EXperiment 10

Test for pathological

urine and analysis for
urine creatinine
 GROUP 10
VILLAR, ALLEN JAY B.
VERA, DEXTER GABRIEL T.
TRONCO, FEBE SHAINE O.
TAN, KIANNA NICOLE G.
TANG, JOHN ARWEN L.
LEARNING OUTCOMES
Students will understand the principles of
pathological urine testing, including the
identification of pathological constituents.

Students will demonstrate the ability to

measure and interpret the significance of
urine creatine levels accurately.
 introduction
The evaluation of pathological urine is a
comprehensive analysis, essential to diagnose
potential health issues. A pivotal component of
this diagnostic process is the assessment of
urine creatinine levels, a key indicator of renal
function and overall metabolic balance.
 BASIC CONCEPTS
PATHOLOGICAL URINE TESTING
DEFINITION PURPOSE
A systematic examination of urine to detect and Identifies abnormalities in urine that can signal the
diagnose various medical conditions. It includes presence of diseases such as urinary tract
the assessment of physical, chemical, and infections, kidney disorders, or metabolic
microscopic properties of urine. conditions.

URINE CREATININE ANALYSIS

DEFINITION PURPOSE
A diagnostic test that measures the concentration Evaluates overall metabolic status and identifying
of creatinine, a waste product from muscle metabolic disorders. A key tool to assess renal
metabolism in urine. function, as deviations from normal levels.
RESULTS
TESTS FOR PATHOLOGICAL
CONSTITUENTS
A. ROTHERA’S NITROPRUSSIDE TEST
KETONE BODIES:

A chemical test used to detect the presence of

ketone bodies, specifically acetoacetate, in urine.
It reacts with nitroprusside reagent under alkaline
conditions, producing a purple coloration.

B. BENEDICT’S TEST
GLUCOSE:

A chemical test employed to detect the presence

of reducing sugars, primarily glucose, in a
solution. A color change from blue to green,
yellow, orange, or brick red, depending on the
amount of sugar present.
 C. ALBUMIN:
HELLER’S RING TEST
A chemical test used to detect the
presence of albumin in urine, a white ring
formation at the junction of the acid and
urine layers signifies the presence of
albumin.

D. BILE AND BILE PIGMENTS:

GMELIN’S TEST
A chemical test used to detect the
presence of bile pigments, leading to the
formation of a series of colors ranging
from green to blue, depending on the
concentration of bilirubin.
 BILE ACID AND SALTS:
E. PETTENKOFER’S TEST
A chemical test used for the detection of
bile salts. The addition of sulfuric acid and
concentrated furfural creates a red to violet
coloration, indicating the presence of bile
salts.
 ANALYSIS
PATHOLICAL RESULTS AND
CONSTITUENTS TESTS OBSEVATIONS CONCLUSION
Rothera’s Nitroprusside purple coloration is indicates the presence
Ketone Bodies Test visible of acetoacetic acid

Benedict’s brick-red indicates the presence

Glucose Test coloration is visible of reducing sugars
Heller’s Ring formation of white indicates the presence
Albumin Test precipitate of proteins

Bile and Bile Gmelin’s green to blue indicates the presence

Pigments Test coloration is visible of biliburin

Bile and Acid Pettenkofer’s red coloration is indicates the presence

Salts Test visible of bile
RESULTS
QUANTITATIVE ANALYSIS
OF URINARY CREATININE
 ANALYSIS ABSORBANCE 520 NM
SOLUTION CONCENTRATION REPLICATE REPLICATE
1 2
Standard Sol’n 1 0.4 0.171 0.259
Standard Sol’n 2 1.2 0.473 0.495
Standard Sol’n 3 2 0.764 0.724
Standard Sol’n 4 UNKNOWN 1.421 1.075
Blank 0 0.000 0.000
CALCULATIONS
THANK YOU!