HEMATOLOGY 1 LABORATORY

Name: Calundre, Marie Kesiah V. Date: September 1, 2023

The Cells of Hematopoiesis

You learned the tedious process of Hematopoiesis in our lecture class.

To be more familiar with the cells of Hematopoiesis, complete the table below by filling out the missing details.
Include a drawing of the cell in the cell column after its name.

INDIVIDUAL CELLS OF HEMATOPOIESIS

A. THE ERYTHROCYTIC SERIES

Cell Size (um) Nucleus/Cytoplasm Nuclear Cytoplasmic Morphologic

(N/C) Ratio Morphologic Characteristics
Characteristics

Pronormoblast  Round to slightly  Dark blue; may have

oval prominent Golgi
12-20 um 8:1  Nucleoli: 1 to 2
 Chromatin: Fine

Basophilic normoblast  Round to slightly  Dark blue

oval
10-15 um 6:1  Nucleoli: 0 to 1
 Chromatin:
Slightly
condensed

Polychromatophilic normoblast  Round  Gray-blue as a

result of
10-12 um 4:1  Nucleoli: None hemoglobinization
 Chromatin:
Quite
condensed

Orthochromatic normoblast  Round  More-pink or salmon

 Nucleoli: 0 than blue
8-10 um 0.5:1  Chromatin: Fully
condensed

Polychromatophilic erythrocyte  Absent  Color is slightly more-

 Nucleoli: N/A blue/purple than the
8-8.5 um N/A  Chromatin: N/A mature erythrocyte
HEMATOLOGY 1 LABORATORY

Mature erythrocyte Biconcave  Absent  Salmon with central

(7-8 um)  Nucleoli: N/A pallor of about one-
Thickness N/A  Chromatin: N/A third of the diameter
(1.5-2.5) of the cell

B. THE GRANULOCYTIC SERIES

Cell Size (um) N/C ratio Nucleus Cytoplasm

Myeloblast  Round to oval  Moderate basophilia

15-20 um 4:1  Nucleoli: 2 to 5  Granules: Absent or up to

20
 Chromatin: Fine

Promyelocyte  Round to oval  Basophilic Granules:

14-24 um 3:1  Nucleoli: 1 to 3 or more PRIMARY: More than 20; may

be numerous; red to purple
 Chromatin: Fine, but slightly or burgundy
coarser than myeloblast
SECONDARY: None

Myelocyte  Round to oval; slightly Slightly basophilic, to cream-

eccentric; may have one
flattened side; may have a
clearing next to the nucleus
12-18 um 2:1 indicating the location of the
Golgi
 Nucleoli: Usually not visible
colored Granules:
PRIMARY: Few to moderate
SECONDARY: Variable
number; becoming
predominant as cell matures

 Chromatin: Coarse and

more condensed than
promyelocyte

Metamyelocyte  Indented; kidney bean  Pale pink, to cream

shape; indentation is less colored, to colorless
than 50% of the width of a
10-15 um 1.5:1 hypothetical round nucleus  Granules:
PRIMARY: Few
 Nucleoli: Not visible SECONDARY: Many (full
complement)
 Chromatin: Moderately
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clumped

Band/Stab  Constricted but no  Pale pink, to colorless

threadlike filament; Granules:
10-15 um Cytoplasm indentation is more than
predominates 50% of the width of a PRIMARY: Few
hypothetical round nucleus SECONDARY: Abundant

 NOTE: Chromatin must be

visible in constriction; may
be folded over.

 Nucleoli: Not visible

 Chromatin: Coarse, clumped

Neutrophil  2 to 5 lobes connected by  Pale pink, cream-colored,

thin filaments, without or colorless
10-15 um Cytoplasm visible chromatin
predominates
 Nucleoli: Not visible

 Chromatin: Coarse, clumped

Basophil  Usually, two lobes  Lavender to colorless

connected by thin filaments Granules:
without visible chromatin
PRIMARY: Rare
 Nucleoli: Not visible SECONDARY: Large, variable
in number with uneven
10-14 um Cytoplasm  Chromatin: Coarse, distribution, may obscure
predominates clumped nucleus; deep purple to black;
irregularly shaped. Granules
are water-soluble and may be
washed out during staining;
thus, they appear as empty
areas in the cytoplasm

