Fo\) 24 q Counse Code: PHR 412 Title. Advon ced Pharmacedical Anal $\s Teacher: cMy Made b 5 q Sabekun Nahar Td | UGO8- 34-16 -063 Batch: 33 Section: Age OM Sin (Advanced Chanmacectica) D Nee _Prelysis) BPs é | Trtreduction to da Dav@lard medicines jane rwb isame In ehaninagecdigel eum. Drug io a chemieal substance that! ia helensegatie in nectene, wealy orgptie compound orl ured Aer Cune, preveshon, aind diagnosis cr dineaser « BI malice aaa daplalenibid nbblally Anmess/ansimedicines. mae ane actNe Substances uhene | Aanists entlpienta. ane mined ith medicine. ee re RY AG Hi RSBD Gorn fbi toncet be corcidenod jas dauc) the impordinee of [strildtune cleteremination in Pharmacy, Vb aiedpbelat « GR a mclccle and Whe Ainedtalde FL! Sit be Considered as tg { OM we dot know the, atuchine, "3 iclemveatfon le wot possible . Oe mare assag, sinuchine Is. \epecctart @® Phaamaceninetic and phanmaredunamie prope | athe Oma can only be. /ludied by wing the 4 mnuctune « @T deteamine the ee Sane a pointQe be rid) A SBOP) apps | a dnug + duvg minuclane eyo! Wendededty

62a5- 4000 en! eS: ea evo scen© wiley! Prerence of) ungatupalion. Per et Opec, PCE Buoattative eatimatony 4) 4), 3h Te the vo) ormpimda ang ame, arn bs Ideritied , Alrrost — cvzeles= “becaue WAL? « by Without NMA’ no rincicharal pectermance ean be dene, mass detergmt-nertion rasa Accurate be Nuslesin Majpnétia Resonance, i @ Myclear /Pheromencn Pelalems «Resonance “tneans (tio amengy source eo-opende end -\nemster happens. Bend vt pedi! happen | becatve + a? Fens nuclets haz prion ve) angi neutaon. Che mae ruclews in may beeatise of Sroton, Proton ard 2 is’ Aited’ | other vise’) a¥emio|/stidcland will be Gestnaed . Electnons (dan biuchrate Nme Spectroscopy) is a/) spectnoséopt e Ynethed Prot qty 3 Pinudleral tntoremation alte a “mele cule Basie principle dp MMA: @ AM nucleus ane brs) vely changed @ fome A the nuclei have —the aenaing ee The spin is mm on angular. tenhion@ OH Flay valle dhadnaa I a aT thik | @/ tea) chien OR eg aevelopir | changed parti cles behave "34 ’ he Vee) OG EN, cx north pole (N) and co soa Dia. | same. GElectnen and. pcan 50 ae ee ont Ge ) \ inthe middle a electron ill Yall} : 4 ee Me eatstence of an alam . Without '9e me be nme dlementhy Ta piri NoPiall "-nulelef Lidia a aCBl Mugla! cctHht 4, oI mW Way SPI are alte pur ie epumber (Ty ck greeters O exhibria @ When ectposed kal val lange / emternal jrragnetia Aye}d, they. eon priming ra j i Nave névenali , abtentatjods, One ct--)h is (mest clerely aAigned, othed is, leqath lesely aligned Caubtaned Opposite ONMA deals, oun the. abigned ty the pole). (raund Steche THransifton Amor -the mms} clase\y leat pisily lobed Leckctbed mich Gy MURR alc ness the Aberemy /of Read ig Whee T metnege ds. he pce Ted cuisHe | of. ong ae Prtebe by Absonbjn, JO en ay A PPimg '\9SD> Nebel prize (Alok, Aencelt) —- Bb Aneudhey!) al Inder (emerge! When piven, welll ae 1 pak and lca do Wentieation . | wavelength and energy ie imienerly