MTAP SESSION 3: HISTOPATHOLOGY AND MT
10% Formol-saline – best for CNS, general
LAWS
RATIONALE
1. The chemical constituent of the fixative is taken
and becomes part of the tissue in the additive
mechanism of fixation. Which of the fixatives
listed below does not have this property?
a. 10% NBF
b. Zenker’s Fluid
c. Carnoy’s Fluid
d. Flemming’s Fluid
Carnoy’s fluid is non-additive since it is
under alcoholic fixative
2. Which of the ff fixatives can be used as a nuclear
and histochemical fixative at the same time?
a. Helly’s – Cytoplasmic
b. Bouin’s - Nuclear
c. Newcomer’s
d. Heidenhain’s susa - Nuclear
Cytoplasmic: HORFF; Nuclear: BFNCH;
Histochemical: 10F-ANA
3. Skin tumor biopsies are preserved with which of
the following fixatives?
a. B-5 fixative – Bone marrow
b. Heidenhain’s Susa – “only Belo touches my
skin”
c. Bouin’s – embryos and pituitary biopsies and
endometrial curettings, fragmentary biopsies
– it stains tissue, yellow; under picric acid
d. Zenker-Formol – aka Helly’s; “BLOODY
HELLY” ; for blood-containing organs such as
liver, spleen, bone marrow, and pituitary
gland
4. The adhesive agents used for cytology include all
of the following, except:
a. Leuconostoc culture
b. Egg albumin
c. Celloidin ether alcohol
d. Serum or plasma
Egg albumin is not recommended because it
is intensely stained by Basic light green
counterstain of Pap method
5. Since most formalin fixatives are inert to lipids,
this one is used for lipid fixation:
a. 10% NBF – best general tissue fixative (also
Zenker’s); for frozen sections, iron-containing
pigments, and elastic fibers
b.
post-mortem tissues for histochemical exam,
silver impregnation
c. Formol calcium
d. Alcoholic formalin aka Gendre’s fixative-
recommended for sputum cytology
6. It’s possible that using this formaldehyde fixative
will result in the formation of black precipitates
a. Formol corrosive – mercuric deposits;
routine post-mortem; silver reticulum
b. 10% formol-saline
c. Calcium acetate formalin aka Lillie’s fixative
– preserves phospholipids
d. Alcoholic formalin – sputum cytology
7. In routine H & E staining method for paraffin
embedded tissues, plasma cells and osteoblasts
will be stained what color?
a. Deep pink – for muscle fiber
b. Dark blue – for karyosome
c. Purplish blue – for CC (Calcium and calcified
bone)
d. Purplish pink
See table in the reviewer
8. Orth’s fluid is an excellent fixative for identifying
which of the following organisms?
a. Rickettsia
b. Fungi
c. Viruses
d. Protozoans
Orth’s fluid is recommended for early
degenerative processes and tissue necrosis
9. The standard fixative for preserving cell detail in
tissue photography is:
a. Glutaraldehyde – histochemistry and EM
b. Zenker’s fluid – mercuric chloride
c. Formol calcium – lipids preservation;
specialized fixative used in frozen section
d. Gendre’s – sputum cytology
10. In 1848, this person invented the freezing
microtome
a. Queckett – “Quick = freezing”
b. Paldwell treefall – Rocking/Cambridge
Microtome (1881)
c. Adams – Sliding Microtome (1789)
d. Minot – Rotary microtome (1885-1886)
11. If you leave the tissue in a watery solution, the
cell will:
a. Dry out
b. Swell
 c. Shrink – hypertonic
d. All of these
Watery = hypotonic
12. The most popular and widely used microtome in
both routine and research laboratories; it was
developed by: ____
a. Rocking; Minot
b. Rocking; Paldwell Trefall
c. Rotary; Minot
d. Rotary; Paldwell Trefall
Rotary : paraffin sections ; Rocking : large
blocks of paraffin-embedded tissue
13. Microtome knives are designed based on the
tissue to be sections as well as the embedding
medium. Which of the following statements
about microtome knives is correct?
