Portfolio Overview

3M Petrifilm
3M Clean Trace Hygiene Monitoring
3M Molecular Detection System

December 7th , 2017

Mathias Boldt, Scientific Marekting & Professional Service Manager
mboldt@mmm.com
 A broad portfolio of solutions.
3M Food Safety
Quality Indicator Testing Pathogen Testing Sample Handling / Media

3M™ Petrifilm™ 3M™ Molecular 3M™ Sample

Plates Detection Handling Products
System

Hygiene Monitoring End-Product Screening Allergens

3M™ Clean -Trace™ 3M™ Microbial

Hygiene Monitoring Luminescence Introduction
System System Q1 2018, EMEA

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 Quelle: Pampers

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 3M™ Petrifilm™ Plates Quality Indicator Testing

Ready-to-use plates
3M™ Petrifilm™
Plates

product testing

environmental testing

Compliant to EU VO 2073
Compliant to ISO 11133:2014
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3M Petrifilm

Plastic film
Adhesive + Indicator

Cold water soluble

gel
Standard nutrients
adhesive
Plastic coated paper
printed with
counting grid
There are just four steps needed

1 - Inoculation

2 – Spreading

3 - Incubation

4 - Reading
3M Petrifilm Portfolio
3M PF Aerobic Count 48h
3M PF Rapid Aerobic Count 24h
3M PF Enterobacteriaceae
3M PF Coliforms
3M PF Select E. Coli
3M PF E. Coli/Coliforms
3M PF High Sensitivity Coliforms
3M PF Rapid Coliform 6h
3M PF Yeast & Mold 5-7d
3M PF Rapid Yeast & Mold 48h (validated)
3M PF Lactic Acid Bacteria validated
3M PF Staph. Express 24 + 3h

3M PF Aqua Heterotrophic
3M PF Aqua Coliform
3M PF Aqua Enterobacteriaceae
3M PF Aqua Yeast & Mold
Validations
3M Petrifilm Reference method AFNOR Validation

3M™ Petrifilm™ Aerobic Counz ISO 4833:2003-06 Yes

3M™ Petrifilm™ Rapid Aerobic Count ISO 4833:2003-06 Yes (Dairy)

3M™ Petrifilm™ Lactic Acid Bacteria ISO 15214:1998-08 Yes

3M™ Petrifilm™ Coliforms ISO 4831:2006; ISO 4832:2006 Yes

3M™ Petrifilm™ Select E. coli ISO 16649-2 Yes

3M™ Petrifilm™ Staph Aureus Express ISO 6888-1:2003-1, 2 Yes

3M™ Petrifilm Enterobacteriaceae ISO 21528-2: 2004 Yes

3M™ Petrifilm Rapid Coliforms ISO 4831:2006, ISO 4832:2006 Yes

3M™ Petrifilm Highsensitivity Coliforms ISO 4831:2006, ISO 4832:2006 Yes

3M™ Petrifilm Rapid Yeast and Mold ISO 21527-1, ISO 21527-2 Yes
 3M Petrifilm Innovations
3M Petrifilm Rapid Yeast and Mold Count
• AFNOR validated for 48h (60h) results
• Easy differentiation of yeast and molds

3M Petrifilm Rapid Aerobic Count

• AFNOR validated for dairy products

3M Petrifilm Lactic Acid

Bacteria Count
• AFNOR validated
• no anaerobic equipment
needed
• differentiation of homo-
and heterofermentives

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3M Petrifilm and environmental testing
Facts and Figures
• International validations
• high reproduceability, consistent results
• 18 month shelf life
• high productivity
• new rapid methods allow early read and
results • high flexibility – tests can be repeated immediately
• results are comparable to reference methods as same
methodology is used
• low usage of consumables
• easy automation - but can always be used without
hardware
• no media preparation necessary
• second source of supply in Europe
 Because you use Petrifilm versus traditional agar:

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3M CleanTrace
Hygiene Monitoring
 ATP Sources or
what should not be present after cleaning?
humans & animals!!

Microorganism
• bacteria
• yeast & mold

Food
• fruits Extra Terrestrians (E.T.)
• dairy products
• salad, vegetables
• meat
• etc…
 3M™ Clean-Trace™ Hygiene Monitoring and Management System
The complete system:
• New lightweight luminometer design
with user-friendly touchscreen and
one-handed operation
• Test Swabs
• Powerful software

• eanbles total hygiene documentation

• ATP Surface (measure)

• ATP Water total/free (measure)

• Visual Inspection (document)

• Temperature (document)

• pH (document)

• free defined tests…

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 Power of data
Immediately identify cost savings
Identify potential increased costs
• Detailed view at a sample plan level
• Visualize trends
• By sample point
• By particular day or week
• Easy to interpret
• Align resources
• Justify resources
• Justify new resources

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 Master Sanitation Schedule

Immediately know where to align your limited

resources
• Detailed view at a sample plan level
• Quickly identify locations of current and potential issues
• Visualize trends
• By sample point
• By particular day or week

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 Advanced technology.
Simplified.
3M uses advanced technologies —
Isothermal DNA Amplification and
Bioluminescence Detection — to bring you
the 3M™ Molecular Detection System for an
accurate, reliable molecular solution with
increased productivity.

Colruyt Group, 13th November, 2017

Mathias Boldt, mboldt@mmm.com
2
0
 What do our customers need in a pathogen system?

 Faster time to results

 Fewer false positives
 Easier to use
 Fewer transfer steps
 Regulatory approvals
 Intuitive software
 Robust hardware

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 Introducing the 3M™ Molecular Detection System

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 Simplified for productivity
3M Method 3M Method 3M Method 3M Method 3M Method
Salmonella E. coli O157 (including H7) Listeria Listeria monocytogenes Cronobacter
37°C ±1°C 37°C ±1°C
or 41.5°C ±1°C 41.5°C ±1°C 37°C ±1°C 37°C ±1°C 18-24 hrs
18-30 hrs 8-24 hrs 24-32 hrs 24-32 hrs*

*Raw dairy matrices

require 40 hrs.

Transfer 20 µL enriched sample to lysis tube.

Heat 15 minutes – 100°C ±1°C.

One
Cool 5 minutes on block at room temperature. Protocol
for All
Transfer 20 µL lysate to reagent tubes Pathogen
containing lyophilized pellet.
Targets
Place tubes in instrument.
Start run.
Amplification & detection in 15-75 minutes.
Automated & color-coded real-time results.

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 The next-generation of 3M™ Molecular Detection Assays

Introducing our newest assays:

3M™ Molecular Detection Assay 2 – Salmonella
3M™ Molecular Detection Assay 2 – Listeria
3M™ Molecular Detection Assay 2 – Listeria monocytogenes
3M™ Molecular Detection Assay 2 – E. coli O157 (including H7) • New chemistry based on 3M • Intuitive user interface for quick and
nanotechnology easy setup
3M™ Molecular Detection Assay 2 – Cronobacter • Unique color-change for increased • Real-time results help make critical
control decisions faster

New chemistry.
Faster time-to-result.
One protocol for all assays.
Streamlined workflow – now 30% faster. • Advanced isothermal technology • Compact instrument fits in any lab
overcomes limitation of older PCR and • All assays can be run simultaneously
antibody-based systems for optimal efficiency

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 3M Molecular Detection Assay 2 Protocol
Next Gen

Improved ease of use – 37% less handling.

Streamlined workflow – now 30% faster.
One protocol – all next-gen assays.

00:20:00

Advanced technology.
Simplified.

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 Mathias Boldt
3M Food Safety
Email: mboldt@mmm.com
Tel.: +49 170-7376809

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