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BIOCHEMICAL ENERGY PRODUCTION

Activity Number 1

Krebs Cycle (Citric Acid Cycle)

Direction: Write the CORRECT answer/s before the number.

1. Name the enzyme responsible for converting citrate to isocitrate in the Krebs cycle.
2. Identify the compound that serves as the starting molecule for the Krebs cycle.
3. Name the two carbon molecule that combines with oxaloacetate to initiate the Krebs cycle.
4. Identify the location within the cell where the Krebs cycle primarily occurs.
5. Name the two high-energy electron carriers produced in the Krebs cycle.
6. Identify the first NADH-producing step in the Krebs cycle.
7. Name the enzyme that catalyzes the conversion of succinyl-CoA to succinate.
8. Identify the number of ATP molecules directly produced in the Krebs cycle per glucose
molecule.
9. Name the compound that combines with oxaloacetate to form citrate in the Krebs cycle.
10. Identify the number of carbon dioxide molecules produced per turn of the Krebs cycle.
11. Name the enzyme responsible for converting succinate to fumarate.
12. Identify the two-carbon compound formed during the conversion of alpha-ketoglutarate to
succinyl-CoA.
13. Name the compound that combines with acetyl-CoA to form citrate.
14. Identify the Krebs cycle intermediate that can directly donate electrons to the electron
transport chain.
15. Name the enzyme that converts malate to oxaloacetate in the Krebs cycle.
16. Identify the step in the Krebs cycle that directly generates GTP.
17. Name the compound that exits the Krebs cycle to be used in the gluconeogenesis pathway.
18. Identify the enzyme that catalyzes the conversion of fumarate to malate.
19. Name the coenzyme that participates in the conversion of isocitrate to alpha-ketoglutarate.
20. Identify the final product of the Krebs cycle used to regenerate oxaloacetate.

Cellular Respiration
Direction: Underline the correct answer.

1. Which process occurs in the cytoplasm and does not require oxygen?
A) Glycolysis
B) Krebs Cycle
C) Electron Transport Chain
D) Oxidative Phosphorylation
2. What is the net gain of ATP molecules produced directly from one glucose molecule during
glycolysis?
A) 1 ATP
B) 2 ATP
C) 4 ATP
D) 36-38 ATP

3. In which cellular organelle does the Krebs cycle primarily take place?
A) Cytoplasm
B) Endoplasmic Reticulum
C) Nucleus
D) Mitochondria

4. What is the final electron acceptor in the electron transport chain during aerobic respiration?
A) Oxygen
B) Carbon dioxide
C) Nitrogen
D) Hydrogen

5. Which molecule acts as the primary energy carrier in oxidative phosphorylation, contributing
to the majority of ATP synthesis?
A) NADH
B) FADH2
C) ATP
D) Pyruvate

Glycolysis

6. What is the net production of ATP molecules in glycolysis from the breakdown of one glucose
molecule?
A) 1 ATP
B) 2 ATP
C) 4 ATP
D) 38 ATP

7. Which enzyme catalyzes the conversion of glucose to glucose-6-phosphate in the first step of
glycolysis?
A) Phosphofructokinase
B) Hexokinase
C) Aldolase
D) Isomerase

8. What is the end product of glycolysis under aerobic conditions?

 A) Pyruvate
B) Lactic acid
C) Acetyl-CoA
D) Oxaloacetate

9. During glycolysis, NAD+ is reduced to which molecule?

A) NADH
B) NADPH
C) FADH2
D) ATP

10. Which step of glycolysis involves the conversion of phosphoenolpyruvate to pyruvate,

yielding ATP?
A) Step 1
B) Step 6
C) Step 7
D) Step 10

Electron Transport Chain (ETC)

11. Which molecules are the primary carriers of electrons in the Electron Transport Chain?
A) NAD+
B) FADH2
C) ATP
D) Cytochromes and coenzyme Q (ubiquinone)

12. In the Electron Transport Chain, where do the electron carriers transfer electrons to
ultimately generate ATP?
A) Matrix of the mitochondria
B) Inner mitochondrial membrane
C) Outer mitochondrial membrane
D) Cytoplasm of the cell

13. Which molecule is the final electron acceptor in the Electron Transport Chain during aerobic
respiration?
A) Oxygen
B) Carbon dioxide
C) Nitrogen
D) Hydrogen

14. Which complex of the Electron Transport Chain actively pumps protons (H+) across the
inner mitochondrial membrane?
A) Complex I (NADH dehydrogenase)
B) Complex II (Succinate dehydrogenase)
 C) Complex III (Cytochrome bc1 complex)
D) Complex IV (Cytochrome c oxidase)

15. What is the final result of the Electron Transport Chain in oxidative phosphorylation?
A) Synthesis of ATP
B) Production of NADH
C) Formation of pyruvate
D) Generation of FADH2