1. Plane concave – the concave side is used to cut
paraffin sections
2. Plane wedge – for cutting frozen sections and
extremely hard and tough specimens
3. Biconcave – for cutting celloidin sections
a. 3 only
b. 1 and 2
c. 2 and 3
d. 1, 2, and 3
Biconcave are used for cutting paraffin
sections
14. An excellent microanatomic fixative for the
pituitary gland, bone marrow, and blood-
containing organs
a. Zenker’s – good general tissue fixative (like
10% NBF [best])
b. Helly’s or the Zenker-formol
c. Heidenhain’s Susa – tumor biopsies
especially of the skin
d. Schaudinn’s – aka Sublimated alcohol (used
on wet smears for cytologic examination)
15. The fixative recommended for demonstrating
mitochondria, chromatin, golgi bodies, mitotic
figures, RBCs, & colloid-containing tissues.
a. Regaud’s aka Moller’s fluid
b. Potassium dichromate – preserve
mitochondria; fixes lipids
c. Chromic Acid – ppts proteins & preserves
CHO
d. Orth’s – Rickettsia & other bacteria
demonstration; early degenerative processes
& tissue necrosis
These are all under chromates – “CROP”
16. The following are Mercuric chloride fixatives:
1. Carnoy Lebrun
2. Heidenhain’s susa
3. Orth’s - chromate
4. Ohlmacher
5. Schaudinn’s
a. 1, 2, 3, and 4
b. 1, 2, 3 and 5
c. 1, 2, 3, 4 and 5
d. 1, 2, 4, and 5
Zenker’s, Zenker-Formol (Helly’s), and B-5 (bone
marrow) are also under Mercuric chloride.
Heidenhain’s susa does not form black ppt.
17. Which of the following is the correct order of the
steps in tissue processing?
1-Decalcification 2-Fixation 3-Impregnation
4- Clearing 5-Dehydration
a. 2,5,1,4,3
b. 2,1,5,3,4
c. 2,1,5,4,3
d. NOTA
18. Which of the following fixatives is explosive when
dry?
a. Bouin’s – Picric acid is explosive when dry
b. Lead – takes up CO2 to form insoluble lead
carbonate; for acid mucopolysaccharides
c. Zenker’s – formation of black ppts; contains
glacial acetic acid which makes the solution
unstable
d. Flemming’s – produce black ppts and yellow
color which depresses the staining power of
Ehrlich’s hematoxylin; prolonged exposure to
vapor can cause conjunctivitis or blindness.
Picric acid must be kept moist with distilled
wtater or 0.5-1% saturated alcohol
19. Which of the following factors hastens fixation?
a. Large and thick tissues
b. Presence of fats, mucus, and blood
c. Heat and agitation (moderate heat” 37-56ºC)
d. Hot or cold temperature
20. Which of the following is used as a section
adhesive in immunohistochemistry?
a. Poly-L-Lysine – second best
b. APES – for cytology (cytopsin prep of
proteinaceous and bloody materials) - best
c. Mayer’s egg albumin – most commonly used
(easy to make and relatively inexpensive)
d. Starch paste
 Dried albumin, 1% gelatin, Gelatin-
formaldehyde mixture, plasma
21. Which of the following could be the cause of soft
and feathery tisues?
a. Prolonged fixation – too brittle/hard blocks
b. Incomplete fixation
c. Wrong choice of fixative – removal of
fixative soluble substances; enzyme
inactivation and loss
d. Overfixation – shrinkage and swelling
Presence of artifact pigments is due to
incomplete washing. If there are parts of
tissues that undergone autolysis, this maybe
due to failure of fixing immediately or
insufficient fixative volume (20x the volume
of the tissue)
22. Which of the fixatives listed below is classified as
histochemical?
