Syllabus for Ph.D.

In Stem Cell and Tissue Engineering

MM: 50
Paper I: Stem Cell Research Methodology

Note: - Set four questions from each section, for 10 marks each. Candidates should attempt
any five, by selecting at least any two questions from each section.
Section A
Introduction to research methodology, types of research, key steps in research, tools of research,
Introduction to literature collection, its importance and sources, preparing a research report, types
of research reports, and their components, Research designs and hypothesis, basic principles of
research designs, laboratory safety, animal handling.
Section B
Virtual experimental data analysis
Introduction tovirtual experimentation, Experimentation on stem cell transfection, Planning
experiments to demonstrate transcriptional regulation, epigenttic regulation, non genomic
regulation.
Books Recommended /suggested readings
1. Research Methodology, C.R. Kothari, 2009
2. Research Methodology for Biological Sciences, N Gurumani, 2006

Paper-II: Tools, technology and Ethics in Stem Cell and Tissue Engineering MM: 50
Note: - Set four questions from each section, for 10 marks each. Candidates should attempt
any five, by selecting at least any two questions from each section.

Section A
Biotechniques in Stem Cells:

1. Flow cytometery and applications and related techniques

2. Bioinformatics tools in stem cells
3. Mass spectrophotometry and MALDI-TOF,
4. SEM, TEM and Confocal Microscopy
5. Radioisotopes and applications
6. In vitro Fertilization technology and embryo culture
7. Scafolds/ Biomaterials

Section B

Advances in Stem cells and Tissue engineering:

Basic biology of stem cells; Types & sources of stem cell with characteristics: Embryonic, adult,
haematopoetic, fetal, cord blood, placenta, bone marrow, primordial germ cells, cancer stem cells,
induced pleuripotent stem cells.Applications of stem cells, GMP facility, Biomaterials and their
properties and applications, Biologically Active scaffold based on tissue engineering, their
properties and applications, Industrial approach to stem cell & tissue engineering: Introduction,
bioreactors in stem cells and Tissue engineering, Regulations & Ethics: Ethics in use of stem cell,
regulatory bodies for use of engineered biomaterial for human need.
Books Recommended
1. Translational Approaches In Tissue Engineering & Regenrative Medicine” by J. J. Mao, G.
Vunjak-Novakovic etal (Eds), 2008, Artech House, INC Publications.
2. Essential tem cell Methods (2009) Robert Lanza and Irina Klimanskaya (Eds)
3. Hand book of Stem Cells Volume-I & II (2004) Robert Lanza, J Gearhart, B. Hogan,
D. Melton, R. Pedersen, J Thomson and M West (Eds.)
Paper-III:
(A) One Journal Club
(B) One Seminar Club