distanc per

e (cm) minute
50
NAME: ____________________________________________ SCORE: _________ 100
SECTION: __________________________________________ DATE: __________

LABORATORY NO. 4
ELODEA
Part I. 9 Light color: ________ Graph: ____________
Pre-Lab: Importance of Light in Photosynthesis Light Bubbles
distanc per
Bubbles are given off by the plant through photosynthesis. By measuring e (cm) minute
the rate at which the bubbles are produced it is possible to tell how fast the plant is
50
photosynthesizing.
100
VIRTUAL LAB: Go to: https://amrita.olabs.edu.in/?sub=79&brch=16&sim=126&cnt=4

Next, you will be conducting an experiment using the simulation from the website
to determine which light spectrum greatly affects the rate of photosynthesis.
Assume that all conditions (e.g. soil, moisture and plant, etc..) other than color are
the same.

Procedure:
1. Select the power source.
2. Select the distance of the power source.
3. Select the color of the light/filter.
4. Then, press start.
5. Record the bubbles per minute for each of the following light distances.
Then graph your data. Be sure to label the axes, give units, use
appropriate intervals, and give a descriptive title.
Light color: ________ Graph: ____________
Light Bubbles
distanc per
e (cm) minute
50
100

Mlatawan
Light color: ________ Graph: ____________
Light Bubbles rt II.
PROCEDURES:
1. Take a beaker and fill 3/4ths of it with pond water/distilled water.
2. Take some freshly cut elodea twigs and insert them in the nozzle from
below the stem of a glass funnel.
3. Keep this funnel in an inverted position at the bottom of the beaker. A
beehive arrangement is used in between the base of the funnel and
bottom of the beaker.
4. Now a test tube is filled with pond water and is inverted over the stem of
the funnel.
5. The whole arrangement must be kept on a flat surface under bright
sunlight for some time. (Alternative: Flashlight)
6. After some time, get a leaf from the set – up and mount it on the slide.
Drop a small amount of distilled water and view it under the scanner, LPO
and HPO.
GUIDE QUESTIONS:
1. Is the cell wall present?________________________________________
2. Is the cell membrane present?__________________________________
3. Is the nucleus present?________________________________________
4. What other organelles did you see in the wet mount?________________
_____________________________________________________________
Part II.
PROCEDURES:
7. Take a beaker and fill 3/4ths of it with pond water/distilled water.
8. Take some freshly cut elodea twigs and insert them in the nozzle from
below the stem of a glass funnel.
9. Keep this funnel in an inverted position at the bottom of the beaker. A
beehive arrangement is used in between the base of the funnel and
bottom of the beaker.
10. Now a test tube is filled with pond water and is inverted over the stem of
the funnel.
11. The whole arrangement must be kept on a flat surface under bright
sunlight for some time. (Alternative: Flashlight)
12. After some time, get a leaf from the set – up and mount it on the slide.
Drop a small amount of distilled water and view it under the scanner, LPO
and HPO.
GUIDE QUESTIONS:
5. Is the cell wall present?________________________________________
6. Is the cell membrane present?__________________________________
7. Is the nucleus present?________________________________________
8. What other organelles did you see in the wet mount?________________
_____________________________________________________________
Mlatawan