RID: NWCBBJYD016

Job Title: DinoDNA "Dinosaur DNA" from Crichton JURASSIC

Program: BLASTN
Database: nt Nucleotide collection (nt)
Query #1: DinoDNA "Dinosaur DNA" from Crichton JURASSIC PARK p. 103 nt 1-1200
Query ID: lcl|Query_82843 Length: 1200

Sequences producing significant alignments:

Scientific
Common Max Total Query E Per. Acc.
Description Name
Name Taxid Score Score cover Value Ident Len Accession
Vector pACTEM1, complete sequence Vector pACTEM1
NA 2724077 537 1346 97% 4e-147 87.80 5841 MN386081.1
Expression vector pCJH5_pDEST17-EcG3PD, complete sequence Expression v...
NA 2682822 537 1760 99% 4e-147 87.80 5734 MK910753.1
Expression vector pDEST17-AceKMs, complete sequence Expression v...
NA 2682826 537 1760 99% 4e-147 87.80 5842 MK910749.1
Expression vector pDM1, complete sequence Expression v...
NA 2654422 537 1346 97% 4e-147 87.80 5584 MN128719.1
Cloning vector pENTR-BsaI-Tet LI, complete sequence Cloning vect...
NA 2597298 537 1552 86% 4e-147 87.80 4308 MK495753.1
Escherichia coli strain Mach1 plasmid pSS1129, complete sequence Escherichia ...
NA 562 537 1760 99% 4e-147 87.80 9690 MN057686.1
Cloning vector pAgaL6, complete sequence Cloning vect...
NA 2487903 537 1760 99% 4e-147 87.80 7989 MH621333.1
Cloning vector pAgaL5, complete sequence Cloning vect...
NA 2487902 537 1760 99% 4e-147 87.80 8136 MH621332.1
Cloning vector pAgaL4, complete sequence Cloning vect...
NA 2487901 537 1760 99% 4e-147 87.80 7998 MH621331.1
Cloning vector pAgaL3, complete sequence Cloning vect...
NA 2487900 537 1760 99% 4e-147 87.80 8187 MH621330.1
Cloning vector pAgaL2, complete sequence Cloning vect...
NA 2487899 537 1760 99% 4e-147 87.80 9834 MH621329.1
Cloning vector pAgaL1, complete sequence Cloning vect...
NA 2487898 537 1760 99% 4e-147 87.80 7551 MH621328.1
Cloning vector pTLD39, complete sequence Cloning vect...
NA 2479792 537 1700 96% 4e-147 87.80 76343 MG711910.1
Cloning vector pTLD67, complete sequence Cloning vect...
NA 2479793 537 1700 96% 4e-147 87.80 72952 MG711909.1
Cloning vector pSS4162, complete sequence Cloning vect...
NA 2293876 537 1760 99% 4e-147 87.80 12539 MH521154.1
Cloning vector pSS3967, complete sequence Cloning vect...
NA 2293875 537 1760 99% 4e-147 87.80 15046 MH521153.1
Cloning vector pQC2319, complete sequence Cloning vect...
NA 2293732 537 1760 99% 4e-147 87.80 7015 MH587015.1
Cloning vector pQC2123, complete sequence Cloning vect...
NA 2293731 537 1760 99% 4e-147 87.80 11256 MH587014.1
Synthetic construct polyprotein gene, complete cds synthetic co...
NA 32630 537 1760 99% 4e-147 87.80 15193 KY583506.1
Transformation vector pRCATGNT Transformati...
NA 2077197 537 716 39% 4e-147 87.80 32361 KY766997.1
Expression vector pRLP121, complete sequence Expression v...
NA 2070483 537 1760 99% 4e-147 87.80 7078 MF688774.1
Expression vector pTMH308, complete sequence Expression v...
NA 2070485 537 1760 99% 4e-147 87.80 7150 MF688773.1
Expression vector pTMH307, complete sequence Expression v...
NA 2070484 537 1760 99% 4e-147 87.80 7150 MF688772.1
Cloning vector pET15b, complete sequence Cloning vect...
NA 1982515 537 1760 99% 4e-147 87.80 5803 KX765180.1
Cloning vector pAxCALRL, complete sequence Cloning vect...
NA 1977086 537 4423 99% 4e-147 87.80 45883 KX529075.1
Cloning vector pAxCALGL, complete sequence Cloning vect...
NA 1940841 537 4423 99% 4e-147 87.80 45889 KX589554.1
Vector pATcE6Hy3, complete sequence Vector pATcE...
NA 1945242 537 1760 99% 4e-147 87.80 4713 LT727631.1
Vector pIVHA, complete sequence Vector pIVHA
NA 1945322 537 1760 99% 4e-147 87.80 6249 LT727608.1
Vector pSVT, complete sequence Vector pSVT
NA 1945410 537 1760 99% 4e-147 87.80 6805 LT727602.1
Vector pBRcHIL6f3, complete sequence Vector pBRcH...
NA 1945261 537 1760 99% 4e-147 87.80 5324 LT727600.1
Vector pBRcHIL6f2, complete sequence Vector pBRcH...
NA 1945260 537 1760 99% 4e-147 87.80 5491 LT727599.1
Vector pBRcHIL6f1, complete sequence Vector pBRcH...
NA 1945259 537 1760 99% 4e-147 87.80 5360 LT727598.1
Vector pATIPMC, complete sequence Vector pATIPMC
NA 1945248 537 1760 99% 4e-147 87.80 4904 LT727590.1
Vector pATIPMA, complete sequence Vector pATIPMA
NA 1945247 537 1760 99% 4e-147 87.80 4904 LT727589.1
Vector pBRE6L, complete sequence Vector pBRE6L
NA 1945262 537 1760 99% 4e-147 87.80 5290 LT727583.1
Vector pIPM, complete sequence Vector pIPM
NA 1945320 537 1760 99% 4e-147 87.80 5497 LT727582.1
Vector pATHIFNBg41, complete sequence Vector pATHI...
NA 1945243 537 1760 99% 4e-147 87.80 5210 LT727581.1
Vector pATMIFNG, complete sequence Vector pATMIFNG
NA 1945249 537 1760 99% 4e-147 87.80 4822 LT727580.1
Bacterial expression vector pET-3c, complete sequence Bacterial ex...
NA 1944668 537 1760 99% 4e-147 87.80 4638 LT727578.1
Bacterial expression vector pET-3a, complete sequence Bacterial ex...
NA 1944666 537 1760 99% 4e-147 87.80 4640 LT727576.1
Bacterial expression vector pET-3b, complete sequence Bacterial ex...
NA 1944667 537 1760 99% 4e-147 87.80 4639 LT727575.1
Vector pBRHIFNGf0, complete sequence Vector pBRHI...
NA 1945264 537 1745 98% 4e-147 87.80 4199 LT727573.1
Vector pAT153HIL2, complete sequence Vector pAT15...
NA 1945240 537 1760 99% 4e-147 87.80 4385 LT727571.1
Vector pBRphoA, complete sequence Vector pBRphoA
NA 1945265 537 1760 99% 4e-147 87.80 4562 LT727562.1
Vector pSC2D, complete sequence Vector pSC2D
NA 1945391 537 1735 98% 4e-147 87.80 9962 LT727554.1
Yeast expression vector pEX2, complete sequence Yeast expres...
NA 1945470 537 1735 98% 4e-147 87.80 9214 LT727553.1
Yeast expression vector pEX0, complete sequence Yeast expres...
NA 1945469 537 1735 98% 4e-147 87.80 7511 LT727552.1
Vector pATHIL6HIFNG, complete sequence Vector pATHI...
NA 1945244 537 1760 99% 4e-147 87.80 6207 LT727534.1
Vector pATHIL6pA, complete sequence Vector pATHI...
NA 1945245 537 1760 99% 4e-147 87.80 5382 LT727532.1
Vector pATHTNF, complete sequence Vector pATHTNF
NA 1945246 537 1760 99% 4e-147 87.80 5310 LT727524.1
Vector pATMTNF1, complete sequence Vector pATMTNF1
NA 1945251 537 1760 99% 4e-147 87.80 5208 LT727523.1
Vector pIDUHA, complete sequence Vector pIDUHA
NA 1945319 537 1760 99% 4e-147 87.80 6065 LT727513.1
Vector pATSVTMx, complete sequence Vector pATSVTMx
NA 1945253 537 1760 99% 4e-147 87.80 6441 LT727509.1
Vector pATMx, complete sequence Vector pATMx
NA 1945252 537 1760 99% 4e-147 87.80 4112 LT727508.1
Fungal expression vector pSal43A, complete sequence Fungal expre...
NA 1944734 537 1745 98% 4e-147 87.80 5873 LT727506.1
Bacterial expression vector pET-11c, complete sequence Bacterial ex...
NA 1944665 537 1760 99% 4e-147 87.80 5675 LT727491.1
Bacterial expression vector pET-11b, complete sequence Bacterial ex...
NA 1944664 537 1760 99% 4e-147 87.80 5676 LT727490.1
Bacterial expression vector pET-11a, complete sequence Bacterial ex...
NA 1944663 537 1760 99% 4e-147 87.80 5677 LT727489.1
Bacterial expression vector pJBtetT7POL, complete sequence Bacterial ex...
NA 1944680 537 1145 49% 4e-147 87.80 13318 LT727466.1
Vector pT7POL23, complete sequence Vector pT7POL23
NA 1945411 537 716 39% 4e-147 87.80 11164 LT727456.1
Cloning vector pKT240tet2, complete sequence Cloning vect...
NA 1944718 537 888 49% 4e-147 87.80 9277 LT727452.1
Bacterial expression vector pJBtrc, complete sequence Bacterial ex...
NA 1944681 537 888 49% 4e-147 87.80 7952 LT727449.1
Cloning vector pKT240tet1, complete sequence Cloning vect...
NA 1944717 537 888 49% 4e-147 87.80 12950 LT727446.1
Bacterial expression vector pJB3tet2, complete sequence Bacterial ex...
NA 1944676 537 888 49% 4e-147 87.80 6440 LT727434.1
Bacterial expression vector pJB3tet1, complete sequence Bacterial ex...
NA 1944675 537 888 49% 4e-147 87.80 6840 LT727433.1
Vector pLPPsOmpArPDI, complete sequence Vector pLPPs...
NA 1945326 537 1760 99% 4e-147 87.80 8486 LT727428.1
Vector pTRKH1, complete sequence Vector pTRKH1
NA 1945414 537 1321 96% 4e-147 87.80 10948 LT727421.1
Vector pBRgHIL61, complete sequence Vector pBRgH...
NA 1945263 537 2388 99% 4e-147 87.80 9484 LT727419.1
Bacterial expression vector pPLc236, complete sequence Bacterial ex...
NA 1944692 537 1760 99% 4e-147 87.80 4425 LT727393.1
Vector pATmIL6hIFNG, complete sequence Vector pATmI...
NA 1945250 537 1760 99% 4e-147 87.80 5856 LT727371.1
Bacterial expression vector pJBpn25lacZ, complete sequence Bacterial ex...
NA 1944679 537 888 49% 4e-147 87.80 12509 LT727366.1
Bacterial expression vector pJBtrc10, complete sequence Bacterial ex...
NA 1944682 537 888 49% 4e-147 87.80 8463 LT727349.1
Bacterial expression vector pJBtrc2, complete sequence Bacterial ex...
NA 1944683 537 888 49% 4e-147 87.80 7718 LT727348.1
Mammalian expression vector pACCMVpLpA, complete sequence Mammalian ex...
NA 1944745 537 1760 99% 4e-147 87.80 8796 LT727333.1
Bacterial expression vector pJE202, complete sequence Bacterial ex...
NA 1944684 537 1760 99% 4e-147 87.80 13009 LT727306.1
Vector pTRKH3, complete sequence Vector pTRKH3
NA 1945415 537 1316 95% 4e-147 87.80 7766 LT727213.1
Vector pMC1403, complete sequence Vector pMC1403
NA 1945344 537 1760 99% 4e-147 87.80 9886 LT727182.1
Vector pAT153MIL2F2, complete sequence Vector pAT15...
NA 1945241 537 1760 99% 4e-147 87.80 4664 LT727177.1
Vector pSEVA512S-16S-pad, complete sequence Vector pSEVA...
NA 3056412 537 666 36% 4e-147 87.80 5513 ON366567.1
Synthetic construct His-TEV-Nsp15 gene, complete cds synthetic co...
NA 32630 537 1760 99% 4e-147 87.80 6760 ON191567.1
Cloning vector pET-RA-Y, complete sequence Cloning vect...
NA 2910771 537 1733 98% 4e-147 87.80 6817 OK655938.1
Expression vector p15Tc-litR, complete sequence Expression v...
NA 2885347 537 1346 97% 4e-147 87.80 5337 MZ683493.1
Synthetic construct Golden Gate selectable marker insert TetR... synthetic co...
NA 32630 537 801 44% 4e-147 87.80 1881 OK165508.1
Cloning vector pRK177, complete sequence Cloning vect...
NA 2759567 537 1530 87% 4e-147 87.80 11938 MN626476.1
Vector pKW20_CDFtet_pAraRedCas9_TracrRNA, complete sequence Vector pKW20...
NA 2823284 537 1088 76% 4e-147 87.80 10272 MN927219.1
Cloning vector pET14b_17AECD2C_StarStuff-L-PhaC, complete... Cloning vect...
NA 2826910 537 1760 99% 4e-147 87.80 7869 MW151040.1
Cloning vector pET14b_17AECD3C_Pipsqueaks-L-PhaC, complete... Cloning vect...
NA 2826911 537 1760 99% 4e-147 87.80 7896 MW151039.1
Cloning vector pET14b_17AECD5C_JAWS-L-PhaC, complete sequence Cloning vect...
NA 2826913 537 1760 99% 4e-147 87.80 7260 MW151038.1
Cloning vector pET14b_17AECD6C_Inca-L-PhaC, complete sequence Cloning vect...
NA 2826914 537 1760 99% 4e-147 87.80 7245 MW151037.1
Cloning vector pET14b_17AECEBC_Giles-L-PhaC, complete sequence Cloning vect...
NA 2826917 537 1760 99% 4e-147 87.80 7623 MW151036.1
Cloning vector pET14b_17AECD7C_Dylan-L-PhaC, complete sequence Cloning vect...
NA 2826915 537 1760 99% 4e-147 87.80 7428 MW151035.1
Cloning vector pET14b_17AECD4C_D29-L-PhaC, complete sequence Cloning vect...
NA 2826912 537 1760 99% 4e-147 87.80 7152 MW151034.1
Cloning vector pET14b_17AECEAC_Bongo-L-PhaC, complete sequence Cloning vect...
NA 2826916 537 1760 99% 4e-147 87.80 7359 MW151033.1
Cloning vector pC15-plsg, complete sequence Cloning vect...
NA 2594799 537 1346 97% 4e-147 87.80 15280 MK353498.2
Vector pET19b_ShHTL7cpGFP(L166)LSSmOrange, complete sequence Vector pET19...
NA 2818171 537 1760 99% 4e-147 87.80 7892 MT270503.1
Vector pET19b_HisDAD2cpGFP(G166), complete sequence Vector pET19...
NA 2818170 537 1760 99% 4e-147 87.80 7175 MT270502.1
Vector pET19b_HisDAD2, complete sequence Vector pET19...
NA 2818169 537 1760 99% 4e-147 87.80 6431 MT270501.1
Vector pET19b_DAD2cpGFP(G166)LSSmOrange, complete sequence Vector pET19...
NA 2818168 537 1760 99% 4e-147 87.80 7880 MT270500.1
Vector pET19b_DAD2cpGFP(G166), complete sequence Vector pET19...
NA 2818167 537 1760 99% 4e-147 87.80 7136 MT270499.1
Cloning vector mini-RGC, complete sequence Cloning vect...
NA 2813847 537 1673 94% 4e-147 87.80 7974 MW148404.1