pregon Nona, | Mdrmerekens calculi | Energy \+ found ooo Packet: Nuclear spin angina momenfun Isidetenmined by the Ac\low' gQuation! PaMucleat spin angular P=T +rPal “Mamerricen BRING 2. et ocerinen T= Sein ores qucetuym “ — Mumber Ghe'tl Spin and who'll not) y= Planks constant Mast number = Trlal proten+ neuhion Atemie 35 Proton umber ane T Mass mo, Mrmie) a. cE 1 Odd Odd | Eyen Eat ot (hols Ww ot” . tat Eyenie \2 (Fen | ¢ gs 'e@ Bienay odd 1, 25/3 BBW ey Ber iy When nuclein in placed inan eatennal maqnetic ANeld, the number de orientations can be ound b RDA) Yr, enamle, GH bar tas orlientecho: 1 ane (27+1) =o spins Ceimplest)Ce 4, Th + P= 0, the mumbed OE hele: + 1 t / : I, a HT tl” have! one cae | elk Hi oe’ ™ am ee r eR K | Uy lp reperyy \:{ | TA, pe bo cthen , valug = 2% etal att: numize cick Be ML 1a a, M, T= 1 5) onienkcton/mumbeal= 3 / 902/)D = On ty po,|sein , OPES! f TSO 'S ci stor! Oriemation and Sern . Ge ‘ ye y { Wer} | velte) SO te cote Hore, oremnie hn \ wey i chen 5@, £0 = i g ™e spin Property hig Tl i Nar oda oF ai > ae. 7 see oroperty Pest — onganta CMR (Canton m egretic resonance) CeNMe Pia Priston ince neta TeSEnadee)) Po Ne Nuel ee Precissional hon + / W \s i: hem aproon j= placed On, enteral! Pagnct Meld, i+ develops anther roel BY Mee | “motion induced) | Preciesional, motion ls)enesenh oo long sa Paces ye | Field ie auttehed. on, [Total spin = precission . | Presicsional melon ened is | equal to ot pe vien bh enenqy i= supplied, this ill q agaist the ao vhield « Whe» See is applied ae Arm ob radi ote Lencitet the motion incnearen, and Hips “ty, become anti When ene rey is cumoteds i Kies acute “the origina Position (aalanadtion):, The Aluclkatien 1 Moanelie Held ascochated)! ’ tin the — culaaation \Yoroness js Called eronance The raloWonshie between ele clnemagnetic Frequency end Magnelia Weld stnenpih is shoen by the Lanmoi ie x 4 equaction| py = S80 Ve a oe J | v= Ryerss ets a Coins 1. 26450) neVia Field, (ey Narciable) / Maori gr iT), | @ > Shera th oka PaBiedt recy v= tresuency in He The: Mey nequined 7 Alip_ the! precloyy dépendy oy the rr neath ch the get Aid a the SATE g erst the magnetia | Held! “hel g cect ove the esere of the ~nuclel to elain the oni ent oy. Me, 4E = We higher Fretueney cadierhi on Loould (be reguined “for the tie. Vie Po (| Sp ead ot arena cr Ns C ’ S\ 4 | weGo Mie iv aN ny=G0, S aM og ey) ee (Dy? ny z Pe As Wee absonoHon ' a tigalng iBasie princidle tor onal Oe Bis) tots ae br @Mereten’ plat a! oan | tetas sa orgula mation) le When exposed to a external wegjactic Ae\d . preter | develops sdgpring ‘Me high eneney Slate Esproning + | deraeings) which) is called, pageissional meton) @ When every is aitindaauth, the/enaten telrns be salanect on, de A Loy =) angular menor = Be 499 rege O prreejesjonal oa 1 Yo & Bo then o= VD—s nuclear Wreanetia Retonidnde deeur Bowl) require “mone srengy amd none preciesio | motion -t> Incneme the strength of the held « Chemical shitt : BEEN ictal! hate (dvterent Mocealecleral, -< once il) aloszonlo ditbenert neal Why didterent preecissional rmction | Unden -the indluence oh a mnaginetie tied. electron Cimeuletes in o ae being negative charged pried® Di |Fagehit ett arctan called) secondary “er | “elds het oppesgns the; applied, rangemetig “SF uf : a e mela “eld. |-the erst eapmaiences ditteremt Cory hi So, egnstic j Pree ata bed tHe she Heo: me seemdary “mMeoy: the xt mee the q a More the — applied mogmelie “ANeld ie 4 stheetve memehia “held eapetien dad’ by the Paston “1s 4 applied! aneameta Ae ld aAgeondansy moegnetiatield , Het” y prodding thielded teriom the applied wmesietia eld We can say Tr emty, is, ao Comeatale Mem Lig bonded Hmm, So, the Phared electwn is dao more ye chmiFatsahed! Ha andl ye ledkieh (s clectne nea btive, Proton sill have less ethect ove tho elechuin , ¢ te the othe mw The ne Ds Tete Pcouns, TH some part eala Coven , 2 “ WF ies: Gir iy ie Tara eleotron Fermereea “TH! k | doeon't op pce’ reclhe {2 Megective shielding , Prshn coil empenionde Mone mene tc bield Ahern the apolied megnetio! teva,o Modiied — Larmor. equation, , Hci ot OA Ae 8. ae : vw a ae pret on Brn ein a |/ PagM stem Oi’ L9 Gp Oe hae Pd AIO , an a Bia a tek 9 oe VOT Re nelio Ateld 4 | A=0- never (ro 7» ro shielding) “> Shielding cannot i] | i been. 60, % hare be between’ D and 1, |neto arene = Pera mens Held . chele equation 16 Zeno Which smeome no | yregynetia Ups a+ ss eppezing §— eversrthing. So it caret be 0 and it. 4d oF ic he i Effective 3 V xX Bo Frieling higher a Magnetic Aiea | v a he au 2 | xe \ | rape, Wien | Pray Wee 4 Bedhie Wing ‘ Feng Prckn in ao Molecule is present in bond Mone clectnome ative compolind —Warded “With he proton ci l\ pull the protns e wand feet e density col deereare “trem! 6 Ast eau a sThis is! called ce sla low > density > Panay 3) > etrect i € om Pact | O more ehtreetive Meenete Aieva @ high enecissional motion ® Wah peur uy® i aT 1 Yer ae BY 7 ih | Destinition of chemical . ~iny He Ahem 0-H, call lake Morg “10g: aa i}!s Teta. Here | Cann, ‘acing a high elkeclive a) th Som e Patt eulan Raya Pleetnon | \hary Relfores,, Called Negechive *Nelding,. doeon't 4, Ls Meclhe ¢ 3 . re os THis $5Eoshay Wiepend. ‘efh/ action depends om \ Ray ear quad elie Go = 2400 gam — oy 5 fo OM Hania Bed asyet eral! ayeadhs ergs i TA. Milnes 1) Solid COrctetn) past) ance Kee |B Schon Seectnams —_auseensen@ | Biter diss\ying the sample Ina sowen}s “awe TY in | Fy a NMR tube ~ a Vea | Saree (2- ye By on jest) Game damevere Och, chould be wed 0% Pavenh oo IY thee m0 prskon, the meduce> “he possibility of interruption - Gu} not A\\ compounds disselve in Cely , Sa, adel, Ye Wred \mstead of CHels 0 athe ersten and ther, 1H Mott ave etter on spcetn Thess, watt) be “mace amounh ch) peak Ubhich ae} be a issue, Phe imbennal standand TMS (Teh Methy Zilane) 1¢ teed “te detenmine the enact amount of main Cle) bred ¥q QApnreby ,—— Le “1m (uneasy | | tog 6! et / ne ¢ Hie) TH je not gesetble