1. Orth’s -cytoplasmic
2. Newcomer’s
3. Helly’s - cytoplasmic
4. Acetone
a. 1,2, and 3
b. 1 and 3
c. 2 and 4
d. 1,2,3, and 4
23. The fixative that is recommended for nuclear
structures such as chromosomes is:
a. Bouin’s – embryos, pituitary biopsies,
endometrial curettings
b. Brasil’s – best routine for glycogen
c. Fleming’s
d. Flemming’s without acetic acid –
cytoplasmic structures
24. The steps involved in automatic tissue processing
(Autotechnicon/Eliot-bench type tissue
processor)
a. Fixation, Decalcification, Clearing,
Impregnation
b. Fixation, Dehydration, Clearing, Embedding
c. Fixation, Dehydration, Clearing, Infiltration
d. Fixation, Decalcification, Clearing,
IInfiltration
25. It has the ability to act both as a fixative and a
decalcifying agent:
a. Prenyi’s fluid – weak decal agent and tissue
softener
b. Trichloroacetic acid
c. Alcohol – fixative and dehydrating agent
d. Tetrahydrofuran – dehydrating and clearing
agent
26. If the specimen was fixed in Zenker’s fluid, the
extent of decalcification could not be determined
by:
a. Calcium oxalate test – routine; chemical
b. Mechanical method – touching/bending
c. X-ray method or Radiological Method; most
ideal but not convenient and expensive
d. None of the Above
Metal content – mercury - can cause radio
opacity that could interfere with plate
interpretation
Cloudiness = incomplete decalcification
27. Which of the following fixatives is used in the
preservation of brain tissues for rabies diagnosis?
1. Acetone – brain tissue for rabies diagnosis
(Negri bodies); free substitution
2. Newcomer’s – mucopolysaccharides and
nucleoproteins
3. Trichloroacetic acid
a. 1 and 2
b. 1 and 3
c. 1 only
d. None of these
Carnoy’s fluid can also be used; it is also the
most rapid
28. What is the primary aim of fixation?
a. To facilitate easy cutting
b. To harden and protect the tissue
c. To stabilize the protein through cross-link
formation
d. To maintain the physical and chemical
characteristics of the tissue
29. Which of the following statements is erroneous?
a. Tap water us used to remove excess amount
of osmic acid in tissues
b. 50-70% Ethanol is used to remove excess
amount of Picric acid in t issues
c. Alcoholic iodine Is used to remove excess
amount of chromate in tissues
d. Tap water is used to remove excess amount
of formalin in tissues
Alcoholic iodine is for excess mercuric
chloride. Tap water is for excess chromate,
formalin, and osmic acid
30. Which fixative should be used to demonstrate
acid mucopolysaccharides?
 a. Picric acid – for glycogen demonstration;
yellow fragmentary biopsy
b. Mercuric chloride – cell detail in tissue
photography; permits brilliant
metachromatic staining
c. Lead
d. Chromate – “CAM”
31. Which basic dye should be used to demonstrate
th granule and vacuoles of phagocytic cells
a. Congo Red – for axis cylinders in embryos;
indicator
b. Acridine Red 3B – calcium slats and
phosphatase activity
c. Janus Green B - mitochondria
d. Neutral Red
32. Recommended for the study of water diffusible
enzymes especially lipases and phosphatases
a. Glacial acetic acid – fixes and ppts
nucleoproteins, chromosomes, and
chromatin materials
b. Trichoroacetic acid – weak decal agent and
suitable for small pieces of txs or bone
because of poor penetration
c. Acetone
d. Heat fixation – rapid diagnosis employed for
frozen section and prep of bacteriologic
smears
33. What temperature is typically used for fixation in
Electron microscopy?
a. Refrigerator temperature – 0-4ºC
b. Freezing temperature
c. Incubation at 37ºC
d. Room temperature – routine (18-30ºC)
For autotechnicon: 40-42ºC
34. Dehydration is accomplished through the use of:
a. Increasing grades of alcohol – begin with
70%, 95%, and 100%
b. Increasing grades of xylene
c. Decreasing grades of alcohol
d. Decreasing grades of xylene
35. Which of the following is the primary
disadvantage of alcoholic fixatives?