Alignments:

>Vector pACTEM1, complete sequence

Sequence ID: MN386081.1 Length: 5841
Range 1: 3180 to 3589

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3589 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3530

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3529 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3470

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3469 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3410

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

 ||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3409 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3350

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3349 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3290

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3289 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3230

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3229 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3180

Range 2: 2903 to 3163

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3163 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3104

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3103 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3044

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3043 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 2984

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 2983 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2924

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2923 TCTCGGGCAGCGTTGGGTCCT 2903

Range 3: 4690 to 4998

Score:233 bits(258), Expect:6e-56,

Identities:242/317(76%), Gaps:39/317(12%), Strand: Plus/Minus

Query 680 CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----------CAAGTCAGA 729

||||||||||||||||||||||||||||| |||||||| || ||| ||||
Sbjct 4998 CGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT 4939

Query 730 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGG----------A 779

|||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4938 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCG 4879
Query 780 AGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG 839
||||||||||||||| || | | | ||||||| | | ||||| || | | ||
Sbjct 4878 TGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGG-TGTCATTCCGCTGTTATGGC--CGC 4822

Query 840 GCTT-TCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC 898

| || ||||| | ||||| | |||||||| | ||||| |||||||||||||
Sbjct 4821 GTTTGTCTCATT--CCACGC---CTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGC 4767

Query 899 TG----------ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC 948

|| ||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 4766 TGGACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATC 4707

Query 949 GTCTTGAGTCCAACACG 965

|||||||||||||| ||
Sbjct 4706 GTCTTGAGTCCAACCCG 4690

Range 4: 4686 to 4998

Score:224 bits(248), Expect:3e-53,

Identities:242/319(76%), Gaps:35/319(10%), Strand: Plus/Minus

Query 11 CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC---------- 60
||||||||||||||||||||||||||||| |||||||| ||| |||||
Sbjct 4998 CGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT 4939

Query 61 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG 120

||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct 4938 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCG 4879

Query 121 TG-----TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGC 175

|| | | | ||||| ||| | | |||| || | || | | || || ||
Sbjct 4878 TGCGCTCTCCTGTTCCTGC--CTTTCGGTTTACCGGT-GTCATTCCGCTGTTATGGCCGC 4822

Query 176 G--TGGCTGCT--CACGCTGTACCTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTG 231

| || || | ||||| || |||||||| | ||||| |||||||||||||||
Sbjct 4821 GTTTGTCTCATTCCACGCCTGAC---ACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTG 4765

Query 232 GGCTGTGTG----------CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGT 281

| |||| || |||||||| ||||||||||||||||||||||||||||||||
Sbjct 4764 GACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGT 4705

Query 282 CTTGAGTCCAACCCGGTAA 300

|||||||||||||||| ||
Sbjct 4704 CTTGAGTCCAACCCGGAAA 4686

>Expression vector pCJH5_pDEST17-EcG3PD, complete sequence

Sequence ID: MK910753.1 Length: 5734
Range 1: 4938 to 5347

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5347 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 5288

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5287 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 5228

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 5227 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 5168

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 5167 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5108

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5107 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5048

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5047 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 4988

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4987 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 4938

Range 2: 3244 to 3583

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3583 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3524

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3523 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3464

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3463 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3404

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3403 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3344

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3343 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3284

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3283 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3244
Range 3: 3248 to 3587

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3587 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3528

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3527 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3468

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3467 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3408

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3407 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3348

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3347 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3288

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3287 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3248

Range 4: 4661 to 4921

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4921 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 4862

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4861 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 4802

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4801 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 4742

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 4741 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 4682

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 4681 TCTCGGGCAGCGTTGGGTCCT 4661
>Expression vector pDEST17-AceKMs, complete sequence
Sequence ID: MK910749.1 Length: 5842
Range 1: 5046 to 5455

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5455 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 5396

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5395 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 5336

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 5335 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 5276

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 5275 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5216

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5215 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5156

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5155 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5096

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5095 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5046

Range 2: 3352 to 3691

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3691 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3632

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3631 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3572

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3571 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3512
Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210
|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3511 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3452

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3451 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3392

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3391 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3352

Range 3: 3356 to 3695

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3695 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3636

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3635 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3576

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3575 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3516

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3515 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3456

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3455 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3396

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3395 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3356

Range 4: 4769 to 5029

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5029 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 4970

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4969 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 4910
Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129
| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4909 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 4850

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 4849 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 4790

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 4789 TCTCGGGCAGCGTTGGGTCCT 4769

>Expression vector pDM1, complete sequence

Sequence ID: MN128719.1 Length: 5584
Range 1: 2253 to 2662

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2253 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 2312

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2313 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 2372

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 2373 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 2432

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 2433 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 2492

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 2493 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 2552

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2553 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 2612

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2613 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 2662

Range 2: 2679 to 2939

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus
Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023
||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2679 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 2738

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2739 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 2798

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2799 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 2858

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 2859 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2918

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2919 TCTCGGGCAGCGTTGGGTCCT 2939

Range 3: 844 to 1152

Score:233 bits(258), Expect:6e-56,

Identities:242/317(76%), Gaps:39/317(12%), Strand: Plus/Plus

Query 680 CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----------CAAGTCAGA 729

||||||||||||||||||||||||||||| |||||||| || ||| ||||
Sbjct 844 CGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT 903

Query 730 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGG----------A 779

|||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 904 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCG 963

Query 780 AGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG 839

||||||||||||||| || | | | ||||||| | | ||||| || | | ||
Sbjct 964 TGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGG-TGTCATTCCGCTGTTATGGC--CGC 1020

Query 840 GCTT-TCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC 898

| || ||||| | ||||| | |||||||| | ||||| |||||||||||||
Sbjct 1021 GTTTGTCTCATT--CCACGC---CTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGC 1075

Query 899 TG----------ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC 948

|| ||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 1076 TGGACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATC 1135

Query 949 GTCTTGAGTCCAACACG 965

|||||||||||||| ||
Sbjct 1136 GTCTTGAGTCCAACCCG 1152

Range 4: 844 to 1156

Score:224 bits(248), Expect:3e-53,

Identities:242/319(76%), Gaps:35/319(10%), Strand: Plus/Plus
Query 11 CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC---------- 60
||||||||||||||||||||||||||||| |||||||| ||| |||||
Sbjct 844 CGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT 903

Query 61 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG 120

||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct 904 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCG 963

Query 121 TG-----TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGC 175

|| | | | ||||| ||| | | |||| || | || | | || || ||
Sbjct 964 TGCGCTCTCCTGTTCCTGC--CTTTCGGTTTACCGGT-GTCATTCCGCTGTTATGGCCGC 1020

Query 176 G--TGGCTGCT--CACGCTGTACCTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTG 231

| || || | ||||| || |||||||| | ||||| |||||||||||||||
Sbjct 1021 GTTTGTCTCATTCCACGCCTGAC---ACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTG 1077

Query 232 GGCTGTGTG----------CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGT 281

| |||| || |||||||| ||||||||||||||||||||||||||||||||
Sbjct 1078 GACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGT 1137

Query 282 CTTGAGTCCAACCCGGTAA 300

|||||||||||||||| ||
Sbjct 1138 CTTGAGTCCAACCCGGAAA 1156

>Cloning vector pENTR-BsaI-Tet LI, complete sequence

Sequence ID: MK495753.1 Length: 4308
Range 1: 753 to 1162

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 753 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 812

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 813 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 872

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 873 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 932

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 933 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 992

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 993 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1052

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1053 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1112
Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1113 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1162

Range 2: 1568 to 1907

Score:443 bits(490), Expect:7e-119,

Identities:298/340(88%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1907 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1848

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1847 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1788

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1787 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1728

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| ||||||||||||| |||||||||||||||
Sbjct 1727 CTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTG 1668

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1667 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 1608

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 1607 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 1568

Range 3: 1572 to 1911

Score:443 bits(490), Expect:7e-119,

Identities:298/340(88%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1911 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1852

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1851 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1792

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1791 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1732

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 1731 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 1672
Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925
||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1671 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 1612

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 1611 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 1572

Range 4: 1179 to 1271

Score:128 bits(141), Expect:7e-24,

Identities:83/93(89%), Gaps:10/93(10%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCC---------- 1019

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1179 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1238

Query 1020 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGA 1052

|||||||||||||||||||||||||||||||||
Sbjct 1239 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGA 1271

>Escherichia coli strain Mach1 plasmid pSS1129, complete sequence

Sequence ID: MN057686.1 Length: 9690
Range 1: 3575 to 3984

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3984 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3925

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3924 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3865

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3864 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3805

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3804 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3745

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3744 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3685

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3684 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3625
Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3624 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3575

Range 2: 1881 to 2220

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2220 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2161

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2160 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2101

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2100 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2041

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2040 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 1981

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1980 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 1921

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 1920 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 1881

Range 3: 1885 to 2224

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2224 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2165

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2164 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2105

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2104 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2045

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2044 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 1985
Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925
||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1984 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 1925

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 1924 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 1885

Range 4: 3298 to 3558

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3558 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3499

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3498 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3439

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3438 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3379

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3378 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 3319

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 3318 TCTCGGGCAGCGTTGGGTCCT 3298

>Cloning vector pAgaL6, complete sequence

Sequence ID: MH621333.1 Length: 7989
Range 1: 5770 to 6179

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6179 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 6120

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6119 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 6060

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 6059 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 6000
Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511
||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 5999 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5940

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5939 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5880

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5879 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5820

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5819 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5770

Range 2: 4076 to 4415

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4415 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 4356

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4355 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 4296

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 4295 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4236

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4235 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4176

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4175 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4116

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4115 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4076

Range 3: 4080 to 4419

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4419 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 4360
Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775
|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4359 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 4300

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4299 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4240

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4239 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4180

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4179 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4120

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4119 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4080

Range 4: 5493 to 5753

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5753 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5694

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5693 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5634

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5633 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5574

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5573 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5514

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 5513 TCTCGGGCAGCGTTGGGTCCT 5493

>Cloning vector pAgaL5, complete sequence

Sequence ID: MH621332.1 Length: 8136
Range 1: 5917 to 6326

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus
Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6326 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 6267

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6266 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 6207

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 6206 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 6147

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 6146 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 6087

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 6086 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 6027

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 6026 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5967

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5966 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5917

Range 2: 4223 to 4562

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4562 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 4503

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4502 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 4443

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 4442 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4383

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4382 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4323

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4322 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4263

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4262 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4223
Range 3: 4227 to 4566

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4566 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 4507

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4506 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 4447

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4446 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4387

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4386 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4327

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4326 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4267

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4266 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4227

Range 4: 5640 to 5900

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5900 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5841

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5840 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5781

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5780 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5721

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5720 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5661

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 5660 TCTCGGGCAGCGTTGGGTCCT 5640

>Cloning vector pAgaL4, complete sequence

Sequence ID: MH621331.1 Length: 7998
Range 1: 5779 to 6188

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6188 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 6129

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6128 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 6069

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 6068 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 6009

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 6008 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5949

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5948 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5889

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5888 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5829

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5828 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5779

Range 2: 4085 to 4424

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4424 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 4365

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4364 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 4305

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 4304 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4245
Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210
|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4244 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4185

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4184 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4125

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4124 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4085

Range 3: 4089 to 4428

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4428 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 4369

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4368 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 4309

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4308 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4249

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4248 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4189

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4188 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4129

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4128 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4089

Range 4: 5502 to 5762

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5762 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5703

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5702 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5643

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5642 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5583

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5582 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5523

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 5522 TCTCGGGCAGCGTTGGGTCCT 5502

>Cloning vector pAgaL3, complete sequence

Sequence ID: MH621330.1 Length: 8187
Range 1: 5968 to 6377

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6377 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 6318

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6317 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 6258

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 6257 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 6198

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 6197 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 6138

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 6137 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 6078

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 6077 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 6018

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6017 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5968

Range 2: 4274 to 4613

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus
Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4613 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 4554

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4553 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 4494

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 4493 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4434

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4433 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4374

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4373 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4314

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4313 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4274

Range 3: 4278 to 4617

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4617 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 4558

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4557 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 4498

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4497 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4438

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4437 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4378

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4377 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4318

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4317 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4278
Range 4: 5691 to 5951

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5951 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5892

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5891 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5832

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5831 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5772

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5771 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5712

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 5711 TCTCGGGCAGCGTTGGGTCCT 5691

>Cloning vector pAgaL2, complete sequence

Sequence ID: MH621329.1 Length: 9834
Range 1: 7615 to 8024

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8024 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 7965

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7964 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 7905

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 7904 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 7845

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 7844 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 7785

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 7784 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 7725

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 7724 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 7665
Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7664 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 7615

Range 2: 5921 to 6260

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6260 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 6201

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6200 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 6141

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 6140 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 6081

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 6080 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 6021

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 6020 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 5961

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 5960 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 5921

Range 3: 5925 to 6264

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6264 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 6205

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6204 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 6145

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6144 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 6085

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 6084 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 6025

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 6024 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 5965

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 5964 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 5925

Range 4: 7338 to 7598

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7598 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 7539

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7538 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 7479

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7478 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 7419

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 7418 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 7359

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 7358 TCTCGGGCAGCGTTGGGTCCT 7338

>Cloning vector pAgaL1, complete sequence

Sequence ID: MH621328.1 Length: 7551
Range 1: 5332 to 5741

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5741 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 5682

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5681 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 5622

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 5621 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 5562
Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511
||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 5561 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5502

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5501 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5442

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5441 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5382

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5381 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5332

Range 2: 3638 to 3977

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3977 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3918

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3917 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3858

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3857 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3798

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3797 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3738

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3737 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3678

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3677 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3638

Range 3: 3642 to 3981

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3981 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3922

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3921 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3862

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3861 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3802

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3801 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3742

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3741 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3682

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3681 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3642

