc. All rights reserved.

ISO 16266-2
Pseudalert, Pseudalert-250

o ISO 16266-2:2018
• Water Quality – Detection and
enumeration of Pseudomonas
aeruginosa

o Part 2:
• Most probable number method

© 2023 IDEXX Laboratories, Inc. All rights reserved.

MPN & CFU are interchangeable per ISO 6107:2021
definition
Acceptable units to estimate the
number of cultural microorganisms:

- CFU
- MPN

CFU and MPN are interchangeable!

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Sample Report – LGC (UK lab)

Source: LGC (UK lab) – Aug 2023

IDEXX product used:

1. Colilert-18
2. Enterolert

CFU and MPN are

interchangeable!

© 2023 IDEXX Laboratories, Inc. All rights reserved.

Additional Information
o Product display and demonstration can be seen at the back of the
ballroom
o Technical team support available locally by PT Thermalindo
o Complimentary Verification training for existing IDEXX customers
who want to get ISO 17025 accreditation for their Water
Microbiology scope
o QC kits are available for purchase now

© 2023 IDEXX Laboratories, Inc. All rights reserved.

© 2023 IDEXX Laboratories, Inc. All rights reserved.