Antibacterial Finishes on Textile Materials: Assessment of
Developed in 1961 by AATCC Committee details such as material safety data sheets can Type Culture Collection No. 4352. RA31; revised 1965, 1981, 1988 (with ti- and other manufacturer’s recommenda- Gram negative organism (see 13.10). tle change), 1993, 1999; editorially re- tions. All OSHA standards and rules 6.1.3 Other suitable species can also be vised 1969, 1971, 1974, 1985; must also be consulted and followed. used. reaffirmed 1977, 1981, 1989, 1998; edi- 4.1 Both the qualitative and quantita- torially revised and reaffirmed 1986, tive tests should be carried out by persons 7. Culture Medium 2004. with training and experience in the use of 7.1 Suitable broth/agar media are Nu- bacteriological techniques. The U.S. De- trient, Trypticase Soy and Brain-Heart partment of Health and Human Services 1. Purpose and Scope Infusion. publication, Biosafety in Microbiological Nutrient Broth: 1.1 This test method provides a quanti- and Biomedical Laboratories, should be Peptone (Bacto-peptone) tative procedure for the evaluation of the consulted (see 13.1). (see 13.3) 5g degree of antibacterial activity. Assess- 4.2 CAUTION: Some of the bacteria Beef extract (see 13.4) 3g ment of antibacterial finishes on textile used in this test are capable of infecting Distilled water to 1000 mL materials is determined by the degree of humans and producing disease. There- 7.2 Heat to a boil to disperse ingre- antibacterial activity intended in the use fore, every necessary and reasonable pre- dients. Adjust to pH 6.8 ± 0.1 with 1N of such materials. If only bacteriostatic caution must be taken to eliminate this sodium hydroxide (NaOH) solution. (This activity (inhibition of multiplication) is risk to the laboratory personnel and to is not necessary if prepared, dehydrated intended, a qualitative procedure which personnel in the associated environment. medium is used.) clearly demonstrates antibacterial activity Wear protective clothing and respiratory 7.3 Dispense in 10 mL amounts in con- as contrasted with lack of such activity by protection that prevents penetration by ventional bacteriological culture tubes an untreated specimen may be accept- the bacteria. (i.e., 125 × 17 mm). Plug and sterilize at able. However, if bactericidal activity is 4.3 Good laboratory practices should 103 kPa (15 psi) for 15 min. intended or implied, quantitative evalua- be followed. Wear safety glasses in all 7.4 Nutrient agar. Add 1.5% bacterio- tion is necessary. Quantitative evaluation laboratory areas. logical agar to nutrient (or appropriate) also provides a clearer picture for possi- 4.4 All chemicals should be handled broth (see 7.1). Heat to boiling. Check ble uses of such treated textile materials. with care. pH and adjust to 7.1 ± 0.1 using NaOH 4.5 An eyewash/safety shower should solution if necessary. Dispense in 15 ± 1 2. Principle be located nearby for emergency use. mL amounts in conventional bacteriolog- 2.1 Swatches of test and control textile 4.6 Sterilize all contaminated samples ical culture tubes. Plug and sterilize at and test materials prior to disposal. 103 kPa (15 psi) for 15 min. (May be materials are tested qualitatively for anti- bacterial activity by AATCC Method 147. 4.7 Exposure to chemicals used in this sterilized in 1000 mL borosilicate glass Those showing activity are evaluated procedure must be controlled at or below flasks and petri dishes poured from this.) quantitatively. Test and control swatches levels set by government authorities (e.g., 7.5 Slurry Inoculum Carrier (for hy- are inoculated with the test organisms. Occupational Safety and Health Adminis- drophobic fabrics) (see 7.2 and 7.3): After incubation, the bacteria are eluted tration’s [OSHA] permissible exposure Sodium Chloride 8.5 g from the swatches by shaking in known limits [PEL] as found in 29 CFR Agar 3.0 g amounts of neutralizing solution. The 1910.1000 of January 1, 1989). In addition, Distilled Water 1000 mL number of bacteria present in this liquid is the American Conference of Governmen- determined, and the percentage reduction tal Industrial Hygienists (ACGIH) Thresh- 8. Maintenance of Culture of Test by the treated specimen is calculated. old Limit Values (TLVs) comprised of time Organisms weighted averages (TLV-TWA), short term exposure limits (TLV-STEL) and ceiling 8.1 Using a 4 mm inoculating loop, 3. Terminology limits (TLV-C) are recommended as a gen- transfer the culture daily in nutrient (or 3.1 activity, n.—of an antibacterial eral guide for air contaminant exposure appropriate medium) broth for not more agent, a measure of effectiveness of the which should be met (see 13.2). than two weeks. At the conclusion of two agent. weeks, make a fresh transplant from stock 3.2 antibacterial agent, n.—in tex- 5. Limitations culture. Incubate cultures at 37 ± 2°C (99 tiles, any chemical which kills bacteria ± 3°F) or other optimal temperature. (bactericide) or interferes with the multi- 5.1 For a qualitative, relatively quick 8.2 Maintain stock cultures on nutrient plication, growth or activity of bacteria and easily executed method to determine or appropriate agar slants. Store at 5 ± (bacteriostat). residual antibacterial activity of textile 1°C (41 ± 2°F) and transfer once a month materials, refer to AATCC Method 147, to fresh agar (see 13.5). 4. Safety Precautions Antibacterial Activity Assessment of Textile Materials: Parallel Streak Method. 9. Qualitative Test (Screening or NOTE: These safety precautions are Presumptive Test) for information purposes only. The pre- 6. Test Organisms cautions are ancillary to the testing proce- 9.1 For detection of bacteriostatic ac- dures and are not intended to be all inclu- 6.1 Test bacteria. tivity use AATCC Method 147 on a test sive. It is the user’s responsibility to use 6.1.1 Staphylococcus aureus, American specimen and control specimen using the safe and proper techniques in handling Type Culture Collection No. 6538. Gram organisms referred to above. For demon- materials in this test method. Manufac- positive organism (see13.10). stration of bactericidal activity, proceed turers MUST be consulted for specific 6.1.2 Klebsiella pneumoniae, Ameri- to the quantitative test described below.
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