LDLR1

SynGene GeneTools - File version: 3.08.01 - Serial No.
14141*11717*labfo*mpcsdq
SynGene Laboratories This heading can be changed by the user.
Image document data
File C:\Documents and

Settings\user\Desktop\
Warin\ldlrall.sgd
Created by lab
Generated on Wednesday February 27 2008 11:26:23 AM
ID 1204086383
Black:0 White:3999 Gamma:1.00

Properties
User Field 1
User Field 2
User Field 3
User Field 4
User Field 5
Capture
Digitizer G:BOX Chemi HR
(MOT) - Gel
Sample
Camera Factory EtBr/UV Trans UV lighting motorised lens
configuration
Settings GeneSnap
version 7.01.02
Exposure Time:
0.100 seconds
Frame
accumulation: Off
(Stop on
saturation: Off)
Darkroom camera
filter: EtBr/UV
Darkroom lighting:
Transilluminator
EDR: Off
Camera
Resolution: 1.38
MPixel (1x1 bin)
Camera Serial ID:
Q18677
GrabberChain
User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

Signature :
SynGene GeneTools - File version: 3.08.01 - Serial No. 14141*11717*labfo*mpcsdq
Image
resampler
StepX 1
StepY 1
Quantage
Dynamic Range
Extender
Bypass -1
Equalize 0
ExposureRatio
2
Exposures
4
GaussianEqualiza
tion 0
MaximumIntensity
Fraction 0.9
MinimumIntensity
Fraction 0.1
MinimumValidCou
nt 1000
Type 2/4
Darkroom
CurrentFilterID
14
CurrentFilterPositi
on -1
FBLens 0
FocusPosition
-1
IrisFNumber
-1
ZoomFocalLength
-1
Camera
Calibration
bBackgndCorr
1
bStarfldCorr
1
corCameraID
"Q18677"
corFilePath
"cq.cor"
QCam
AbsoluteOffset
0
BayerToRGB
4
Capture8Bits
0
ExposureTime
0.1
Gain 1
GainBlue
-1
GainGreen
-1
GainRed-1
binningMode
1
cameraType
7

Signature :
Document history
Date Author
Wednesday February 27 2008 2:25:51 PM User
Integration parameters
Baseline correction method LOWEST SLOPE
Baseline correction offset YES
Color filtration options NO FILTER
Smoothing SAVITSKY-GOLAY order 2 width 3
Type Fluorescence
Image dimensions 1392 x 1040
Electrophoresis direction Down
Minimum peak height 3
Minimum peak width (pixels) 7
Minimum peak area 1
Track 1

Signature :
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Rf distance down track
Profile height
2000
4
1500 5
7

1000
8
500
1 2 3 9 10 11
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 1
Number Mol. weight Height Raw vol.
1 0.00 157.822 125635.92
2 0.00 175.316 156982.39
3 0.00 193.871 133514.55
4 0.00 1611.142 955620.94
5 0.00 1441.990 1087193.00
6 0.00 2207.859 1438731.50
7 0.00 1362.940 1103047.13
8 0.00 808.572 785781.56
9 0.00 184.542 98882.51
10 0.00 193.965 165030.69
11 0.00 158.391 84292.20
Track 2

Signature :
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
2000
1500
8
1000
11
500
5 6 7 9 10 1213
1 2 34
0
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 2
1 0.00 97.714 62699.00
2 0.00 124.582 88072.38
3 0.00 127.210 150919.91
4 0.00 134.094 57114.39
5 0.00 182.743 313962.13
6 0.00 192.796 93215.53
7 0.00 181.564 78548.93
8 0.00 1193.227 1057896.25
9 0.00 180.566 67991.58
10 0.00 189.246 79519.64
11 0.00 549.314 664717.69
12 0.00 217.708 370789.72
13 0.00 187.200 96932.35
Track 3

Signature :
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
2000
1500

1000 10
13
500
1 9 1415
56 7 8 11 12
2 3 4
0
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 3
1 0.00 248.502 72309.94
2 0.00 69.046 134944.05
3 0.00 88.061 68823.27
4 0.00 110.895 65728.52
5 0.00 147.373 114951.79
6 0.00 153.222 82275.91
7 0.00 174.872 75530.92
8 0.00 198.304 62884.89
9 0.00 209.942 163751.19
10 0.00 967.629 946677.63
11 0.00 191.139 81685.35
12 0.00 197.874 64488.21
13 0.00 910.273 1162193.13
14 0.00 247.605 477666.69