a. RBC lysis
b. Dissolves fats and lipids
c. Tissue shrinks on long usage
d. The streaming of the glycogen granules to
one pole of the cells
All are correct but d is the best answer
Alcoholic Fixatives= “CEMING”
36. The temperature of the water bath in tissue
processing is kept at
a. 2-5ºC above the melting point of the wax
b. 2-5ºC below the melting point of the wax
c. 6-10ºC above the melting point of the wax
d. 6-10ºC below the melting point of the wax
Infiltration = paraffin oven = 2-5ºC above the
melting point of the wax
Embedding = 5-10ºC above the melting point
of the wax
37. Which of the following stains is used to
demonstrate the cytoplasmic structure of
intermediate and parabasal cells in Pap staining
technique
a. Hematoxylin – nuclear stain
b. Orange Green 6 – cytoplasmic structures of
mature cells
c. Eosin Azure – cytoplasmic structures of
immature cells such as intermediate and
parabasal cells
d. NOTA
38. Which of the following decalcifying agents can
also be used to soften tissue?
a. Perenyi’s fluid
b. Sulfurous acid – weakest decal agent
c. Phloroglucin nitric acid – most rapid decal
agent; recommended for urgent works
d. Hydrochloric acid – surface decalcification
Von Ebner’s fluid – 36% saturated aq. NaCl +
Conc’t HCl + Distilled water
39. Fixative for nervous tissue that is also suitable for
Silver Impregnation
a. 10% NBF
b. 10% Formol-Saline
c. Formol corrosive – for silver reticulum
d. Gendre’s
40. Sputum cytology requires which of the following
fixatives
a. Calcium acetate formalin - phospholipids
b. Formol sublimate
c. Alcoholic formalin
d. 10% NBF
41. Which of the following is not a nuclear fixative?
a. Bouin’s
b. Regaud’s – cytoplasmic
c. Heidenhain’s susa
d. Carnoy’s
42. Which of the following adhesives is very useful in b. 10-25x the volume of the tissue to be fized
cytology particularly fopr cytospin preparations c. 15-20x the volume of the tissue to be fixed
of proteinaceous or nloody materials d. 20x the volume of the tissue to be fixed
a. Poly-L-Lysine This is because it is very expensive
b. APES 48. Which of the following is incorrectly paired?
c. Mayer’s egg albumin a. Vaginal scrape – endocervical canal
d. Starch paste b. Vulvar scrape – herpetic lesion or carcinoma
43. The following are aqueous mounting media, c. Lateral vaginal scrape – hormonal evaluation
except: d. None of these
1. Clarite Vagina scrape – for pxs with hysteroctomy
2. Apathy’s medium Endocervical brush – endocervical canal
3. Brun’s fluid Localization of vaginal adenosis – four-
4. Xam quadrant vaginal scrape
5. Glycerin T-zone is important
a. 1,2, and 4 49. Used as an aqueous mountant for Methylene
b. 1,3, and 4 Blue-stained nerve preparations as well as a
c. 1, and 4 general purpose aqueous mountant
d. 4 only a. Water – good only for temporary moounting
Aq med: Water, glycerin, farrant’s (Gum b. Glycerin – high index of refraction;
syrup), Apathy’s, Brun’s preservative
Clarite, Xam, DPX, and Canada balsam are c. Apathy’s
resinous mounting media d. Brun’s – recommended for mounting frozen
44. Polyclonal antibodies are produced after an sections from water or paraffin sections
animal is immunized with an immunogen Canada balsam - ____
containing the antigen of interest. In this context, 50. The following statements are true, except:
what is the mos commonly used animal? a. Methyl salicylate is used when double
a. Mouse embedding techniques are required
b. Horse b. Cedarwood oil is recommended for CNS
c. Sheep tissues and cytological studies
d. Rabbit c. Chloroform does not make tissues
For monoclonal = hybridoma/cloning transparent and it is toxic to the liver on
technique = mice prolonged inhalataion
45. Which of the following is the most ideal, d. Clove oil is used for clearing embryos,
sensitive, and reliable method for determining insects, and very delicate specimens
the extent of decalcification? Aniline oil is the correct term
a. Physical method Clove oil has the tendency to be adulterated,