Range 4: 5055 to 5315

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5315 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5256

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5255 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5196

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5195 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5136

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5135 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5076

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 5075 TCTCGGGCAGCGTTGGGTCCT 5055

>Cloning vector pTLD39, complete sequence

Sequence ID: MG711910.1 Length: 76343
Range 1: 74566 to 74975

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus
Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 74566 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 74625

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 74626 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 74685

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 74686 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 74745

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 74746 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 74805

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 74806 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 74865

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 74866 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 74925

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 74926 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 74975

Range 2: 1 to 324

Score:409 bits(453), Expect:1e-108,

Identities:281/324(87%), Gaps:40/324(12%), Strand: Plus/Plus

Query 17 TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC----------GGTGGC 66
|||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct 1 TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC 60

Query 67 GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG------ 120

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 61 GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT 120

Query 121 ----TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG 176

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 121 CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG 180

Query 177 TGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA 226

|||| |||||||||||| |||||||||||||||||||||||||||||||
Sbjct 181 TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA 240

Query 227 AGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT 276

|||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 241 AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT 300

Query 277 ATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||
Sbjct 301 ATCGTCTTGAGTCCAACCCGGTAA 324

Range 3: 1 to 320

Score:402 bits(445), Expect:2e-106,

Identities:277/320(87%), Gaps:40/320(12%), Strand: Plus/Plus

Query 686 TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----------CAAGTCAGAGGTGGC 735

||||||||||||||||||||||||||||||||||| |||||||||||||||
Sbjct 1 TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC 60

Query 736 GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA----------GCGCT 785

||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct 61 GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT 120

Query 786 CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG------ 839

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 121 CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG 180

Query 840 ----GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA 895

||||||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 181 TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA 240

Query 896 AGCTG----------ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT 945

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 241 AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT 300

Query 946 ATCGTCTTGAGTCCAACACG 965

||||||||||||||||| ||
Sbjct 301 ATCGTCTTGAGTCCAACCCG 320

Range 4: 74992 to 75252

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 74992 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 75051

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 75052 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 75111

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 75112 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 75171

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 75172 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 75231

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

 |||||||||||||||||||||
Sbjct 75232 TCTCGGGCAGCGTTGGGTCCT 75252

>Cloning vector pTLD67, complete sequence

Sequence ID: MG711909.1 Length: 72952
Range 1: 71175 to 71584

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 71175 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 71234

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 71235 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 71294

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 71295 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 71354

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 71355 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 71414

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 71415 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 71474

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 71475 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 71534

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 71535 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 71584

Range 2: 1 to 324

Score:409 bits(453), Expect:1e-108,

Identities:281/324(87%), Gaps:40/324(12%), Strand: Plus/Plus

Query 17 TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC----------GGTGGC 66
|||||||||||||||||||||||||||||||||||||||||||| ||||||
Sbjct 1 TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC 60

Query 67 GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG------ 120

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 61 GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT 120

Query 121 ----TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG 176

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 121 CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG 180

Query 177 TGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA 226

|||| |||||||||||| |||||||||||||||||||||||||||||||
Sbjct 181 TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA 240

Query 227 AGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT 276

|||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 241 AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT 300

Query 277 ATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||
Sbjct 301 ATCGTCTTGAGTCCAACCCGGTAA 324

Range 3: 1 to 320

Score:402 bits(445), Expect:2e-106,

Identities:277/320(87%), Gaps:40/320(12%), Strand: Plus/Plus

Query 686 TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----------CAAGTCAGAGGTGGC 735

||||||||||||||||||||||||||||||||||| |||||||||||||||
Sbjct 1 TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC 60

Query 736 GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA----------GCGCT 785

||||||||||||||||||||||||||||||||||||||||||||| |||||
Sbjct 61 GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT 120

Query 786 CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG------ 839

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 121 CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG 180

Query 840 ----GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA 895

||||||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 181 TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA 240

Query 896 AGCTG----------ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT 945

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 241 AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT 300

Query 946 ATCGTCTTGAGTCCAACACG 965

||||||||||||||||| ||
Sbjct 301 ATCGTCTTGAGTCCAACCCG 320

Range 4: 71601 to 71861

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 71601 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 71660

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 71661 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 71720

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 71721 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 71780

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 71781 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 71840

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 71841 TCTCGGGCAGCGTTGGGTCCT 71861

>Cloning vector pSS4162, complete sequence

Sequence ID: MH521154.1 Length: 12539
Range 1: 8954 to 9363

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8954 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 9013

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9014 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 9073

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 9074 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 9133

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 9134 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 9193

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 9194 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 9253

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 9254 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 9313

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9314 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 9363

Range 2: 10716 to 11055

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 10716 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 10775

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 10776 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 10835

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 10836 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 10895

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 10896 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 10955

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 10956 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 11015

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 11016 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 11055

Range 3: 10712 to 11051

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 10712 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 10771

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 10772 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 10831

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 10832 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 10891

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 10892 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 10951

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 10952 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 11011

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 11012 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 11051
Range 4: 9380 to 9640

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9380 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 9439

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9440 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 9499

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9500 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 9559

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 9560 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 9619

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 9620 TCTCGGGCAGCGTTGGGTCCT 9640

>Cloning vector pSS3967, complete sequence

Sequence ID: MH521153.1 Length: 15046
Range 1: 11461 to 11870

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11461 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 11520

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11521 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 11580

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 11581 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 11640

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 11641 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 11700

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 11701 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 11760

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 11761 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 11820

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11821 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 11870

Range 2: 13223 to 13562

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 13223 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 13282

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 13283 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 13342

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 13343 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 13402

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 13403 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 13462

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 13463 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 13522

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 13523 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 13562

Range 3: 13219 to 13558

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 13219 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 13278

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 13279 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 13338

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 13339 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 13398

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

 |||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 13399 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 13458

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 13459 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 13518

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 13519 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 13558

Range 4: 11887 to 12147

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11887 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 11946

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11947 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 12006

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 12007 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 12066

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 12067 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 12126

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 12127 TCTCGGGCAGCGTTGGGTCCT 12147

>Cloning vector pQC2319, complete sequence

Sequence ID: MH587015.1 Length: 7015
Range 1: 3430 to 3839

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3430 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3489

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3490 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3549

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3550 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3609

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3610 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3669

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3670 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3729

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3730 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3789

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3790 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3839

Range 2: 5192 to 5531

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5192 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 5251

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5252 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 5311

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 5312 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 5371

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 5372 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 5431

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 5432 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 5491

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 5492 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 5531

Range 3: 5188 to 5527

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

 || ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5188 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 5247

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5248 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 5307

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5308 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 5367

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 5368 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 5427

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 5428 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 5487

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 5488 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 5527

Range 4: 3856 to 4116

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3856 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3915

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3916 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3975

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3976 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 4035

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 4036 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 4095

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 4096 TCTCGGGCAGCGTTGGGTCCT 4116

>Cloning vector pQC2123, complete sequence

Sequence ID: MH587014.1 Length: 11256
Range 1: 7671 to 8080

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7671 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 7730

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7731 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 7790

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 7791 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 7850

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 7851 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 7910

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 7911 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 7970

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 7971 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 8030

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8031 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 8080

Range 2: 9433 to 9772

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9433 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 9492

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9493 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 9552

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 9553 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 9612

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 9613 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 9672

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 9673 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 9732

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

 ||||||||||||||||||||||||||||||||||||||||
Sbjct 9733 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 9772

Range 3: 9429 to 9768

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9429 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 9488

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9489 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 9548

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9549 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 9608

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 9609 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 9668

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 9669 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 9728

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 9729 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 9768

Range 4: 8097 to 8357

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8097 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 8156

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8157 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 8216

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8217 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 8276

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 8277 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 8336
Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200
|||||||||||||||||||||
Sbjct 8337 TCTCGGGCAGCGTTGGGTCCT 8357

>Synthetic construct polyprotein gene, complete cds

Sequence ID: KY583506.1 Length: 15193
Range 1: 11593 to 12002

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11593 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 11652

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11653 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 11712

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 11713 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 11772

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 11773 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 11832

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 11833 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 11892

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 11893 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 11952

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11953 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 12002

Range 2: 13355 to 13694

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 13355 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 13414

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 13415 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 13474

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

 |||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 13475 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 13534

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 13535 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 13594

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 13595 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 13654

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 13655 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 13694

Range 3: 13351 to 13690

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 13351 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 13410

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 13411 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 13470

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 13471 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 13530

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 13531 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 13590

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 13591 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 13650

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 13651 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 13690

Range 4: 12019 to 12279

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 12019 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 12078
Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079
||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 12079 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 12138

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 12139 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 12198

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 12199 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 12258

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 12259 TCTCGGGCAGCGTTGGGTCCT 12279

>Transformation vector pRCATGNT

Sequence ID: KY766997.1 Length: 32361
Range 1: 21431 to 21840

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 21431 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 21490

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 21491 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 21550

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 21551 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 21610

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 21611 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 21670

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 21671 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 21730

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 21731 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 21790

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 21791 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 21840

Range 2: 21857 to 21977

Score:178 bits(197), Expect:4e-39,
Identities:111/121(92%), Gaps:10/121(8%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 21857 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 21916

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 21917 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 21976

Query 1080 G 1080

|
Sbjct 21977 G 21977

>Expression vector pRLP121, complete sequence

Sequence ID: MF688774.1 Length: 7078
Range 1: 1351 to 1760

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1760 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1701

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1700 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1641

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1640 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1581

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1580 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1521

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1520 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1461

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1460 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1401

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1400 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1351

Range 2: 6735 to 7074

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7074 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 7015

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7014 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 6955

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 6954 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 6895

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 6894 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 6835

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 6834 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 6775

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 6774 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 6735

Range 3: 6739 to 7078

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7078 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 7019

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7018 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 6959

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6958 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 6899

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 6898 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 6839

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 6838 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 6779

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 6778 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 6739
Range 4: 1074 to 1334

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1334 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1275

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1274 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1215

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1214 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1155

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1154 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1095

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1094 TCTCGGGCAGCGTTGGGTCCT 1074

>Expression vector pTMH308, complete sequence

Sequence ID: MF688773.1 Length: 7150
Range 1: 1351 to 1760

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1760 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1701

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1700 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1641

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1640 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1581

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1580 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1521

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1520 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1461

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1460 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1401

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1400 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1351

Range 2: 6807 to 7146

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7146 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 7087

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7086 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 7027

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 7026 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 6967

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 6966 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 6907

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 6906 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 6847

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 6846 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 6807

Range 3: 6811 to 7150

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7150 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 7091

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7090 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 7031

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7030 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 6971

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

 |||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 6970 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 6911

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 6910 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 6851

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 6850 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 6811

Range 4: 1074 to 1334

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1334 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1275

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1274 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1215

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1214 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1155

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1154 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1095

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1094 TCTCGGGCAGCGTTGGGTCCT 1074

>Expression vector pTMH307, complete sequence

Sequence ID: MF688772.1 Length: 7150
Range 1: 1351 to 1760

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1760 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1701

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1700 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1641

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1640 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1581

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1580 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1521

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1520 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1461

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1460 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1401

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1400 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1351

Range 2: 6807 to 7146

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7146 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 7087

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7086 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 7027

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 7026 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 6967

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 6966 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 6907

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 6906 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 6847

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 6846 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 6807

Range 3: 6811 to 7150

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

 || ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7150 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 7091

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7090 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 7031

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7030 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 6971

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 6970 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 6911

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 6910 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 6851

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 6850 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 6811

Range 4: 1074 to 1334

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1334 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1275

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1274 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1215

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1214 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1155

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1154 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1095

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1094 TCTCGGGCAGCGTTGGGTCCT 1074

>Cloning vector pET15b, complete sequence

Sequence ID: KX765180.1 Length: 5803
Range 1: 3773 to 4182

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4182 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 4123

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4122 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 4063

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 4062 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 4003

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 4002 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3943

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3942 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3883

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3882 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3823

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3822 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3773

Range 2: 2079 to 2418

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2418 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2359

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2358 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2299

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2298 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2239

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2238 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2179

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2178 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2119

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

 ||||||||||||||||||||||||||||||||||||||||
Sbjct 2118 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2079

Range 3: 2083 to 2422

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2422 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2363

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2362 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2303

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2302 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2243

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2242 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2183

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2182 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2123

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2122 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2083

Range 4: 3496 to 3756

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3756 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3697

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3696 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3637

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3636 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3577

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3576 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 3517
Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200
|||||||||||||||||||||
Sbjct 3516 TCTCGGGCAGCGTTGGGTCCT 3496

>Cloning vector pAxCALRL, complete sequence

Sequence ID: KX529075.1 Length: 45883
Range 1: 5799 to 6208

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6208 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 6149

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6148 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 6089

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 6088 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 6029

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 6028 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5969

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5968 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5909

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5908 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5849

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5848 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5799

Range 2: 7846 to 8255

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8255 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 8196

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8195 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 8136

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

 ||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 8135 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 8076

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 8075 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 8016

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 8015 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 7956

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 7955 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 7896

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7895 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 7846

Range 3: 9893 to 10302

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 10302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 10243

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 10242 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 10183

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 10182 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 10123

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 10122 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 10063

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 10062 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 10003

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 10002 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 9943

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9942 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 9893

Range 4: 11940 to 12349

Score:537 bits(595), Expect:4e-147,
Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 12349 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 12290

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 12289 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 12230

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 12229 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 12170

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 12169 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 12110

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 12109 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 12050

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 12049 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 11990

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11989 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 11940

Range 5: 5194 to 5533

Score:443 bits(490), Expect:7e-119,

Identities:298/340(88%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5533 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 5474

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5473 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 5414

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 5413 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 5354

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| ||||||||||||| |||||||||||||||
Sbjct 5353 CTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTG 5294

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 5293 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 5234
Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300
||||||||||||||||||||||||||||||||||||||||
Sbjct 5233 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 5194

Range 6: 5198 to 5537

Score:443 bits(490), Expect:7e-119,

Identities:298/340(88%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5537 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 5478

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5477 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 5418

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5417 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 5358

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 5357 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 5298

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 5297 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 5238

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 5237 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 5198

Range 7: 7569 to 7829

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7829 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 7770

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7769 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 7710

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7709 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 7650

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 7649 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 7590

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 7589 TCTCGGGCAGCGTTGGGTCCT 7569

Range 8: 9616 to 9876

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9876 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 9817

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9816 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 9757

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9756 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 9697

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 9696 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 9637

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 9636 TCTCGGGCAGCGTTGGGTCCT 9616

Range 9: 11663 to 11923

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11923 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 11864

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11863 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 11804

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11803 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 11744

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 11743 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 11684

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

 |||||||||||||||||||||
Sbjct 11683 TCTCGGGCAGCGTTGGGTCCT 11663

Range 10: 5528 to 5782

Score:334 bits(370), Expect:2e-86,

Identities:224/255(88%), Gaps:30/255(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5782 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5723