Signature :
15 0.00 213.230 112557.88

Track 4
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
2000
1500

1000 10
500
1112
3 4 56 78
1 2
0
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 4
1 0.00 92.885 90663.49
2 0.00 117.774 86941.23
3 0.00 163.362 176236.36
4 0.00 215.741 130177.02
5 0.00 215.872 101228.05
6 0.00 244.567 182653.94
7 0.00 199.463 75816.05
8 0.00 205.933 99173.44
9 0.00 2388.020 1990778.50
10 0.00 951.132 1112925.38
11 0.00 264.330 393595.47
12 0.00 241.447 127100.23

Signature :
Track 5
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
2000
1500

1000
9
500
5 6 8 101112
2 3 4
1
0
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 5
1 0.00 109.603 96395.77
2 0.00 127.040 106061.95
3 0.00 142.643 75737.53
4 0.00 179.881 196407.36
5 0.00 251.795 353705.28
6 0.00 247.290 93323.37
7 0.00 2366.663 2047513.13
8 0.00 221.581 128647.66
9 0.00 843.841 997537.69
10 0.00 230.138 155398.73
11 0.00 267.326 363519.34
12 0.00 240.418 125223.37
Track 6

Signature :
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
2000
7
1500
Rf distance down track 8

1000
500
4 56 9 10
1 23
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 6
1 0.00 125.681 89788.38
2 0.00 170.348 79256.93
3 0.00 170.857 90461.99
4 0.00 225.042 184174.91
5 0.00 253.346 234992.77
6 0.00 306.435 141960.81
7 0.00 1561.403 1462570.75
8 0.00 1059.168 1390019.88
9 0.00 276.004 398121.53
10 0.00 261.974 137151.83
Track 7

Signature :
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
11
2000
1500
14
1000
500
6 7 8 9 10 1213 151617
2 34 5
1
0
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 7
1 0.00 103.876 87827.61
2 0.00 149.470 232543.36
3 0.00 167.948 172722.78
4 0.00 190.716 135896.56
5 0.00 193.626 123687.91
6 0.00 227.509 134335.84
7 0.00 267.211 149899.45
8 0.00 265.862 143423.08
9 0.00 254.326 95694.35
10 0.00 264.679 182793.69
11 0.00 2770.936 2210472.75
12 0.00 231.752 100768.41
13 0.00 249.950 132805.22
14 0.00 1189.669 1432898.25

Signature :
15 0.00 250.959 146606.47

16 0.00 297.069 287362.41
17 0.00 251.784 128673.49
Track 8
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
2000
1500
Rf distance down track 7

1000
500
3 6 8 9
2 5
1
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 8
1 0.00 193.484 103549.73
2 0.00 254.433 260355.09
3 0.00 304.108 234164.66
4 0.00 2550.178 2155416.75
5 0.00 228.040 98664.36
6 0.00 260.563 224447.16
7 0.00 1004.978 1214403.75
8 0.00 300.672 513554.31
9 0.00 250.470 137687.66
Track 9

Signature :
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
2000
12
1500

1000 15
500
2 1617
1 78 9 10
11 13 14
3 4 5 6
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 9
1 0.00 265.829 104255.57
2 0.00 351.043 89691.80
3 0.00 126.700 110712.45
4 0.00 135.815 122226.00
5 0.00 160.252 221200.00
6 0.00 178.700 114320.93
7 0.00 223.830 177461.52
8 0.00 228.245 99058.63
9 0.00 264.738 179697.50
10 0.00 244.698 106599.26
11 0.00 246.182 120694.75
12 0.00 1812.545 1453970.25
13 0.00 230.253 87734.34
14 0.00 251.589 187273.69

Signature :
15 0.00 973.889 1159845.75

16 0.00 296.067 557048.75
17 0.00 249.065 126689.58
Track 10
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2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
2000 9
1500

1000 11
500
45 6 7 8 10 121314
1 2 3
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 10
1 0.00 127.554 77811.45
2 0.00 157.934 268779.78
3 0.00 176.548 76477.84
4 0.00 229.966 236571.84
5 0.00 227.042 86676.52
6 0.00 266.010 261199.75
7 0.00 245.005 79683.73
8 0.00 259.003 98235.07
9 0.00 1896.555 1451658.13
10 0.00 233.468 100938.68

Signature :
11 0.00 972.034 1084958.13

12 0.00 243.697 78390.12
13 0.00 289.063 356364.38
14 0.00 213.915 78306.23
Track 11
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
2000
1500 9