fh wecane oblate n° | Oreciely the amount Ce energy jabrembsd) 1 33 \ndivi dual | proton i derbi eoompastay of) | | TMs"; r Bt a mefenence compound Lied, darn TMeamunemernt ch mesonance of vorimple + So that “fhe Rersenance ct prelons ct each sample is pas relative +o thet of Prichne ch reberenee compound Advantage. ams: | ce \ Ot ie a \datile vnhuill Boiling leainb ts a+" Mineide with mos} ob the organic, eeNentay. And ete to He Polling point. i is hae Carcpovinlo¥e OT has! do! ohshos .- There 12 Protas ane hgn\y shielded’) di Z}ttoon hy eleehs posh ve shee oN WL ca a hare singlet » AW photons dud Cherhieally equivalent. No oranie Campou f ($ mone phielded Nan TMS. So j-+he Pean ft “tp 13 -taken areas ‘0! Vins) =oOM deo wh ; ory sony or chem cally) colt the samp lel wet rr tet, ts tment, Simelenselt vey tama ce : @zampe can be recovered ae rey 1 hal Fame sever can be ured Tene Himes " Main edvantege : 4 , [The aly ebecneay happens inl Me Cmagatis) unit The Machine cannot cletermine thas entacl amount but the newt} 1% visible am Wo. We suogly Saga in MYW2> “rms aves in MY\2 1 but subsinaction OF TMS Value. giver) she meray in He Grad 1D = Ver Vrms = He (ber, ve ny vn) 1 share sing let by +the12 protng OF Weal. 400 He peétons transtormeahton 19 eer, _ NSE ees M He Fung rer? He (Heid depend ent) Ppm Uield’ independent) > O +710 —-7 AS PPM a eal mum mange mart foe ee se nieldirg sae ed@ . g a vw ( Pectong afieclingy dpemiecd Shite 17) 1! JA, Totrameleculare Arachne /) i) nt oy AD Trduchive effect, pf 2) Anisctnope, ok cheeniéal looride Dan den” Waals) deshie ili ; Aiea B-Bttect a atlvert ; concentration and —fempenachure ye © Mretnecen loading ig frerorel LAMA ay aia 9 "OT pte RE / [ 1 an I nsec Bn Lp sedative Eheim This eltech is dueto the nalane cthe group ctached +o an atm, and is ebrecte Abraugh bende. TH Is | enpenienced be Pre | Ugh & bonds the Austhen +} gee (i odkte re Skbend, | the lece eftechNe) tH tic, f “Thrsvaty lerndst; 4 bebe ) got =: \sa)P CHels os PEM, TRG | 2.90 Mala Ly els) AS Si cau pel Natt | 2129 Jelena, Staal ps age PPM neqatinih O- eb, 20 q Protons ane, ret pete ginny elections | bu} Ihnough leonds Vike Im indict on ‘ Method ,Toiuctie clit ie cern : _ deshicWing, ie 2a. fe Fite ei Hi PrGh> oo oem HF 1 WA et, OO eas ®@ @ } and unedhected we came aa Md i. rae r “{ rd j Paaten @ mos} deshielded i Ce A Upto @ wo attest cv induchve * a v vali bond . When pera ie qrne ainda © the closes} ectem ,pails the pasion, pis. mesh debielded 22” onelaen BMY conde HS - | Highe the number ch” Algehnenigerive oustyuentns | higher the deshielting ebtect| 3 , td * is an eae a caleded | “Neck mane hie Ae qionny chick’ cabibit ; in dithenenh lecnaste neqions « 1) Hi ep tae a? ee Msanctia Aield an Sane! of! sloutle , bonte compact thi MMA SESE Bete booth crmmpacont al Shield caw) Bel Sttended pay ®, 5-@ RAT ed da, 1) Deshi ea, id oI q Peo oy | 4 —Tniple bond compan da HR “te Mires Can be extended mi. ih: < aies ' & ‘4 ' ) 7 eta Detieuing