Group members and class no.

1. _____________________________________ 2. _____________________________________
kitty
Chan 11 ) venurs Chim ( O )

3. _____________________________________ 4. _____________________________________
[ 35 ]
Happysio ( 31 ) Bella
ung
5. _____________________________________ Date:______________________
51 10 12 之号w

Respiration /17
A. Background information
Yeast can carry out many biochemical reactions such as respiration which can be affected by factors
affecting any enzyme reaction. The factors affecting respiration in yeast are:
l Temperature l nutrient availability
l pH l build-up of waste materials
There are many experiments that can be done to investigate the impact of these factors on yeast
respiration.

B. Aim
To investigate the effect of temperature on yeast respiration.

C. Materials
Apparatus
l Test tube rack l Syringe
l 10% yeast suspension l Test tube
l Water bath (20oC, 40oC and 55oC) l Oil
l 5% glucose solution l Capillary tubing with rubber bung
l Timer l Boiling tube
l Spatula l Stand and clamp

D. Procedures
1. Label a boiling tube A and add 10ml of yeast suspension in the tube.
2. Use a syringe to place 10 ml glucose solution in tube A.
(Gently release the solution into the tubes by keeping the tip of the syringe in contact with the wall of
the tube so that the liquid runs down the wall of the tube without splashing and introducing air.)
3. Gently mix the yeast and glucose solution.
(Do NOT shake the tube.)
4. Add a thin layer of oil (roughly 1-2 cm thick) from the side of the boiling tube to cover the surface of the
mixture solution using a dropping pipette.
5. Place the boiling tube in the water bath for around 5 minutes. 5 minutes
6. Fill a clean test tube with some water.
7. Fit the capillary tubing with rubber bung into boiling tube A after it has left in 55oC water bath for
roughly 10 minutes.
8. Connect the other end of the capillary tubing into the test tube containing water.
9. Count the number of air bubbles produced in 5 minutes.
10. Repeat the experiment using boiling tube B and C and placed them in the water bath at 35oC and
20oC respectively.

E. Questions
1. What is the purpose of adding oil in the boiling tube? (1)

Prevent air going in. So that the yeast is having anaerobic respiration instead of aerobic respiration
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1
 2. Explain what kind of respiration was carried out in the experiment? (2)

Anaerobic respiration because the oil broke the oxygen from going in, so there will not be oxygen
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requirement for the yeast to take.
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3. How could you determine the type of gases that was produced? (1)
Using the lime water to test the gases. If the lime water change to milky, then shows that carbon dioxide was
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present

4. Apart from counting the number of bubbles produced, suggest an alternative way to measure the rate
of the reaction.
Include any apparatus you would use. (2)

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Put the yeast and glucose into test tube, then fill a thin layer of oil on them . Fit it with capillary tubing with rubber
bung . Put the measuring cylinder inside a tank of water and connect it with the capillary tubing. After that, measure the
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volume of gas in the measuring cylinder. ( picture on page 3 )

5. How would you set up a control for this experiment? (1)

Set up a experiment , which the yeast has been boiled to 140°F before starting the experiment.
…………………………………………………………………………………………………………………………….

6. Insert a photo of your experiment set up. (1)

⽃ 00 [
250c
( roomr temn peratlure )
的50 E

F. Results and Data

1. Draw a table to record the result from the above investigation. (5)

" 1111
Temperature 25°C 40°C 55°C

No. Of
Bubbles 40 279 105

2
ε
 2. Draw a graph of the results. (5)
0

.
1
50

Temperature
( °C)
3o

2 ←

0
60 OR 180 240 3O0

No of bubbles

3. Describe the data that you have collected from this investigation. (2)

时
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Before 40°C, the number of bubbles increase as the temperature increases. After 40°C the number of bubbles
started to decrease as the temperature increase
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G. Discussion and Conclusion

1. Discuss the factors that contribute to the outcome of the result being as it is. (2)

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The temperature will contribute to the outcome of the result As the temperature is lower than the optimum temperature
the ……………………………………………………………………………………………………………………………
activity of the yeast will react slower in the process of respiration, when it is higher than the optimum temperature
the yeast enzyme will be denatured which slows down the reaction. Therefore, the test in 40 degree Celsius is the
temperature that near the optimum temperature
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3
 2. Is there any error in your result? If so, what could you do to improve the experiment?

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Yes. For our first test in 40 degree Celsius, the no. of bubble has only 124 compare to the second test that has
……………………………………………………………………………………………………………………………
279 . It happened because our rubber tung isn’t fully fit inside the test tube which some carbon dioxide leaked
out. Thus, we should make sure the rubber tung is fit in tightly to improve the experiment.
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3. Describe how would you increase the reliability of your data without doing the experiment again on a
different day? (1)
…………………………………………………………………………………………………………………………….
Use sterile equipment, such as pipettes and flasks, to prevent contamination of the yeast with other microorganisms
that may interfere with the experiment.
…………………………………………………………………………………………………………………………….

Experiment

Organising and performing practical work

ü Perform the experiment properly /20

General laboratory skills

ü The experiment is carried out safely. /10
ü The work area is kept neat and tidy with all apparatus clean and put back to places
/10
where it was taken.
ü Finish the experiment within the time limit. /10

Total Mark:
Prelab /30
Experiment /30
Report /40
Total /100