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5722 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5663

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5662 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5603

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5602 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5543

Query 1180 TCTCGGGCAGCGTTG 1194

|||||||| ||||||
Sbjct 5542 TCTCGGGCCGCGTTG 5528

>Cloning vector pAxCALGL, complete sequence

Sequence ID: KX589554.1 Length: 45889
Range 1: 5805 to 6214

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6214 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 6155

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6154 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 6095

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 6094 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 6035

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 6034 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5975

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5974 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5915

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5914 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5855

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5854 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5805

Range 2: 7852 to 8261

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8261 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 8202

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8201 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 8142

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 8141 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 8082

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 8081 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 8022

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 8021 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 7962

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 7961 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 7902

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7901 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 7852

Range 3: 9899 to 10308

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 10308 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 10249

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

 |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 10248 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 10189

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 10188 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 10129

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 10128 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 10069

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 10068 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 10009

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 10008 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 9949

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9948 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 9899

Range 4: 11946 to 12355

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 12355 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 12296

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 12295 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 12236

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 12235 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 12176

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 12175 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 12116

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 12115 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 12056

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 12055 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 11996

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11995 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 11946
Range 5: 5200 to 5539

Score:443 bits(490), Expect:7e-119,

Identities:298/340(88%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5539 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 5480

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5479 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 5420

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 5419 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 5360

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| ||||||||||||| |||||||||||||||
Sbjct 5359 CTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTG 5300

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 5299 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 5240

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 5239 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 5200

Range 6: 5204 to 5543

Score:443 bits(490), Expect:7e-119,

Identities:298/340(88%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5543 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 5484

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5483 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 5424

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5423 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 5364

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||||||||||||||||||||||||||||||
Sbjct 5363 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 5304

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 5303 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 5244
Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965
||||||||||||||||||||||||||||||||||||| ||
Sbjct 5243 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 5204

Range 7: 7575 to 7835

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7835 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 7776

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7775 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 7716

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7715 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 7656

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 7655 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 7596

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 7595 TCTCGGGCAGCGTTGGGTCCT 7575

Range 8: 9622 to 9882

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9882 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 9823

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9822 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 9763

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9762 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 9703

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 9702 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 9643

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 9642 TCTCGGGCAGCGTTGGGTCCT 9622

Range 9: 11669 to 11929

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11929 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 11870

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11869 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 11810

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11809 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 11750

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 11749 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 11690

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 11689 TCTCGGGCAGCGTTGGGTCCT 11669

Range 10: 5534 to 5788

Score:334 bits(370), Expect:2e-86,

Identities:224/255(88%), Gaps:30/255(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5788 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5729

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5728 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5669

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5668 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5609

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5608 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5549

Query 1180 TCTCGGGCAGCGTTG 1194

|||||||| ||||||
Sbjct 5548 TCTCGGGCCGCGTTG 5534
>Vector pATcE6Hy3, complete sequence
Sequence ID: LT727631.1 Length: 4713
Range 1: 1813 to 2222

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1813 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1872

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1873 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1932

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1933 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1992

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1993 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 2052

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 2053 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 2112

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2113 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 2172

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2173 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 2222

Range 2: 2872 to 3211

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2872 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2931

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2932 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2991

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2992 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3051

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3052 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3111

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3112 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3171

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3172 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3211

Range 3: 2868 to 3207

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2868 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2927

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2928 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2987

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2988 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3047

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3048 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3107

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3108 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3167

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3168 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3207

Range 4: 2239 to 2499

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2239 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 2298

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2299 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 2358

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

 | |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2359 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 2418

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 2419 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2478

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2479 TCTCGGGCAGCGTTGGGTCCT 2499

>Vector pIVHA, complete sequence

Sequence ID: LT727608.1 Length: 6249
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 2520 to 2859

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2520 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2579

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2580 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2639

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2640 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2699

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2700 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2759

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2760 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2819

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2820 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2859

Range 3: 2516 to 2855

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2516 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2575

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2576 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2635

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2636 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2695

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2696 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2755

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2756 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2815

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2816 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2855

Range 4: 1184 to 1444

Score:350 bits(387), Expect:1e-90,
Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444

>Vector pSVT, complete sequence

Sequence ID: LT727602.1 Length: 6805
Range 1: 3202 to 3611

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3202 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3261

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3262 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3321

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3322 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3381

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3382 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3441

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3442 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3501

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3502 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3561

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3562 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3611

Range 2: 4964 to 5303

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4964 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 5023

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5024 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 5083

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 5084 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 5143

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 5144 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 5203

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 5204 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 5263

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 5264 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 5303

Range 3: 4960 to 5299

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4960 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 5019

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5020 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 5079

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5080 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 5139

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 5140 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 5199

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

 ||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 5200 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 5259

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 5260 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 5299

Range 4: 3628 to 3888

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3628 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3687

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3688 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3747

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3748 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3807

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3808 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 3867

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 3868 TCTCGGGCAGCGTTGGGTCCT 3888

>Vector pBRcHIL6f3, complete sequence

Sequence ID: LT727600.1 Length: 5324
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 2520 to 2859

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2520 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2579

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2580 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2639

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2640 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2699

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2700 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2759

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2760 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2819

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2820 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2859

Range 3: 2516 to 2855

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2516 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2575

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

 |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2576 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2635

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2636 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2695

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2696 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2755

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2756 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2815

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2816 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2855

Range 4: 1184 to 1444

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444

>Vector pBRcHIL6f2, complete sequence

Sequence ID: LT727599.1 Length: 5491
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 2520 to 2859

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2520 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2579

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2580 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2639

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2640 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2699

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2700 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2759

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2760 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2819

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2820 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2859
Range 3: 2516 to 2855

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2516 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2575

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2576 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2635

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2636 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2695

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2696 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2755

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2756 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2815

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2816 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2855

Range 4: 1184 to 1444

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444
>Vector pBRcHIL6f1, complete sequence
Sequence ID: LT727598.1 Length: 5360
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 2520 to 2859

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2520 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2579

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2580 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2639

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2640 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2699

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

 |||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2700 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2759

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2760 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2819

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2820 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2859

Range 3: 2516 to 2855

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2516 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2575

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2576 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2635

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2636 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2695

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2696 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2755

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2756 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2815

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2816 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2855

Range 4: 1184 to 1444

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303
Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129
| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444

>Vector pATIPMC, complete sequence

Sequence ID: LT727590.1 Length: 4904
Range 1: 2004 to 2413

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2004 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 2063

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2064 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 2123

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 2124 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 2183

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 2184 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 2243

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 2244 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 2303

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2304 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 2363

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2364 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 2413

Range 2: 3063 to 3402

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3063 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3122

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3123 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3182

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3183 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3242

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3243 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3302

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3303 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3362

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3363 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3402

Range 3: 3059 to 3398

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3059 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3118

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3119 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3178

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3179 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3238

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3239 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3298

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3299 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3358

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3359 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3398

Range 4: 2430 to 2690

Score:350 bits(387), Expect:1e-90,
Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2430 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 2489

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2490 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 2549

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2550 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 2609

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 2610 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2669

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2670 TCTCGGGCAGCGTTGGGTCCT 2690

>Vector pATIPMA, complete sequence

Sequence ID: LT727589.1 Length: 4904
Range 1: 2004 to 2413

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2004 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 2063

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2064 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 2123

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 2124 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 2183

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 2184 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 2243

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 2244 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 2303

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2304 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 2363

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

 ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2364 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 2413

Range 2: 3063 to 3402

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3063 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3122

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3123 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3182

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3183 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3242

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3243 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3302

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3303 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3362

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3363 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3402

Range 3: 3059 to 3398

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3059 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3118

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3119 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3178

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3179 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3238

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3239 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3298
Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925
||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3299 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3358

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3359 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3398

Range 4: 2430 to 2690

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2430 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 2489

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2490 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 2549

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2550 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 2609

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 2610 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2669

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2670 TCTCGGGCAGCGTTGGGTCCT 2690

>Vector pBRE6L, complete sequence

Sequence ID: LT727583.1 Length: 5290
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

 ||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 2520 to 2859

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2520 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2579

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2580 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2639

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2640 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2699

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2700 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2759

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2760 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2819

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2820 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2859

Range 3: 2516 to 2855

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2516 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2575
Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775
|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2576 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2635

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2636 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2695

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2696 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2755

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2756 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2815

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2816 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2855

Range 4: 1184 to 1444

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444

>Vector pIPM, complete sequence

Sequence ID: LT727582.1 Length: 5497
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 2520 to 2859

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2520 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2579

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2580 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2639

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2640 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2699

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2700 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2759

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2760 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2819

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2820 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2859
Range 3: 2516 to 2855

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2516 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2575

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2576 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2635

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2636 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2695

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2696 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2755

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2756 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2815

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2816 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2855

Range 4: 1184 to 1444

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444
>Vector pATHIFNBg41, complete sequence
Sequence ID: LT727581.1 Length: 5210
Range 1: 2310 to 2719

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2310 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 2369

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2370 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 2429

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 2430 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 2489

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 2490 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 2549

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 2550 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 2609

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2610 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 2669

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2670 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 2719

Range 2: 3369 to 3708

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3369 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3428

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3429 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3488

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3489 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3548
Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210
|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3549 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3608

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3609 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3668

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3669 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3708

Range 3: 3365 to 3704

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3365 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3424

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3425 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3484

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3485 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3544

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3545 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3604

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3605 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3664

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3665 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3704

Range 4: 2736 to 2996

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2736 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 2795

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2796 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 2855
Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129
| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2856 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 2915

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 2916 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2975

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2976 TCTCGGGCAGCGTTGGGTCCT 2996

>Vector pATMIFNG, complete sequence

Sequence ID: LT727580.1 Length: 4822
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 1817 to 2156

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus
Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1817 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1876

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1877 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1936

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1937 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1996

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 1997 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2056

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2057 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2116

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2117 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2156

Range 3: 1813 to 2152

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1813 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1872

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1873 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1932

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1933 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1992

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 1993 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2052

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2053 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2112

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2113 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2152
Range 4: 1184 to 1444

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444

>Bacterial expression vector pET-3c, complete sequence

Sequence ID: LT727578.1 Length: 4638
Range 1: 1033 to 1442

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1033 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1092

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1093 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1152

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1153 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1212

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1213 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1272

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1273 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1332

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1333 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1392
Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1393 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1442

Range 2: 2797 to 3136

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2797 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2856

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2857 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2916

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2917 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2976

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2977 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3036

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3037 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3096

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3097 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3136

Range 3: 2793 to 3132

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2793 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2852

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2853 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2912

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2913 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2972

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2973 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3032
Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925
||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3033 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3092

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3093 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3132

Range 4: 1459 to 1719

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1459 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1518

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1519 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1578

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1579 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1638

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1639 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1698

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1699 TCTCGGGCAGCGTTGGGTCCT 1719

>Bacterial expression vector pET-3a, complete sequence

Sequence ID: LT727576.1 Length: 4640
Range 1: 1035 to 1444

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1035 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1094

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1095 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1154

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1155 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1214
Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511
||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1215 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1274

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1275 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1334

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1335 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1394

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1395 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1444

Range 2: 2799 to 3138

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2799 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2858

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2859 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2918

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2919 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2978

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2979 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3038

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3039 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3098

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3099 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3138

Range 3: 2795 to 3134

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2795 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2854
Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775
|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2855 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2914

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2915 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2974

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2975 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3034

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3035 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3094

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3095 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3134

Range 4: 1461 to 1721

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1461 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1520

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1521 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1580

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1581 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1640

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1641 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1700

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1701 TCTCGGGCAGCGTTGGGTCCT 1721

>Bacterial expression vector pET-3b, complete sequence

Sequence ID: LT727575.1 Length: 4639
Range 1: 1034 to 1443

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus
Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1034 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1093

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1094 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1153

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1154 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1213

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1214 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1273

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1274 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1333

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1334 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1393

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1394 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1443

Range 2: 2798 to 3137

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2798 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2857

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2858 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2917

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2918 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2977

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2978 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3037

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3038 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3097

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3098 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3137
Range 3: 2794 to 3133

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2794 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2853

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2854 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2913

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2914 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2973

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2974 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3033

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3034 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3093

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3094 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3133

Range 4: 1460 to 1720

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1460 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1519

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1520 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1579

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1580 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1639

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1640 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1699

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1700 TCTCGGGCAGCGTTGGGTCCT 1720

>Vector pBRHIFNGf0, complete sequence

Sequence ID: LT727573.1 Length: 4199
Range 1: 1683 to 2092

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1683 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1742

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1743 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1802

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1803 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1862

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1863 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1922

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1923 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1982

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1983 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 2042

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2043 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 2092

Range 2: 2358 to 2697

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2358 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2417

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2418 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2477

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2478 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2537
Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210
|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2538 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2597

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2598 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2657

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2658 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2697

Range 3: 2354 to 2693

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2354 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2413

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2414 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2473

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2474 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2533

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2534 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2593

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2594 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2653

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2654 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2693

Range 4: 2109 to 2363

Score:334 bits(370), Expect:2e-86,

Identities:224/255(88%), Gaps:30/255(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2109 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 2168

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2169 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 2228

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2229 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 2288

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 2289 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2348

Query 1180 TCTCGGGCAGCGTTG 1194

|||||||| ||||||
Sbjct 2349 TCTCGGGCCGCGTTG 2363

>Vector pAT153HIL2, complete sequence

Sequence ID: LT727571.1 Length: 4385
Range 1: 1485 to 1894

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1485 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1544

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1545 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1604

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1605 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1664

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1665 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1724

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1725 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1784

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1785 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1844

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1845 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1894

Range 2: 2544 to 2883

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus
Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2544 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2603

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2604 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2663

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2664 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2723

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2724 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2783

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2784 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2843

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2844 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2883

Range 3: 2540 to 2879

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2540 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2599

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2600 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2659

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2660 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2719

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2720 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2779

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2780 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2839

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2840 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2879
Range 4: 1911 to 2171

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1911 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1970

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1971 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 2030

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2031 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 2090

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 2091 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2150

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2151 TCTCGGGCAGCGTTGGGTCCT 2171

>Vector pBRphoA, complete sequence

Sequence ID: LT727562.1 Length: 4562
Range 1: 959 to 1368

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 959 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1018

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1019 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1078

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1079 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1138

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1139 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1198

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1199 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1258

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1259 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1318
Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1319 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1368

Range 2: 2721 to 3060

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2721 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2780

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2781 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2840

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2841 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2900

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2901 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2960

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2961 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3020

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3021 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3060

Range 3: 2717 to 3056

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2717 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2776

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2777 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2836

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2837 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2896

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2897 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2956

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2957 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3016

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3017 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3056

Range 4: 1385 to 1645

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1385 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1444

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1445 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1504

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1505 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1564

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1565 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1624

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1625 TCTCGGGCAGCGTTGGGTCCT 1645