Eosinophil  Two to three lobes  Cream-colored; may have

connected by thin filaments irregular borders
without visible chromatin;
majority of mature cells Granules:
12-17 um Cytoplasm have two lobes PRIMARY: Rare
predominates SECONDARY: Abundant pale
 Nucleoli: Not visible orange to dark orange;
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 Chromatin: Coarse, clumped appear refractile

C. THE MONOCYTIC SERIES

Cell Size (um) N/C ratio Nucleus Cytoplasm

Monoblast  Round to oval; may be irregularly shaped  Light blue to gray

Granules: None
12-18 um 4:1  Nucleoli: 1 to 2; may not be visible

 Chromatin: Fine

Promonocyt  Irregularly shaped; folded; may have brain-  Light blue to gray
e like convolutions
12-20 um 2-3:1 Granules: Fine azurophilic
 Nucleoli: May or may not be visible (burgundy colored)

 Chromatin: Fine to lacy

Monocyte  Variable; may be round, horseshoe shaped,  Blue-gray; may have

or kidney shaped; often has folds producing pseudopods
brain-like convolutions
12-20 um Variable  Nucleoli: Not visible Granules: Many fine
 Chromatin: Lacy granules frequently giving
the appearance of ground
glass
Vacuoles: Absent to
numerous

D. THE LYMPHOCYTIC SERIES

Lymphoid Size (um) N/C Nucleus Cytoplasm

progenitor ratio

Lymphoblast  Round to oval  Scant; slightly to moderately

HEMATOLOGY 1 LABORATORY

basophilic
10-20 um 7:1-4:1  Nucleoli: Greater than or equal to
1 Granules: None

 Chromatin: Fine, evenly stained

Prolymphocyte  Round or indented  Light blue

 Nucleoli: 0 to 1; usually single, Granules: None

9-18 um 3-4:1 prominent, large nucleolus

 Chromatin: Slightly clumped;

intermediate between lymphoblast
and mature lymphocyte

Lymphocyte  Round to oval; may be slightly  Scant to moderate; sky blue;

indented vacuoles may be present
7-18 um 5:1-2:1
Nucleoli: Occasional Granules: None in a small
Chromatin: Condensed, clumped, lymphocyte; may be a few
blocky, smudged azurophilic in larger lymphocytes; if
granules are prominent, the cell is
called a large granular lymphocyte.

E. ANTIGEN-DEPENDENT MATURATION SERIES

Mature B cell Size (um) N/C ratio Nucleus Cytoplasm

Plasmoblast  large and round  basophilic

 contains one or more
15-20 um 4:1 prominent nucleoli

Proplasmacyte  large and round to oval  may contain more RER and
Golgi apparatus
10-15 um 3:1

Plasma cell  Round or oval; eccentric  Deeply basophilic, often with

perinuclear clear zone (hof)
8-20 um 2:1-1:1  Nucleoli: None
Granules: None
 Chromatin: Coarse Vacuoles: None to several
HEMATOLOGY 1 LABORATORY

F. THE MEGAKARYOCYTIC SERIES

Cell (CFU-Meg) Size (um) N/C ratio Nucleus Cytoplasm

Megakaryoblast  Round  Basophilic

 Nucleoli: 2 to 6
10-24 um 3:1  Chromatin: Granules: Absent by Wright
Homogeneous, stain
loosely organized

Promegakaryocyte  Indented  Basophilic

15-40 um 1:2  Nucleoli: Variable Granules: Present

 Chromatin:
Condensed

Granular megakaryocyte  2 to 32 lobes (8 lobes:  Blue to pink; abundant

most common)
Granules: Reddish blue; few to
20-90 um Variable NOTE: The size of the cell abundant
varies according to the
number of lobes present.

Metamegakaryocyte  Irregular  Granules: scattered,

 lobulated -Up to 32 aggregated, clumped
lobes  Blue-pink
 this cell is very large  Fine azurophilic granules
80-100 um Variable with up to 32 nuclei

Thrombocyte  No nucleus  Light blue to purple

cytoplasm, very granular
1-4 um Variable  Fine azurophilic granules

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