1000
12
500
7 8 10
11 1 4 15
13
1 3 4 5 6
2
0
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 11
1 0.00 196.286 74076.27
2 0.00 96.683 184912.44
3 0.00 158.716 150662.59
4 0.00 179.594 109054.37
5 0.00 199.759 72193.95
6 0.00 215.419 242509.94
7 0.00 250.228 125636.17
8 0.00 245.602 169126.33
9 0.00 1454.537 1189765.88

Signature :
10 0.00 234.413 162766.30

11 0.00 238.296 77368.31
12 0.00 798.824 993529.81
13 0.00 241.209 77722.75
14 0.00 240.491 77506.80
15 0.00 296.580 534140.75
Track 12
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
7
2000
1500
8
1000
500 5
4 6 910
1 23
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 12
1 0.00 113.106 100465.93
2 0.00 147.015 197520.67
3 0.00 153.520 89566.30
4 0.00 230.881 261035.86
5 0.00 377.484 370701.13
6 0.00 261.315 139204.48
7 0.00 2010.977 1521193.38

Signature :
8 0.00 1121.723 1361598.88

9 0.00 279.421 183571.72
10 0.00 293.041 94972.63
Track 13
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
2000
1500 8

1000 11
500 7
4 5 6 9 10 12 1314
2 3
1
0
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 13
1 0.00 95.305 71232.27
2 0.00 160.888 93485.97
3 0.00 177.503 240534.59
4 0.00 205.122 160379.94
5 0.00 237.456 166155.56
6 0.00 221.307 83300.55
7 0.00 394.497 302962.72
8 0.00 1398.252 1017059.69
9 0.00 200.278 108069.26
10 0.00 204.803 78364.71

Signature :
11 0.00 924.658 1202132.13

12 0.00 222.982 156080.63
13 0.00 280.264 372200.00
14 0.00 206.637 78693.66
Track 14
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
2000
1500

1000
500
4 56 7 8 9 1011 12 13 14 15
1 23
0
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 14
1 0.00 65.505 59068.16
2 0.00 120.401 106356.45
3 0.00 124.882 53928.98
4 0.00 146.305 55318.02
5 0.00 164.475 70501.06
6 0.00 164.010 53458.87
7 0.00 172.129 55178.52
8 0.00 185.157 87780.73
9 0.00 196.538 92965.30

Signature :
10 0.00 240.046 194599.55

11 0.00 186.378 80402.74
12 0.00 188.691 82015.95
13 0.00 201.556 65597.36
14 0.00 246.518 248233.28
15 0.00 195.352 75324.67
Track 15
Profile height
2000
1500
1000
500
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Profile height
2000
1500

1000
500
12 13
3 4 56 7 89 1011
1 2
0
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
Track 15
1 0.00 91.810 60437.63
2 0.00 105.450 127093.31
3 0.00 126.866 197696.63
4 0.00 137.104 66697.74
5 0.00 146.181 71422.69
6 0.00 153.473 114377.67
7 0.00 155.891 58936.96

Signature :
8 0.00 172.347 74154.79

9 0.00 169.750 82992.66
10 0.00 181.489 148082.77
11 0.00 185.670 131452.14
12 0.00 187.861 81591.95
13 0.00 238.135 693585.88
Molecular weight calibration details
Curve type Log piecewise linear
Distance measurement From Rf baseline
Propagation method Interpolate between standards
No molecular weight standards are defined !
Quantity calibration details
Curve type Linear through origin (single standard value)
Calibrate All tracks to a single curve
Units
No quantity calibrations are defined !

Signature :

LDLR1

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SynGene GeneTools - File version: 3.08.01 - Serial No.

Image document data

File C:\Documents and

Black:0 White:3999 Gamma:1.00

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

Rf distance down track

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

Rf distance down track

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

15 0.00 213.230 112557.88

Rf distance down track

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

Rf distance down track

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

Rf distance down track 8

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

15 0.00 250.959 146606.47

Rf distance down track 7

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

Rf distance down track

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

15 0.00 973.889 1159845.75

Rf distance down track

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

11 0.00 972.034 1084958.13

Rf distance down track

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

10 0.00 234.413 162766.30

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

8 0.00 1121.723 1361598.88

Rf distance down track

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

11 0.00 924.658 1202132.13

Rf distance down track

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

10 0.00 240.046 194599.55

Rf distance down track

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

8 0.00 172.347 74154.79

User: User Created: Wednesday, February 27, 2008 2:25:51 PM ID : 1324291806

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