>Vector pSC2D, complete sequence

Sequence ID: LT727554.1 Length: 9962
Range 1: 3678 to 4087

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3678 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3737

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3738 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3797

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3798 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3857
Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511
||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3858 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3917

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3918 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3977

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3978 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 4037

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4038 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 4087

Range 2: 8121 to 8460

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8121 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 8180

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8181 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 8240

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 8241 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 8300

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 8301 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 8360

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 8361 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 8420

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 8421 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 8460

Range 3: 8117 to 8456

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8117 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 8176

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8177 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 8236

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8237 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 8296

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 8297 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 8356

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 8357 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 8416

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 8417 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 8456

Range 4: 4104 to 4350

Score:324 bits(359), Expect:4e-83,

Identities:217/247(88%), Gaps:30/247(12%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4104 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 4163

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4164 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 4223

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4224 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 4283

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 4284 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 4343

Query 1180 TCTCGGG 1186

|||||||
Sbjct 4344 TCTCGGG 4350

>Yeast expression vector pEX2, complete sequence

Sequence ID: LT727553.1 Length: 9214
Range 1: 2930 to 3339

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus
Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2930 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 2989

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2990 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3049

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3050 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3109

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3110 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3169

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3170 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3229

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3230 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3289

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3290 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3339

Range 2: 7373 to 7712

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7373 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 7432

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7433 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 7492

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 7493 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 7552

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 7553 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 7612

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 7613 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 7672

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 7673 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 7712

Range 3: 7369 to 7708

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7369 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 7428

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7429 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 7488

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7489 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 7548

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 7549 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 7608

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 7609 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 7668

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 7669 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 7708

Range 4: 3356 to 3602

Score:324 bits(359), Expect:4e-83,

Identities:217/247(88%), Gaps:30/247(12%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3356 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3415

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3416 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3475

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3476 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3535

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3536 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 3595

Query 1180 TCTCGGG 1186

 |||||||
Sbjct 3596 TCTCGGG 3602

>Yeast expression vector pEX0, complete sequence

Sequence ID: LT727552.1 Length: 7511
Range 1: 1227 to 1636

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1227 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1286

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1287 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1346

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1347 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1406

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1407 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1466

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1467 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1526

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1527 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1586

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1587 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1636

Range 2: 5670 to 6009

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5670 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 5729

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5730 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 5789

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 5790 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 5849

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 5850 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 5909

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 5910 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 5969

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 5970 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 6009

Range 3: 5666 to 6005

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5666 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 5725

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5726 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 5785

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5786 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 5845

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 5846 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 5905

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 5906 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 5965

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 5966 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 6005

Range 4: 1653 to 1899

Score:324 bits(359), Expect:4e-83,

Identities:217/247(88%), Gaps:30/247(12%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1653 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1712

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1713 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1772

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1773 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1832

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1833 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1892

Query 1180 TCTCGGG 1186

|||||||
Sbjct 1893 TCTCGGG 1899

>Vector pATHIL6HIFNG, complete sequence

Sequence ID: LT727534.1 Length: 6207
Range 1: 3307 to 3716

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3307 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3366

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3367 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3426

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3427 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3486

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3487 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3546

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3547 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3606

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3607 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3666

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3667 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3716

Range 2: 4366 to 4705

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4366 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 4425

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4426 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 4485

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 4486 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4545

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4546 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4605

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4606 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4665

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4666 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4705

Range 3: 4362 to 4701

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4362 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 4421

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4422 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 4481

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4482 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4541

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4542 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4601

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4602 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4661

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4662 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4701
Range 4: 3733 to 3993

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3733 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3792

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3793 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3852

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3853 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3912

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3913 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 3972

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 3973 TCTCGGGCAGCGTTGGGTCCT 3993

>Vector pATHIL6pA, complete sequence

Sequence ID: LT727532.1 Length: 5382
Range 1: 2484 to 2893

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2484 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 2543

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2544 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 2603

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 2604 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 2663

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 2664 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 2723

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 2724 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 2783

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2784 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 2843

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2844 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 2893

Range 2: 3543 to 3882

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3543 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3602

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3603 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3662

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3663 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3722

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3723 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3782

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3783 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3842

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3843 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3882

Range 3: 3539 to 3878

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3539 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3598

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3599 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3658

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3659 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3718

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

 |||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3719 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3778

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3779 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3838

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3839 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3878

Range 4: 2910 to 3170

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2910 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 2969

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2970 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3029

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3030 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3089

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3090 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 3149

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 3150 TCTCGGGCAGCGTTGGGTCCT 3170

>Vector pATHTNF, complete sequence

Sequence ID: LT727524.1 Length: 5310
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 1817 to 2156

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1817 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1876

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1877 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1936

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1937 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1996

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 1997 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2056

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2057 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2116

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2117 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2156

Range 3: 1813 to 2152

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

 || ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1813 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1872

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1873 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1932

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1933 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1992

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 1993 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2052

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2053 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2112

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2113 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2152

Range 4: 1184 to 1444

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444

>Vector pATMTNF1, complete sequence

Sequence ID: LT727523.1 Length: 5208
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 1817 to 2156

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1817 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1876

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1877 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1936

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1937 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1996

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 1997 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2056

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2057 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2116

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

 ||||||||||||||||||||||||||||||||||||||||
Sbjct 2117 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2156

Range 3: 1813 to 2152

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1813 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1872

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1873 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1932

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1933 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1992

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 1993 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2052

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2053 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2112

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2113 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2152

Range 4: 1184 to 1444

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423
Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200
|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444

>Vector pIDUHA, complete sequence

Sequence ID: LT727513.1 Length: 6065
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 2520 to 2859

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2520 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2579

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2580 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2639

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

 |||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2640 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2699

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2700 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2759

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2760 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2819

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2820 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2859

Range 3: 2516 to 2855

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2516 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2575

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2576 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2635

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2636 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2695

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2696 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2755

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2756 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2815

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2816 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2855

Range 4: 1184 to 1444

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243
Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079
||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444

>Vector pATSVTMx, complete sequence

Sequence ID: LT727509.1 Length: 6441
Range 1: 3541 to 3950

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3541 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3600

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3601 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3660

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3661 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3720

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3721 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3780

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3781 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3840

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3841 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3900

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3901 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3950

Range 2: 4600 to 4939

Score:438 bits(485), Expect:3e-117,
Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4600 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 4659

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4660 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 4719

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 4720 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4779

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4780 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4839

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4840 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4899

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4900 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4939

Range 3: 4596 to 4935

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4596 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 4655

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4656 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 4715

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4716 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4775

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4776 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4835

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4836 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4895

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4896 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4935
Range 4: 3967 to 4227

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3967 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 4026

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4027 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 4086

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4087 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 4146

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 4147 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 4206

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 4207 TCTCGGGCAGCGTTGGGTCCT 4227

>Vector pATMx, complete sequence

Sequence ID: LT727508.1 Length: 4112
Range 1: 1212 to 1621

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1212 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1271

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1272 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1331

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1332 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1391

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1392 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1451

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1452 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1511

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

 ||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1512 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1571

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1572 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1621

Range 2: 2271 to 2610

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2271 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2330

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2331 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2390

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2391 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2450

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2451 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2510

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2511 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2570

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2571 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2610

Range 3: 2267 to 2606

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2267 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2326

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2327 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2386

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2387 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2446
Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875
|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2447 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2506

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2507 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2566

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2567 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2606

Range 4: 1638 to 1898

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1638 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1697

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1698 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1757

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1758 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1817

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1818 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1877

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1878 TCTCGGGCAGCGTTGGGTCCT 1898

>Fungal expression vector pSal43A, complete sequence

Sequence ID: LT727506.1 Length: 5873
Range 1: 3357 to 3766

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3357 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3416

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3417 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3476

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

 ||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3477 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3536

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3537 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3596

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3597 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3656

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3657 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3716

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3717 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3766

Range 2: 4032 to 4371

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4032 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 4091

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4092 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 4151

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 4152 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4211

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4212 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4271

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4272 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4331

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4332 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4371

Range 3: 4028 to 4367

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus
Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720
|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4028 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 4087

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4088 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 4147

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4148 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4207

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4208 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4267

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4268 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4327

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4328 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4367

Range 4: 3783 to 4037

Score:334 bits(370), Expect:2e-86,

Identities:224/255(88%), Gaps:30/255(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3783 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3842

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3843 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3902

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3903 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3962

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3963 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 4022

Query 1180 TCTCGGGCAGCGTTG 1194

|||||||| ||||||
Sbjct 4023 TCTCGGGCCGCGTTG 4037

>Bacterial expression vector pET-11c, complete sequence

Sequence ID: LT727491.1 Length: 5675
Range 1: 2070 to 2479
Score:537 bits(595), Expect:4e-147,
Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2070 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 2129

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2130 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 2189

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 2190 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 2249

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 2250 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 2309

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 2310 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 2369

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2370 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 2429

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2430 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 2479

Range 2: 3834 to 4173

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3834 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3893

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3894 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3953

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3954 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4013

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4014 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4073

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4074 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4133
Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300
||||||||||||||||||||||||||||||||||||||||
Sbjct 4134 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4173

Range 3: 3830 to 4169

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3830 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3889

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3890 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3949

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3950 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4009

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4010 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4069

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4070 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4129

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4130 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4169

Range 4: 2496 to 2756

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2496 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 2555

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2556 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 2615

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2616 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 2675

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 2676 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2735
Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200
|||||||||||||||||||||
Sbjct 2736 TCTCGGGCAGCGTTGGGTCCT 2756

>Bacterial expression vector pET-11b, complete sequence

Sequence ID: LT727490.1 Length: 5676
Range 1: 2071 to 2480

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2071 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 2130

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2131 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 2190

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 2191 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 2250

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 2251 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 2310

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 2311 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 2370

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2371 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 2430

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2431 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 2480

Range 2: 3835 to 4174

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3835 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3894

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3895 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3954
Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160
|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3955 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4014

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4015 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4074

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4075 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4134

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4135 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4174

Range 3: 3831 to 4170

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3831 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3890

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3891 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3950

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3951 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4010

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4011 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4070

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4071 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4130

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4131 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4170

Range 4: 2497 to 2757

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2497 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 2556
Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079
||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2557 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 2616

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2617 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 2676

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 2677 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2736

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2737 TCTCGGGCAGCGTTGGGTCCT 2757

>Bacterial expression vector pET-11a, complete sequence

Sequence ID: LT727489.1 Length: 5677
Range 1: 2072 to 2481

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2072 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 2131

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2132 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 2191

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 2192 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 2251

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 2252 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 2311

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 2312 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 2371

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2372 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 2431

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2432 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 2481

Range 2: 3836 to 4175

Score:438 bits(485), Expect:3e-117,
Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3836 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3895

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3896 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3955

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3956 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4015

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4016 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4075

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4076 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4135

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4136 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4175

Range 3: 3832 to 4171

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3832 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3891

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3892 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3951

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3952 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4011

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4012 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4071

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4072 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4131

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4132 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4171
Range 4: 2498 to 2758

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2498 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 2557

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2558 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 2617

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2618 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 2677

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 2678 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2737

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2738 TCTCGGGCAGCGTTGGGTCCT 2758

>Bacterial expression vector pJBtetT7POL, complete sequence

Sequence ID: LT727466.1 Length: 13318
Range 1: 902 to 1311

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1311 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1252

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1251 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1192

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1191 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1132

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1131 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1072

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1071 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1012
Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611
||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1011 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 952

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 951 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 902

Range 2: 625 to 885

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 885 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 826

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 825 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 766

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 765 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 706

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 705 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 646

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 645 TCTCGGGCAGCGTTGGGTCCT 625

Range 3: 10327 to 10513

Score:257 bits(284), Expect:5e-63,

Identities:167/187(89%), Gaps:20/187(10%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 10327 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 10386

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 10387 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 10446

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 10447 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 10506

Query 462 GCGGCCG 468

|||||||
Sbjct 10507 GCGGCCG 10513
>Vector pT7POL23, complete sequence
Sequence ID: LT727456.1 Length: 11164
Range 1: 5605 to 6014

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5605 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 5664

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5665 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 5724

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 5725 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 5784

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 5785 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5844

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5845 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5904

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5905 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5964

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5965 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 6014

Range 2: 6031 to 6151

Score:178 bits(197), Expect:4e-39,

Identities:111/121(92%), Gaps:10/121(8%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6031 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 6090

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6091 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 6150

Query 1080 G 1080

|
Sbjct 6151 G 6151
>Cloning vector pKT240tet2, complete sequence
Sequence ID: LT727452.1 Length: 9277
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 1184 to 1444

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

 ||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444

>Bacterial expression vector pJBtrc, complete sequence

Sequence ID: LT727449.1 Length: 7952
Range 1: 902 to 1311

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1311 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1252

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1251 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1192

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1191 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1132

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1131 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1072

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1071 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1012

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1011 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 952

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 951 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 902

Range 2: 625 to 885

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 885 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 826

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 825 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 766

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 765 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 706

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 705 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 646

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 645 TCTCGGGCAGCGTTGGGTCCT 625

>Cloning vector pKT240tet1, complete sequence

Sequence ID: LT727446.1 Length: 12950
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 1184 to 1444

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus
Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023
||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444

>Bacterial expression vector pJB3tet2, complete sequence

Sequence ID: LT727434.1 Length: 6440
Range 1: 902 to 1311

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1311 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1252

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1251 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1192

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1191 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1132

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1131 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1072

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1071 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1012

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1011 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 952

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 951 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 902
Range 2: 625 to 885

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 885 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 826

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 825 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 766

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 765 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 706

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 705 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 646

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 645 TCTCGGGCAGCGTTGGGTCCT 625

>Bacterial expression vector pJB3tet1, complete sequence

Sequence ID: LT727433.1 Length: 6840
Range 1: 1302 to 1711

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1711 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1652

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1651 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1592

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1591 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1532

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1531 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1472

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1471 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1412

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

 ||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1411 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1352

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1351 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1302

Range 2: 1025 to 1285

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1285 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1226

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1225 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1166

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1165 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1106

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1105 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1046

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1045 TCTCGGGCAGCGTTGGGTCCT 1025

>Vector pLPPsOmpArPDI, complete sequence

Sequence ID: LT727428.1 Length: 8486
Range 1: 4887 to 5296

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4887 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 4946

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4947 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 5006

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 5007 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 5066

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 5067 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5126

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5127 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5186

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5187 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5246

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5247 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5296

Range 2: 6649 to 6988

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6649 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 6708

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6709 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 6768

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 6769 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 6828

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 6829 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 6888

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 6889 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 6948

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 6949 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 6988

Range 3: 6645 to 6984

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6645 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 6704

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

 |||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6705 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 6764

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6765 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 6824

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 6825 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 6884

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 6885 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 6944

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 6945 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 6984

Range 4: 5313 to 5573

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5313 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5372

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5373 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5432

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5433 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5492

