ISOLATION AND PRODUCTION OF ANTIMICROBIAL COMPOUNDS OF MICROBIAL

ORIGIN AND SYNTHESIS OF ITS NANOPARTICLES TO ENHANCE ITS POTENCY

Khera Guneet Kaur, Suthar Zeel, Shalini Iyer and Rakeshkumar R. Panchal*
Department of Microbiology And Biotechnology, Gujarat University
panchalrrce@yahoo.com
introduction
An antimicrobial compound is a substance of natural, semi-synthetic or synthetic origin that kills or inhibits growth of microorganisms but causes little or no damage to
the host. Nanoparticles of dimensions ranging between 1-100 nm are designed and used for diagnostics, therapeutics and as a biomedical tool for research .Nanoparticles
have larger surface area to volume ratio which tends to pose higher antimicrobial activity. The nanoparticles synthesized using microbes enable the control of pathogens
with low toxicity and good biocompatibility.
Aims and objectives Observations and results
❖To isolate antimicrobial compound producing microorganisms from soil. ❖Results of comparison of antimicrobial activity of nanoparticles with standard antibiotics:
❖To screen the obtained isolates for its antimicrobial activity against Antibacterial (Streptomycin) and Antifungal (Itraconazole)
different pathogenic test organisms.
Zone of Inhibition (in mm)
❖To compare the potency of the antimicrobial compounds with standard
Sr Test Organism Standard Broth Nano Nano Pellet AgNO3
antibiotics. No Antibiotic Supernatant Supernatant Solution
❖To formulate the silver nanoparticles from the antibiotics and evaluate (50µg/mL)
FOR BACTERIA
its antimicrobial activity.
1 Bacillus subtilis 12 16 33 23 17
Isolation and screening of antimicrobial 2 Staphylococcus aureus 16 10 19 19 20
compounds producing microorganisms and its 3 Escherichia coli 12 11 20 20 23
production 4 Salmonella typhi 16 17 27 27 21
5 Aspergillus 10 11 20 20 18
Isolation of antimicrobial compound producing organisms from soil by
6 Penicillium 9 14 18 18 11
crowded plate technique on Nutrient agar plate and Potato Dextrose
7 Candida 9 10 17 17 19
Agar FOR ACTINOMYCETES
1 Micrococcus luteus 9 15 24 21 27
Plates were incubated at 25±3°C, 12 isolates were obtained which 2 Staphylococcus aureus 11 9 24 19 24
showed zone of inhibition 3 Klebsiella pneumoniae 19 9 16 23 22
4 Penicillium 8 10 14 13 14
5 Candida 11 9 13 11 14
3 isolates showing larger zone of inhibition were selected and screening FOR FUNGUS
was carried out against different test organisms 1 Bacillus subtilis 19 9 17 16 18
2 Staphylococcus aureus 16 10 21 19 26
All the isolates were having antibacterial and antifungal activity
3 Micrococcus luteus 15 13 18 23 24
4 Aspergillus 10 9 17 15 19
All 3 isolates were cultured in respective broth medium
ANTIBACTERIAL AND ANTIFUNGAL BIOASSAY FOR BACTERIA ANTIBACTERIAL AND ANTIFUNGAL BIOASSAY FOR ANTIBACTERIAL AND ANTIFUNGAL
ACTINOMYCETES BIOASSAY FOR FUNGUS

Bacteria Actinomycetes Fungi

Grown in N. broth for Grown in Glucose Grown in Potato

24 to 48 hours at 25 ± Aspargine Broth for Dextrose Broth for 48 to
2 °C for production of production of 72 hours for production
antimicrobial antimicrobial of antimicrobial
FORMULATION OF ANTIMICROBIAL FT-IR ANALYSIS OF SILVER NANOPARTICLES
compound compound (48-72h) compound SILVER NANOPARTICLES
ACTINOMYCETES FUNGUS
BACTERIA
BACTERIA
Broth centrifuged at 10,000 rpm for 20 minutes

Supernatant was used for checking antimicrobial activity by agar well ACTINOMYCETES
diffusion method

Antimicrobial activity was compared with standard antibiotics FUNGUS

Streptomycin and Itraconazole and potency was calculated
Formulation of silver nanoparticles of the
produced antimicrobial compounds ❖Comparison of Antimicrobial Activity of Nanoparticles with Standard Antibiotics
❖The supernatant of the harvested broth was used after centrifugation for
the formulation of silver nanoparticles. Standard Antibiotic
Zone of inhibition (mm)

❖10mM solution of AgNO3 was used.

Broth
❖7.5 mL of 10mM AgNO3 was added to 15 mL supernatant of all 3 isolates
into 100 mL flask and incubated in dark condition on magnetic stirrer for 24 Nano Particle

hours.
❖Change in colour was considered as formation of nanoparticles. The
absorption maxima was found to be between 400 nm to 435 nm by UV-vis
spectrophotometer after 24 hours of incubation.
❖Antimicrobial activity of silver nanoparticles was evaluated keeping conclusion
AgNO3 as control by agar well diffusion method. The antimicrobial compounds produced from 3 different isolates were found to be broad
references spectrum which were antibacterial as well as anti fungal and the silver nanoparticles were
❖Singh, H., Du, J., Singh, P., & Yi, T. H. (2018). Extracellular synthesis of silver nanoparticles by
formulated from the antimicrobial compounds enhances the potency by 61.8% , 67.3% and
Pseudomonas sp. THG-LS1. 4 and their antimicrobial application. Journal of pharmaceutical 78.04% in case of Bacteria, Actinomycetes and Fungus respectively. The characterization of
analysis, 8(4), 258-264. Silver Nanoparticles was done using UV- vis Spectrophotometer and FT-IR Spectroscopy.
❖Narasimha, G., Alzohairy, M., Khadri, H., & Mallikarjuna, K. (2013). Extracellular synthesis,
characterization and antibacterial activity of Silver nanoparticles by Actinomycetes isolative. acknowledgement
❖Hemath Naveen, K. S., Kumar, G., Karthik, L., & Bhaskara Rao, K. V. (2010). Extracellular
biosynthesis of silver nanoparticles using the filamentous fungus Penicillium sp. Arch. Appl. Sci. We are thankful to the teaching and non teaching staff of microbiology and Biotechnology Department
Res, 2(6), 161-167. for their help and support throughout the work.