b. Radiological method thus quality is not guaranteed
c. Chemical method – most favored 51. In cervical cytology, normal cells with pale blue
d. Mechanical method or gray finely vacuolated cytoplasm are
46. What temperature does formalin have to be a. Endometrial cells
heated for rapid fixation of very urgent biopsy b. Endocervical glandular cells – honeycomb
specimens? appearance
a. 100ºC - to fix tissues with TB c. Pregnancy cells – oval, nucleus is eccentric,
b. 80ºC double-walled boundary
c. 60ºC d. Intermediate cells – medium-sized,
d. 50ºC basophilic cytoplasm, with vacuoles,
47. When using osmium tetroxide solution in elongated/polyhedral, boat-like = navicular
fixation, the recommended volume is: cells
a. 5-10x the volume of the tissue to be fixed
52. Excessive exposure to this clearinga gent can be a. 1,2,3,4
extremely toxic and carcinogenic, or it can b. 1 and 4
damage the bone marrow and cause aplastic c. 2 and 3
anemia d. 1 and 3
a. Chloroform – hepatotoxic and does not Karnovsky: Parafolrmaldehyde-
make the tissue transparent glutaraldehyde mixture
b. Tetrahydrofuran – eye and skin irritant Acrolein: glutaraldehyde and formaldehyde
c. Benzene 59. The thickness of a paraffin section is
d. Carbon tetrachloride – same with a. 10-15 µ ˆ- for celloidin
Chloroform but more toxic and cheaper b. 4-6 µ
53. Double embedding methos id the process in c. 2-4 µ
which tissues are first infiltrated with _____ and d. 1-2 µ
subsequently embedded in _____ mass. 4µ for frozen section and 0.5µ for ultrathin
a. Celloidin; Paraffin (EM)
b. Gelatin; Paraffin 60. Which of the following types of hones is said to b
c. Celloidin; Gelatin the finest?
d. Paraffin; Celloidin a. Yellow Belgian
54. Substitute for xylene or benzene as a clearinga b. Carborondrum – wide variety of fineness;
gent used only for badly nicked knives
a. Tetrahydrofuran – clears and dehydrates at c. Arkansas stone – medium fineness
the same time d. Belgian Black Vein – aka Oil stones
b. Chloroform 61. Which of the following stains is used to
c. Toluene demonstrrate mitochondria?
d. Clove oil a. Van Gieson’s aka Acid fuchin picric acid for
55. The best fixative for cytological specimen connective tissues, mucin& elastic fibers
requiring fixation b. Congo red – axis cylinders in embryos
a. 95% Ethanol – most commonly used c. Trypan Blue – vital stain of RES
b. Pap’s fixative d. Jnaus Geen B
c. Saccomanno’s Fixative : 50% ethanol + 2% 62. A sample from a blood unit was reactive in ELISA
Carbowax – for Fine-needle aspiration, urine, (anti-HIV ½). A similar result was obtained after
sputum, pleural fludis repeat testing with the same sample. To which of
d. Ether-Alchol the following reference laboratories will you
Ether is flammable, volatile, and has fire send out the sample for confirmatory testing?
hazards a. RITM
56. The following are natural dyes, except: b. SACCL
a. Aniline – synthetic dyes; coal tar; derive from c. NKTI
hydrocarbon Benzene d. EAMC
b. Hematoxylin 63. The Father of PAMET
c. Orcein a. Hector Gayares, Jr. – lyrics of PAMET hymn
d. Saffron (Beloved PAMET)
Hematoxylin, Orcein, Cochineal/Carmine, b. Francis Pefanco – melody and music of
and Saffron are the natural dyes PAMET hymn
57. Composition of Kronig cement: c. Charlemagne Tamondong – emergence of
a. 4 to 9 parts of ______ the profession; first PAMET President (BS
58. Which of the fixatives listed below is used in EM? Hygiene graduate)
1. Glutaraldehyde d. Crisanto Almario
2. 10% NBF 64. This class of fixative cause excessive yellow
3. Zenker’s fluid stainig of tissues
4. Osmium Tetroxide a. Picrates
 b. Chromates – strong oxiding agent used for
ppting proteins and preserving CHO; dor
COM
c. Aldehydes – acrolein is the simplest
aldehyde
d. Alcohols
65. Paraformaldehyde deposits are formed when
formalin is stored a long time in _____
temperature, but this can be remedied by using
_____.
a. Warm; acid alcohol
b. Cold; acid alcohol
c. Cold; methanol
d. Warm; methanol
66. PAMET hymn music by:
a. Hector Gayares, Jr.