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5493 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5552

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 5553 TCTCGGGCAGCGTTGGGTCCT 5573

>Vector pTRKH1, complete sequence

Sequence ID: LT727421.1 Length: 10948
Range 1: 7155 to 7564

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7155 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 7214

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7215 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 7274

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 7275 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 7334

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 7335 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 7394

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 7395 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 7454

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 7455 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 7514

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7515 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 7564

Range 2: 7581 to 7827

Score:324 bits(359), Expect:4e-83,

Identities:217/247(88%), Gaps:30/247(12%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7581 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 7640

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7641 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 7700

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7701 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 7760

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 7761 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 7820

Query 1180 TCTCGGG 1186

|||||||
Sbjct 7821 TCTCGGG 7827

Range 3: 5746 to 6054

Score:233 bits(258), Expect:6e-56,
Identities:242/317(76%), Gaps:39/317(12%), Strand: Plus/Plus

Query 680 CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----------CAAGTCAGA 729

||||||||||||||||||||||||||||| |||||||| || ||| ||||
Sbjct 5746 CGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT 5805

Query 730 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGG----------A 779

|||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5806 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCG 5865

Query 780 AGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG 839

||||||||||||||| || | | | ||||||| | | ||||| || | | ||
Sbjct 5866 TGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGG-TGTCATTCCGCTGTTATGGC--CGC 5922

Query 840 GCTT-TCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC 898

| || ||||| | ||||| | |||||||| | ||||| |||||||||||||
Sbjct 5923 GTTTGTCTCATT--CCACGC---CTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGC 5977

Query 899 TG----------ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC 948

|| ||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 5978 TGGACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATC 6037

Query 949 GTCTTGAGTCCAACACG 965

|||||||||||||| ||
Sbjct 6038 GTCTTGAGTCCAACCCG 6054

Range 4: 5746 to 6058

Score:224 bits(248), Expect:3e-53,

Identities:242/319(76%), Gaps:35/319(10%), Strand: Plus/Plus

Query 11 CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC---------- 60
||||||||||||||||||||||||||||| |||||||| ||| |||||
Sbjct 5746 CGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT 5805

Query 61 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG 120

||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct 5806 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCG 5865

Query 121 TG-----TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGC 175

|| | | | ||||| ||| | | |||| || | || | | || || ||
Sbjct 5866 TGCGCTCTCCTGTTCCTGC--CTTTCGGTTTACCGGT-GTCATTCCGCTGTTATGGCCGC 5922

Query 176 G--TGGCTGCT--CACGCTGTACCTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTG 231

| || || | ||||| || |||||||| | ||||| |||||||||||||||
Sbjct 5923 GTTTGTCTCATTCCACGCCTGAC---ACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTG 5979

Query 232 GGCTGTGTG----------CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGT 281

| |||| || |||||||| ||||||||||||||||||||||||||||||||
Sbjct 5980 GACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGT 6039

Query 282 CTTGAGTCCAACCCGGTAA 300

|||||||||||||||| ||
Sbjct 6040 CTTGAGTCCAACCCGGAAA 6058
>Vector pBRgHIL61, complete sequence
Sequence ID: LT727419.1 Length: 9484
Range 1: 5463 to 5872

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5463 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 5522

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5523 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 5582

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 5583 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 5642

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 5643 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5702

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5703 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5762

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5763 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5822

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5823 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5872

Range 2: 4373 to 4710

Score:448 bits(496), Expect:2e-120,

Identities:298/338(88%), Gaps:40/338(11%), Strand: Plus/Minus

Query 364 GGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACT--- 420

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4710 GGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGT 4651

Query 421 -------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCG 473

|||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4650 CCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCG 4591

Query 474 CTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATT 523

||||||| |||||||||||||||||||||||||||||||||||||||||||
Sbjct 4590 CTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATT 4531

Query 524 CTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTA 573

 |||||||||||||||| ||||||||||||||||||||||||||||||||||
Sbjct 4530 CTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTA 4471

Query 574 GATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACCAGCCTAACTTCG 623

||||||||||||||||||||||||||| |||||||||||||||||||||||
Sbjct 4470 GATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCG 4411

Query 624 ATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||
Sbjct 4410 ATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 4373

Range 3: 7225 to 7564

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7225 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 7284

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7285 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 7344

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 7345 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 7404

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 7405 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 7464

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 7465 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 7524

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 7525 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 7564

Range 4: 7221 to 7560

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7221 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 7280

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7281 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 7340
Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825
||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7341 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 7400

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 7401 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 7460

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 7461 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 7520

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 7521 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 7560

Range 5: 5889 to 6149

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5889 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5948

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5949 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 6008

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6009 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 6068

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 6069 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 6128

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 6129 TCTCGGGCAGCGTTGGGTCCT 6149

Range 6: 4236 to 4356

Score:178 bits(197), Expect:4e-39,

Identities:111/121(92%), Gaps:10/121(8%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4356 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 4297

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4296 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 4237
Query 1080 G 1080
|
Sbjct 4236 G 4236

>Bacterial expression vector pPLc236, complete sequence

Sequence ID: LT727393.1 Length: 4425
Range 1: 418 to 827

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 418 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 477

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 478 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 537

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 538 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 597

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 598 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 657

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 658 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 717

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 718 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 777

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 778 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 827

Range 2: 2180 to 2519

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2180 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 2239

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2240 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 2299
Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160
|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 2300 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 2359

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 2360 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2419

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2420 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2479

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2480 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2519

Range 3: 2176 to 2515

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2176 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 2235

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2236 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 2295

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2296 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 2355

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 2356 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2415

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2416 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2475

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2476 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2515

Range 4: 844 to 1104

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 844 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 903
Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079
||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 904 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 963

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 964 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1023

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1024 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1083

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1084 TCTCGGGCAGCGTTGGGTCCT 1104

>Vector pATmIL6hIFNG, complete sequence

Sequence ID: LT727371.1 Length: 5856
Range 1: 2658 to 3067

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2658 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 2717

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2718 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 2777

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 2778 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 2837

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 2838 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 2897

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 2898 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 2957

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2958 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3017

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3018 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3067

Range 2: 3717 to 4056

Score:438 bits(485), Expect:3e-117,
Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3717 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3776

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3777 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3836

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3837 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3896

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3897 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3956

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3957 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4016

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4017 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4056

Range 3: 3713 to 4052

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3713 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3772

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3773 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3832

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3833 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3892

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3893 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3952

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3953 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4012

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4013 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4052
Range 4: 3084 to 3344

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3084 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3143

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3144 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3203

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3204 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3263

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3264 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 3323

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 3324 TCTCGGGCAGCGTTGGGTCCT 3344

>Bacterial expression vector pJBpn25lacZ, complete sequence

Sequence ID: LT727366.1 Length: 12509
Range 1: 902 to 1311

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1311 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1252

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1251 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1192

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1191 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1132

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1131 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1072

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1071 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1012
Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611
||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1011 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 952

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 951 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 902

Range 2: 625 to 885

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 885 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 826

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 825 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 766

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 765 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 706

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 705 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 646

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 645 TCTCGGGCAGCGTTGGGTCCT 625

>Bacterial expression vector pJBtrc10, complete sequence

Sequence ID: LT727349.1 Length: 8463
Range 1: 902 to 1311

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1311 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1252

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1251 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1192

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1191 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1132

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

 ||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1131 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1072

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1071 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1012

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1011 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 952

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 951 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 902

Range 2: 625 to 885

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 885 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 826

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 825 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 766

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 765 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 706

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 705 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 646

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 645 TCTCGGGCAGCGTTGGGTCCT 625

>Bacterial expression vector pJBtrc2, complete sequence

Sequence ID: LT727348.1 Length: 7718
Range 1: 902 to 1311

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1311 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 1252

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1251 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 1192

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 1191 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 1132

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 1131 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 1072

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 1071 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1012

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1011 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 952

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 951 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 902

Range 2: 625 to 885

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 885 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 826

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 825 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 766

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 765 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 706

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 705 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 646

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 645 TCTCGGGCAGCGTTGGGTCCT 625

>Mammalian expression vector pACCMVpLpA, complete sequence

Sequence ID: LT727333.1 Length: 8796
Range 1: 5889 to 6298

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus
Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5889 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 5948

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5949 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 6008

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 6009 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 6068

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 6069 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 6128

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 6129 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 6188

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 6189 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 6248

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6249 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 6298

Range 2: 6948 to 7287

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6948 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 7007

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7008 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 7067

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 7068 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 7127

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 7128 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 7187

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 7188 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 7247

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 7248 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 7287

Range 3: 6944 to 7283

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6944 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 7003

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7004 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 7063

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7064 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 7123

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 7124 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 7183

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 7184 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 7243

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 7244 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 7283

Range 4: 6315 to 6575

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6315 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 6374

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6375 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 6434

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6435 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 6494

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 6495 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 6554

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

 |||||||||||||||||||||
Sbjct 6555 TCTCGGGCAGCGTTGGGTCCT 6575

>Bacterial expression vector pJE202, complete sequence

Sequence ID: LT727306.1 Length: 13009
Range 1: 9406 to 9815

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9406 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 9465

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9466 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 9525

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 9526 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 9585

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 9586 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 9645

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 9646 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 9705

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 9706 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 9765

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9766 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 9815

Range 2: 11168 to 11507

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11168 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 11227

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11228 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 11287

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 11288 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 11347

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 11348 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 11407

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 11408 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 11467

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 11468 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 11507

Range 3: 11164 to 11503

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11164 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 11223

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11224 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 11283

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11284 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 11343

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 11344 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 11403

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 11404 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 11463

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 11464 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 11503

Range 4: 9832 to 10092

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9832 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 9891

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

 ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9892 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 9951

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 9952 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 10011

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 10012 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 10071

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 10072 TCTCGGGCAGCGTTGGGTCCT 10092

>Vector pTRKH3, complete sequence

Sequence ID: LT727213.1 Length: 7766
Range 1: 7097 to 7506

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7097 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 7156

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7157 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 7216

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 7217 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 7276

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 7277 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 7336

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 7337 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 7396

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 7397 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 7456

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7457 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 7506

Range 2: 7523 to 7766

Score:319 bits(353), Expect:2e-81,

Identities:214/244(88%), Gaps:30/244(12%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7523 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 7582

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7583 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 7642

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7643 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 7702

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 7703 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 7762

Query 1180 TCTC 1183

||||
Sbjct 7763 TCTC 7766

Range 3: 5688 to 5996

Score:233 bits(258), Expect:6e-56,

Identities:242/317(76%), Gaps:39/317(12%), Strand: Plus/Plus

Query 680 CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----------CAAGTCAGA 729

||||||||||||||||||||||||||||| |||||||| || ||| ||||
Sbjct 5688 CGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT 5747

Query 730 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGG----------A 779

|||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5748 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCG 5807

Query 780 AGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG 839

||||||||||||||| || | | | ||||||| | | ||||| || | | ||
Sbjct 5808 TGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGG-TGTCATTCCGCTGTTATGGC--CGC 5864

Query 840 GCTT-TCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC 898

| || ||||| | ||||| | |||||||| | ||||| |||||||||||||
Sbjct 5865 GTTTGTCTCATT--CCACGC---CTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGC 5919

Query 899 TG----------ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC 948

|| ||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 5920 TGGACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATC 5979

Query 949 GTCTTGAGTCCAACACG 965

|||||||||||||| ||
Sbjct 5980 GTCTTGAGTCCAACCCG 5996

Range 4: 5688 to 6000

Score:224 bits(248), Expect:3e-53,
Identities:242/319(76%), Gaps:35/319(10%), Strand: Plus/Plus

Query 11 CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC---------- 60
||||||||||||||||||||||||||||| |||||||| ||| |||||
Sbjct 5688 CGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT 5747

Query 61 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG 120

||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct 5748 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCG 5807

Query 121 TG-----TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGC 175

|| | | | ||||| ||| | | |||| || | || | | || || ||
Sbjct 5808 TGCGCTCTCCTGTTCCTGC--CTTTCGGTTTACCGGT-GTCATTCCGCTGTTATGGCCGC 5864

Query 176 G--TGGCTGCT--CACGCTGTACCTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTG 231

| || || | ||||| || |||||||| | ||||| |||||||||||||||
Sbjct 5865 GTTTGTCTCATTCCACGCCTGAC---ACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTG 5921

Query 232 GGCTGTGTG----------CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGT 281

| |||| || |||||||| ||||||||||||||||||||||||||||||||
Sbjct 5922 GACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGT 5981

Query 282 CTTGAGTCCAACCCGGTAA 300

|||||||||||||||| ||
Sbjct 5982 CTTGAGTCCAACCCGGAAA 6000

>Vector pMC1403, complete sequence

Sequence ID: LT727182.1 Length: 9886
Range 1: 6283 to 6692

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6283 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 6342

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6343 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 6402

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 6403 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 6462

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 6463 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 6522

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 6523 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 6582

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 6583 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 6642

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6643 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 6692

Range 2: 8045 to 8384

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8045 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 8104

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8105 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 8164

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 8165 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 8224

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 8225 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 8284

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 8285 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 8344

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 8345 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 8384

Range 3: 8041 to 8380

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8041 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 8100

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8101 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 8160

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8161 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 8220

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

 |||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 8221 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 8280

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 8281 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 8340

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 8341 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 8380

Range 4: 6709 to 6969

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6709 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 6768

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6769 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 6828

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6829 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 6888

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 6889 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 6948

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 6949 TCTCGGGCAGCGTTGGGTCCT 6969

>Vector pAT153MIL2F2, complete sequence

Sequence ID: LT727177.1 Length: 4664
Range 1: 758 to 1167

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 758 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 817

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 818 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 877

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 878 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 937

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 938 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 997

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 1057

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 1058 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 1117

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1118 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 1167

Range 2: 1817 to 2156

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1817 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1876

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1877 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1936

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1937 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1996

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 1997 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 2056

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 2057 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 2116

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 2117 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 2156

Range 3: 1813 to 2152

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

 || ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1813 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1872

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1873 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1932

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1933 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1992

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 1993 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 2052

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 2053 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 2112

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 2113 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 2152

Range 4: 1184 to 1444

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1184 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 1243

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1244 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1303

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1304 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1363

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 1364 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1423

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 1424 TCTCGGGCAGCGTTGGGTCCT 1444

>Vector pSEVA512S-16S-pad, complete sequence

Sequence ID: ON366567.1 Length: 5513
Range 1: 4219 to 4628

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4219 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 4278

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4279 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 4338

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 4339 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 4398

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 4399 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 4458

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 4459 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 4518

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 4519 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 4578

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4579 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 4628

Range 2: 4645 to 4737

Score:128 bits(141), Expect:7e-24,

Identities:83/93(89%), Gaps:10/93(10%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCC---------- 1019

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4645 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 4704