A\iulo P cal ai mah ea aH | ! ae Hi Felt ® Acetylene praton © eneSP ae a fen ee ctio > 95+ 4b ppm eo °o Cech + 6-6) pp’ Czen 4 a.5- a ceca Gen > a8 ppm, Bre watie aoup + eels : ! : ON is the imtentaction That! occurs between Mee oe Neighbouning | fen “equivalent prefers » Fitivaent] Peak will an al single peak +52. the protons, +i Bi ei 14 ak MAS, v - Singleh | Dourlet ya pet, Guaqter).. Mmatvele, Raha) Solit munnen Peak will ae SsAib Urthene is mon- eduivalent prison. Celts, Wee ~ ely h wt 41 so44 Slag Ssh nember Manimum Dumber! of cote Nga He - ct Ag m2 = WL equivalent paston Equivalent — pachns ae Sing et peak. Non equivalent Prehns will show a Osa This split a ape) \s Known as Spin~ spin oeDNisinal easel TH ts the Petey cohich Ane sepagated be) athe ched rag “ahe | deed iel a chms, H \ 74 mS e ii 7 ee , a a valet Corea and i A “es 1 2) Gemina) ¢ ae SNS LE a ale ete oa ot Mon - ELI Valent Pristoms atte chedi ly the (aun Gane Gnd ls seperated ye bonds. Vi - Be eel iy IN | J Pines la TTR Ae GH 3) Aromatie aduplin ; Bilin Wea Tee a Veli 4 Deedes Jonths 7 +-9 Ha “petals | meta = 2-3Ha "3 (pana) Trane = 0-4 He 4 (onthe) SJ) valies ane ary Contbant and ane he. in ouara wh pe determi nettn ,Moan ps CGY hte slay. a a ee Mg leis = 3-4 Ha Covenge=42H2) 5 “Hines =a se a od > ae We (avenoge: = 46 H2) We F Valwe ! (a i Von ah)! ae is lini RY 1 This happens (between prictns Mhat ane Sepanated ‘4 eg nord’ ds? oe eaptite PSE & a Wi by Anew valtyli Ws Hp then heme alga 4 Wa —c N yo7 Yo es a a ‘ ty, ew) Biz > vicival capling 2 > allylic encelin 4,45 4,4 — heme allytie eneling eea) Tae tena on the Sie deal angle X ¢ 5, ¥, lelectra etl th ‘albaaan wi ‘ 4) fot Vy toe eva cangle: r eeu bred ~ait Lie © seal, ay, Dihewe| Se hstnel angie : - lt: DH is the angle beheeen cite Nydacgen hes, dohnected “ates Pa anata why Ceanten) | rp Caw be oetig egy ae FEOF. Karp Situation dia tate, Mg midde A vicinal he i Seuelinas UAnstnamed bh ; yt Elect mene ative bs htu eyatl ORY ER ne Con stra ad Coune, amivbie ont, per. fan bay ons ,“Age s ; Di = em eA irs 3 * pee P He AE ree lean! 6 Hebrew Moniwe Bee) ¥ o 0° ((teatlol Fade) Whew, Pm oe oost = 9 Wes 0188 Se er ch Ie it : amount ase be fag As $0 ‘no aT i PHO" 5 cos? g a4 ae! Be —012@ =8.22 parma) @ = 160 + Cos Téo" = 1) igen ans al oe ae . 2 2 mt Oo ene! is no emupling ut tt cant b e Said shat theme's no Proton el (ae Nateal Amourct Sample Needed ane Mo: Conaliney ‘chemical shitt range Tokgpetio Jae ‘Ohaus eed PME and AMEE a SSS ee abundance | abun, ent | Pretoten. Magnehe | Pesomarn ce s ‘ | 29.81, on 99.94, # | Less sample is needed | [Cebu Aols ma) PR Fast Poecess (ees tne) | 6 [40 minutes re miny) ~~ 3Neuna (aa hom) i inn Covelirgy sccand Getg- + PP™m: Most & the Prishn between #9 cen Neo = nee (ooo mHa)-m a (weaterd - ae) | Canton, Magnet | Retonance » y aden Needed | (about 20 ™) i Mone Sample is eco Ortoaess y 250 MHe No couthngy nes Cre, beck We | x ak ay vans a 25 He Sentai Sega ¢-4 0-220 ~228 pom a) No xx