b. Francis Pefanco
c. Charlemagne Tamondong
d. Crisanto Almario
67. The ideal size of tissue to be fixed:
a. It must be at least 2mm thick
b. Not more than 2 mm in thickness
c. It must be at least 4 mm thick
d. Not thicker than 4mm
68. Suitable for specimens containing large cavities
or hollow spaces which tend to collapse, for hard
and dense tissues and for large sections of the
whole embryos
a. Paraffin wax impregnation
b. Celloidin Wax impregnation
c. Gelatin impregnation – rarely used except
when dehydratin is to b avoided;l
histochemical and enzyme studies
d. Resin/Plastic Impregnation
69. The temperature utilized in autotechnicon
a. 0-4
b. 18-30
c. 40-42
d. 60-100
70. Why does Xylene turn milky when tissue is
transferred to it?
a. Clearing agent not hot enough
b. Inadequate fixation
c. Prolonged storage – cedarwood oil
d. Insufficient dehydration
71. Senate Bill 2722 is also referred to as
a. Medical Technology Act of 2004
b. Medical Technology Act of 2011
c. Medical Technology Act of 1969
d. Medical Technology Act of 2018 – SB No.
1891
72. Cryostat chamber is set at what temperature?
a. 1-10ºC
b. -1 to -10ºC
c. -18 to -20ºC
d. -27 to -32ºC
73. Which of the following impregnation methods is
used for delicate specimens and frozen tissue
sections because it prevents fragmentation and
friable tissue
a. Gelatin impregnation
74. Which of the following embedding media is
recommended for EM?
a. Gelatin
b. Epoxy
c. Celloidin
d. Paraffinm
75. It is also known as the New Born Screening Act of
2004
a. RA 9288
b. RA 6969 – Toxic and hazardous waste
c. RA 8504 – HIV/
d. RA 9165 – Comprehensive Dangerous
76. Refractive Index of Glass slide
a. 1.516
b. 1.518
c. 1.520
d. 1.480
77. Sliding microtome is used for cutting celloidin
embedded sections; rotary microtome is used for
cutting paraffin embedded section
a. Both statements are correct
b. Both statements are incorrect
c. First statement is correct; second statement
is incorrect
d. First statement is incorrect; second
statement is correct
78. The following statement are erroneous except:
a. Honing is the process whereby the burr is
removed and the cutting edge of knife is
polished
b. Stropping is the removal of gross nicks and
irregularities on the knife edge
c. The direction of the honing process in heel-
to-toe
d. None of these
79. The selective staining of living cell constituents,
demonstrating cytoplasmic structures by
 phagocytosis of the dye particle (Cytoplasmic
phagocytosis)
a. Vital staiinig
b. Supravital staining – stainig immediately
after removal from the living body
c. Intravital staining - injecting
80. Paraffin sections – for paraffin embedded tissue
blocks and may be cut by rocing and sliding
microtome (this should be rockinga ng rotary)
Celloiding sections – for celloidin embedded
tissues and usually cut by means of the rotary
microtome (this should be sliding)
a. Both statementas are true
b. Both statement are false
c. First statement is true, second statement is
false
d. First statement is false, second statement is
true
81. The stain is used for staining neuroglia in frozen
sections
a. Night Blue – subctitude for Carbolfuchsin in
AF staining
b. Alcian Blue – for acid mucopolysaccharides
c. Victoria Blue
d. Celestine Blue - rouytine staiing of fixed
tissues
82. Which of the following is the authorized bodfy
for matters concerning nuclear/radioactive waste
in the Philippines?