Query 1020 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGA 1052

|||||||||||||||||||||||||||||||||
Sbjct 4705 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGA 4737

>Synthetic construct His-TEV-Nsp15 gene, complete cds

Sequence ID: ON191567.1 Length: 6760
Range 1: 4728 to 5137

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5137 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 5078
Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411
|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5077 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 5018

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 5017 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 4958

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 4957 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 4898

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 4897 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 4838

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 4837 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 4778

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4777 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 4728

Range 2: 3034 to 3373

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3373 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3314

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3313 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3254

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3253 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3194

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3193 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3134

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3133 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3074

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3073 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3034
Range 3: 3038 to 3377

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3377 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3318

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3317 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3258

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3257 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3198

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3197 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3138

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3137 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3078

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3077 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3038

Range 4: 4451 to 4711

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4711 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 4652

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4651 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 4592

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4591 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 4532

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 4531 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 4472

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 4471 TCTCGGGCAGCGTTGGGTCCT 4451
>Cloning vector pET-RA-Y, complete sequence
Sequence ID: OK655938.1 Length: 6817
Range 1: 6030 to 6439

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6439 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 6380

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6379 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 6320

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 6319 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 6260

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 6259 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 6200

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 6199 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 6140

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 6139 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 6080

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6079 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 6030

Range 2: 4108 to 4447

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4447 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 4388

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4387 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 4328

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 4327 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4268

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4267 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4208

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4207 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4148

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4147 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4108

Range 3: 4112 to 4451

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4451 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 4392

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4391 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 4332

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4331 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4272

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4271 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4212

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4211 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4152

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4151 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4112

Range 4: 5768 to 6013

Score:323 bits(357), Expect:2e-82,

Identities:216/246(88%), Gaps:30/246(12%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6013 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5954

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5953 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5894

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

 | |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5893 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5834

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5833 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5774

Query 1180 TCTCGG 1185

||||||
Sbjct 5773 TCTCGG 5768

>Expression vector p15Tc-litR, complete sequence

Sequence ID: MZ683493.1 Length: 5337
Range 1: 2683 to 3092

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2683 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 2742

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 2743 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 2802

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 2803 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 2862

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 2863 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 2922

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 2923 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 2982

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 2983 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3042

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3043 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3092

Range 2: 3109 to 3369

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3109 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3168

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3169 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3228

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3229 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3288

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3289 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 3348

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 3349 TCTCGGGCAGCGTTGGGTCCT 3369

Range 3: 1274 to 1582

Score:233 bits(258), Expect:6e-56,

Identities:242/317(76%), Gaps:39/317(12%), Strand: Plus/Plus

Query 680 CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----------CAAGTCAGA 729

||||||||||||||||||||||||||||| |||||||| || ||| ||||
Sbjct 1274 CGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT 1333

Query 730 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGG----------A 779

|||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1334 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCG 1393

Query 780 AGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG 839

||||||||||||||| || | | | ||||||| | | ||||| || | | ||
Sbjct 1394 TGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGG-TGTCATTCCGCTGTTATGGC--CGC 1450

Query 840 GCTT-TCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC 898

| || ||||| | ||||| | |||||||| | ||||| |||||||||||||
Sbjct 1451 GTTTGTCTCATT--CCACGC---CTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGC 1505

Query 899 TG----------ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC 948

|| ||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 1506 TGGACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATC 1565

Query 949 GTCTTGAGTCCAACACG 965

|||||||||||||| ||
Sbjct 1566 GTCTTGAGTCCAACCCG 1582

Range 4: 1274 to 1586

Score:224 bits(248), Expect:3e-53,

Identities:242/319(76%), Gaps:35/319(10%), Strand: Plus/Plus

Query 11 CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC---------- 60
 ||||||||||||||||||||||||||||| |||||||| ||| |||||
Sbjct 1274 CGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT 1333

Query 61 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG 120

||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct 1334 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCG 1393

Query 121 TG-----TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGC 175

|| | | | ||||| ||| | | |||| || | || | | || || ||
Sbjct 1394 TGCGCTCTCCTGTTCCTGC--CTTTCGGTTTACCGGT-GTCATTCCGCTGTTATGGCCGC 1450

Query 176 G--TGGCTGCT--CACGCTGTACCTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTG 231

| || || | ||||| || |||||||| | ||||| |||||||||||||||
Sbjct 1451 GTTTGTCTCATTCCACGCCTGAC---ACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTG 1507

Query 232 GGCTGTGTG----------CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGT 281

| |||| || |||||||| ||||||||||||||||||||||||||||||||
Sbjct 1508 GACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGT 1567

Query 282 CTTGAGTCCAACCCGGTAA 300

|||||||||||||||| ||
Sbjct 1568 CTTGAGTCCAACCCGGAAA 1586

>Synthetic construct Golden Gate selectable marker insert TetR sequence

Sequence ID: OK165508.1 Length: 1881
Range 1: 355 to 764

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 764 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 705

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 704 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 645

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 644 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 585

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 584 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 525

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 524 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 465

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 464 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 405

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 404 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 355

Range 2: 144 to 338

Score:263 bits(291), Expect:1e-64,

Identities:175/197(89%), Gaps:22/197(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 338 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 279

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 278 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 219

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 218 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 159

Query 1130 CCAACCCTTGGCCATCG 1146

|||||||||| |||||
Sbjct 158 CCAACCCTTG--CATCG 144

>Cloning vector pRK177, complete sequence

Sequence ID: MN626476.1 Length: 11938
Range 1: 4956 to 5365

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4956 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 5015

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5016 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 5075

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 5076 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 5135

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 5136 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5195

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5196 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5255

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5256 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5315
Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5316 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5365

Range 2: 6085 to 6424

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6424 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 6365

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6364 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 6305

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| |||||||||||||||||||||||||| |||||||||||||
Sbjct 6304 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATGCCTGTCCGCCTTT 6245

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 6244 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 6185

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 6184 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 6125

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 6124 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 6085

Range 3: 6089 to 6431

Score:430 bits(476), Expect:4e-115,

Identities:297/343(87%), Gaps:40/343(11%), Strand: Plus/Minus

Query 663 CATGGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA-- 720

|| || ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6431 CACGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAA 6372

Query 721 --------CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCC 772

||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6371 TCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCC 6312

Query 773 CCCTGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTC 822

|||||||| ||||||||||||||||||||||||||||||||||| ||||||
Sbjct 6311 CCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATGCCTGTC 6252

Query 823 CGCCTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAG 872

||||||||||||||||| ||||||||| ||||||||||||||||||||||
Sbjct 6251 CGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAG 6192

Query 873 TTCGGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGA 922

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 6191 TTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGA 6132

Query 923 CCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

|||||||||||||||||||||||||||||||||||||||| ||
Sbjct 6131 CCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 6089

Range 4: 5382 to 5474

Score:128 bits(141), Expect:7e-24,

Identities:83/93(89%), Gaps:10/93(10%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCC---------- 1019

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5382 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5441

Query 1020 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGA 1052

|||||||||||||||||||||||||||||||||
Sbjct 5442 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGA 5474

>Vector pKW20_CDFtet_pAraRedCas9_TracrRNA, complete sequence

Sequence ID: MN927219.1 Length: 10272
Range 1: 8291 to 8700

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8700 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 8641

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8640 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 8581

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 8580 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 8521

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 8520 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 8461

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 8460 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 8401

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 8400 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 8341
Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8340 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 8291

Range 2: 9706 to 9968

Score:212 bits(234), Expect:2e-49,

Identities:206/265(78%), Gaps:34/265(12%), Strand: Plus/Plus

Query 733 GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGG----------AAGC 782

||||||||||||||||||| ||||||| || ||||||| | || ||
Sbjct 9706 GGCGAAACCCGACAGGACT-TAAAGATCCCCACCGTTTCCGGCGGGTCGCTCCCTCTTGC 9764

Query 783 GCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTG-TCCGCCTTTCTCCCTT-CGG- 839

||||||||||||||||||||||| |||||||||||||| |||||||||||||||| |||
Sbjct 9765 GCTCTCCTGTTCCGACCCTGCCGTTTACCGGATACCTGTTCCGCCTTTCTCCCTTACGGG 9824

Query 840 ---------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCG 890

||||||||| |||||| | | |||||||| | |||||||||||||||||
Sbjct 9825 AAGTGTGGCGCTTTCTCATAGCTCACACACT-GGTATCTCGGCTCGGTGTAGGTCGTTCG 9883

Query 891 CTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGG 940

|||||||||| | |||| |||||||||||||||| ||||||||||||||||
Sbjct 9884 CTCCAAGCTGGGCTGTAAGCAAGAACTCCCCGTTCAGCCCGACTGCTGCGCCTTATCCGG 9943

Query 941 TAACTATCGTCTTGAGTCCAACACG 965

||||| | |||||||||||| ||
Sbjct 9944 TAACTGTTCACTTGAGTCCAACCCG 9968

Range 3: 9706 to 9974

Score:210 bits(232), Expect:7e-49,

Identities:210/270(78%), Gaps:32/270(11%), Strand: Plus/Plus

Query 64 GGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTG- 122

||||||||||||||||||| ||||||| || ||||||| | || |||||||| ||
Sbjct 9706 GGCGAAACCCGACAGGACT-TAAAGATCCCCACCGTTTCCGGCGGGTCGCTCCCTCTTGC 9764

Query 123 ---------TTCCGACCCTGCCGCTTACCGGATACCTGT-CCGCCTTTCTCCCTT-CGGG 171

|||||||||||||| ||||||||||||||| ||||||||||||||| ||||
Sbjct 9765 GCTCTCCTGTTCCGACCCTGCCGTTTACCGGATACCTGTTCCGCCTTTCTCCCTTACGGG 9824

Query 172 AAGCGTGGC---TGCTCAC-GCTGTACC-----TATCTCAGTTCGGTGTAGGTCGTTCGC 222

||| ||||| | |||| ||| | |||||| | ||||||||||||||||||
Sbjct 9825 AAGTGTGGCGCTTTCTCATAGCTCACACACTGGTATCTCGGCTCGGTGTAGGTCGTTCGC 9884

Query 223 TCCAAGCTGGGCTGTGTGC----------CGTTCAGCCCGACCGCTGCGCCTTATCCGGT 272

||||||||||||||| || ||||||||||||| |||||||||||||||||
Sbjct 9885 TCCAAGCTGGGCTGTAAGCAAGAACTCCCCGTTCAGCCCGACTGCTGCGCCTTATCCGGT 9944

Query 273 AACTATCGTCTTGAGTCCAACCCGGTAAAG 302

|||| | |||||||||||||||| ||||
Sbjct 9945 AACTGTTCACTTGAGTCCAACCCGGAAAAG 9974

Range 4: 8182 to 8274

Score:128 bits(141), Expect:7e-24,

Identities:83/93(89%), Gaps:10/93(10%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCC---------- 1019

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 8274 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 8215

Query 1020 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGA 1052

|||||||||||||||||||||||||||||||||
Sbjct 8214 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGA 8182

>Cloning vector pET14b_17AECD2C_StarStuff-L-PhaC, complete sequence

Sequence ID: MW151040.1 Length: 7869
Range 1: 3197 to 3606

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3606 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3547

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3546 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3487

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3486 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3427

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3426 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3367

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3366 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3307

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3306 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3247

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3246 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3197
Range 2: 1503 to 1842

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1842 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1783

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1782 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1723

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1722 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1663

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 1662 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 1603

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1602 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 1543

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 1542 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 1503

Range 3: 1507 to 1846

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1846 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1787

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1786 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1727

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1726 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1667

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 1666 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 1607

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1606 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 1547

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 1546 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 1507

Range 4: 2920 to 3180

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3180 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3121

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3120 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3061

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3060 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3001

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3000 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2941

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2940 TCTCGGGCAGCGTTGGGTCCT 2920

>Cloning vector pET14b_17AECD3C_Pipsqueaks-L-PhaC, complete sequence

Sequence ID: MW151039.1 Length: 7896
Range 1: 3197 to 3606

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3606 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3547

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3546 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3487

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3486 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3427

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3426 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3367

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3366 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3307
Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611
||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3306 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3247

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3246 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3197

Range 2: 1503 to 1842

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1842 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1783

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1782 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1723

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1722 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1663

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 1662 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 1603

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1602 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 1543

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 1542 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 1503

Range 3: 1507 to 1846

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1846 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1787

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1786 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1727

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1726 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1667

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 1666 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 1607

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1606 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 1547

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 1546 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 1507

Range 4: 2920 to 3180

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3180 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3121

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3120 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3061

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3060 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3001

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3000 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2941

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2940 TCTCGGGCAGCGTTGGGTCCT 2920

>Cloning vector pET14b_17AECD5C_JAWS-L-PhaC, complete sequence

Sequence ID: MW151038.1 Length: 7260
Range 1: 3197 to 3606

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3606 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3547

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3546 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3487
Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461
||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3486 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3427

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3426 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3367

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3366 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3307

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3306 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3247

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3246 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3197

Range 2: 1503 to 1842

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1842 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1783

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1782 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1723

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1722 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1663

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 1662 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 1603

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1602 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 1543

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 1542 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 1503

Range 3: 1507 to 1846

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1846 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1787

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1786 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1727

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1726 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1667

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 1666 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 1607

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1606 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 1547

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 1546 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 1507

Range 4: 2920 to 3180

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3180 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3121

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3120 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3061

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3060 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3001

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3000 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2941

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2940 TCTCGGGCAGCGTTGGGTCCT 2920

>Cloning vector pET14b_17AECD6C_Inca-L-PhaC, complete sequence

Sequence ID: MW151037.1 Length: 7245
Range 1: 3197 to 3606

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3606 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3547

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3546 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3487

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3486 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3427

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3426 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3367

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3366 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3307

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3306 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3247

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3246 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3197

Range 2: 1503 to 1842

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1842 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1783

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1782 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1723

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1722 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1663

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 1662 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 1603

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1602 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 1543

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 1542 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 1503

Range 3: 1507 to 1846

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1846 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1787

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1786 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1727

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1726 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1667

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 1666 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 1607

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1606 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 1547

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 1546 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 1507

Range 4: 2920 to 3180

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3180 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3121

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3120 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3061

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3060 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3001

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

 ||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3000 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2941

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2940 TCTCGGGCAGCGTTGGGTCCT 2920

>Cloning vector pET14b_17AECEBC_Giles-L-PhaC, complete sequence

Sequence ID: MW151036.1 Length: 7623
Range 1: 3197 to 3606

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3606 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3547

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3546 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3487

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3486 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3427

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3426 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3367

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3366 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3307

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3306 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3247

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3246 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3197

Range 2: 1503 to 1842

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1842 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1783

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1782 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1723

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1722 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1663

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 1662 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 1603

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1602 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 1543

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 1542 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 1503

Range 3: 1507 to 1846

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1846 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1787

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1786 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1727

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1726 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1667

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 1666 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 1607

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1606 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 1547

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 1546 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 1507

Range 4: 2920 to 3180

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

 ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3180 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3121

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3120 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3061

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3060 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3001

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3000 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2941

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2940 TCTCGGGCAGCGTTGGGTCCT 2920

>Cloning vector pET14b_17AECD7C_Dylan-L-PhaC, complete sequence

Sequence ID: MW151035.1 Length: 7428
Range 1: 3197 to 3606

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3606 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3547

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3546 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3487

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3486 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3427

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3426 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3367

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3366 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3307

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3306 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3247

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3246 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3197
Range 2: 1503 to 1842

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1842 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1783

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1782 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1723

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1722 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1663

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 1662 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 1603

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1602 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 1543

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 1542 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 1503

Range 3: 1507 to 1846

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1846 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1787

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1786 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1727

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1726 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1667

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 1666 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 1607

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1606 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 1547

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

 ||||||||||||||||||||||||||||||||||||| ||
Sbjct 1546 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 1507

Range 4: 2920 to 3180

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3180 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3121

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3120 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3061

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3060 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3001

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3000 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2941

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2940 TCTCGGGCAGCGTTGGGTCCT 2920

>Cloning vector pET14b_17AECD4C_D29-L-PhaC, complete sequence

Sequence ID: MW151034.1 Length: 7152
Range 1: 3197 to 3606

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3606 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3547

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3546 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3487

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3486 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3427

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3426 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3367

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3366 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3307

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3306 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3247

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3246 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3197

Range 2: 1503 to 1842

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1842 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1783

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1782 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1723

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1722 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1663

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 1662 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 1603

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1602 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 1543

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 1542 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 1503

Range 3: 1507 to 1846

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1846 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1787

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1786 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1727

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

 ||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1726 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1667

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 1666 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 1607

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1606 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 1547

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 1546 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 1507

Range 4: 2920 to 3180

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3180 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3121

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3120 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3061

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3060 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3001

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3000 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2941

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2940 TCTCGGGCAGCGTTGGGTCCT 2920

>Cloning vector pET14b_17AECEAC_Bongo-L-PhaC, complete sequence

Sequence ID: MW151033.1 Length: 7359
Range 1: 3197 to 3606

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3606 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 3547

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3546 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 3487

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 3486 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 3427

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 3426 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 3367

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 3366 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 3307

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 3306 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 3247

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3246 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 3197

Range 2: 1503 to 1842

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1842 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 1783

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1782 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 1723

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 1722 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 1663

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 1662 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 1603

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 1602 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 1543

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 1542 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 1503

Range 3: 1507 to 1846

Score:434 bits(480), Expect:3e-116,
Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1846 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 1787

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1786 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 1727

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 1726 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 1667

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 1666 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 1607

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 1606 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 1547

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 1546 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 1507

Range 4: 2920 to 3180

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3180 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 3121

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3120 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 3061

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3060 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 3001

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 3000 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 2941

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 2940 TCTCGGGCAGCGTTGGGTCCT 2920

>Cloning vector pC15-plsg, complete sequence

Sequence ID: MK353498.2 Length: 15280
Range 1: 11764 to 12173

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Plus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11764 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 11823

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 11824 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 11883

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 11884 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 11943

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 11944 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 12003

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 12004 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 12063

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 12064 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 12123

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 12124 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 12173

Range 2: 12190 to 12450

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Plus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 12190 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 12249

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 12250 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 12309

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 12310 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 12369

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 12370 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 12429

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

 |||||||||||||||||||||
Sbjct 12430 TCTCGGGCAGCGTTGGGTCCT 12450

Range 3: 14600 to 14908

Score:233 bits(258), Expect:6e-56,

Identities:242/317(76%), Gaps:39/317(12%), Strand: Plus/Plus

Query 680 CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----------CAAGTCAGA 729

||||||||||||||||||||||||||||| |||||||| || ||| ||||
Sbjct 14600 CGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT 14659

Query 730 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGG----------A 779

|||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 14660 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCG 14719

Query 780 AGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG 839

||||||||||||||| || | | | ||||||| | | ||||| || | | ||
Sbjct 14720 TGCGCTCTCCTGTTCCTGCCTTTCGGTTTACCGG-TGTCATTCCGCTGTTATGGC--CGC 14776

Query 840 GCTT-TCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGC 898

| || ||||| | ||||| | |||||||| | ||||| |||||||||||||
Sbjct 14777 GTTTGTCTCATT--CCACGC---CTGACACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGC 14831

Query 899 TG----------ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC 948

|| ||||||||||||||||| ||||||||||||||||||||||||||||||
Sbjct 14832 TGGACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATC 14891

Query 949 GTCTTGAGTCCAACACG 965

|||||||||||||| ||
Sbjct 14892 GTCTTGAGTCCAACCCG 14908

Range 4: 14600 to 14912

Score:224 bits(248), Expect:3e-53,

Identities:242/319(76%), Gaps:35/319(10%), Strand: Plus/Plus

Query 11 CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC---------- 60
||||||||||||||||||||||||||||| |||||||| ||| |||||
Sbjct 14600 CGTTTTTCCATAGGCTCCGCCCCCCTGACAAGCATCACGAAATCTGACGCTCAAATCAGT 14659

Query 61 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG 120

||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
Sbjct 14660 GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGCGGCTCCCTCG 14719

Query 121 TG-----TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGC 175

|| | | | ||||| ||| | | |||| || | || | | || || ||
Sbjct 14720 TGCGCTCTCCTGTTCCTGC--CTTTCGGTTTACCGGT-GTCATTCCGCTGTTATGGCCGC 14776

Query 176 G--TGGCTGCT--CACGCTGTACCTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTG 231

| || || | ||||| || |||||||| | ||||| |||||||||||||||
Sbjct 14777 GTTTGTCTCATTCCACGCCTGAC---ACTCAGTTCCGGGTAGGCAGTTCGCTCCAAGCTG 14833
Query 232 GGCTGTGTG----------CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGT 281
| |||| || |||||||| ||||||||||||||||||||||||||||||||
Sbjct 14834 GACTGTATGCACGAACCCCCCGTTCAGTCCGACCGCTGCGCCTTATCCGGTAACTATCGT 14893

Query 282 CTTGAGTCCAACCCGGTAA 300

|||||||||||||||| ||
Sbjct 14894 CTTGAGTCCAACCCGGAAA 14912

>Vector pET19b_ShHTL7cpGFP(L166)LSSmOrange, complete sequence

Sequence ID: MT270503.1 Length: 7892
Range 1: 5841 to 6250

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6250 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 6191

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6190 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 6131

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 6130 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 6071

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 6070 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 6011

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 6010 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5951

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5950 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5891

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5890 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5841

Range 2: 4147 to 4486

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4486 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 4427

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

 ||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4426 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 4367

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 4366 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4307

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4306 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4247

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4246 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4187

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4186 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4147

Range 3: 4151 to 4490

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4490 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 4431

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4430 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 4371

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4370 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4311

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4310 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4251

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4250 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4191

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4190 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4151

Range 4: 5564 to 5824

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus
Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023
||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5824 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5765

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5764 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5705

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5704 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5645

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5644 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5585

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 5584 TCTCGGGCAGCGTTGGGTCCT 5564

>Vector pET19b_HisDAD2cpGFP(G166), complete sequence

Sequence ID: MT270502.1 Length: 7175
Range 1: 5454 to 5863

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5863 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 5804

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5803 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 5744

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 5743 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 5684

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 5683 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5624

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5623 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5564

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5563 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5504

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5503 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5454
Range 2: 3760 to 4099

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4099 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 4040

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4039 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3980

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3979 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3920

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3919 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3860

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3859 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3800

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3799 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3760

Range 3: 3764 to 4103

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4103 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 4044

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4043 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3984

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3983 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3924

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3923 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3864

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3863 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3804
Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965
||||||||||||||||||||||||||||||||||||| ||
Sbjct 3803 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3764

Range 4: 5177 to 5437

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5437 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5378

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5377 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5318

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5317 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5258

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5257 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5198

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 5197 TCTCGGGCAGCGTTGGGTCCT 5177

>Vector pET19b_HisDAD2, complete sequence

Sequence ID: MT270501.1 Length: 6431
Range 1: 4710 to 5119

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5119 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 5060

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5059 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 5000

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 4999 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 4940

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 4939 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 4880

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

 |||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 4879 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 4820

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 4819 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 4760

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4759 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 4710

Range 2: 3016 to 3355

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3355 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 3296

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3295 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3236

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3235 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3176

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3175 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3116

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3115 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3056

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3055 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3016

Range 3: 3020 to 3359

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3359 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 3300

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3299 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3240
Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825
||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3239 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3180

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3179 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3120

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3119 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3060

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3059 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3020

Range 4: 4433 to 4693

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4693 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 4634

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4633 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 4574

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4573 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 4514

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 4513 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 4454

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 4453 TCTCGGGCAGCGTTGGGTCCT 4433

>Vector pET19b_DAD2cpGFP(G166)LSSmOrange, complete sequence

Sequence ID: MT270500.1 Length: 7880
Range 1: 5829 to 6238

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6238 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 6179

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

 |||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6178 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 6119

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 6118 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 6059

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 6058 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5999

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5998 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5939

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5938 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5879

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5878 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5829

Range 2: 4135 to 4474

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4474 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 4415

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4414 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 4355

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 4354 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 4295

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 4294 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 4235

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 4234 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 4175

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 4174 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 4135

Range 3: 4139 to 4478

Score:434 bits(480), Expect:3e-116,
Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4478 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 4419

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4418 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 4359

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4358 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 4299

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 4298 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 4239

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 4238 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 4179

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 4178 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 4139

Range 4: 5552 to 5812

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5812 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5753

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5752 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5693

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5692 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5633

Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179

||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5632 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5573

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 5572 TCTCGGGCAGCGTTGGGTCCT 5552
>Vector pET19b_DAD2cpGFP(G166), complete sequence
Sequence ID: MT270499.1 Length: 7136
Range 1: 5415 to 5824

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5824 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 5765

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5764 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 5705

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 5704 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 5645

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 5644 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 5585

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 5584 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 5525

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 5524 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 5465

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5464 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 5415

Range 2: 3721 to 4060

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4060 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 4001

Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4000 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 3941

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 3940 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 3881

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 3880 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 3821
Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260
|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 3820 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 3761

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 3760 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 3721

Range 3: 3725 to 4064

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Minus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4064 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 4005

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 4004 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 3945

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 3944 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 3885

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 3884 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 3825

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 3824 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 3765

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 3764 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 3725

Range 4: 5138 to 5398

Score:350 bits(387), Expect:1e-90,

Identities:231/261(89%), Gaps:30/261(11%), Strand: Plus/Minus

Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023

||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5398 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 5339

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5338 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 5279

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5278 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 5219
Query 1130 CCAACCCTTGG----------CCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 1179
||||||||||| |||||||||||||||||||||||||||||||||||||||
Sbjct 5218 CCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCA 5159

Query 1180 TCTCGGGCAGCGTTGGGTCCT 1200

|||||||||||||||||||||
Sbjct 5158 TCTCGGGCAGCGTTGGGTCCT 5138

>Cloning vector mini-RGC, complete sequence

Sequence ID: MW148404.1 Length: 7974
Range 1: 6836 to 7245

Score:537 bits(595), Expect:4e-147,

Identities:360/410(88%), Gaps:50/410(12%), Strand: Plus/Minus

Query 302 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAG- 360

|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7245 GTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGC 7186

Query 361 ---------ATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 411

|||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 7185 GCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCC 7126

Query 412 TTCGTCACT----------CCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 461

||||||||| |||||||||||||||||||||||||||||||||||||||||
Sbjct 7125 TTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATG 7066

Query 462 GCGGCCGACGCGCTGGGCT----------GGCGTTCGCGACGCGAGGCTGGATGGCCTTC 511

||||||||||||||||||| |||||||||||||||||||||||||||||||
Sbjct 7065 GCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTC 7006

Query 512 CCCATTATGATTCTTCTCGCTTCCGGCG----------GCCCGCGTTGCAGGCCATGCTG 561

|||||||||||||||||||||||||||| ||||||||||||||||||||||
Sbjct 7005 CCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTG 6946

Query 562 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAA----------CGGCTCTTACC 611

||||||||||||||||||||||||||||||||||||||| |||||||||||
Sbjct 6945 TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACC 6886

Query 612 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 661

||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6885 AGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGC 6836

Range 2: 5303 to 5642

Score:438 bits(485), Expect:3e-117,

Identities:297/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 1 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5303 GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC 5362
Query 61 ----------GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 110
||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5363 TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA 5422

Query 111 AGCTCCCTCG----------TGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 160

|||||||||| ||||||||||||||||||||||||||||||||||||||||
Sbjct 5423 AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT 5482

Query 161 CTCCCTTCGGGAAGCGTGGC----------TGCTCACGCTGTACCTATCTCAGTTCGGTG 210

|||||||||||||||||||| |||||||||||| |||||||||||||||
Sbjct 5483 CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG 5542

Query 211 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTG----------CCGTTCAGCCCGACCGCTGC 260

|||||||||||||||||||||||||||||| ||||||||||||||||||||
Sbjct 5543 TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC 5602

Query 261 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 300

||||||||||||||||||||||||||||||||||||||||
Sbjct 5603 GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA 5642

Range 3: 5299 to 5638

Score:434 bits(480), Expect:3e-116,

Identities:296/340(87%), Gaps:40/340(11%), Strand: Plus/Plus

Query 666 GGACGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAA----- 720

|| ||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5299 GGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCG 5358

Query 721 -----CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 775

|||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5359 ACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCC 5418

Query 776 TGGAA----------GCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 825

||||| |||||||||||||||||||||||||||||||||||||||||||||
Sbjct 5419 TGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGC 5478

Query 826 CTTTCTCCCTTCGG----------GCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC 875

|||||||||||||| ||||||||| |||||||||||||||||||||||||
Sbjct 5479 CTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTC 5538

Query 876 GGTGTAGGTCGTTCGCTCCAAGCTG----------ACGAACCCCCCGTTCAGCCCGACCG 925

||||||||||||||||||||||||| |||||||||||||||||||||||||
Sbjct 5539 GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCG 5598

Query 926 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACACG 965

||||||||||||||||||||||||||||||||||||| ||
Sbjct 5599 CTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCG 5638

Range 4: 6630 to 6819

Score:262 bits(290), Expect:1e-64,

Identities:170/190(89%), Gaps:20/190(10%), Strand: Plus/Minus
Query 970 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCG------ 1023
||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6819 AACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGT 6760

Query 1024 ----GTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 1079

||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6759 CGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACG 6700

Query 1080 G----------CCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 1129

| |||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct 6699 GATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAA 6640

Query 1130 CCAACCCTTG 1139

||||||||||
Sbjct 6639 CCAACCCTTG 6630