a. DOH
b. PNRI and DENR (RA 6969)
c. Philippine Environmental Management
Bureau
d. Mother Earth Foundation
83. Which of the following fixative -other name
pairings is incorrect
a. Formol sublimate – Formol corrosive
b. Alcoholic formalin – Gendre
c. Zenker-formol – Moller
d. Osmic Acid- Osmium tetroxide
Zenker formol – Helly’s; Moller - Regaud
84. Which of the following fixatives is recommended
for fixing endometrial curetting?
a. Methanol dry and wet smears,
b. Bouin’s
c. B-5 – BM biopsy
d. Flemming’s – for nuclear structures such as
chromosomes
85. The following are commonly used dyes for
intravital staining
1. Carmine
2. Eosin
3. Lithium
4. India Ink
a. 1, 3, amd 4
86. The most rapid fixative is from what general
fixative group?
a. Formalin
b. Alcohol
c. Metallic Group
d. Picric Acid
87. Freezing microtome have been replaced in most
laboratories by the Cryostat, which is easier to
operate, faster, and gives thicker section (it
should be thinner). Frozen sections may be cut
from fixed or fresh tissue, but generally fixed
enzyme preparation
a. First statement is flase, second statement is
true
88. What section of RA 5527 specifies the functions
and duties of the Board of Examiners
a. 20
b. 9- Executie Officer of the board
c. 11
d. 27 – Foreign reciprocity
89. Which of the following statement is correct?
a. Rotary microtome is also known as
Cambridge microtome
b. Rocking microtome is the microtome inside
the cryostats
c. Rotary microtome is the simplest microtome
d. Sliding microtome is the most dangerous tye
of microtome
90. Which of the following statements are correct?
a. The volume of 10% NBF should be 20x the
volume of the tissue
b. The volume of the ethanol should be 10
times the volume of the tissue
c. The colume of Xylene should be 10x the
volume of the tissue
d. The volume of Nitric acid should be 20x the
volume of the tissue
All are correct
91. The most commonly used impregnation agent,
and it is used to demonstrate nervous tissues and
reticulin
a. Silver nitrate
 b. Osmium tetroxide – fixative and stain for c. RA 4688: Clinical Lab Law
fats 98. The most rapid impregnation technique is
c. Cochineal – natural dye ______; it is impregnation under _____
d. Neutral Red – cell granule sand vacuoles of - Vacuum; negative pressure
phagocytic cells 99. Commonly used dehydrating agents:
92. The third amendment to the medical technology - Alcohol, acetone, and tetrahydrofuran
act of 1969: - Cedarwood is clearing
a. RA 6138 – 1st amendment (Sec. 16,21,22) 100. If administrative investigatrion… majority
b. PD 1534 vote.
c. PD 498 – 2nd amendment (Sec a. Suspension of COR for 1 year
2,3,4,7,8,11,13,16,17, 21,29) b. Suspension of COR for 2 years
d. RA 4688 – Clin Lab Act of 1966 c. Suspension of COR for 4 years
93. A chemical substance that does not participiate d. Revocation of COR
but merely increases or heightens the color
intensity, crispness, and sledctivity of the stain
a. Mordant – acts as link or bridge
b. Accentuator
c. Lake – mordant + dye
d. Differentiator – selective removal of excess
dye
94. The presence of airholes in the tissue during
trimming is caused by:
a. Incomplete fixation
b. Prolonged clearing
c. Incomplete impregnation
d. Drying out of tissue before actual fixation
95. The following statemnts about formalin fixatives
are coorect, except
a. Lillie’s fixative is buffered to pH 7 by
carbonate (it should be by acetate)
b. 10% NBF is the best general tissue fixative
c. 10% FS is the best fixative for nervous tissue
d. Gendre’s fixative contains ethanol saturated
with picric acid
96. Which of the following pairs are correct?
a. Hematoxylin – the most important and from
Mexican heartwood
b. Orcein – a vegetable dye extracted from
lichens which are normally colored
(colorless)
c. Carmine – a dye derived from an extract
from the female Cochineal Bug (Coccus cacti)
d. Saffron – a plant with orange stigmas
yielding die
A,C, and D are correct
97. Which if the following is incorrectly matched?
a. RA 9165: An act to control toxic substances
and hazardous nuclear wastes (RA 6969)
b. RA 8981: Modernizing PRC